Journal of Veterinary Science

  • 제25권3호
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  • Pages.40.1-40.19
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  • 2024
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Current strategies using 3D organoids to establish in vitro maternal-embryonic interaction

  • Islam Mohamed Saadeldin (Comparative Medicine Department, King Faisal Specialist Hospital and Research Centre) ;
  • Seif Ehab (Biomedical Sciences Program, University of Science and Technology, Zewail City of Science and Technology) ;
  • Ahmed Elsayed Noreldin (Department of Histology and Cytology, Faculty of Veterinary Medicine, Damanhour University, the Scientific Campus) ;
  • Ayman Abdel-Aziz Swelum (Department of Animal Production, College of Food and Agriculture Sciences, King Saud University) ;
  • Seonggyu Bang (College of Veterinary Medicine, Chungnam National University) ;
  • Hyejin Kim (Division in Biomedical Art, Department of Fine Art, Incheon Catholic University Graduate School) ;
  • Ki Young Yoon (Department of Companion Animal, Shingu College) ;
  • Sanghoon Lee (College of Veterinary Medicine, Chungnam National University) ;
  • Jongki Cho (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • 투고 : 2024.01.07
  • 심사 : 2024.03.28
  • 발행 : 2024.05.31
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초록

Importance: The creation of robust maternal-embryonic interactions and implantation models is important for comprehending the early stages of embryonic development and reproductive disorders. Traditional two-dimensional (2D) cell culture systems often fail to accurately mimic the highly complex in vivo conditions. The employment of three-dimensional (3D) organoids has emerged as a promising strategy to overcome these limitations in recent years. The advancements in the field of organoid technology have opened new avenues for studying the physiology and diseases affecting female reproductive tract. Observations: This review summarizes the current strategies and advancements in the field of 3D organoids to establish maternal-embryonic interaction and implantation models for use in research and personalized medicine in assisted reproductive technology. The concepts of endometrial organoids, menstrual blood flow organoids, placental trophoblast organoids, stem cell-derived blastoids, and in vitro-generated embryo models are discussed in detail. We show the incorportaion of organoid systems and microfluidic technology to enhance tissue performance and precise management of the cellular surroundings. Conclusions and Relevance: This review provides insights into the future direction of modeling maternal-embryonic interaction research and its combination with other powerful technologies to interfere with this dialogue either by promoting or hindering it for improving fertility or methods for contraception, respectively. The merging of organoid systems with microfluidics facilitates the creation of sophisticated and functional organoid models, enhancing insights into organ development, disease mechanisms, and personalized medical investigations.

키워드

과제정보

This work was supported by the Ministry of Science and ICT through the National Research Foundation of Korea (NRF) (grant numbers:2021R1A2C2009294 and 2022R1I1A1A01065412) and the Brain Pool Program (grant number:2021H1D3A2A02040098). The funding bodies have no role in the design of the study or the analysis and interpretation of data.

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