• Toxicological Research
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• 제31권3호
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• pp.289-297
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• 2015

Ethyl carbamate (EC) classified as a probable human carcinogen (Group 2A) is naturally formed in alcoholic beverages and fermented foods during fermentation process and/or during storage. The objective of this study was to analyze EC in 34 food items including 14 alcoholic beverages and 20 fermented foods sold in Korea. Each food was collected from 18 supermarkets in 9 metropolitan cities in Korea, and then made into composite. According to food composition and alcohol content, samples were divided into four matrices such as apple juice, milk, Soju (liquor containing about 20% alcohol), and rice porridge. The maximum EC value of $151.06{\mu}g/kg$ was found in Maesilju (liquor made from Maesil and Soju). Whisky and Bokbunjaju (Korean black raspberry wine) contained $9.90{\mu}g/kg$ and $6.30{\mu}g/kg$, respectively. EC was not detected in other alcoholic beverages. Of 20 fermented foods, Japanese-style soy sauce had highest level of $15.59{\mu}g/kg$ and traditional one contained $4.18{\mu}g/kg$. Soybean paste had $1.18{\mu}g/kg$, however, EC was not found in other fermented foods.

• 한국유가공학회:학술대회논문집
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• 한국유가공기술과학회 2004년도 제58회 춘계유가공심포지움 - 세계 우유의 날 기념 국제 심포지움
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• pp.21-39
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• 2004

Fermented milks have been recognized as healthy foods since ancient times, but those using intestinal bacteria such as Bifidobacterium and lactic acid bacteria (LAB) are even more valuable from the standpoint of view of maintaining health. They have also now come to be recognized as important in the field of preventive medicine. Although advances in the medical sciences in the last 50 years have significantly increased the human lift span, an unfortunate fact is that many of us are now living long enough to experience chronic disorders such as coronary heart disease, hypertension, osteoporosis, diabetes and cancer. In recent years there has been renewed interest in health promotion and disease prevention by incorporating probiotic bacteria into foods to counteract harmful bacteria in the intestinal tract. Therefore, there are now a wide variety of commercial products containing prospective probiotics that claim health-promoting effects, such reductions in large botvel carcinogens and mutagens, antitumor properties, cholesterol-lowering effects, increased lactose digestion, relief from con-stipation, stimulation of immunocomponent cells and enhancement of phagocytosis. Two well-known representative probiotic is LAB and Bifidobaclerium. Traditional probiotic dairy strains of LAB which have been designated as GRAS (Generally Recognized As Safe) bacteria have a long history of safe use and most strains are considered comestible microorganisms with no pathogenic potential. Accordingly, there is considerable interest in extending the range of foods containing probiotic organisms from dairy foods to infant formulas, baby foods, and pharmaceuticals. In addition, the ingestion of probiotics, prebiotics, and symbiotic as wll as combinations of pro- and prebiotics has recently aroused renewed interest as enhancing the beneficial relationship between the host and intestinal microflora in both healthy and diseased indivisuals. Non-communicable chronic diseases such as cancer, cerebral hemorrhage, is chemic heart disease, and diabetes mellitus has recently been recognized as adult diseases in Japan as well as other countries. and are considered to be inevitably associated with aging. These diseases occur as a result of individual life styles. The Japanes Government. Ministry of Health, Labor and Welfare has proposed substituting the term 'adult diseases' with 'lifestyle-related diseases'. It has emphasized the importance of prevention rather than treatment. since the well-known increase in the elderly population in Japan is predicted to result in a variety of socioeconomic problems. n this lecture on the Present status of fermented milk products in Japan, I will report a strategy for the development of fermented milk products in Japan from the standpoint of view of research in Japan on LAB and Bifidobacteria. They could play an important role in preserving human health by controlling intestinal microflora capable of producing toxic effects on the host.

