• Title/Summary/Keyword: transformation method

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A Study on the Tattoo Fashion (TATTOO의 패션성에 관한 연구)

  • 정은숙;김향수
    • Journal of the Korean Society of Costume
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    • v.53 no.2
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    • pp.153-169
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    • 2003
  • I think that apparel attaches importance to the degree of satisfaction of visual expression in one ornamental culture in which it is clothed on human body. whereas a tattoo attaches more importance to part of the expression of one's own identity in the same context. However. modern tattooing act was highlighted only as an element of act which was negative in the purpose and the degree of taste or which assumed the grotesque meaning of a particular group. away from pure concepts such as simple ornament or an amulet according to the flow of the world. A tattoo is gradually being seen as one of avant-garde methods of art again these days, and TATTOO LOOK is being popularized as street fashion as an effective method of one's own satisfied desires and peculiar expression of personality. I have got the following conclusions as the result of having studied on a tattoo which is establishing itself as another plastic art that is matchable with the fashion of dress and its ornaments in which human incessant instinctive desire for the ornament of body has been beyond nostalgia for the past and simple ornament which were pure in up-to-date modern society First, a tattoo is of personality. In that it solidifies one's own personality of another side by being transformed, one's own attraction can be transferred to a fashion tattoo with which one can emit one's own personality. Secondly. a tattoo is fashion. As a new body ornament called transformation by a tattoo was emerging, the fashionableness owned by a tattoo had in its area ample room for the fashionableness with which it can coexist with the culture of dress and its ornaments. Thirdly. a tattoo is popularized. TATTOO LOOK in which one's own satisfied desires and the expression of personality are properly applied is being recently transmitted easily everywhere in the world and popularized as street fashion. Fourthly, a tattoo is not depraved art. It's because of the point that a tattoo which had been recognized only as a decadent expression in which avant-garde elements of unknown nationality appeared mixed showed the possibility that it could establish itself as fashion art. If we go on studying and complementing the problems of individual sides about a tattoo also in the future, the infinite fashionableness for the elements called personality. sense and emotion owned by a tattoo suggested ample possibility as future industry to be highlighted.

Evaluation of Tissue Culture Efficiency in a Speed Breeding System for Stable and Sustainable Supported Wheat (Triticum aestivum) Immature Embryogenesis (안정적이며 지속적 밀(Triticum aestivum) 미성숙배 조직배양을 위한 스피드 브리딩 조건의 배양 효율 검정)

  • Lee, Geon Hee;Kim, Tae Kyeum;Choi, Chang Hyun;Kim, Jae Yoon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.365-376
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    • 2020
  • Immature embryogenesis is a useful process in wheat tissue culture, including transgenic technology, because of its high regeneration efficiency compared to that in other tissues. However, it is a very labor-intensive and time-restrictive method, because the preparation of immature embryos is limited to the optimal time after flowering. In this experiment, 'Speed Breeding', a breeding technique that accelerates breeding generation advancement by extending the photoperiod, was applied to the wheat variety 'Bobwhite'. A controlled growth room was constructed by adjusting the photoperiod (22-hour light/2-hour dark) using LED lights at temperature of 22℃. After vernalization of the Bobwhite seeds at 4℃ for 4 weeks, the seedlings were grown in a controlled growth room and a greenhouse to compare the heading date. In both conditions, calli were induced from immature embryos on the 11th day after flowering. After 4 weeks, the calli were transferred to a regeneration medium. Regeneration efficiencies under greenhouse conditions and Speed Breeding conditions were determined as 45.05% and 43.18%, respectively. Antioxidant enzyme activity and reference gene expression analysis were performed to confirm the presence of stress due to an extremely long-day photoperiod. As a result, the antioxidant enzyme activity was not distinguished from that of the greenhouse condition. The reference gene expression analysis revealed that the PsaA and CDC genes were highly expressed under the Speed Breeding condition. However, expression of PsbA was similar expression in both conditions. These results will provide useful information for the application of immature embryogenesis to the wheat transformation system.

A study on the laser surface hardening of SM 45C steel (SM 45C강의 레이저 표면경화처리에 관한 연구)

