• Applied Biological Chemistry
• /
• v.40 no.3
• /
• pp.173-177
• /
• 1997

Bacillus licheniformis 9945a releases a natural ${\gamma}-poly(glutamic\;acid)({\gamma}-PGA)$ into fermentation broth and shows a mucoid phenotype on the solid agar medium. Transformation of mucoid cells of Bacillus species has not been simple and straightforward. The transpositional activity of Tn10 in B. licheniformis also has not been own either. Thus, a spontaneous non-mucoid derivative of the B. licheniformis was obtained first. Shuttle vector pHV1248 containing mini-Tn10 was introduced into the non-mucoid derivative by the method of protoplast transformation. The resulting transformant was reverted to the wild mucoid phenotype, and then mutated randomly with the mini-transposon by heat induction. Auxotrophs requiring arginine, lysine, or tryptophan were isolated by replica plating method. Southern blotting and DNA-DNA hybridzation analysis showed that these auxotrophs were generated by mini-Tn10 insertion into the chromosomal DNA. This method of transformation and mutation using pHV1248 would be useful for the generation of diverse mutants of B. licheniformis 9945a.(Received January 24,1997; accepted March 10, 1997)

• Journal of the Korean Association of Geographic Information Studies
• /
• v.7 no.4
• /
• pp.24-33
• /
• 2004

Advances in remote sensing technologies are resulting in the rapid increase of the number of spectral channels, and thus, growing data volumes. This creates a need for developing faster techniques for processing such data. One application in which such fast processing is needed is the dimension reduction of the multispectral data. Principal component transformation is perhaps the mostpopular dimension reduction technique for multispectral data. In this paper, we discussed the processing procedures of principal component transformation. And we presented and discussed the results of the principal component transformation of the multispectral data. Moreover principal components image data are classified by the Maximum Likelihood method and Multilayer Perceptron method. In addition, the performances of two classification methods and data reduction effects are evaluated and analyzed based on the experimental results.

• Journal of the Korea Society of Computer and Information
• /
• v.22 no.2
• /
• pp.97-104
• /
• 2017

In semantic information retrieval, we first need to build domain ontology and second, we need to convert the users' search keywords into a standard query such as SPARQL. In this paper, we propose a method that can automatically convert the users' search keywords into the SPARQL queries. Furthermore, our method can ensure effective performance in a specific domain such as law. Our method constructs the keyword history ontology by associating each keyword with a series of information when there are multiple keywords. The constructed ontology will convert keyword history ontology into SPARQL query. The automatic transformation method of SPARQL query proposed in the paper is converted into the query statement that is deemed the most appropriate by the user's intended keywords. Our study is based on the existing legal ontology constructions that supplement and reconstruct schema and use it as experiment. In addition, design and implementation of a semantic search tool based on legal domain and conduct experiments. Based on the method proposed in this paper, the semantic information retrieval based on the keyword is made possible in a legal domain. And, such a method can be applied to the other domains.

• Archives of design research
• /
• v.19 no.1 s.63
• /
• pp.99-108
• /
• 2006

The ways in which twentieth-century designers have transformated com mom objects into creative new visions are seemingly infinite yet they conform to certain basic formulas. A remarkable method in those basic formulas can be a transformation. The purpose of this paper is to question what transformation is. After viewing and researching many number of examples of design and how designer dealt with issues of transformation, the result of this paper is as follows. Functional elements such as bases, handles, and openings are inverted in meaning or exaggerated in scale. Designers play upon what we normally expect from metal, glass, and other standard materials; making hard materials seem soft, making soft materials seem hard, transferring techniques appropriate for one material to another. Designer employed an endless variety of plays on functional elements and questioned the nature of materials I concluded that 'transformation in design is the main method of the creative working process for the designer.

• Spatial Information Research
• /
• v.11 no.4
• /
• pp.481-491
• /
• 2003

This study aims at generating a continuous map by coordinates transformation between cadastral maps with different surveying origins. The continuous cadastral map is useful in various fields of GIS. For this purpose, an experimental study was conducted at Osan-Si, Kyonggi Province in cooperation with related institutions. In this study, three control point zone, large, medium, and small zone were to!;ted. For each control point, the currently used data were compared with the data at the surveyed time. About coordinate transformation method, we tested Helmert, Affine, and Polynomial methods which are the most representative among 2-dimensional coordinate transformations. These three transformation methods were evaluated according to variation of transformed parcel shape and agreement with neighboring areas. As the result of the evaluation, Affine transformation in large zone is the most appropriate coordinate transformation method fer Osan-Si.