• 한국식생활문화학회지
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• 제26권1호
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• pp.1-10
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• 2011

Rice alcoholic beverages, cheongju and beopju, which are recorded in "Koryodokyung", "Koryosageolyo", and "Koryosa", were used in national and royal ceremonies, and yakju was used in the Palgwanhoi ritual. In the late $11^{th}$century, King Munjong imported hwaju and haenginjabeopju from the royal family of the Song Dynasty. Alcoholic beverages in the early $12^{th}$century included the medical use for kings, such as gyehyangeoju, which the Emperor of the Song Dynasty sent to King Yejong, baekjainju, which was sent to King Myeongjong for his health, and yangju, which is goat milk fermented alcohol from the nomads in the northern regions. In the early$13^{th}$ century there was also dongrak, which is a horse-milk fermented alcohol, grape wine sent from Yuan to King Chungryeol in the late $13^{th}$ century, and sangjonju, a type of special cheongju sent from Yuan in the early $14^{th}$ century. Baekju from Yuan was recorded in oral traditions, which suggests that soju, which is distilled cheongju, was consumed in the late $14^{th}$ century. Gold and silver wares for alcoholic beverages had important political, social, and economic meanings as national gifts to other countries and internally as the king's royal gift to his subjects. In the late $14^{th}$ century, soju was prohibited, and the use of gold and silver wares for alcoholic beverages was banned at the same time. This study examined the historical characteristics of the use of traditional rice alcoholic beverages, the emotional preference for foreign alcoholic beverages, and the gold and silver wares used for alcoholic beverages Koryo Dynasty.

• 한국축산식품학회지
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• 제33권3호
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• pp.325-330
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• 2013

Kefir is traditional fermented milk produced by various lactic acid bacteria (LAB) and yeast, which produce lactic acid, ethanol, carbon dioxide, and other flavor compounds. The purpose of this study was to evaluate the effects of different commercial oligosaccharides, such as maltotriose, fructooligosaccharide (FOS), galactooligosaccharide (GOS), and isomaltooligosaccharide (IMO), on the fermentation properties of kefir. First, we determined the acidification kinetic parameters, such as $V_{max}$, $t_{max}$(h), $t_{pH5.0}$(h), and $t_f$(h) of fermented milk supplemented with 4% (w/w) of different oligosaccharides. The probiotic survival and chemical composition (pH, organic acids profile, and ethanol content) of kefir during fermentation were also measured. Compared to control fermentation, all oligosaccharides increased acidification rate and reduced the time to complete fermentation (pH 4.7). The addition of FOS, in particular, resulted in the lowest $t_f$(h) and the highest populations of LAB and yeast during fermentation. All oligosaccharides increased ethanol production during fermentation. Further, significant differences were observed in the formation rates of six organic acids during fermentation. This study provided comparative data on the properties of commercial oligosaccharides for kefir manufacturing. Consequently, FOS especially had the potential for adequate and effective oligosaccharides in commercial kefir for the improvement of cost- and time-effectiveness.

• 한국미생물·생명공학회지
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• 제45권1호
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• pp.12-18
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• 2017

전국에서 수집한 발효식품으로부터 단백질 분해 효소활성을 보유한 균주를 분리하였다. Skim milk를 첨가한 TSA 평판배지에서 투명환을 형성한 8점의 균주 중 김치에서 분리된 MBE/L865 균주가 대조구인 KCTC13112 균주에 비하여 약 2.6배의 효소활성을 나타내었으며 Bacillus subtilis로 동정하여 한국미생물보존센터에 KCCM43059 균주로 기탁하였다. B. subtilis KCCM43059 균주의 최적 배양조건은 $37^{\circ}C$, pH 8으로 대조구인 KCTC13112 균주의 비성장속도보다 우수하였다. 반응조건 $60^{\circ}C$, pH 6에서 $429.37{\pm}18.65U/mg$ protein의 최적 효소활성을 나타내었으며 동일조건에서 대조구 균주보다 효소활성이 우수하였다. 또한 B. subtilis KCCM43059 균주는 대조구 균주에 비하여 NaCl에 상대적으로 높은 내성을 나타내었다.

