• 제목/요약/키워드: traditional korean medical physiology

검색결과 239건 처리시간 0.018초

Current strategies using 3D organoids to establish in vitro maternal-embryonic interaction

  • Islam Mohamed Saadeldin;Seif Ehab;Ahmed Elsayed Noreldin;Ayman Abdel-Aziz Swelum;Seonggyu Bang;Hyejin Kim;Ki Young Yoon;Sanghoon Lee;Jongki Cho
    • Journal of Veterinary Science
    • /
    • 제25권3호
    • /
    • pp.40.1-40.19
    • /
    • 2024
  • Importance: The creation of robust maternal-embryonic interactions and implantation models is important for comprehending the early stages of embryonic development and reproductive disorders. Traditional two-dimensional (2D) cell culture systems often fail to accurately mimic the highly complex in vivo conditions. The employment of three-dimensional (3D) organoids has emerged as a promising strategy to overcome these limitations in recent years. The advancements in the field of organoid technology have opened new avenues for studying the physiology and diseases affecting female reproductive tract. Observations: This review summarizes the current strategies and advancements in the field of 3D organoids to establish maternal-embryonic interaction and implantation models for use in research and personalized medicine in assisted reproductive technology. The concepts of endometrial organoids, menstrual blood flow organoids, placental trophoblast organoids, stem cell-derived blastoids, and in vitro-generated embryo models are discussed in detail. We show the incorportaion of organoid systems and microfluidic technology to enhance tissue performance and precise management of the cellular surroundings. Conclusions and Relevance: This review provides insights into the future direction of modeling maternal-embryonic interaction research and its combination with other powerful technologies to interfere with this dialogue either by promoting or hindering it for improving fertility or methods for contraception, respectively. The merging of organoid systems with microfluidics facilitates the creation of sophisticated and functional organoid models, enhancing insights into organ development, disease mechanisms, and personalized medical investigations.

EBM 기반구축을 위한 사물탕 연구 문헌 분석 (Analysis of Studies on Samul-tang for Fundamental Establishment of Evidence Based Medicine)

  • 김정훈;이준경;하혜경;서창섭;이호영;정다영;이남헌;이진아;황대선;신현규
    • 동의생리병리학회지
    • /
    • 제23권4호
    • /
    • pp.779-788
    • /
    • 2009
  • To establish the fundament for EBM of Traditional Korean Medicine, the papers on Samul-tang which was frequently used in medical institutions of Traditional Korean Medicine were analyzed through researching domestic and international literatures. The papers were classified by the registration of domestic or international journals, by the year of publishment, by experimental methods, by laboratory animals used in biological experiment and by the kinds of studies on biological efficacy. Of total 67 papers on Samul-tang, 58 volumes were registered in domestic journals and 9 volumes were in international journals of which 8 volumes were in SCI journals. Since 1978, publishments of papers have continuously increased. The papers on instrumental analyses were 6, biological studies were 58 volumes, clinical studies were 3. Instrumental analyses were preceeded with standard compounds(gallic acid, albiflorin, paeoniflorin, benzoic acid, ferulic acid, 5-HMF). And biological studies showed improvement of cardiovascular function and circulation, antianemia, brain protection, immunoregulation, antistress, radioprotection, antifatigue, antiinflammation and antiallergy, antioxidative effect. Through clinical studies, antifatigue, improvement of insomnia and osteoporosis were reported. Samul-tang could be used to tonify and activate blood. And further study on clinical field need to be conducted in accordance with biological study.

