• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.05a
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• pp.954-957
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• 2015

A recombinant baculovirus vector systems were composed of genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD). These recombinant baculovirus vector system were transfected into various cell lines and tissues and confirmed gene transfer and expression of these vector systems with only control vector system. From the result, gene transfer and gene expression of recombinant baculovirus vector systems were superior in terms of efficacy and safety than in the control vector system.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.94-94
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• 2002

Lactadherin (formerly known as BA46), a major glycoprotein of the human milk fat globule membrane, is abundant in human breast milk and breast carcinomas and may prevent symptomatic rotavirus infections. In this study, under the control of mouse whey acidic protein (WAP) promoter, the expression pattern of lactadherin (Ltd) in lactogenic hormone-dependent mouse mammary epithelial cell line HC11 were tested. pLNWLtd construct containing 2.4 kilobases of the WAP promoter and 1.5 kilobases of human lactadherin gene was stably transfered into HC11 cells using retroviral vector system. Integration and expression level of the transgene was estimated using PCR and RT-PCR, respectively. Prominent induction of Ltd gene under the WAS promoter was accomplished in the presence of insulin, hydrocortisone and prolactin, while induction with insulin alone resulted in lower expression. Our results demonstrate that the expression of the transgene is increased by synergistic effect of several lactogenic hormones, including insulin, hydrocortisone, and prolactin.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.10a
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• pp.838-841
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• 2015

Recombinant baculovirus vector is composed of genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD). This recombinant baculovirus vector was transfected into cell lines and tissues and then found out a novel possibility in view of gene transfer and gene expression in comparison to other vector systems. Efficacy of gene transfer and gene expression of this recombinant baculovirus vector was higher than any other vector system.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.25 no.11
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• pp.1659-1666
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• 2001

The objective of this study was to investigate the characteristics of air flow and heat transfer caused by trapezoid rods array in impinging air jet system. Trapezoid rods have been set up on front of flat plate to act as a turbulence promoter. Local Nusselt numbers were determined as a function of three parameters : (a) the space from re(Is to heating surface(C=1, 2, 4mm), (b) the pitch between each rods(P=30, 40, 50mm), (c) the distance from nozzle exit to flat plate(H/B=2, 6, 10). The measurements were compared with those of the experiment without trapezoid rods. As a result, when rods are installed in front of the impinging palate, the acceleration of the flow and the eddies due to the rods seem to contribute to the heat transfer enhancement. Heat transfer performance was best under the condition of C=1mm and as the pitch is 30mm. The maximum rate of heat transfer augmentation is about 1.9 times greater compared to that without trapezoid rods.

• Journal of Ceramic Processing Research
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• v.20 no.1
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• pp.18-23
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• 2019

Chemical looping combustion (CLC) is a promising carbon capture and storage (CCS) technology whose efficiency and cost primarily relies on the oxygen carrier materials used. In this paper, gadolinium-doped ceria (GDC, Ce0.9Gd0.1O1.95) was added as a promoter to improve the oxygen transfer rate of MgMnO3-δ oxygen carrier materials. Increasing GDC content significantly increased the oxygen transfer rate of MgMnO3-δ-GDC composites for the reduction reaction due to an increase in the surface adsorption of CH4 via oxygen vacancies formed on the surface of the GDC. On the other hand, the oxygen transfer rate for the oxidation reaction decreased linearly with increasing GDC content due to the oxygen storage ability of GDC. Adsorbed oxygen molecules preferentially insert themselves into oxygen vacancies of the GDC lattice rather than reacting with (Mg,Mn)O to form MgMnO3-δ during the oxidation reaction.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.356-363
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• 2015

In order to study genetic engineering in trees, the characterization of genes and promoters from trees is necessary. We isolated the promoter region (867 bp) of Pagns-LTP from poplar (P. alba ${\times}$ P. glandulosa) and characterized its activity in transgenic poplar plants using a ${\beta}$-glucuronidase (GUS) reporter gene. High-level expression of the Pagns-LTP transcript was found in poplar roots, while comparatively low-level expression was found in the young leaves. Pagns-LTP mRNA was not detected in other poplar tissues. Additionally, transgenic poplar plants that contained a Pagns-LTP promoter fused to a GUS reporter gene, displayed tissue-specific GUS enzyme activity localized in root tissue. In silico analysis of the Pagns-LTP promoter sequence reveals the presence of several cis-regulatory elements responsive to phytohormones, biotic and abiotic stresses, as well as those regulating tissue-specific expression. These results demonstrate that the Pagns-LTP promoter has tissue-specific expression activity in poplar roots and leaves that may be involved in organ development and plant resistance to various stresses. Therefore, we anticipate that the Pagns-LTP promoter would be a useful tool to genetically optimize woody plants for functional genomics.