• 한국미생물·생명공학회지
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• 제47권1호
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• pp.25-33
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• 2019

Lactobacillus rhamnosus BFE5264, isolated from a Maasai fermented milk product ("kule naoto"), was previously shown to exhibit bile acid resistance, cholesterol assimilation, and adhesion to HT29-MTX cells in vitro. In this study, we re-annotated and analyzed the previously reported complete genome sequence of strain BFE5264. The genome consists of a circular chromosome of 3,086,152 bp and a putative plasmid, which is the largest one identified among L. rhamnosus strains. Among the 2,883 predicted protein-coding genes, those with carbohydrate-related functions were the most abundant. Genome analysis of strain BFE5264 revealed two consecutive CRISPR regions and no known virulence factors or antimicrobial resistance genes. In addition, previously known highly variable regions in the genomes of L. rhamnosus strains were also evident in strain BFE5264. Pairwise comparison with the most studied probiotic strain L. rhamnosus GG revealed strain BFE5264-specific deletions, probably due to insertion sequence-mediated recombination. The latter was associated with loss of the spaCBA pilin gene cluster and exopolysaccharide biosynthetic genes. Comparative genomic analysis of the sequences from all available L. rhamnosus strains revealed that they were clustered into two groups, being within the same species boundary based on the average nucleotide identities. Strain BFE5264 had a sister group relationship with the group that contained strain GG, but neither ANI-based hierarchical clustering nor core-gene-based phylogenetic tree construction showed a clear distinctive pattern associated with the isolation source, implying that the genotype alone cannot account for their ecological niches. These results provide insights into the probiotic mechanisms of strain BFE5264 at the genomic level.

• 미생물학회지
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• 제33권2호
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• pp.136-141
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• 1997

한국의 전통 발효식품인 된장에서 단백질 분해효소를 분비하는 미생물의 존재를 규명하기 위해, skim milk 한천배지를 이용하여 투명환을 보이는 균주들을 얻었고, 각종 생리 및 생화학적 검사, VITEK system, MIDI system을 통해 이들 중, JH-1, SH-5, SH-7의 3 strain이 Bacillus cereus임을 동정하였다. JH-1과 SH-5 균주가 분비하는 단백질 분해효소는 pH 9와 40^{\circ}C.$에서 최적활성을 보였으며, SH-7 균주는 pH 8과 50^{\circ}C.$에서 최적활성을 보였다. 또한 이들 3균주는 blood agar plate에서 용혈능력을 보였다.

• 한국조리학회지
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• 제20권6호
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• pp.91-101
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• 2014

본 연구에서는 우리나라 고유의 전통발효유인 타락을 이용하여 디저트식품으로 선호도가 높은 아이스크림 제품류을 제조하고(sherbet 1종, 아이스밀크 2종(IM-2, IM-4), 아이스크림 1종(IC-6)), 각 타락 아이스크림류의 품질 특성을 알아보고자 한다. 타락 아이스크림류의 믹스의 점도는 수분 함량이 가장 높은 Sherbet이 가장 낮았고, 고형분 함량이 가장 높은 IC-6이 유의적으로 높았다. 오버런은 10분에서는 유지방함량이 높았던 IC-6이 높은 값을 나타냈고, 최종 30분에서는 모든 시료간의 유의적 차가 없었다. 녹아내리는 정도는 IC-6이 가장 높았고, Sherbet이 가장 낮은 값으로 측정되었다. 유산균수는 Sherbet은 7.32 Log CFU/g으로 유의적으로 낮게 계측되었고, IM-2, IM-4, IM-6는 8.35~8.49 Log CFU/g으로 유의적 차이가 없었다. 타락 아이스크림류 관능적 평가에서 먼저 강도평가에서 우유향에서는 IC-6이 4.10으로 가장 높았고, 신맛은 Sherbet이 4.20, IM-2가 4.10으로 평가되었으며, 단맛은 IC-6이 5.05로 가장 높게 평가되었고, 쓴맛은 IC-6이 1.65로 낮게 평가 되었다. 크리미한 정도는 IC-6이 4.60으로 유의적으로 높게 평가되었고, 바디감도 5.05로 가장 높게 평가되었다. 외관, 맛, 풍미, 조직감의 선호도에서 IC-6이 다른 시료에 비해 유의적으로 높게 평가되었고, 전반적인 선호도에서 IC-6이 5.15로 가장 높게 평가되었으며, Sherbet이 3.75, IM-2가 3.05, IM-4가 2.50으로 평가되어 타락을 첨가한 아이스크림류는 유지방 함량이 높은 아이스크림이 가장 좋은 관능 선호도를 나타내었고, 유지방함량이 중간정도인 아이스밀크류에 비해 유지방 함량이 없는 Sherbet의 관능 선호도가 조금 더 높았다.