한방 기능성 입욕제 SP1과 SP2의 항산화, 항노화, 미백효과에 관한 연구 (Study on the Antioxidative, Antiaging and Whitening Effects of Bathing Aid SP1 and SP2)

  • 정보경;박소정;김정수;한승헌;손경우;윤미영;이선경;이상재;김병주;권영규;채한
    • 동의생리병리학회지
    • /
    • 제24권5호
    • /
    • pp.837-842
    • /
    • 2010
  • The purpose of this study is to develop bathing aids as a strategic products to promote the medical tourism in Sancheong Jirisan Oriental medicinal herbs special district using medicinal herbs produced in Sancheong province, and to verify the effect of the bathing aids in vitro. We investigated the cytotoxicity activity, antioxidant activity, antiaging and whitening effects of Sanchung-PNU 1 (SP1) and Sanchung-PNU 2 (SP2) made with traditional medicinal herbs. The cytotoxicity activity was measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Antioxidant activities were determined by DPPH (1.1-diphenyl-2-picrylhydrazyl) radical scavenging capacity assay. We measured the inhibitory effect against tyrosinase activity to prove the whitening effect, and the inhibitory effect against elastase activity to prove the anti-aging effect. Two proposed prescriptions, SP1 and SP2, showed not significant cytotoxicity but significant (p<0.001) improvement in anti-oxidation, anti-wrinkle, and whitening effects compared to the control group. The result shows that these bathing aids have excellent DPPH radical scavenging effect and significant inhibitory effect against elastase and tyrosinase activity. These findings suggest that these bathing aids have a strong antioxidant, anti-aging, and whitening effect.

사상체질 진단검사를 위한 데이터마이닝 알고리즘 연구 (Data mining Algorithms for the Development of Sasang Type Diagnosis)

  • 홍진우;김영인;박소정;김병철;엄일규;황민우;신상우;김병주;권영규;채한
    • 동의생리병리학회지
    • /
    • 제23권6호
    • /
    • pp.1234-1240
    • /
    • 2009
  • This study was to compare the effectiveness and validity of various data-mining algorithm for Sasang type diagnostic test. We compared the sensitivity and specificity index of nine attribute selection and eleven class classification algorithms with 31 data-set characterizing Sasang typology and 10-fold validation methods installed in Waikato Environment Knowledge Analysis (WEKA). The highest classification validity score can be acquired as follows; 69.9 as Percentage Correctly Predicted index with Naive Bayes Classifier, 80 as sensitivity index with LWL/Tae-Eum type, 93.5 as specificity index with Naive Bayes Classifier/So-Eum type. The classification algorithm with highest PCP index of 69.62 after attribute selection was Naive Bayes Classifier. In this study we can find that the best-fit algorithm for traditional medicine is case sensitive and that characteristics of clinical circumstances, and data-mining algorithms and study purpose should be considered to get the highest validity even with the well defined data sets. It is also confirmed that we can't find one-fits-all algorithm and there should be many studies with trials and errors. This study will serve as a pivotal foundation for the development of medical instruments for Pattern Identification and Sasang type diagnosis on the basis of traditional Korean Medicine.

골다공증 치료법과 천연물을 이용한 대체요법 (Current Medical Therapies for Osteoporosis and Its Alternative Treatments Using Natural Products)

  • 오승훈;안순철
    • 생명과학회지
    • /
    • 제25권1호
    • /
    • pp.113-120
    • /
    • 2015
  • 골다공증은 골밀도(bone mineral density, BMD)가 평균 성인 정점에서 2.5 이상의 표준편차가 감소되는 뼈의 질환으로서 나이가 들어가면서 점차 증가하고 있다. 골다공증은 뼈를 흡수하는 파골세포와 뼈를 형성하는 조골세포로 이루어진 bone remodeling system의 불균형 때문에 발생한다. 이 불균형의 가장 큰 원인은 여성 폐경기 후에 따르는 에스트로겐 결핍 때문이다. 현재 골다공증의 치료에 사용되는 약들로는 호르몬 대체요법(hormone replacement therapy, HRT), biphosphonate, teriparatide 등이 있지만, 여러 가지 부작용 때문에 그들의 안정성과 실용성엔 의문의 여지가 있다. 더 안전한 대안을 찾기 위해 현재 천연물을 사용한 여러 가지 치료법이 연구되고 있다. Lactoferrin, isoflavone 등과 한약재를 이용한 많은 전통 치료법들이 있으며, 이는 뼈 흡수를 막거나, 뼈 동화를 일으킴으로써 골다공증 치료제로서의 가능성을 보여주고 있다. 그러나 대부분의 천연물 치료법은 지난 10여년간 괄목할만한 발전에도 불구하고 그 효능을 증명하기 위한 임상 예비단계에 머물고 있다. 따라서 천연물의 전임상 연구와 후속 임상 연구를 통해 새로운 골다공증 치료법으로 소개될 것이다.

