• The Plant Pathology Journal
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• v.25 no.2
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• pp.179-183
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• 2009

To facilitate the genetic manipulation of Cladosporium phlei, a causal agent of leaf spot disease in timothy (Phleum pretense), protoplast-mediated transformation of C. phlei has been developed and the resulting transformants were characterized in this study. Hygromycin B resistance was applied as a dominant selection marker due to the sensitivity of C. phlei to this antibiotic. The transformation efficiency ranged from approximately 20-100 transformants per experiment. Southern blot analysis of stable transformants revealed that transformation occurred by way of stable integration of the vector DNA into the fungal chromosome. PCR analysis and plasmid rescuing of randomly selected transformants suggested that integration of tandem repeat copies of vector DNA was common. In addition, multiple integrations of the transforming vector at different chromosomal sites were also observed. The establishment of a transformation method for C. phlei facilitates strain improvement of this fungus and can be applied as an initial step in the molecular analysis of pigment production in this fungus.

• Journal of KIISE
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• v.42 no.2
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• pp.162-168
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• 2015

Image data is mostly stored in compressed form because of its huge size. Therefore, a series of cumbersome procedures is required to apply a transformation to image data: decompression, extraction of spatial data, transformation and recompression. In this paper, we employ DCT(Discrete Cosine Transform) coefficients to change the spatial presentation of images. DCT is commonly used in still image compression standards such as JPEG and moving picture compression standards such as MPEG-2, MPEG-4, and H.264. In this paper, we derived mathematically the relationship between the geometry transformation in the spatial domain and coefficient changes in the DCT domain and verified it with images in the JPEG file format. Because of the efficiency of transformation in the frequency domain, our findings can be utilized for light-weight partial image encryption for privacy data protection or entertainment contents protection.

• Advanced Industrial SCIence
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• v.2 no.4
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• pp.17-27
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• 2023

The purpose of this study is to improve the efficiency of digital conversion by presenting factors that domestic small and medium-sized manufacturers should consider when attempting digital conversion. Using SME data from 2019 to 2021, we analyze the impact of digital transformation on the financialization of enterprises and which digital technologies affect financialization, and further analyze the mediating effect of green technology innovation between digital transformation and business finance. did The results of the study support the negative impact of digital technologies on corporate finance, and cloud computing technologies have been found to significantly inhibit the level of corporate finance. In addition, it was found that green technology innovation plays a mediating role in suppressing corporate financialization by promoting green technology innovation through digital transformation. Digital transformation influences corporate decision-making through green technology innovation, suggesting that the combination of digital technology and green projects should be considered.

• Journal of the Korea Institute of Building Construction
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• v.24 no.3
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• pp.343-354
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• 2024

This study reviewed the existing legal framework governing construction supervision practices and conducted a survey of expert groups to identify and propose strategies for facilitating the digital transformation of construction supervision processes, with the goal of enhancing the quality and safety of construction projects. The research underscored the necessity for digital transformation to improve quality, safety, and efficiency of construction supervision. Additionally, it highlighted the need for greater consistency and clarity in terminology related to construction supervision. The study also presented recommendations for improvement, which included: establishing a structured supervision checklist to ensure safety and compliance, establishing a specialized construction supervision corporations subject to qualification screening, and introducing evaluation criteria to encourage the adoption of digital transformation technologies in construction supervision. The findings of this study are intended to serve as the foundation for future policy and regulatory frameworks aimed at promoting the digital transformation of construction supervision practices.