• 한국식품조리과학회지
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• 제22권1호
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• pp.22-29
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• 2006

삼백초 추출물을 0.4% 첨가한 요구르트의 제조시 유고형분 함량을 14%, 16%, 18%로 달리하여 pH 및 산도를 측정한 결과 각 구별 유의적인 차이를 보이지 않았으나, 점도의 경우 18% 첨가구에서 가장 높은 점도 값을 나타내어 드레싱의 제조시의 발효기질 함량을 18%로 결정하였다. 삼백초 요구르트를 첨가한 드레싱의 최적 제조공정조건을 알아보기 위하여 반응표면분석을 이용하였다. 삼백초 요구르트 드레싱 제조를 위해 가장 영향을 미치는 요인 변수로 요구르트 첨가량을 (30, 40, 50, 60 및 70 g), 마요네즈첨가량 (6, 12, 18, 24 및 30 g) 소금 (0.1, 0.3, 0.5, 0.7 및 0.9 g)에 대하여 중심합성계획을 하였다. 그리고 드레싱의 관능적 특성을 최적화하기 위해 각 제조 조건별 색, 향기, 맛, 조직감, 후맛, 전체적인 기호도에 대한 contour map을 superimposing하여 최적 제조조건 범위를 예측하였다. 그 결과 삼백초 요구르트 드레싱 제조의 최적배합비율은 요구르트 61.20%, 마요네즈 23.50%, 소금 0.58%, honey 11.75%, mustard 1.76%, tabasco pepper sauce 0.23%, white wine 0.94% 및 white pepper 0.04%로 나타났다.

• 한국식품영양과학회지
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• 제31권4호
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• pp.583-588
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• 2002

비지로부터 두 균주를 순수분리하여 동정한 결과 Ent. faecium LL과 L. rhmnosus LS로 확인되었다. 분리된 균을 대두 미세분말용액에서 발효정도를 알아보기 위하여 pH, 산도, 생균수 및 유기산 함량을 측정하였다 Ent. faecium LL은 대두미세분말 용액에서 발효시 pH 4.9, 산도 0.38%, 생균수 1.8$\times$$10^{9}$ CFU/$m\ell$로 젖산생성 및 높은 생균수를 나타내었다. L. rhamnosus LS는 대두미세분말 용액에서는 4.6$\times$$10^{8}$ CFU/mL의 생균수를 나타냈지만 젖산의 생성은 매우 미흡하였다. 그러나 대두미세분말 용액에 당을 첨가하거나 skim milk를 첨가할때 산생성이 급격히 증가되었다. 대두미세분말과 skim milk 4 : 1 혼합액에서 Ent. faecium LL과 L. rhamnosus LS에 의한 젖산발효는 37$^{\circ}C$에서 20시간 안에 curd를 형성하였으며, 각각 0.33% 및 0.77%의 산도와 $10^{8}$ ~$10^{9}$ CFU/$m\ell$정도의 생균수를 보였다. HPLC 분석에서 생성된 젖산의 농도는 L. rhamnosus LS가 600 mg%로 Ent. faecium LL의 350 mg%보다 높았다.