한국형 중풍변증 지표에 대한 신뢰도 연구 (Study on the Standardization of Korean Pattern Identification for Wind Stroke)

  • 이선우;강병갑;강백규;한덕진;이정욱;신선호;문병순;이인
    • 동의생리병리학회지
    • /
    • 제22권2호
    • /
    • pp.453-458
    • /
    • 2008
  • This study aimed to develop an evaluation reliability of Korean pattern identification for wind stroke. We studied 643 patients with stroke and made a list of registry for each of them. The present study analyzed 553 cases, in which the resident’s pattern identification agreed with the specialist’s one, and the cases included five differentiation pattern: the fire-heat pattern (114), the dampness-phlegm pattern (157), the static blood pattern (11), the Yin deficiency pattern (81), and the Qi deficiency pattern (190). This study showed that none of the Cronbach's alpha reached 0.700, which is the general reliable level. The average Cronbach's alpha of each symptoms was 0.353 for the dampness-phlegm pattern, 0.571 for the fire-heat pattern, 0.443 for the Qi deficiency pattern, 0.451 for the Yin deficiency pattern, and 0.302 for the static blood pattern. This suggests the possibility that each pattern identification could be coincided with other symptoms, and it also shows the limits of pattern identification of this study that narrows the symptoms of paralysis patients into only a single pattern. Continuous compliments and researches should be done referring to this matter. However, the internal consistency analysis of all the pattern identification showed that every Cronbach's alpha were within the range of 0.670 to 0.703, and the Cronbach's alpha of the whole symptoms was evaluated as 0.692, which makes the reliability of the pattern identification as itself almost satisfactory to the general reliable level, and therefore, significant. In the future, continuous clinical research to develope this pattern identification for wind stroke actually applicable to stroke patients needs to be made through accumulating more cases, improving the objectivity.

"동의보감(東醫寶鑑)" "내경편(內景篇)"의 포(胞), 소변(小便), 대편(大便)에 나타난 질병(疾病)의 변증화(辨證化) 연구 (Study on Mechanistic Pattern Identification of Disease for Uterine, Urine and Excrements Parts of DongEuiBoGam NaeGyungPyen)

  • 김영목
    • 동의생리병리학회지
    • /
    • 제24권5호
    • /
    • pp.727-736
    • /
    • 2010
  • This study is about researching mechanistic pattern identification of disease for DongEuiBoGam NaeGyungPyen by analysing with pattern identification of modern Traditional Korean medical patholgy as more logical, systematic and standardized theory. Disease pattern mechanisms of uterine, urine and excrements parts of DongEuiBoGam NaeGyun gPyen in NaeGyungPyen of DongEuiBoGam are these. Menstrual irregularities in DongEuiBoGam can be classified flui d-humor depletion, blood deficiency, qi deficiency, qi stagnation, qi stagnation complicated by heat, blood stasis, blood deficiency complicated by heat, syndrome of heat entering blood chamber, syndrome of cold entering blood chamber. The disease pattern of abdominal pain after menstruation in DongEuiBoGam is blood deficiency complicated by heat, and a dysmenorrhea represents blood stasis with heat, fluid-humor deficiency. Advanced menstruation represent dual heat of the qi and blood, delayed menstruation is blood deficiency. The disease pattern of inhibited urination in DongEuiBoGam can be classified deficiency heat pattern of kidney yin deficiency(yin deficiency with effulgent fire), kidney qi deficiency, yin deficiency with yang hyperactivity, fluid-humor depletion, spleen-stomach dual deficiency, and excess he at pattern of bladder excess heat. The disease pattern of urinary incontinence in DongEuiBoGam can be classified deficiency pattern of kidney-bladder qi deficiency, consumptive disease, lung qi deficiency, kidney yin deficiency(yin deficiency with effulgent fire), kidney yang deficiency and excess pattern of lower energizer blood amassment, bladder excess heat. And most of them are deficiency from deficiency-excess Pattern Identification. The disease pattern of diarrhea in DongEuiBoGam can be classified deficiency pattern of qi deficiency, qi fall, spleen yang deficiency, kidney yang deficiency and so on and excess pattern of wind-cold-summerheat-dampness-fire, phlegm-fluid retention, dietary irregularities, qi movement stagnation. And most of them are deficiency from deficiency-excess Pattern Identification. Like these, this study identify pattern of disease in DongEuiBoGam by mechanism of disease theory.