• Spatial Information Research
• /
• v.22 no.1
• /
• pp.35-43
• /
• 2014

The Triangle Mesh Warping method is suggested and applied in coordinate transformation to world geodetic system in this study. The common points of Uiwang city are used to compare the transformation accuracy of the suggested methods with existing national coordinate transformation methods. As a result, the Triangle Mesh Warping method was satisfied with accuracy criteria for positioning on a map larger than scale 1/1,000 with smaller number of common points and without distortion modeling. Additionally, in case of Guri and Pyeongtaek city that established the World Geodetic System, the suggested method generates the result of transformation accuracy better than 5cm. Based on the test, it was found that the suggested method improves the problem of securing many common points and reduces the problem of mis-match between the transformed data of adjacent areas. Accordingly, for transformation of large-scale topographic map, cadastral map, GIS DB and serial cadastral map to the World Geodetic System, it is judged that the Triangle Mesh Warping would be a good method for economical efficiency and accuracy using by minimum common point.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.32 no.4D
• /
• pp.407-419
• /
• 2012

It is difficult to obtain the accurate and homogeneous height information over the whole Korea due to the effect of different vertical datums have been divided into land and sea part. In this study, we tried to unify the different vertical datums using the precise spirit leveling between TBM (tidal bench mark) and BM (bench mark) in order to solve the problems caused by different vertical datums. For this, the vertical datum offsets at observed points which were calculate from leveling results and then transformation model of vertical datum will be modelled using calculated offsets along the coastal line. For suggesting the precise modelling method to vertical datum transformation, we analyzed results from various interpolation methods such as Spline and LSC method. As the results from analysis, the LSC method combined with 4-parameters trend model is more suitable for modelling the offsets between vertical datums. The final transformation model of vertical datum using the combination of LSC and 4-parameter model which provides the transformation accuracies of ${\pm}10.4cm{\sim}14.8cm$ level. And, the software for vertical datum transformation that was also developed using the final model in order to convert the height information included in various spatial data effectively. Therefore, the transformation model between vertical datums of land and sea part, which is developed in this study, is expected to minimize the confusion caused by mismatch of height information in the use of spatial data, and it also can be minimize economic and time losses in various application fields such as coastal development project, coastal disaster prevention, etc.

• Korean Journal of Remote Sensing
• /
• v.17 no.1
• /
• pp.85-97
• /
• 2001

The KT(Kauth-Thomas) and IHS(Intensity-Hue-Saturation) transformation techniques were introduced and compared to investigate fire-scarred areas with single post-fire Landsat 7 ETM+ image. This study consists of two parts. First, using only geometrically corrected imagery, it was examined whether or not the different level of fire-damaged areas could be detected by simple slicing method within the image enhanced by the IHS transform. As a result, since the spectral distribution of each class on each IHS component was overlaid, the simple slicing method did not seem appropriate for the delineation of the areas of the different level of fire severity. Second, the image rectified by both radiometrically and topographically was enhanced by the KT transformation and the IHS transformation, respectively. Then, the images were classified by the maximum likelihood method. The cross-validation was performed for the compensation of relatively small set of ground truth data. The results showed that KT transformation produced better accuracy than IHS transformation. In addition, the KT feature spaces and the spectral distribution of IHS components were analyzed on the graph. This study has shown that, as for the detection of the different level of fire severity, the KT transformation reflects the ground physical conditions better than the IHS transformation.

• Membrane and Water Treatment
• /
• v.4 no.1
• /
• pp.1-9
• /
• 2013

The process of electromembrane transformation of L-lysine monohydrochlorides into their zwitterionic form in four- and two-chamber electrodialysis apparatus was investigated. The process of transformation at various concentrations of lysine monohydrochloride (0.1-0.6 mol.L-1) was studied and it was established that at the optimum density of current optimal concentrations of lysine hydrochloride during electrodyalisis was in the range of 0.2-0.4 mol.L-1. It was determined that the process of total transformation was accomplished when pH of the lysine solution achieved 10. Changes of concentrations of $Cl^-$ ions and lysine diffused into the neighboring chamber were determined depending on the time. The method developed by us allows adjusting the removal coefficient of $Cl^-$ ions during transformation to a maximal value, the losses of lysine diffused into the next chamber after its return to the technological cycle being less than 1.0 %. The specific energy consumption during the process of transformation in two- and four-chamber electrodialyzers was 0.19 and 0.205 A.h.kg-1 and the current efficiency was 75.9 and 73.1 %, correspondingly. Study of the process of electromembrane transformation allowed obtaining zwitterionic form of L-lysine from L-lysine monohydrochloride with minimal reagent and energy consumption.

• Journal of Korean Society for Quality Management
• /
• v.50 no.1
• /
• pp.35-42
• /
• 2022

Purpose: The purpose of this study is to examine the characteristics and problems of existing research through various literature investigations on the existing R&D process. Implications are identified through the investigation of existing studies and the characteristics of the digital transformation era are analyzed. We research and propose a new R&D process suitable for the new era of digital transformation. Methods: The research method investigates various literature precedent studies on the existing R&D process and the methods used by companies. Research new R&D processes that reflect the characteristics of the digital transformation era and develop new related models. Results: In the era of digital transformation, the parallel R&D process is being reorganized to respond quickly to the demands of rapid market changes. In order to respond quickly to various customers, the R&D process can respond flexibly in a parallel structure. Conclusion: Along with the development of the R&D process, additional research on indicators and evaluation models that can evaluate them fairly and flexibly is needed. Further research is needed to determine whether the R&D process suitable for the digital transformation era is operating properly.