• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 춘계학술발표회
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• pp.36-36
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• 2018

Soybean is the important crop in Asian countries as protein source, oil production and animal feed. Improving soybean using genetic transformation is the principal tool in nowadays. Developing herbicide resistant transgenic soybean plants through Agrobacterium-mediated transformation has been worked in many previous studied. However, the transformation efficiency is still low. Many attempts try to find the optimum media condition for plant regeneration after infection. After transformation, the plant regeneration is very important condition to promote growth of transgenic plant. In this study, we optimized a regeneration condition for two Korean soybean cultivar, Dawonkong and Pungsannamulkong using cotyledon, cotyledonary nodes and hypocotyl as explant. The results showed that shoot regeneration of cotyledonary nodes on B5 medium containing 2 mg/L 6-benzylaminopurine showed the highest percentage of regeneration in Dawonkong (75.8%) while Pungsannamulkong presented high number of shoots 2.12 shoots per explant. For transformation condition, co-cultivation in 7 days showed a high number of GUS positive expression. Most of explants can survived under media including 5 mg/L of glufocinate which refers phosphinotricin for 2-week selection. Washing with 400 mg/L of cefotaxime in several times and selection in plant regeneration media with 400 mg/L of cefotaxime can prevent bacteria growth, effectively.

• 한국물환경학회지
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• 제23권5호
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• pp.712-722
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• 2007

This study screened three natural ores (iron, mangenase, and zinc), two types of slags, and two elemental metals (elemental iron and zinc) to evaluate transformation characteristics of CAH mixtures [e.g. Carbontetrachloride (CT), 1,1,1-Trichloroethane (1,1,1-TCA), and Perchloroethene (PCE)]. To select an effective metal medium to treat the CAH mixtures, we measured transformation capacities (CAH mass ultimately transformed/mass of metal added) and the degree of dechlorination. We also considered economical efficiency of the metal media by comparing the value, CAH mass ultimately transformed divided by the price of metal medium added. A simplified mathematical model adapting CAH transformation capacity, first-order transformation kinetics, and available mass of metal transforming CAH was developed and used for estimating CAH transformation rate coefficient and longevity of the metal medium. CAH transformation capacity for elemental iron and elemental zinc were 4258~7129 and $4215{\sim}6330{\mu}g\;CAH\;transformed/g$ metal added, respectively, which are a factor of 80~200 higher than slags and natural ores. They also showed a factor of 1.1 to 2.2 greater degree of dechlorination than the others. Among natural ores and slags, Zinc ore showed the highest transformation capacity, $47{\sim}53{\mu}g\;CAH\;transformed/g$ metal added. Although zinc ore have smaller transformation capacity than elemental metals, economical efficiency of zinc ore is a factor of 10~20 greater than elemental metals tested. Consequently, zinc ore would be more economical medium than the others tested in this study. We estimated the pseudo first-order transformation rate of zinc ore was in the order of CT > 1,1,1-TCA > PCE.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.I
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• pp.744-747
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• 2005

The exploitable efficiency of UHP is very important in a projection system. We always use lens array for light shaping and energy distribution transformation in conventional projection system. Because of the limitation of arc gap and F/# of elements, the utilize efficiency is always dismal. In this paper we try to bring out a new method to reduce the sparkle formed on the PS converter and enhance the system efficiency.

• 한국빅데이터학회지
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• 제7권2호
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• pp.43-73
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• 2022

디지털트랜스포메이션 추진 목표의 주요 내용중 하나인 생산성 향상 및 비용 절감, 의사결정 효율성 향상 등을 위하여 많은 기업들이 디지털 전환을 위한 다양한 IT도입을 추진중에 있다. 정보기술(IT : Information Technology)은 경쟁력을 결정하는 핵심수단이며, 전 세계적으로 IT도입은 매년 증가하고 있다. 금융산업도 수익창출, 업무 효율성 제고, 전략적 경쟁우위 확보 등을 위해 매년 막대한 IT도입이 활발히 이루어지고 있다. 공공⋅기업부문의 IT도입에 대한 연구는 어느 정도 진행된 것에 비하여 금융산업의 특성을 반영한 실증연구는 미흡한 실정이다. 본 연구에서는 디지털트랜스포메이션 촉진을 위한 금융산업의 IT도입 여부에 영향을 미치는 요인을 도출하고 가중치와 우선순위를 분석하는 것을 목표로 하였다. 금융 IT도입시 요인들의 상대적 우선순위가 각 그룹별로 차이가 존재한다는 점을 데이터 분석을 통하여 밝혀냄으로써 각 그룹별 관점에서 어떤 요인을 우선적으로 고려하여 IT도입을 하여야 하는지에 대한 준거모형으로 활용 할 수 있다는 점에서 그 의의가 있을 것이다.