파골세포 분화에 복령 추출물이 미치는 영향 (Effect of Hoelen in RANKL-induced Osteoclast Differentiation)

  • 천윤희;곽성철;오재민;최민규;김정중;곽한복;이명수;전병훈;문서영
    • 동의생리병리학회지
    • /
    • 제26권3호
    • /
    • pp.320-324
    • /
    • 2012
  • Osteoporosis is an important public health issue in postmenopausal women. It is a major public health concern and is widely believed that osteoporosis results from imbalance between bone resorption and bone formation. Recently natural products from plants have been extensively studied as therapeutic drugs to treat and prevent various diseases. Hoelen (scientific name, Poria cocos) is a mushroom that is used in traditional Chinese medicine. Hoelen exhibits anti-inflammatory activity and has a protective effect on tumor progression. However, the effect of hoelen in osteoclast differentiation remains unknown. Thus, we examined the effect of hoelen in receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. Hoelen significantly inhibited RANKL-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) in dose dependent manner without toxicity. Also, we showed that hoelen significantly inhibited the mRNA expression of tartrate-resistant acid phophatase (TRAP) and nuclear factor of activated T cells 1 (NFATc1) in BMMs treated with RANKL. In Particular, hoelen greatly inhibited the protein expression of NFATc1. Ectopic expression of NFATc1 partially reverses hoelen-mediated inhibition of osteoclast differentiation. Taken together, our results demonstrated that hoelen may be useful treatment option of bone-related disease such as osteoporosis, reumatoid arthritis, and periodontitis.

알코올성 간염의 변증설문 개발에 관한 연구 (Study to Develop the Pattern Identification Questionnaire for Alcoholic Hepatitis)

  • 김정은;박상은;이재왕;손호영;이병권;신철경;이수영;김원일;홍상훈;김보경;지규용;강창완;이인선
    • 동의생리병리학회지
    • /
    • 제23권5호
    • /
    • pp.958-963
    • /
    • 2009
  • I Alcoholic hepatitis is a serious liver disease that may lead to cirrhosis and carcinoma, and the short-term mortality rate is fairly high in severe patients. This study was conducted to develop the instrument of pattern identification for alcoholic hepatitis. We made the pattern identification questionnaire and symptoms indicator through reviewing traditional oriental medical literatures and got advices from the advisor committee with Delphi technique. The advisor committee on this study was organized by 10 professors of internal medicine of oriental medical colleges nationwide. The questionnaire was composed of questions about 6 pattern identification - dampness, heat, liver, spleen, cold and dryness. We gave importance to each symptoms of 6 pattern identification which had been scored on a 5-point scale. We surveyed two groups: 36 male alcoholic hepatitis patients whose Alcohol Use Disorder Identification Test(AUDIT) scores were over 12 and who drank alcohol over 40 g per day were allocated to the hepatitis group. Forty three men who did not drink alcohol were allocated to the normal group. Alcoholic hepatitis had relativities to dampness, heat among cause of disease and liver, spleen among viscera. There were statistical significances between the hepatitis group and the normal group in dampness, heat, liver questionnaire. As a result of this study we suggest that the questionnaire would be effective instruments of pattern identification for alcoholic hepatitis.