  • 나석주;김성도;이건이;김태균
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.11 no.1
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    • pp.53-62
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    • 1987
  • High power lasers provide a controllable and precise energy source in surface transformation hardening. A careful control of the process is needed in order that the surface layer of the material reaches the austenizing temperature, but that it does not melt. In order to achieve this the results of theoretical and experimental studies on the laser surface hardening of a medium carbon steel are described. A two-dimensional computer program, which can be used generally for the determination of transient temperature distributions in welding and heat treatment, was established on the basis of the finite element method. For the confirmation of the accuracy of the numerical analysis, a medium carbon steel (SM 45C) of 5mm thickness was heat-treated with a 1kW CW CO$_{2}$ laser machine, while the traverse speed and the distance from the focal point (defocused distance) were varied. Experimental and numerical results showed a similar tendency in correlations between the hardened zone shape and the process parameters. With increasing beam spot diameter the width and depth of the hardened zone increased for relatively small beam spot diameters, but decreased rapidly after reaching the maximum value, while with increasing traverse speed the width and depth of the hardened zone decreased monotonously. Too small beam spot diameters are to be avoided, since the surface melting would lower the surface hardness and produce an uneven surface which may be unacceptable because of the possible requirement for subsequent machining. It could be observed that for a given traverse speed and laser power input there exists a optimal range of the beam spot diameter, which produce a large width of the hardened zone but no melting on the surface.

Correlation Between Sasang Constitution and Heart Rate Variability in Won-ju Rural Population (원주 지역 주민들의 사상체질과 심박수변이도와의 상관성)

  • Kim, Soo-Yeon;Sun, Seung-Ho;Yoo, Jun-Sang;Koh, Sang-Baek;Park, Jong-Ku
    • The Journal of Internal Korean Medicine
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    • v.30 no.3
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    • pp.510-524
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    • 2009
  • Objective : This study was designed to find the correlation between Sasang Constitution and heart rate variability(HRV). Method : There were 665 subjects (280 men and 385 women), between 39 and 72 years old. in a rural community. Sasang Constitution was diagnosed by a Sasang constitutional specialist using PSSC (Phonetic System for Sasang Constitution), face and tongue photo and checkup-list. A structured-questionnaire was used to assess the general characteristics. HRV was recorded using SA-2000 (medi-core). HRV was assessed by time domain and by frequency domain analysis. Metabolic syndrome was defined on the basis of clustering of risk factors, when three or more of the following cardiovascular risk factors were included : blood pressure, fasting blood sugar, triglyceride HDL-cholesterol, and abdominal obesity (waist). Because of the skewness of the data, logarithmic transformation was performed on the absolute units of the spectral components of HRV, and the resulting logarithmic values and normalized units were compared between the groups by a logistic regression. The 95% confidence interval (CI) of the odds ratio was used and calculated from the data laid out for a cross sectional study. Results : 1. Odds ratios of Taeeumin and Soeumin in female adults below 60 years old were significantly lower than that of Soyangin in LF norm and LF/HF ratio. Odds ratios of Taeeumin and Soeumin in female adults below 60 years old were significantly higher than that of Soyangin in HF norm. 2. There was no significant correlation between HRV and Sasang Constitution in female adults from 60 years old and over. 3. There was no significant correlation between HRV and Sasang Constitution in male adults. Conclusion : There is a statistically significant correlation between the HRV and Sasang Constitution. There is a tendency of increase in the sympathetic activity in Soyangin. There is a tendency of decrease in the parasympathetic activity in Taeeumin and Soeumin.

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Effects of Tobacco-Specific Carcinogen on Protein Kinase C Isoforms (흡연특이성 발암물질이 특정 Protein Kinase C Isoform에 미치는 영향)

  • Kang, Hyung-Seok;Ko, Moo-Sung;Park, Ki-Sung;Lee, Sub;Jheon, Sang-Hoon;Kwon, Oh-Choon
    • Journal of Chest Surgery
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    • v.36 no.9
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    • pp.666-673
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    • 2003
  • Cigarette smoking is the leading cause of the lung cancer. However, mechanism of action underlying the carcinogenesis in the lung still remains to be elucidated. The present study attempted to look into the carcinogenic potential of tobacco-specific nitrosamine, NNK (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone) and the effects of protein kinase C (PKC) isoforms in an immortalized human epithelial cell model. Material and Method: Immortalized human epithelial cells were exposed with NNK and examined for its carcinogenic potential as measured by saturation density, soft-agar colony formation, and cell aggregation assay. The specific isoform of PKCs involved in the cellular transformation was analysed through western blot with monoclonal antibody and measured separately in cytosolic fraction and membrane fraction. Result: Human epithelial cells exposed with NNK showed prominent carcinogenic potential in saturation density, soft agar colony formation, and cell aggregation assay. PKC isoform analysis results are as follows: PKC- $\alpha$ showed significant translocation of protein levels from cytosolic fraction to membrane fraction, as analyzed by immunoblot. PKC- $\varepsilon$ showed a dose-dependent increase of translocation. PKC- λ was not affected by NNK treatment. Conclusion: The study demonstrated that there was a certain specificity in the patterns of isoform induction following chemical carcinogen exposure. Thus, it is suggested that identification of specific isoform be a clue to find target molecules in the carcinogenesis.

