• 대한기계학회논문집A
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• 제24권2호
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• pp.489-495
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• 2000

Stress and strain in continuum mechanics have a mathematical form of the second order tensor. it is well-known that the usefulness of tensor components could be explained in a relation with coordin ates system transformation and Mohr's circle could be easily used to make a coordinate system transformation of tensors. However, Mohr's circle is applied mainly to plane problems and its use to three dimensional cases is limitedly employed. In this paper, we propose a matrix and dyadic representation of stress and strain tensors which could equivalently replace the graphical representation of second order tensors. The use of the proposed representation might provide a valuable means for the educational respects as well as research view point.

• Journal of Ship and Ocean Technology
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• 제7권3호
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• pp.56-65
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• 2003

The hull monitoring systems of container ships with four long-base gages give enough information for identifying the hull girder loads such as bending and torsional moments. But such a load-identification for container ships has not been known. In this paper, a load-identification method is suggested in terms of a linear matrix equation that the measured strain vector equals to the multiplication of the transformation matrix and the desired strain component vector. The equation is proved to be mathematically complete by the property of positive-definite determinant of the transformation matrix. The method is applied to a hull stress monitoring system for 8100TED container ship during sea trial, and the estimated external loads illustrate reasonable results in comparison with the pre-estimated results. This moment decomposition concept has also been tested in real operation conditions. The typical phenomena over the Suez Canal illustrated very suitable results comparing with the physical understandings. Henceforth, one can effectively use the proposed concept to monitor the hull girder loads such as bending and torsional moments.

• Journal of Mechanical Science and Technology
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• 제19권7호
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• pp.1460-1468
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• 2005

Two possible shape memory processes, austenite to detwinned martensite transformation and twinned martensite to detwinned martensite transformation of a shape memory alloy have been modeled and examined. Eshelby's equivalent inclusion method with Mori-Tanaka's mean field theory is used for modeling of the shape memory processes of TiNi shape memory alloy reinforced aluminum matrix composite. The shape memory amount of shape memory alloy, plastic strain and residual stress in the matrix are computed and compared for the two processes. It is shown that the shape memory amount shows differences in a small prestrain region, but the plastic strain and the residual stress in the matrix show differences in the whole prestrain region. The shape memory process with initially martensitic state of the shape memory alloy would be favorable to the increase in the yield stress of the composite owing to the large compressive residual stress and plastic strain in the matrix.

• Journal of Welding and Joining
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• 제31권6호
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• pp.37-43
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• 2013

This study targets to make clear the connection between MS (Martensite start) point and welding shrinkage. We approved that a Martensite-transformed weldment may not yield state under low MS point, but also admitted the limitation of numerical calculation by inherent strain approach or thermal strain approach. Therefore, new thermal strain formulae during cooling stages were made. As a thermal strain is obtained by integrating thermal extension coefficient, a constant of integration should be decided. In our suggested formulae, the origin was based on totally remained austenite, and added strain from volume changes in Martensite transformation was based on totally transformed ferrite. Through the suggested methodology, It is verified that an MS point under a critical temperature can let weld shrinkage relax and the critical value can be obtained. For supporting this process, 15 weld-consumables were made, were tested by fillet type and were measured. As a result, a positive correlation between MS point and level of weld-distortion was obtained, but it was rather weak.

• 한국자원식물학회지
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• 제27권3호
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• pp.242-248
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• 2014

This study was conducted to establish an efficient transformation system by using somatic embryogenesis in an important Korean native conifer, Korean fir (Abies koreana). Embryogenic masses were induced from mature zygotic embryos of the Korean fir on Schenk and Hildebrandt medium, which was supplemented with thidiazuron. For genetic transformation, the embryogenic masses were co-cultivated with a disarmed Agrobacterium tumefaciens strain C58/pMP90 containing the plasmid vector pBIV10 or LBA4404 containing the plasmid vector MP90. Both vectors contain the kanamycin resistance and beta-glucuronidase (GUS) reporter genes. A total of 48 lines of embryogenic masses were selected on mLV medium containing $50{\mu}g/mL$ of kanamycin after 4 weeks of culture, following 3 days of co-cultivation with A. tumefaciens strain C58/pMP90 carrying pBIV10 (none of the lines was cultivated with strain LBA4404 carrying MP90). Quantitative real-time PCR was performed, and high levels of GUS transcripts were observed in the 48 putative transgenic lines; however, the control (non-transgenic line) showed negative results. Results of histochemical staining showed that the expression of the GUS reporter gene was observed in somatic embryos that developed from the embryogenic masses of all 48 lines. Stably transformed cultures were successfully produced by co-cultivation with A. tumefaciens strain C58/pMP90 carrying pBIV10 in Korean fir. Here, we have reported an Agrobacterium-mediated gene transfer protocol via somatic embryogenesis that may be helpful in developing breeding and conservation strategies for the Korean fir.