• Journal of Korean Home Economics Education Association
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• v.19 no.2
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• pp.103-114
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• 2007

The purpose of this study is to satisfy students in adolescent trends of school uniforms and to provide them with useful material with matching current fashions by analyzing the attitude of students toward uniform transformation in female highschool students and investigating factors influencing on behaviors of uniform transformation. The subjects of this study are 30 students in Daegu with the basic ways of investigating such as depth interview and survey. The results are as follows; First, students agree to wearing school uniforms. As the main reason for wearing school uniforms, students put emphasis on peer pressure. It is also analyzed that the figure of students' body have some influence on the attitude of students toward wearing uniform while students' appearance have no influence on the attitude of students toward wearing uniform. Second, students showed high satisfaction in school uniforms. While students are satisfied with design and color of school uniform, they are not satisfied with practicality, economic efficiency and the quality of textile. Third, the study shows most students have experience of uniform transformation as a result of depth interview about actual condition of uniform transformation. Whereas students usually wear the jacket with its width and length shortened, they tend to lengthen skirt and unstitch pleat of skirt. Fourth, there are many reasons for uniform transformation as a result of depth interview with students. Transformation for satisfying aesthetic desire tops the list, followed by transformation for matching, transformation for peer pressure. As shown by the results above, every school needs to make various efforts to satisfy students, teachers and parents by considering and using students' opinion in order to choose the better school uniforms. In addition, the study related to school uniform transformation need to have more various investigating ways to acquire more authentic results such as expanding the subjects to not only students but also teachers and parents and in-depth comparison between subjects.

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.224-225
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• 2005

• Communications for Statistical Applications and Methods
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• v.7 no.1
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• pp.47-54
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• 2000

This article is concerned with the algorithm for the Chebyshev estimation with/without linear equality and/or inequality constraints. The algorithm employs a linear scaling transformation scheme to reduce the computational burden which is induced when the data set is quite large. The convergence of the proposed algorithm is proved. And the updating and orthogonal decomposition techniques are considered to improve the computational efficiency and numerical stability.

• Proceedings of the Korean Society of Crop Science Conference
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• 1999.05a
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• pp.158-159
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• 1999

• Journal of Plant Biotechnology
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• v.38 no.4
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• pp.251-257
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• 2011

Rice is the most important crop as a model plant for functional genomics of monocotyledons. Rice is usually transformed using Agrobacterium tumefaciens. However, the transformation efficiency using previous method is still low. In this study, we established a new method by modifying the general Agrobacterium protocol especially in the inoculation and co-cultivation step. We directly inoculated Agrobacterium containing a CIPK15 gene under the control of CaMV 35S promoter and NOS terminator in the pCAM1300 vector into the pre-soaked seeds in N6D media for 24 hours. After 7 days of culture at $25^{\circ}C$, calli were formed on seeds cultured on the co-cultivation medium containing an antioxidant compound (1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (3 mg/L silver nitrate). We obtained 35 and 22 transgenic plants in rice cultivars, Gopumbyeo and Ilpumbyeo, with increase of transformation efficiency by 30.4% and 22.6%, respectively compared to the general transformation method. The new method in this study would lead to reduction of substantial labor and time to generate transgenic plants.

• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.235-242
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• 2004

A system for the production of transgenic plants has been developed for Italian ryegrass(Lolium mult리orum Lam.) via Agrobacterium-mediated transformation of embryogenic callus. Mature seed-derived calli were infected and co-cultured with Agrobacterium EHA101 carrying standard binary vector pIG121Hm encoding the hygromycin phosphotransferase(HPT), neomycin phosphotransferase II (NPTII) and intron-oontaining $\beta$g1ucuronidase( intron-GUS) genes in the T-DNA region. The effects of several factors on transformation and the expression of the GUS gene were investigated. Inclusion of 200${\mu}M$ acetosyringone(AS) in inoculation and co-cultivation media lead to a significant increase in stable transformation efficiency. Increasing Agrobacterium cell density up to 1.0 in $OD_{600}$ during infection increased transfonnation efficiency of embryogenic calli. The highest transfonnation efficiency was obtained when embryogenic calli were incoulated with Agrobacterium in the presence of 0.1% Tween20 and 200${\mu}M$ AS. Hygromycin resistant calli were developed into complete plants via somatic embryogenesis. GUS histochemical assay and PCR analysis of transgenic plants demonstrated that transgenes were integrated into the genome of Italian ryegrass.