Cellulose Structures of Primary and Secondary Tissues in Pinus densiflora S. et Z. (소나무재의 1차조직과 2차조직 세포벽 중의 셀룰로오스 구조)

  • Kim, Nam-Hun;Lee, Kee-Young
    • Journal of the Korean Wood Science and Technology
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    • v.29 no.1
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    • pp.60-67
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    • 2001
  • The microscopic characteristics and cellulose structures of primary and secondary tissues in Pinus densiflora S. et Z. were examined. Cells of primary tissue in cross section showed an irregular arrangement and round shape. Fiber lengths were 200 to $250{\mu}m$ in primary tissue, and 1,500 to $1,600{\mu}m$ in secondary tissue. Cell diameters in primary tissue were larger than those in secondary tissue; 40 to $50{\mu}m$ in former and 10 to $20{\mu}m$ in latter. Crystallite width and d-spacing of (200) in both tissues did not show any significant differences. However, crystallinity indices by Segal's method showed significant differences as 23% in primary tissue and 35% in secondary tissue. In the orientation of cellulose microfibril, primary tissues had a random pattern, whereas, secondary tissues presented an oriented pattern with 20 to 30 degree. The cellulose crystalline of primary tissue was easily transformed into cellulose II by mercerization, but that of secondary tissue hardly transformed. It is considered that the difference of crystal transformation in both tissues could be caused by the difference of lignification.

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Prevalence and risk factors of subclinical bovine mastitis in some dairy farms of Sylhet district of Bangladesh

  • Kahir, Md. Abdul;Islam, Md. Mazharul;Rahman, A.K.M. Anisur;Nahar, A.;Rahman, Md. Siddiqur;Son, Hee-Jong
    • Korean Journal of Veterinary Service
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    • v.31 no.4
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    • pp.497-504
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    • 2008
  • A cross-sectional study was undertaken to report prevalence and to identify risk factors of subclinical mastitis of dairy cattle in Sylhet district of Bangladesh. Among 325 dairy farms of the district 12 farms(3.7%) were selected conveniently for this study. All the dairy cows of the 12 farms were selected for sample collection. Fresh milk samples from each of the selected dairy cows were collected aseptically in separate sterilized test tube as RF, RH, LF and LH quarter of the udder. Rapid modified White Side Test(WST) was used to detect subclinical mastitis(SCM). Results of WST and data derived from filled in questionnaire were entered in Microsoft Excel 2003 and transferred to $STATA^{(R)}$, version 8.0/Intercooled(Stata Corporation, Texas, USA, 2003). The overall prevalence of SCM and its distribution in different categories of variables in cow and their exact binomial 95% confidence intervals were calculated in $STATA^{(R)}$. Simple bivariable associations among independent variables were investigated by $x^2$ test in $STATA^{(R)}$. Multiple logistic regression analysis with backward elimination method was used to identify risk factors of SCM. To identify significant variation in quarter SCM, linear regression analysis was performed after arcsine transformation of the data. The overall prevalence of SCM found in this study is 54%. Dairy cows with teat lesions had significantly increased SCM(OR=12342, P value=0.000, 95% CI=762, 199798) than others without teat lesions. The Holstein Friesian X Jersey X Sahiwal breed has significantly decreased(OR=0.18, p=0.03, 95% CI 0.04, 0.85) SCM than other breeds. The prevalence of SCM found in this study is in agreement with others. The injury in the teat increases the probability of getting infected with microbes and thereby mastitis. If the prevalence of teat lesion can be decreased the probability of subclinical mastitis will also be decreased. The negatively associated Holstein Friesian X Jersey X Sahiwall breed may help in planning mastitis control program if this finding can be validated by a more powerful case-control or cohort study design.

A Study on the Libraries' Competitiveness Reinforcement Measures through SWOT Analysis : Based on the Case Study of University A's Library (SWOT 분석을 통한 도서관의 경쟁력 제고 방안에 관한 연구 - A대학교 도서관의 사례를 중심으로 -)

  • Noh, Dong-Jo
    • Journal of the Korean Society for Library and Information Science
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    • v.40 no.1
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    • pp.335-351
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    • 2006
  • For a library to be run more efficiently, its internal strengths and weaknesses, external opportunities and threats must be determined for forecasting on the basis of the diagnosis of the internal and external environment and situations, and optimum strategies and measures responsively deducted and utilized. Therefore, I had analyzed the strategic competition of university A's library using SWOT analysis, which has recently been spotlighted as an effective strategic competition analysis method. The conclusions thus obtained are as explained below. Firstly. the strengths of university A's library are the professional qualifications of the employees. work efficiency based on systematic operative plans and division of work load, and provision of high quality service following the establishment of the digital library. Secondly, its weaknesses are the insufficiency and limits of the usable resources, the lack of progressive future operation plans and systems, and the deficiency of a positive service mind. Thirdly, the opportunities are the possibility for new developments in accordance with the digital information environment. reinforcement of the operative efficiency throughout expanding its cooperation with external organizations and other libraries, and its transformation as the local library hub by opening up to the public. Fourthly, the threats are the lack of the school authorities' acknowledgement. and the degradation of the library's status in accordance, the users' various requests for information and advanced professional service according to the rapidly changing information environment. and the restriction of the library service based on the copyright law. Fifthly, as a result of analyzing the strategic competition of university A's library, measures such as the reinforcement of digital service by acquiring digital specialists. provision of extended service through active cooperation with external institutes and libraries. development and provision of differentiated service in accordance with the reinforced copyright law were proposed to improve the library's future competitiveness.

