• 원예과학기술지
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• 제17권6호
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• pp.742-746
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• 1999

8가지의 대목종류에 접목 또는 무접목한 '금동이' 참외와 '통일황' 참외의 목부액내 무기성분 및 시토키닌류 함량을 분석하여 비교하였으며, 이와 더불어 과실특성을 조사하였다. 과실의 경도는 대목 또는 접수에 따라서 차이를 보였으며, 과실의 태좌부의 당도는 '통일황'에서 높았다. 목부액의 전기전도도(EC)는 무접목묘에서는 '금동이'에서, 접목묘에서는 '통일황'에서 높았으며, 개체당 목부액의 분비량은 '금동이'에서 많았다. 목부액내의 무기성분함량은 접수 및 대목의 종류에 따라 차이를 보였고, 접목묘에서 무접목묘보다 높았는데 특히 $NO_3{^-}$와 $PO_4{^-}$의 함량에서 증가를 보였다. 이러한 접목에 의한 무기성분 함량의 증가 효과는 '통일황'에서 뚜렷하였다. 목부액내의 시토키닌류는 trans-zeatin(t-Z), trans-zeatin riboside(t-ZR), dihydrozeatin riboside(DHZR)와 소량의 isopentenyl adenine(IPA) 및 isopentenyl adenine riboside(IPAR)가 검출되었으며, 목부액내의 주요 시토 키닌은 t-ZR로 가장 높은 비율을 차지하였고, 다음으로 t-Z가 풍부하였다. 시토키닌 함량은 '금동이'에서는 '참토좌'대목에서 함량이 가장 높았으나, 대목에 따른 현저한 차이를 보이지 않았던 반면에, '통일황'에서는 대목의 종류에 관계없이 접목에 의한 증가를 보였다.

• 한국작물학회지
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• 제48권1호
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• pp.8-12
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• 2003

To find out the relationship between pod development and cytokinin contents during reproductive stage of peanut, the cytokinin contents, trans-zeatin ribo-side (t-ZR) and dihydrozeatin riboside (diZR), were investigated at 0, 7, 14, 21 and 28 days after flowering (DAF). The amounts of t-ZR and diZR in cotyledon and first branch among primary branches were 3, 448 pmol/g (FW) and 4, 824 p/g (FW), respectively, which were higher than those of other branches. The t-ZR and diZR contents of lower parts on the branch from cotyledon node at 7 DAF were 579 pmol/g (FW) and 2, 028 pmol/g (FW), respectively, which were higher than those of upper parts. The cytokinin contents of reproductive organs as flowering progressed were increased at 0 and 14 DAF on branch and position of node. The cytokinin contents of upper part with pruning the lower part on the branch from cotyledon node were high 112-337% at 7 DAF and 14 DAF compared with those of the control. In case of remove the upper part of the first internode on main axis, t-ZR contents was 4.7 times higher than diZR contents at 7 DAF. The pod setting rate of flower and position on the branch from cotyledon node was closely related to the cytokinin contents during floral reproductive stage.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1622-1628
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• 2006

Bacteria from the Methylobacterium genus, called pink-pigmented facultative methylotrophic bacteria (PPFMs), are common inhabitants of plants, potentially dominating the phyllosphere population, and are also encountered in the rhizosphere, seeds, and other parts of plants, being versatile in nature. The consistent success of the Methylobacterium plant association relies on methylotrophy, the ability to utilize the one-carbon compound methanol emitted by plants. However, the efficiency of Methylobacterium in plant growth promotion could be better exploited and thus has attracted increasing interest in recent years. Accordingly, the present study investigated the inoculation effects of Methylobacterium sp. strains CBMB20 and CBMB 110 on seed imbibition to tomato and red pepper on the growth and accumulation of phytohormone levels under gnotobiotic conditions. Seeds treated with the Methylobacterium strains showed a significant increase in root length when compared with either the uninoculated control or Methylobacterium extorquens $miaA^-$ knockout mutanttreated seeds. Extracts of the plant samples were used for indole-3-acetic acid (IAA), trans-zeatin riboside (t-ZR), and dihydrozeatin riboside (DHZR) assays by immunoanalysis. The treatment with Methylobacterium sp. CBMB20 or CBMB 110 produced significant increases in the accumulation of IAA and the cytokinins t-ZR and DHZR in the red pepper extracts, whereas no IAA was detected in the tomato extracts, although the cytokinin concentrations were significantly increased. Therefore, this study proved that the versatility of Methylobacterium as a plant-growth promoting bacteria could be better exploited.

• Journal of Plant Biology
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• 제38권2호
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• pp.143-151
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• 1995

Addition of plant growth hormones [0.01 mg/L NAA and 0.2mg/L benzyladenine (BA)] to a woody plant medium stimulated the adventitious bud formation of poplar explants during culture. Endogenous IAA content increased rapidly at the initial culture stage and then decreased, being followed by rapid increment again at the late culture. But the content of trans-zeatin riboside (t-ZR) increased continuously during the culture. Cytoplasmic soluble proteins were analyzed by one- and two-dimensional SDS-PAGE. Increased amount of 40 kD band was detected by one-dimensional electrophoresis using Coomassie Blue staining during the culture and two distinctive proteins whose mol wt is 40,000 were detected by two-dimensional electrophoresis using autoradiography and these proteins were synthesized continuously prior to the adventitious bud formation. When the midvein segments were transferred to the actinomycin D-containing medium, the spots of adventitious bud-related proteins(ABRPs) did not disappeared but weakened in intensity. So, it is concluded that genes coding for the ABRPs are regulated to some degree at the transcriptional level. Also, they were not observed in BA-free medium, suggesting that these proteins be regulated by cytokinin, which made then possible to form the adventitious bud.