• Journal of Applied Biological Chemistry
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• v.51 no.3
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• pp.102-110
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• 2008

Complementation assay of the urea uptake-defective yeast mutants led to the identification of the Arabidopsis AtTIP4;1 gene encoding the aquaporin. However, its physiological functions still remain elusive. In the present study, histochemical and genetic analyses were performed to understand the physiological roles of AtTIP4;1 in urea uptake. The AtTIP4;1 product was detectible in the roots, but not in the leaves, the stem, and the flower. Its promoter allowed the expression of the $\beta$-glucuronidase reporter gene in the roots and the apical meristem in Arabidopsis. The AtTIP4;1 products were induced under nitrogen-deficient conditions. To investigate the role of the tonoplast intrinsic protein in urea transport and developments, Arabidopsis with the loss- and the gain-of-function mutations by T-DNA insertion in AtTIP4;1 and 35S promoter-mediated overexpression of AtTIP4;1 were identified, respectively. The transfer DNA insertion and the AtTIP4;1-overexpressed plants showed normal growth and development under normal or abiotic stress growth conditions. The urea-uptake studies using $^{14}C$-labeled urea revealed higher accumulation of urea in the AtTIP4;1-overexpressed plants. These results provide evidence that overexpression of AtTIP4;1 leads to the increase in the urea-uptake rate in plants without detectable defects to the growth and development.

• Proceedings of the Korea Society for Energy Engineering kosee Conference
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• 2002.05a
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• pp.227-234
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• 2002

With the increasing environmental consideration and stricter regulations, gasification of waste is considered to be more attractive technology than conventional incineration for energy recovery as well as material recycling. The experiment for combustible waste mixed with plastic and cellulosic materials was performed in the fixed bed gasifier to investigate the gasification behavior with the operating conditions. Waste pelletized with a diameter of 2~3cm and 5cm of length was gasified at the temperature range of 1100~145$0^{\circ}C$. It was shown that the composition of H$_2$ was in the range of 30~40% and CO 15~30% depending upon oxygen/waste ratio. Casification of waste due to thermoplastic property from mixed plastic melting and thermal cracking shows a prominent difference from that of coal or coke. It was desirable to maintain the top temperature up to foot to ensure the mass transfer and uniform reaction through the packed bed. As the bed height was increased, the formation of H$_2$ and CO was increased whilst $CO_2$ decreased by the char-$CO_2$ reaction and plastic cracking. From the experimental results, the cold gas efficiency was around 61% and heating values of product gases were in the range of 2800~3200㎉/Nm3.

• Korean Journal of Food Science and Technology
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• v.18 no.5
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• pp.351-356
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• 1986

The effects of drum spacing, steam pressure and drum speed on drying rate of minced fish flesh on both single and double drum dryers were studied. Starch additions in the form of tapioca flour up to 2.5% have been found satisfactory for aiding in sheet formation at the doctor blade. When the retention time was adjusted to maintain a constant product moisture, the highest production rate was obtained at the smallest drum spacing and the highest steam pressure within the limits of experimental conditions considered. The operating conditions suitable for producing the flakes with 5% moisture were: 100 kPa (steam pressure), 0.1 mm (drum spacing) and 3 rpm (drum speed). The production rate and overall heat transfer coefficient under these conditions were $12.1\;kg/m^2$hr and 950 $W\;/m^2K$ respectively. The drying data were fitted well to the conventional drying model, namely $MR\;=\;A\;\exp\;(-k{\theta})$, resulting in the various drying constants depending the operating conditions.

• Journal of Environmental Science International
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• v.4 no.3
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• pp.107-107
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• 1995

This study was conducted to evaluate the effects of pressure and dissolved oxygen concentration on the activated slut비e and to determine the optimum depth of deep shaft process. Some results from this study were summarized as follows. 1. It is considered that low sludge product in the activated sludge system maintaining high dissolved oxygen concentration is attributed to the increase of endogeneous respiration rate caused by the increase of aerobic zone in the sludge floe. 2. The increase of dissolved oxygen concentration does not affect to the increase of organic removal efficiency greatly and therefore the limiting factor is the substrate transfer into the inner part of floe. 3. The yield coefficient, Y is decreased in proportion to the increase of oxygen concentration. In this study, Y values arre ranged from 0.70 to 0.41 according to the variation of dissolved oxygen concentration from 18.0mg/$\ell$ to 258 mg/$\ell$. 4. The optimum depth of deep shaft process should be determined within the limits of non-toxicity to the microorganism and it is about loom in this study.