Expression of p27kip1 Protein in Astrocytic Tumors (성상세포종에서의 p27kip1 단백의 발현)

  • Kim, Dae Yong;Son, Hyun Jin;Chung, Myoung Ja;Kang, Myoung Jae
    • Journal of Korean Neurosurgical Society
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    • v.30 no.4
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    • pp.443-450
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    • 2001
  • Objective : The cyclin-dependent kinase inhibitor $p27^{kip1}$ protein is a negative regulator of the cell cycle, and its degradation is required for entry into the S phase. Loss of $p27^{kip1}$ expression has been reported to be associated with aggressive behavior in a variety of tumors of epithelial and lymphoid origin. However, its association with various astrocytic tumors has not been clearly demonstrated. We studied to investigate the relationship of $p27^{kip1}$ expression with the biological behavior of astrocytic tumors in addition to study on the role of $p27^{kip1}$ in the tumorigenesis of these tumors. Patients and Methods : From 1990 to 1998, a total of 29 astrocytic tumor of all grades obtained by operative resection were included for evaluation. We studied the expression of $p27^{kip1}$ protein immunohistochemical assay in astrocytic tumors and compared the findings with the clinicopathologic parameters. Immunohistochemical staining was performed on formalin-fixed paraffin-embedded sections by the avidin-biotin-peroxidase complex method. According to WHO classification, all cases were divided into astrocytomas(4 cases), anaplastic astrocytomas(9 cases), and glioblastomas(16 cases) by 3 pathologists. Clinical information was obtained from medical records, and others such as location and size of tumors from imaging studies. Results : Mean $p27^{kip1}$ protein labeling indexes(LI, mean${\pm}$standard deviation) of astrocytomas, anaplastic astrocytomas, and glioblastomas were $80.6{\pm}9.1$, $63.6{\pm}21.0$, and $28.9{\pm}18.7$, respectively, and were inversely correlated with grade of glial tumors(p<0.0001). Mean $p27^{kip1}$ protein LI in the recurrent group was lower than that in the nonrecurrent group, but there was no significant difference statistically(p=0.464). Additionally, $p27^{kip1}$ protein expression did not show any significant relationship to other prognostic factors such as age(p=0.1643), tumor size(p=0.8), or location(p=0.8). Conclusion : These results suggested that reduced expression of $p27^{kip1}$ protein may play a important role in the malignant transformation process of astrocytic tumor cells.

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Establishment Aerobic Soil Metabolism System Using [14C]Butachlor ([14C]Butachlor를 이용한 호기성 토양대사 시험법 확립)

  • Kim, Ju-Hye;Kim, Jong-Hwan;Kim, Dae-Wook;Lee, Bong-Jae;Kim, Chan Sub;Ihm, Yangbin;Seo, Jong-Su
    • The Korean Journal of Pesticide Science
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    • v.18 no.4
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    • pp.258-268
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    • 2014
  • The test method of aerobic transformation in soil has established based on international test guideline (OECD TG 307). And then, the case study was conducted with [$^{14}C$]butachlor. Butachlor is commonly used herbicide in Korea. [$^{14}C$]Butachlor was treatrd $6.83mgKg^{-1}$ in loamy soil. The treated soil was incubated in flow-through system for 60 days. The mass balance of applied radioactivity (AR) ranged from 91.1 to 95.5% and from 93.0% to 97.7% for non-sterile and sterile soils, respectively. In non-sterile soil, the concentration of [$^{14}C$]butachlor was declined from 94.4% AR at 0 day to 8.4% AR at 60 days after treatment. 2-Chloro-2',6'-diethylacetanilide was the major degradation product detected in soil extract. The calculated $DT_{50}$ and $DT_{90}$ of butachlor were 10.4 days and 34.6 days, respectively. $^{14}CO_2$ and non-extractable soil residue were increased up to 3.5% and 43.5% AR at 60 DAT. There is no significant decrease of the [$^{14}C$]butachlor through the incubation period in sterile soil.