• 한국농촌건축학회논문집
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• 제6권2호
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• pp.65-78
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• 2004

Transformation of housing space is the most accurate physical trail to understand main needs from residents. The scope of the study targeted the 7 si & gun districts in Gyeongsangnam-do, this study is designed to consider the characteristics of space transformation in rural apartment houses and provide basic materials for the development of rural apartment houses. The results of the study are as follow. First, such spaces as corridor and stairway are transformed into storages and outside the houses are installed tap water facility, bicycle storage. and toilet. Second, in autumn seasons when crops and grains are harvested, parking lot and rooftop are used as the space drying such agricultural products. Third, space transformation within the houses has been different depending on family type and main businesses in terms of function and time to seek efficiency and stability of residence. Fourth, rural residents concentrate on a simple change of space usage and replacement of ageing or dilapidated equipment and facilities rather than space transformation incurring more financial burden.

• 동아시아경상학회지
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• 제9권4호
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• pp.103-111
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• 2021

Purpose - We attempt to provide a background, a review of current development of the digital technologies and their effect on the digital transformation process, and then offer our recommendations for identifying and taking strategic actions to exploit them. More importantly, we also aim to offer important recommendations for policymakers and practitioners how to better advance the innovation system by facilitating the development of digital technologies and take advantage of improvements in digital technologies. Research design, data, and methodology - This study undertakes an extensive and critical review of the digital transformation literature and identifies several important issues with relevant practical examples of best practices in the transition to digital transformation process. Results - This paper finds that digital technologies with strong technological backing have brought technological spillovers to the economy while also generating shocks. Conclusion - To maintain a healthy and developing market order, it is necessary for governments to innovate regulatory models. This study offers several important suggestions for government regulatory innovation that we believe should help to better deploy digital transformation initiatives and enhance the efficiency and effectiveness of government regulation in contributing more to the high-quality and healthy development of the digital economy.

• 한국항만경제학회지
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• 제31권1호
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• pp.85-110
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• 2015

본 논문에서는 아시아 컨테이너항만들 간의 클러스터링 추세를 분석하기 위해서 퍼지(평균지수변환)DEA모형과 교차효율성모형에 대해서 이론적으로 설명하고, 아시아 38개 컨테이너항만들의 12년간 자료를 4개의 투입요소(선석길이, 수심, 총면적, 크레인 수), 1개의 산출요소(컨테이너화물처리량)를 이용하여 국내항만(부산, 인천, 광양항)들이 어떤 항만들과 클러스터링 해야만 하는지에 대한 측정방법을 실증적으로 보여 주고 분석하였다. 실증분석의 주요한 결과는 다음과 같다. 첫째, 퍼지(평균지수변환)DEA모형에 의한 클러스터링 추세분석에서 국내항만들은 클러스터링을 통해서 효율성을 증대[부산항(56.29%), 인천항(57.96%), 광양항(66.80%)]시 킬 수 있는 것으로 나타났다. 둘째, 원자료를 이용한 교차효율성 모형을 이용한 클러스터링분석에서는 부산항(홍콩, 코오베, 마닐라, 싱가포르, 카오슝, 림찬방, 방콕항), 인천항(아카바, 담만, 카라치, 모하메드 빈 오아심, 다바오), 광양항(담만, 요코하마, 나고야, 킬롱, 카오슝, 방콕항)과 각각 클러스터링을 해야만 하는 것으로 나타났다. 셋째, 퍼지(평균지수변환)DEA모형에 교차효율성 모형을 접목시킨 모형에서는 부산항은 71.38%, 인천항은 103.89%, 광양항은 168.55% 증가가 이루어 졌다. 넷째, 효율성 순위를 검정한 윌콕슨부호순위검정에서는, 세 가지 모형사이의 효율성 순위에 대해서는 약 66%-67% 수준에서 순위에 차이가 없는 것으로 나타났다. 본 논문이 갖는 정책적인 함의는 첫째, 항만정책입안자들이 본 연구에서 사용한 두 가지 모형과 접목시킨 모형을 항만의 클러스터링 정책에 도입하여 해당항만이 발전할 수 있는 전략을 수립하고 이행해 나가야만 한다는 점이다. 둘째, 본 논문의 실증분석결과 국내항만들의 참조항만, 클러스터링항만들로서 나타난 아시아항만들에 대하여, 그들 항만들의 항만개발, 운영에 대한 내용을 정밀하게 분석하고 도입하여 실시하는 것이 필요하다.

• 한국초지조사료학회지
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• 제29권3호
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• pp.165-170
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• 2009

신품종 페레니얼 라이그라스를 개발할 목적으로 Agrobacterium을 이용한 효율적인 형질전환 체계를 확립하였다. 페레니얼 라이그라스 성숙종자 유래의 캘러스를 pCAMBIA 3301을 가지는 Agrobacterium EHA105를 이용하여 감염시킨 후 형질전환을 실시하였다. Agrobacterium을 이용한 형질전환에 있어서 중요한 인자로 작용하는 몇 가지 요인에 대한 형질전환 효율을 GUS 유전자의 발현정도로 조사하였다. Agrobacterium 감염시에 현탁배지와 공동배양 배지에 100 ${\mu}M$의 acetosyringone(AS)을 첨가해 주었을 때 형질전환 효율이 증가되었으며, 공동배양기간을 5일까지 증가시켰을 때 형질전환 효율이 증가되었다. 10mg/L의 phosphinothricin(PPT)이 첨가된 선발배지에서 살아남은 캘러스로부터 정상적인 식물체가 재분화 되었으며 이들 형질전환체를 GUS 염색과 PCR 분석을 실시하여 본 결과 발현벡터의 GUS 유전자가 형질전환 식물체의 genome에 성공적으로 도입되었음을 확인할 수 있었다. 또한 RNA 수준에서 도입 유전자의 발현을 확인한 결과 도입 유전자가 안정적으로 발현하는 것을 확인하였다.