• Rapid Communication in Photoscience
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• v.3 no.4
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• pp.70-72
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• 2014

Visible-light irradiation of MeCN solution containing di(hydroxo)metallo(tetraphenyl)porphyrin complex $(tppM(OH)_2$: 1a; $M=Sb(V)^+Br^-$, 1b; $M=P(V)^+Cl^-$, 1c; M=Ge(IV)) and 2-mercaptoethanol (2-ME) as a substrate under aerated condition gave bis(2-hydroxyethyl)disulfide (2-HEDS) as an oxidative product of 2-ME. It is indicated that the oxidation of 2-ME should proceed with a photocatalytic process by 1, because the turn over number (TON) for the formation of 2-HEDS was over unit. The TON was determined to be 642 as a maximum value when 1a was used as a sensitizer. The formation of 2-HDES was extremely slow under argon atmosphere. The fluorescence of 1 was not quenched by 2-ME at all, and the free energy change (${\Delta}G$) with electron transfer (ET) from 2-ME to excited triplet state of $1(^31^*)$ was estimated as a negative value. The quenching rate constant ($k_r$) of $^31^*$ by 2-ME, obtained by the kinetics for the formation of 2-HEDS, strongly depends on ${\Delta}G$. These findings indicate that 1-sensitized oxidation was initiated by photoinduced ET from 2-ME to $^31^*$ to generate both radical cation of 2-ME ($2-ME^{+\bulle}$) and porphyrin radical anion ($1^{-\bulle}$), resulting that the formation of 2-HEDS can be proceeded by the dimerization of $2-ME^{+\bulle}$, and through a catalytic cycle due to returning to 1 by the ET from $1^{-\bulle}$ to molecular oxygen.

• Journal of the Korean Society of Clothing and Textiles
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• v.30 no.6 s.154
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• pp.851-858
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• 2006

The human have constantly pursued the beauty through the history. Especially, the pursuit for the beauty of the human's body means that the subject is at one with the object pursued by Ire subject, which so called as the most active behavior of searching far the beauty. However it sometimes shows that human have tried excessively to search for the beauty of the human's ideal body in the history, and to the degree of hurting the body. For example, the corset was eagerly worn by women with the hope of a satisfied silhouette because the slender waist and the busty bosom were demanded as the woman's ideal body at 18C and 19C(the age of Victorian). The purpose of this study was to investigate the corset fashion in 18C, 19C, one of the most aggressive fashions out of the unreasonable fads, using narcissism that Freud mentioned as inside energy called libido. And the reason why to use the theory of narcissism in this study was that fashion as social outcome is thought to be influenced by the environment but the wearing behavior is considered totally private, it is particularly when focused on the excess ive energy, and the scope was limited to the investigation into the source of the energy binding the own body to hurting. The analysis about this craze of a corset with the view of the theory of narcissism as follows. First, women could mold the ideal concretely in surroundings that ideal woman was constituted just to focus on the appearance, moreover, the mother reinforced the narcissism of their offsprings. Also, the society demanded women to be chaste, and did not allowed for women to reveal libido to the external. As a result of this, libido should be ended up concentrating on ego. With this process, it to be difficult for 'ideal ego' impossible for attainment to transfer to compromising product, ego ideal. That is, women did not think entirely to be out of the question to reach the ideal ego, which meant the energy to tight waists was given women.