• Fibers and Polymers
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• 제1권1호
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• pp.18-24
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• 2000

The phase transformation of poly(trimethylene terephthalate) in crystalline state was simulated by atomistic modeling using molecular mechanics technique. The crystalline structure of PTT was successfully prepared using the well-defined unit cell structure of PTT and was satisfactorily verified by comparing that with the structure obtained from the x-ray diffraction experiments. The basic elastic properties were predicted in this study, showing that the crystalline structure of PTT is very pliable to the deformation at small strain. When the crystalline structure of PTT was stepwise deformed up to 50% of strain in chain direction under uniaxial extension condition, the change in dihedral angle of trimethylene unit from gg to tt conformation was accompanied with a large increase of stress, indicating that the phase transformation of PTT in crystalline state is difficult to occur.

• 한국재료학회지
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• 제12권8호
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• pp.608-612
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• 2002

The sliding wear behavior of an iron-base NOREM 02 hardfacing alloy was investigated in the temperature range of $25～250^{\circ}C$ under a contact stress of 103MPa (15ksi). With increasing temperature, the wear loss of Norem 02 in water increased slightly up to $180^{\circ}C$ at which Norem 02 showed the wear loss of 2.1mg. The wear resistance of Norem 02 resulted from the surface hardening due to the strain-induced phase transformation from austenite to $\alpha$'martensite during sliding wear. The wear loss of Norem 02 was smaller in water compared to air at same temperature because the water could be served as a sort of lubricant. The wear mode of NOREM 02 changed abruptly to severe adhesive wear at $190^{\circ}C$ and galling occurred above $200^{\circ}C$. It was caused that the strain- induced phase transformation took place below $180^{\circ}C$ while not above $190^{\circ}C$. Therefore, Norem 02 was considered to be inadequate at high temperature service area.

• 한국식물병리학회지
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• 제11권1호
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• pp.66-72
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• 1995

The coat protein (CP) gene of cucumber mosaic virus-As (CMV-As) strain was engineered for expression in the plant by using the cauliflower mosaic virus 35S transcript regulatory sequences. The CP gene was cloned into an Agrobacterium-derived binary vector. A chimeric gene was constructed by the cDNA of CMV-As CP and plant expression vector pBI121. The clone, pCMAS66, was first introduced into the phagemid vector pSPORT1 for situating sense orientation for translation and making restriction sites in order to re-introduce plant expression vector, pHI121. The resulting subclone pCASCP02 and plant expression vector pBI121 were treated with BamHI-SacI for excising the target gene and removing GUS gene, respectively. After Agrobacterium transformation by freeze-thaw technique, the clone, pCMASCP121-123 which contains sense orientation of the target gene, was selected and confirmed by restriction endonuclease analysis. The CMV-As CP gene was introduced into A. tumefaciens. The results on tobacco plant transformation with the vector system revealed that the system could be successfully introduced and showed high frequency of selection to putative transformations.

• 열처리공학회지
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• 제16권2호
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• pp.90-96
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• 2003

The effect of betatizing temperature on microstructure and transformation characteristics in a Cu-AI-Ni based pseudoelastic alloy fabricated by heated mold continuous casting by using metallography, XRD and calorimetry. The microstructure of cast rod betatized at $600^{\circ}C$ revealed a ${\beta}_1$ parent phase and a ${\gamma}_2$ phase precipitated along the casting direction. When the cast rod was betatized at the elevated temperature above $600^{\circ}C$, the ${\gamma}_2$ phase is completely dissolved into the matrix so that the volume fraction of the ${\gamma}_2$ phase was decreased. The parent phase was stabilized by betatizing at $600^{\circ}C$. However, the ${\beta}_1$ parent phase was transformed to both ${{\beta}_1}^{\prime}$ and ${{\gamma}_1}^{\prime}$ martensites with increasing betatizing temperatures above $600^{\circ}C$, while $M_s$ and $A_s$ temperatures were decreased. The stress-strain curves for compression test were not same with betatizing temperature; the stress-strain curves of the specimen betatized at $600^{\circ}C$ and $700^{\circ}C$ were linear but those of the specimen betatized at $800^{\circ}C$ and $900^{\circ}C$ were not linear.

• 한국미생물·생명공학회지
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• 제23권2호
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• pp.164-169
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• 1995

An endogenous cryptic plasmid, pBL1, which has been used to construct plasmid vectors for coryneform bacteria producing amino acids, was eliminated from Brevibacterium lactofermentum. The pBL1 was partially digested with Sau3AI and the resulting DNA fragments were subcloned into a suicide vector pEM1 which contains a kanamycin-resistant (km$^{r}$) gene. KM$^{r}$ B. lactofermentum transconjugants were obtained by conjugal transfer of the pEM1 derivatives containing pBL1 DNA fragments from Escherichia coli into B. lactofermentum. A km$^{r}$ transconjugant was analyzed to contain a plasmid pEB14, which occurred in vivo by homologous recombination between pBL1 and the conjugal-transferred plasmid. The pEB14 including the pEM1-derived km$^{r}$ gene was found to be lost concomitantly with km$^{r}$ phenotype, resulting in the construction of a pBL1-free strain of B lactofermentum. Based on transformation efficiencies and plasmid stability, the resultant pBL1- free strain is more useful than wild strain as a host cell for genetic manipulation. It could be concluded that foreign plasmid DNAs are efficiently isolated and analyzed from the pBL1-free strain because of the absence of endogenous pBL1 plasmid.