• Korean Journal of Plant Resources
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• v.23 no.6
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• pp.526-534
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• 2010

Genomes of clusters of related eukaryotes are now being sequenced at an increasing rate. In this paper, we developed an accurate, low-cost method for annotation of gene prediction and exon-intron structure. The gene prediction was adapted for delta 1-pyrroline-5-carboxylate-synthetase (p5cs) gene from China wild-type of the halophytic Leymus chinensis (Trin.), naturally adapted to highly-alkali soils. Due to complex adaptive mechanisms in halophytes, more attentions are being paid on the regulatory elements of stress adaptation in halophytes. P5CS encodes delta 1-pyrroline-5-carboxylate-synthetase, a key regulatory enzyme involved in the biosynthesis of proline, that has direct correlation with proline accumulation in vivo and positive relationship with stress tolerance. Using analysis of reverse transcription-polymerase chain reaction (RT-PCR) and PCR, and direct sequencing, 1076 base pairs (bp) of cDNA in length and 2396 bp of genomic DNA in length were obtained from direct sequencing results. Through gene prediction and exon-intron structure verification, the full-length of cDNA sequence was divided into eight parts, with seven parts of intron insertion. The average lengths of determinated coding regions and non-coding regions were 154.17 bp and 188.57 bp, respectively. Nearly all splice sites displayed GT as the donor sites at the 5' end of intron region, and 71.43% displayed AG as the acceptor sites at the 3' end of intron region. We conclude that this method is a cost-effective way for obtaining an experimentally verified genome annotation.

• BMB Reports
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• v.40 no.6
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• pp.1083-1089
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• 2007

The HAP complex has been found in many eukaryotic organisms. HAP recognizes the CCAAT box present in the promoters of 30% of all eukaryotic genes. The HAP complex consists of three subunits - HAP2, HAP3 and HAP5. In this paper, we report the biological function of the AtHAP3b gene that encodes one of the HAP3 subunits in Arabidopsis. Compared with wild-type plants, hap3b-1 and hap3b-2 mutants exhibited a delayed flowering time under long-day photoperiod conditions. Moreover, the transcription levels of FT were substantially lower in the mutants than in the wild-type plants. These results imply that AtHAP3b may function in the control of flowering time by regulating the expression of FT in Arabidopsis. In a subsequent study, AtHAP3b was found to be induced by osmotic stress. Under osmotic stress conditions, the hap3b- 1 and hap3b-2 mutants flowered considerably later than the wild-type plants. These results suggest that the AtHAP3b gene plays more important roles in the control of flowering under osmotic stress in Arabidopsis.

• Journal of Internet Computing and Services
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• v.21 no.1
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• pp.211-219
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• 2020

One of the major advancements of the Fourth Industrial Revolution is the use of Internet of Drones (IoD), which combines the Internet of Things (IoT) and drone technology. IoD technology is especially important for efficiently and economically operating C4ISR operations in actual battlefields supporting various combat situations. The purpose of this study is to solve the problems of limited battery capacity of drones and lack of budgeting criteria for military drone transcription, introduction, and operation. If the mission area is defined and corresponding multi-drone hovering check points and mission completion time limits are set, then an energy and time co-optimized scheduling and operation control scheme is needed. Because such a scheme does not exist, in this paper, a Drone Force Deployment Optimization (DFDO) scheme is proposed to help schedule multi-drone operation scheduling and networked based remote multi-drone control.

• The Journal of the Acoustical Society of Korea
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• v.16 no.5
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• pp.50-59
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• 1997

In this paper, we implement an automatic speech segmentation and labeling system which marks phone boundaries automatically for constructing the Korean speech database. We specify and implement the system based on conventional speech segmentation and labeling techniques, and also develop the graphic user interface(GUI) on Hangul $Motif^{TM}$ environment for the users to examine the automatic alignment boundaries and to refine them easily. The developed system is applied to 16kHz sampled speech, and the labeling unit is composed of 46 phoneme-like units(PLUs) and silence. The system uses both of the phonetic and orthographic transcription as input methods of linguistic information. For pattern-matching method, hidden Markov models(HMM) is employed. Each phoneme model is trained using the manually segmented 445 phonetically balanced word (PBW) database. In order to evaluate the performance of the system, we test it using another database consisting of sentence-type speech. According to our experiment, 74.7% of phoneme boundaries are within 20ms of the true boundary and 92.8% are within 40ms.