• Proceedings of the KSME Conference
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• 2001.11b
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• pp.260-267
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• 2001

The objective of this study was to investigate the characteristics of jet flow and heat transfer caused by trapezoid rods array in impinging jet system. In this study, trapezoid rods have been set up in front of flat plate to serve as a turbulence promoter. The bottom width of trapezoid rod was W=4, 8mm and oblique angle were $80^{\circ}$. The space from rods to the heating surface was C=1, 2, 4mm, the pitch between each rods was P=30, 40, 50mm, and the distance from nozzle exit to flat plate was H=100, 500mm. This results were compared with the case without trapezoid rods. As a result, when rods are installed in front of the impinging plate, the acceleration of the jet flow and the eddies due to the rods seem to contribute to the heat transfer enhancement. Among test conditions, the heat transfer performance was best for the condition of W=8mm, C=1mm, P=30mm and H/B=10. The maximum heat transfer rate is about 1.9 times larger than that without trapezoid rods.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.13 no.9
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• pp.904-913
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• 2001

The objective of this study was to investigate the characteristics of jet flow and heat transfer caused by trapezoid rods array in impinging jet system. In this study, trapezoid rods have been set up in front of flat plate to serve as a turbulence promoter. The bottom width of trapezoid rod was W=4, 8 mm and oblique angle were 80$^{\circ}$. The space from rods to the heating surface was C=1, 2, 4 mm, the pitch between each rods was P=30, 40, 50 mm, and the distance from nozzle exit to flat plate was H=100, 500 mm. This results were compared with the case without trapezoid rods. As a result, when rods are installed in front of the impinging plate, the acceleration of the jet flow and the eddies due to the rods seem to contribute to the heat transfer enhancement. Among test conditions, the heat transfer performance was best for the condition of W=8 mm, C=1 mm, P=30 mm and H/B=10. The maximum heat transfer rate is about 1.9 times larger than that without trapezoid rods.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.71-71
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• 2002

Promoters for milk proteins have been used far producing transgenic animals due to their temporal and spatial expression patterns. ${\beta}$-casein, a calcium-sensitive casein, is a major milk protein that corresponds ca. 30 per cent of total milk protein. Expression of ${\beta}$-casein is controlled by lactogenic hormones such as prolactin (PRL), composite response elements (CoREs) and transcription factors. CoREs are clusters of transcription factor binding sites containing both positive and negative regulatory elements. ${\beta}$-casein gene promoter contains various regions (CoREs) for gene transcription. We analyzed the promoter region by mutagenesis using exonuclease III and linker-scanning. Transcription control elements usually are positioned in 5'-flanking region of the gene. However, in some cases, these elements are located in other regions such as intron 1. The nucleotide sequences of ${\beta}$-casein promote. region has been reported (E12614). However, the properties of the promoter is not yet clear. In this study, we plan to investigate the properties of cis-regulating elements of porcine ${\beta}$-casein by mutation analysis and expression analysis using dual-luciferase repoter assay system.

• Membrane and Water Treatment
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• v.9 no.2
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• pp.115-121
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• 2018

Artificial neural network (ANN) simulation is used to predict the dynamic change of permeate flux during wheat starch industry wastewater microfiltration with and without static turbulence promoter. The experimental program spans range of a sedimentation times from 2 to 4 h, for feed flow rates 50 to 150 L/h, at transmembrane pressures covering the range of $1{\times}10^5$ to $3{\times}10^5Pa$. ANN predictions of the wastewater microfiltration are compared with experimental results obtained using two different set of microfiltration experiments, with and without static turbulence promoter. The effects of the training algorithm, neural network architectures on the ANN performance are discussed. For the most of the cases considered, the ANN proved to be an adequate interpolation tool, where an excellent prediction was obtained using automated Bayesian regularization as training algorithm. The optimal ANN architecture was determined as 4-10-1 with hyperbolic tangent sigmoid transfer function transfer function for hidden and output layers. The error distributions of data revealed that experimental results are in very good agreement with computed ones with only 2% data points had absolute relative error greater than 20% for the microfiltration without static turbulence promoter whereas for the microfiltration with static turbulence promoter it was 1%. The contribution of filtration time variable to flux values provided by ANNs was determined in an important level at the range of 52-66% due to increased membrane fouling by the time. In the case of microfiltration with static turbulence promoter, relative importance of transmembrane pressure and feed flow rate increased for about 30%.