• Journal of Plant Biotechnology
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• 제3권1호
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• pp.39-44
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• 2001

Plastid transformation was attempted with soybean [Glycine max (L.) Merr.] leaves and photoautotrophic and embryogenic cultures by particle bombardment using the transforming vector pZVII that carries the coding sequences for both subunits of Chlamydomonas reinhardtii Rubisco and a spectinomycin resistance gene (aadA). Spectinomycin resistant calli were selected from the bombarded leaves but the transgene was not present, indicating that the resistance was due to mutations. The Chlamydomonas rbcL and rbcS genes were shown to be site-specifically integrated into the plastid genome of the embryogenic cells with a very low transformation efficiency. None of the transformed embryogenic lines survived the plant regeneration process so no whole plants were recovered. This result does indicate that it should be possible to insert genes into the plastid genome of the important crop soybean if the overall methods are improved.

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.754-758
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• 2008

Agrobacterium tumefaciens-mediated transformation (ATMT) was successfully applied to Monascus ruber. The optimum cocultivation time was 84 h with an efficiency of 900 to 1,000 transformants when $1{\times}10^6$ spores were used with the same volume of bacteria. The stability of transform ants was over 98% after five generations. When M. ruber was transformed with A. tumefaciens YL-63 containing the green fluorescent protein gene (egfp), the green fluorescent signal was observed throughout hyphae, confirming expression of the gene. This efficient transformation and expression system of M. ruber by ATMT will facilitate the study of this fungus at a molecular genetic level.

• 한국측량학회:학술대회논문집
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• 한국측량학회 2004년도 추계학술발표회 논문집
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• pp.9-16
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• 2004

Performing adjustments where the observation equations involve more than a single measurement are General Least Squares(GLS) and Total Least Squares(TLS). This paper introduces theory of the GLS and TLS and compared experimentally accuracy and efficiency of those through 2D conformal coordinate transformation and 2D affine coordinate transformation. In conclusion, in case of 2D coordinate transformation, GLS can produce a little more accurate and efficient than TLS. In survey fields, The GLS and TLS can be used cooperatively for adjusting the actual coordinate measurements.

• 응용통계연구
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• 제33권1호
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• pp.1-10
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• 2020

본 연구에서는 선형회귀모형에서 이상치와 변수변환을 고려한 변수선택 알고리즘을 다룬다. 제안된 방법은 잠재적 이상치를 탐지하여 제거한 후 변수변환 추정을 위해 최소 절사 제곱 추정법을 적용하며 가능한 모든 회귀모형을 비교하여 최종적으로 변수를 선택한다. 정확한 변수 선택과 추정된 모델의 적합도의 맥락에서 방법의 효율성을 보여주기 위해 실제 데이터 분석 및 시뮬레이션 결과가 제시된다.

• 한국육종학회지
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• 제40권2호
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• pp.128-135
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• 2008

Herbicide resistance is the most common trait being tested and thus herbicide?resistant genetically modified plants are now the most widely cultivated worldwide. Here we developed herbicide?resistant transgenic Agrostis mongolica Roshev. by employing an efficient Agrobacterium?mediated transformation procedure with 25.2% of transformation efficiency. The identification and employment of regenerable and reproducible type of callus was one of the most critical factors to ensure success in this study. PCR analysis confirmed that the bar transgene was integrated into the genome of transgenic plants. The expression of 35S?bar gene was confirmed by Northern blot analysis. The transgenic plants showed complete resistance to herbicide, indicating that the bar gene is functional in transgenic plants.

• 한국농촌건축학회논문집
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• 제6권2호
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• pp.65-78
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• 2004

Transformation of housing space is the most accurate physical trail to understand main needs from residents. The scope of the study targeted the 7 si & gun districts in Gyeongsangnam-do, this study is designed to consider the characteristics of space transformation in rural apartment houses and provide basic materials for the development of rural apartment houses. The results of the study are as follow. First, such spaces as corridor and stairway are transformed into storages and outside the houses are installed tap water facility, bicycle storage. and toilet. Second, in autumn seasons when crops and grains are harvested, parking lot and rooftop are used as the space drying such agricultural products. Third, space transformation within the houses has been different depending on family type and main businesses in terms of function and time to seek efficiency and stability of residence. Fourth, rural residents concentrate on a simple change of space usage and replacement of ageing or dilapidated equipment and facilities rather than space transformation incurring more financial burden.