• 한국환경농학회지
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• 제40권3호
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• pp.186-193
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• 2021

BACKGROUND: Before generating transgenic plant using the CRISPR-Cas9 system, the efficiency test of sgRNAs is recommended to reduce the time and effort for plant transformation and regeneration process. The efficiency of the sgRNA can be measured through the transient expression of sgRNA and Cas9 gene in tomato cotyledon; however, we found that the calculated efficiency showed a large variation. It is necessary to increase the precision of the experiment to obtain reliable sgRNA efficiency data from transient expression. METHODS AND RESULTS: The cotyledon of 11^th, 15^th, 19^th, and 23^rd-day-old tomato (Solanum lycopersicum cv. Micro-Tom) were used for expressing CRISPR-Cas9 transiently. The agrobacterium harboring sgRNA for targeting ALS2 gene of tomato was injected through the stomata of leaf adaxial side and the genomic DNA was extracted in 5 days after injection. The target gene edition was identified by amplifying DNA fragment of target region and analyzing with Illumina sequencing method. The target gene editing efficiency was calculated by counting base deletion and insertion events from total target sequence read. CONCLUSION: The CRISPR-Cas9 editing efficiency varied with tomato cotyledon age. The highest efficiency was observed at the 19-day-old cotyledons. Both the median and mean were the highest at this stage and the sample variability was also minimized. We found that the transgene of CRISPR-Cas9 system was strongly correlated with plant leaf development and suggested the optimum cotyledon leaf age for Agrobacterium-mediated transfection in tomato.

• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.674-680
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• 2013

Yeast Dekkera/Brettanomyces bruxellensis is probably the most common contaminant in wineries and ethanol production processes. The considerable economic losses caused by this yeast, but also its ability to produce and tolerate high ethanol concentrations, make it an attractive subject for research with potential for industrial applications. Unfortunately, efforts to understand the biology of D. bruxellensis and facilitate its broader use in industry are hampered by the lack of adequate procedures for delivery of exogenous DNA into this organism. Here we describe the development of transformation protocols (spheroplast transformation, LiAc/PEG method, and electroporation) and report the first genetic transformation of yeast D. bruxellensis. A linear heterologous DNA fragment carrying the kanMX4 sequence was used for transformation, which allowed transformants to be selected on plates containing geneticin. We found the spheroplast transformation method using 1M sorbitol as osmotic stabilizer to be inappropriate because sorbitol strikingly decreases the plating efficiency of both D. bruxellensis spheroplast and intact cells. However, we managed to modify the LiAc/PEG transformation method and electroporation to accommodate D. bruxellensis transformation, achieving efficiencies of 0.6-16 and 10-20 transformants/${\mu}g$ DNA, respectively. The stability of the transformants ranged from 93.6% to 100%. All putative transformants were analyzed by Southern blot using the kanMX4 sequence as a hybridization probe, which confirmed that the transforming DNA fragment had integrated into the genome. The results of the molecular analysis were consistent with the expected illegitimate integration of a heterologous transforming fragment.

• 응용통계연구
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• 제22권1호
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• pp.189-195
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• 2009

A dynamic graphical procedure is suggested to estimate optimal response transformation parameter and a curvature function of covariates in the regression model. Augmented partial residual plot is chosen for specifying a curvature. The proposed method is compared with a different approach (Soo, 2007) and is investigated efficiency by applying it to the real and the artificial data. The method is also extended to the 3D graphical situations.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 춘계학술대회
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• pp.181-181
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• 2002

• Journal of Microbiology
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• 제37권2호
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• pp.51-58
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• 1999

Using yeast, Saccharomyces cerevisiae, and the integrate vector system, we have isolated and characterized an autonomously replicating sequence (ARS) from Aspergillus nidulans. The DNA fragment, designated ANR1, is 5.0 kb in size and maintained free from the chromosome in S. cerevisiae. The YIplac211-ANR1 recombinant plasmid, which consists of sequences derived from the yeast integrative vector YIplac211 and 5.0 kb ANR1 fragment, showed a 104-fold enhancement in transformation efficiency over that found for YIplac211, and was easily recovered from the transformed yeast. Genetic analysis of transformants showed that YIplac21-ANR1 could be over 96% cured when cultured over 20 generations in complete medium and thus suggests that this sequence is mitotically unstable. In A. nidulans, recombinant plasmid PILJ16-4.5 which carries the 4.5 kb EcoRI fragment of ANR1 showed a 170-fold enhancement in transformation efficiency compared to that of the integrative vector PILJ16.

• 에너지공학
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• 제19권1호
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• pp.1-7
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• 2010

본 정책총설은 세계적인 에너지 수급의 불안정과 온실가스 감축에 대한 규제에 대응하여 안정적 경제성장과 환경보호를 달성할 수 있는 대안을 제시하고 있다. 이를 위해 에너지 수급전체를 최적화하는 에너지체인(energy chain) 접근방식을 채택하고 있다. 에너지체인에 걸쳐 저탄소 친환경 에너지기술의 개발 및 산업화, 주력산업의 저탄소 친환경화, 에너지 효율성의 제고, 에너지원의 확보, 그리고 이들 정책간 정책군의 형성 등을 제시하고 있다.