한의학의 항종양 면역치료에 관한 연구 -1990년 이후 발표된 실험논문을 중심으로- (Compilation of 104 Experimental Theses on the Antitumor and Immuno-activating therapies of Oriental Medicine)

  • 강연이;김태임;박종오;김성훈;박종대;김동희
    • 동의생리병리학회지
    • /
    • 제17권1호
    • /
    • pp.1-24
    • /
    • 2003
  • This study was done to compile 104 experimental theses which are related to the antitumor and immuno-activating therapies between February 1990 through February 2002. Master's and doctoral theses were dassified by schools, degrees, materials, effects, experimental methods of antitumor and immunoactivity, and results. The following results were obtained from this study : 1. Classifying the theses by the school, 34.6% were presented by Daejeon University, 29.8% by Kyung-hee University and 11.5% by Won-kwang University. Of all theses, 51.0% were aimed for the doctoral degree and 43.3% were for the master's degree. All of three universities have their own cancer centers. 2. Classifying the theses by herb materials, complex prescription accounted for 60.3%, single herb accounted for 24.8% and herbal acupuncture accounted for 14.2%. Considering the key principles of the traditional medicine, complex prescription was much more thoroughly studied than single herb prescription. The results showed that the complex prescription had both antitumor activity and immuno-activating activity, which might reflects on multi-activation mechanisms by complex components. 3. Classifying the theses by the efficacy of herbs examined, in single herb, invigorating spleen and supplementing was 35.5%, expelling toxin and cooling was 29.0%, activating blood flow and removing blood stasis was 12.9%. In herbal acupuncture, invigorating spleen and supplementing was 52.9%, expelling toxin and cooling was 29.4%. In complex prescription, pathogen-free status was 41.9%, strengthening healthy qi to eliminate pathogen was 35.5%, strengthening healthy qi was 22.6%. It is presumed that the antitumor and immunoactivating therapy based on syndrome differentiation is the best way to develop oriental oncology. 4. Classifying the theses by antitumor experiments, cytotoxic effect was 48.1 %, survival time was 48.1 % and change of tumor size was 42.3%. Survival rate was not necessarily correlated with cytotoxicity. These data reflect the characteristic, wholistic nature of the oriental medicine which is based on BRM (biological response modifier). 5. Classifying the theses by immunoactivating experiments, hemolysin titer was 51.0%, hemagglutinin titer was 46.2% and NK cell's activity was 44.2%. In the future studies, an effort to elucidate specific molecular and cellular mechanisms of cytokine production in the body would be crucial. 6. Classifying the theses according to the data in terms of antitumor activity, 50% was evaluated good, 24.0% was excellent, and 15.5% have no effect. In an evaluation of immuno-activating activity, 35.9% was excellent and 18.0% showed a little effect. The index point, as described here, may helps to use experimental data for clinical trials. Changes in index points by varying dosage implicate the importance of oriental medical theory for prescription. 7. In 167 materials, IIP (immunoactivating index point, mean : 3.12±0.07) was significantly higher than AIP(antitumor index point, mean : 2.83±0.07). These data demonstrate that the effect of herb medicine on tumor activity depends more on immunoactivating activity than antitumor activity. This further implies that the development of herbal antitumor drugs must be preceded by the mechanistic understanding of immunoactivating effect. 8. After medline-searching tumor and herb-related articles from NCBI web site, we conclude that most of the studies are primarily focused on biomolecular mechanisms and/or pathways. Henceforth, we need to define the biomolecular mechanisms and/or pathways affected by herbs or complicated prescriptions. 9. Therefore, the most important point of oriental medical oncology is to conned between experimental results and clinical trials. For the public application of herbal therapy to cancer, it is critical to present the data to mass media. 10. To develop the relationship of experimental results and clinical trials, university's cancer clinic must have a long-range plan related to the university laboratories and, at the same time, a regular consortium for this relationship is imperative. 11. After all these efforts, a new type herbal medicine for cancer therapy which is to take care of the long-term administering and safety problem must be developed. Then, it would be expected that anti-tumor herbal acupuncture can improve clinical symptoms and quality of life (QOL) for cancer patients. 12. Finally, oriental medical cancer center must be constructed in NCC (National Cancer Center) or government agency for the development of oriental medical oncology which has international competitive power.