• The Plant Pathology Journal
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• v.21 no.3
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• pp.258-261
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• 2005

We conducted a survey on pepper virus diseases in 31 regions in Korea from November 2001 to December 2004. Using electron microscopy, test plant reaction, rapid immuno-filter paper assay (RIPA), reverse transcription-polymerase chain reaction (RT-PCR) and/or analysis of viral nucleotide sequences, we found a number of viruses from 1,056 samples that we collected. These included Cucumber mosaic virus (CMV), Pepper mottle virus (PepMoV), Pepper mild mottle virus (PMMoV), Broad bean wilt virus 2 (BBWV2), Tobacco mild green mosaic virus (TMGMV), and Tomato spotted wilt virus (TSWV). Of the samples analyzed, $343(32.5\%)$ were infected with CMV, $209(19.8\%)$ with PepMoV, $141(13.4\%)$ with PMMoV, $12(1.1\%)$ with BBWV2, $40(3.8\%)$ with TMGMV, $5(0.5\%)$ with TSWV, $153(14.5\%)$ with CMV and PepMoV, $54 (5.1\%)$ with CMV and PMMoV, $31(2.9\%)$ with PepMoV and PMMoV, $3(0.3\%)$ with CMV and BBWV2, $1(0.1\%)$ with CMV, PepMoV and BBWV2, $8(0.8\%)$ with CMV, PepMoV and PMMoV, and $30 (2.8\%)$ samples were infected with viruses which were not identified. CMV was the most predominant virus in all inspected fields and the number of the samples infected with PMMoV was relatively low as compared PepMoV infection level in pepper. TMGMV was only found in the southern part of Korea, while TSWV was isolated in Anyang and Yesan. However, we did not encounter in this survey the Alfalfa mosaic virus (AMV), Potato virus Y (PVY), Tobacco mosaic virus (TMV), and Pepper vein chlorosis virus (PVCV).

• Proceedings of the KSPS conference
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• 1996.10a
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• pp.456-463
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• 1996

In this paper, we discussed three cases to see the effects of the characteristics of Hangul writing system. In applications such as computer Hangul shorthands for ordinary people and pushbuttons with Hangul characters engraved, we found that there is much advantage in using Hangul. In case of Hangul Transliteration, we discussed some problems which are related with the characteristics of Hangul writing system. Shorthands use 3-set keyboards in England, America, and Korea. We saw how ordinary people can do computer Hangul shorthands, whereas only experts can do computer shorthands in other countries. Specifically, the facts that 1) Hangul characters are grouped into syllables (syllabic blocks) and that 2) there is already a 3-set Hangul keyboard for ordinary people allow ordinary people to do computer Hangul shorthands without taking special training as with English shorthands. This study was done by the author under the codename of 'Sejong 89'. In contrast like QWERTY or DVORAK, a 2-set Hangul keyboard cannot be used for shorthands. In case of English pushbuttons, one digit is associated with only one character. However, by engraving only syllable-initial characters on the phone pushbuttons, we can associate one Hangul "syllable" with one digit. Therefore, for a given number of digits, we can associate longer words or more meaningful words in Hangul than in English. We discussed the problems of the Hangul Transliteration system proposed by South Korea and suggested their solutions, if available. 1) We are incorrectly using the framework of transcription for transliteration. To solve the problem, the author suggests that a) we include all complex characters in the transliteration table, and that b) we specify syllable-initial and -final characters separately in the table. 2) The proposed system cannot represent independent characters and incomplete syllables. 3) The proposed system cannot distinguish between syllable-initial and -final characters.