• Journal of Microbiology and Biotechnology
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• v.21 no.2
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• pp.204-211
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• 2011

To yield high concentrations of protein expressed by genetically modified Escherichia coli, it is important that the bacterial strains are cultivated to high cell density in industrial bioprocesses. Since the expressed target protein is mostly accumulated inside the E. coli cells, the cellular product formation can be directly correlated to the bacterial biomass concentration. The typical way to determine this concentration is to sample offline. Such manual sampling, however, wastes time and is not efficient for acquiring direct feedback to control a fedbatch fermentation. An E. coli K12-derived strain was cultivated to high cell density in a pressurized stirred bioreactor on a pilot scale, by detecting biomass concentration online using a capacitance probe. This E. coli strain was grown in pure minimal medium using two carbon sources (glucose and glycerol). By applying exponential feeding profiles corresponding to a constant specific growth rate, the E. coli culture grew under carbon-limited conditions to minimize overflow metabolites. A high linearity was found between capacitance and biomass concentration, whereby up to 85 g/L dry cell weight was measured. To validate the viability of the culture, the oxygen transfer rate (OTR) was determined online, yielding maximum values of 0.69 mol/l/h and 0.98mol/l/h by using glucose and glycerol as carbon sources, respectively. Consequently, online monitoring of biomass using a capacitance probe provides direct and fast information about the viable E. coli biomass generated under aerobic fermentation conditions at elevated headspace pressures.

• Journal of the Korean association of regional geographers
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• v.18 no.4
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• pp.400-413
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• 2012

The purpose of this study was to look into the formation process and characteristics of local high-tech industrial complex with an example of the Ochang Scientific Industrial Complex and analyzed the characteristics by business type and size, transfer process of the companies in the Complex. For this purpose, statistical data of the companies were analyzed and a survey of them was conducted, and the following results were obtained. First, the Ochang Scientific Industrial Complex was created for agglomeration of IT industries in early days but now there are IT industries and BT industries mixed due to internal and external economic condition. Second, there are more small and medium companies than large companies in the Ochang Scientific Industrial Complex. These companies are in the stage of developing new products and marketing, and many of them were parts and materials developers of core technology or high innovation with venture identification. Third, high rates of the companies in the Ochang Scientific Industrial Complex came from the capital area and the Chungcheong area and the companies which moved in the Complex with their head quarters accounted for more than 70 percent. Fourth, the companies were classified into 5 types according to the type of product and the level of technical innovation.

• Archives of design research
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• v.14 no.4
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• pp.5-14
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• 2001

Among Human Sensibility-oriented products a gap between the images that designers try to express through that product and users emotional evaluation becomes an issue. The data on the correlation between image words used for design evaluation and images used in the design process are especially significant. This study based on these correlations suggests a Fuzzy retrieval system supporting styling design with images and image words. In the system, the relational data are demonstrated by Fuzzy thesaurus as correlation coefficient from the degree of similarity among image words. And the degree of similarity is produced based on image evaluation. Image retrieval is conducted by the algorithm of Fuzzy thesaurus development, 1) among image words, 2) images to image words, 3) image words to images and 4) among images: 4 different modes are provided as retrieval modes. Also transfer between modes is carried by direct operating interface, therefore divergent thinking and convergent thinking is supported well. The system consists of operation for the gap and the measurement unit of emotional evaluation, and visualization units. Under unified interface environments are set in order for consistency of the operation.

• Journal of Pharmaceutical Investigation
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• v.32 no.3
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• pp.153-159
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• 2002

Gene therapy is fundamentally a sophisticated drug delivery technology to cure a disease by the transfer of genetic material to modify living cells. In other words, the gene is used as a therapeutic drug much like a chemical compound is employed in chemotherapy. Currently almost 600 clinical trials are underway worldwide since the first clinical trials carried out in 1990 to treat adenosine deaminase deficiency using retroviral vectors. Despite the great progress still is there no gene therapy product being approved as a new drug. This is partly due to a lack of an ideal gene delivery system that is safe and can provide stable, optimal level production of the therapeutic proteins in the cell. This review covers the current status of several different biological and physico-chemical agents that are being developed as gene delivery vehicles. Although gene therapy promises great hopes toward the cure of a broad spectrum of genetic and acquired diseases, the success of gene therapy heavily asks for the development of vector systems for safe and efficient application in humans.