• 한국약용작물학회:학술대회논문집
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• 2010.10a
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• pp.491-492
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• 2010

• Proceedings of the Botanical Society of Korea Conference
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• 2002.04a
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• pp.112-112
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• 2002

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.234-234
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• 2022

Gene-editing is currently one of the most popular technologies in recent years. Development of the new crop using the gene editing have advantage of improved accuracy and efficiency compared with conventional breeding. Soybean (Glycine max L.) is one of the most important crops worldwide used as food and forage. We tried to establish a system for breeding improvement of soybean through gene-editing technology. For the gene-editing system of soybean, i) selection of efficiency gRNA of targeted gene, ii) efficient genetic transformation of the selected gRNA, iii) selection of trans-clean mutant is essential. First of all, we investigated the selection conditions of gRNA with high editing efficiency of targeted gene using isolated protoplast of soybean. Furthermore, we performed the Agrobacterium-mediated genetic transformation of various soybean cultivars. We identified the tissue culture ability in 23 soybean cultivars for genetic transformation of soybean. The six cultivars with high tissue culture ability were selected and confirmed the transgenic plants in four cultivars. Finally, we established a speed-breeding system as a powerful tool for the fast selection of trans-clean mutants from transgenic plants. Our laboratory will provide the valuable system for improvement of soybean by the gene-editing technology.

• Nuclear Engineering and Technology
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• v.16 no.1
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• pp.29-35
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• 1984

Filtration efficiency tests were conducted on a fiberglass mat filter with DOP aerosol having a diameter from 0.l${\mu}{\textrm}{m}$ to 0.45${\mu}{\textrm}{m}$ in the face velocity range of 1cm/sec to 10cm/sec. Filtration of submicron particles by a fibrous filter is characterized by a face velocity. The size of DOP aerosol which has a minimum removal efficiency decreases with increasing the velocity. A numerical solution of the diffusion equation is obtained for a fiberglass mat filter by using "Kuwabara's cell model" for the flow field and Von Mises Transformation for the actual flow around a fiberglass. The present theoretical results agree quite well with the experimentals for fiberglass mat. This result could be contributed to predict the removal efficiency on an air filter and to optimize the operating condition of an air purification system with a filter. a filter.

• Journal of Plant Biotechnology
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• v.50
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• pp.56-62
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• 2023

Transgenic lilies have been obtained using Agrobacterium tumefaciens (AGL1) with the plant scale explants, followed by DL-phosphinothricin (PPT) selection. In this study, scales of lily plants cv. "red flame" were transformed with the pCAMBIA3301 vector containing the gus gene as a reporter and the blpR gene as a selectable marker, as well as a gene of interest showing herbicide tolerance, both driven by the CaMV 35S promoter. Using a 20-minute infection time and a 5-day cultivation period, factors that optimized and demonstrated a high transformation efficiency were achieved. With these conditions, approximately 22-27% efficiency was observed for Agrobacterium-mediated transformation in lilies. After transformation with Agrobacterium, scales of lilies were transferred to MS medium without selective agents for 2 weeks. They were then placed on selection MS medium containing 5 mg/L PPT for a month of further selection and then cultured for another 4-8 weeks with a 4-week subculture regime on the same selection medium. PPT-resistant scales with shoots were successfully rooted and regenerated into plantlets after transferring into hormone-free MS medium. Also, most survived putatively transformed plantlets indicated the presence of the blpR gene by PCR analysis and showed a blue color indicating expression of the gus gene. In conclusion, when 100 scales of lily cv. "red flame" are transformed with Agrobacterium, approximately 22-27 transgenic plantlets can be produced following an optimized protocol. Therefore, this protocol can contribute to the lily breeding program in the future.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.11a
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• pp.809-814
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• 2006

In this paper, the modified direct method employing beam transformation technique is proposed in order to efficiently calculate hydroelastic responses of floating structure. Since the proposed method expresses the displacements of three-dimensional structure with those of transformed beam which leads to small number of equations of motion, the method is numerically efficient compared to the conventional direct method. To verify the efficiency of the proposed method a 500 m-long floating structure under wave loads is considered in numerical example. Displacements, bending moments, torsion moments and shear forces are calculated and computing tine is examined. The results are also compared with those of the conventional direct method.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.1
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• pp.70-78
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• 2004

This paper presents study of efficiency of wavelet transformation for on-line chatter detection during hard fuming process. From comparison with other time series and statistical methods such as fast fourier transformation (FFT), Kurtosis and standard deviation (STD), wavelet transform is better than others in on-line chatter detection. With using wavelet function with pseudo frequency corresponding to chatter frequency, chatter could be detected more sensitively. And for both force signal from dynamometer and displacement signal from capacitance type cylindrical sensor (CCS), wavelet transform with DB2 function on level 4 could be well used for chatter detection in hard turning process.

• Bulletin of the Korean Mathematical Society
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• v.51 no.2
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• pp.595-612
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• 2014

The spectral collocation method for a second order elliptic boundary value problem on a domain ${\Omega}$ with curved boundaries is studied using the Gordon and Hall transformation which enables us to have a transformed elliptic problem and a square domain S = [0, h] ${\times}$ [0, h], h > 0. The preconditioned system of the spectral collocation approximation based on Legendre-Gauss-Lobatto points by the matrix based on piecewise bilinear finite element discretizations is shown to have the high order accuracy of convergence and the efficiency of the finite element preconditioner.

• 한국전산유체공학회:학술대회논문집
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• 2011.05a
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• pp.261-265
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• 2011

An adaptive wavelet transformation method with high order accuracy is proposed to allow efficient and accurate flow computations. While maintaining the original numerical accuracy of a conventional solver, the scheme offers efficient numerical procedure by using only adapted dataset. The main algorithm includes 3rd order wavelet decomposition and thresholding procedure. After the wavelet transformation, 3rd order of spatial and temporal accurate high order interpolation schemes are executed only at the points of the adapted dataset. For the other points, high order of interpolation method is utilized for residual evaluation. This high order interpolation scheme with high order adaptive wavelet transformation was applied to unsteady Euler flow computations. Through these processes, both computational efficiency and numerical accuracy are validated even in case of high order accurate unsteady flow computations.

• Proceedings of the Materials Research Society of Korea Conference
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• 2010.05a
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• pp.39.2-39.2
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• 2010

Recently, hot stamping process has been paid attention greatly by automobile makers in accordance with the fuel efficiency and environmental issues as well as crash safety issue. The hot-stamped parts, however, demand extreme mechanical properties such as tensile strength of over 1470 MPa or equivalently Vickers hardness of around 450. In this work, to meet the demand efficiently, a method to predict mechanical property of hot-stamped parts based on numerical phase transformation scheme has been proposed associated with the thermo-mechanical coupled finite element analysis. This work deals with various phase transformation equations and validates them to select appropriate model for 0.2C-0.1Si-1.4Mn-0.5Cr-0.01Mo-0.002B steel sheet. The authors show that an efficient method saving time and cost to develop hot-stamped automobile parts ensuring suitable mechanical properties such as Vickers hardness and strength.