• BMB Reports
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• v.39 no.6
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• pp.686-695
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• 2006

Interleukin-2 enhancer binding factor 2 (ILF2) was reported to regulate transcription of interleukin-2 (IL-2), a central cytokine in the regulation of T-cell responses. This property of ILF2 was well characterized in human and mammals, but little is known in bony fish. In this paper, an ILF2 homologue was cloned and well characterized from Tetraodon nigrovirid is for the further investigation of the function of ILF2 in bony fish. The full-length Tetraodon ILF2 cDNA was 1380 bp in size and contained an open reading frame (ORF) of 1164 bp that translates into a 387 amino-acid peptide with a molecular weight of 42.9 kDa, a 5' untranslated region (UTR) of 57 bp, and a 3' UTR of 159 bp containing a poly A tail. The deduced peptide of Tetraodon ILF2 shared an overall identity of 58%~93% with other known ILF2 sequences, and contained two N-glycosylation sites, two N-myristoylation sites, one RGD cell attachment sequence, six protein kinase C phosphorylation sites, one amino-terminal RGG-rich single-stranded RNA-binding domain, and a DZF zinc-finger nucleic acid binding domain, most of which were highly conserved through species compared. Constitutive expression of Tetraodon ILF2 was observed in all tissues examined, including gill, gut, head kidney, spleen, liver, brain and heart. The highest expression was detected in heart, followed by liver, head kidney and brain. Stimulation with LPS did not significantly alter the expression of Tetraodon ILF2. Gene organization analysis showed that the Tetraodon ILF2 gene have fifteen exons, one more than other known ILF2 genes in human and mouse. Genes up- and down-stream from the Tetraodon ILF2 were Rpa12, Peroxin-11b, Smad4, Snapap and Txnip homologue, which were different from that in human and mouse.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.35 no.12
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• pp.1309-1316
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• 2011

This paper presents a high-speed RNA microextractor for the direct isolation of RNA from blood lysate using magnetic oligo(dT) beads. The extraction is performed through lateral magnetophoresis, which is induced by a ferromagnetic wire array inlaid. With this RNA microextractor, more than 80% of the magnetic beads could be separated at a flow rate up to 20 ml/h, and the overall extraction procedure was completed within 1 min. The absorbance ratio of RNA to protein(A260/A280) was greater than 1.7, indicating that the extraction technique yields pure RNA. The feasibility of using this technique in reverse transcription polymerase chain reaction procedures was investigated by cDNA synthesis and PCR processes. The results confirmed that the RNA microextractor is a practical device for easy, fast, and high-precision RT-PCR using minimal amounts of reagent.

• Journal of Conservation Science
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• v.25 no.2
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• pp.171-178
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• 2009

When we considered the superior ability of the semi-water soluble urethane 1st transcription and final epoxy products, the stability for moving historic sites depend on physical properties and the peel off state of separation media. In this paper, we synthesised three type of water-borne epoxy solution without using a surfactant, and investigated the peel off state, physical properties, and the state between urethane surface and epoxy surface after exfoliation. The life time of water-borne separation media is over the 60 days. When it is used the 30% solution of water-borne separation media, it made good separation of urethane pre-products surface and epoxy final product surface and no color change. The separation tension pressure is about 15~50 kg/$cm^2$ and there is no surface whitewash phenomenon. We suggest that this water-borne epoxy will be best material as separation media of low viscosity type.

• The Journal of the Acoustical Society of Korea
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• v.21 no.5
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• pp.455-461
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• 2002

This paper presents a method that improves the performance of the personal voice dialling system in which speaker independent phoneme HMM's are used. Since the speaker independent phoneme HMM based voice dialing system uses only the phone transcription of the input sentence, the storage space could be reduced greatly. However, the performance of the system is worse than that of the system which uses the speaker dependent models due to the phone recognition errors generated when the speaker independent models are used. In order to solve this problem, a new method that jointly estimates transformation vectors for the speaker adaptation and transcriptions from training utterances is presented. The biases and transcriptions are estimated iteratively from the training data of each user with maximum likelihood approach to the stochastic matching using speaker-independent phone models. Experimental result shows that the proposed method is superior to the conventional method which used transcriptions only.