• 한국동물위생학회지
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• 제26권4호
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• pp.345-359
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• 2003

A number of toxicants have been incriminated as a causing hepatic disease. Among many detrimental injury, alcohol has been noted for hepatitis, fatty liver, fibrosis, and hepatic cirrhosis. The purpose of this study was to develop animal model for hepatic fibrosis in pigs fed ethanol, and to search for a new anti-fibrogenic agent via this model. Twelve male Landrace pigs were divided into 3 groups of 4 animals each. Group 1, 2 and 3 were fed with active ceramic water only, ceramic water + liquid diet containing 15% ethanol and normal tap water + liquid diet containing 15% ethanol for 12 weeks, respectively. At week 12, all pigs were immediately sacrificed for collection each tissue and blood. Serologically, serum ALT and AST levels were significantly reversed in group 2, as compared to group 3. They were normal range in pigs of group 1. Microscopically, macrovesicular lipid droplets and moderate hepatocellular necrosis were evident in the tap water + ethanol fed group 3. However, the active ceramic water treated group 1 showed normal architecture. Moreover, in group 2, mild fatty changes and necrosis were observed in hepatocytes. Collagen fibers were increased in spaces surrounding periportal and interlobular connective tissues in the group 3 of tap water + ethanol, but collagen synthesis and its thickness of fibrotic septa connecting portal tracts were markedly reduced in the group 2 of ceramic water + ethanol. Myofibroblasts were detected mainly in the interlobular connective tissues of pig liver of group 3 treated ethanol and tap water. Few to no myofibroblasts were observed in groups 1 and 2. CYP2E1 was not or rarely detected in group 1 fed ceramic water. However, group 2 showed slightly activation of CYP2E1 in the area of pericentral vein, while CYP2E1 was significantly activated in group 3 fed tap water and ethanol. Based on the above data, we believe that we have developed a unique alcohol induced fibrosis model in pig, which will be useful in developing anti-fibrotic agents and drugs. Furthermore, the active ceramic water used in our study had an inhibitory and may be protective against ethanol induced hepatic toxicity and fibrosis.

• 물과 미래
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• 제27권1호
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• pp.69-77
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• 1994

생활수준이 향상됨에 따라 세제의 사용량이 큰 폭으로 증가하고 있으며, 하수처리공정에서 완전히 처리되지 못한 합성세제가 하천이나 강으로 흘러들어 지표수의 오염을 가중시키고 있다. 이와같은 세제는 수중생물에 해로운 여러 독성물질을 함유하고 있으며, 현재까지는 주로 화학적 분석에 의하여 그 독성을 측정하고 있다. 그러나 오염물질 상호간의 작용 등으로 화학분석만으로 적정기간동안에 독성을 평가하는 것은 상당히 어려운 실정인 바, 본 논문에서는 물벼룩인 Daphnia magna와 형광성 박테리아인 Photobacterium phosphoreum을 이용하여 생물학적으로 주방용 세제와 의류용 세제의 급성 독성을 평가하였다. 본 평가에 사용된 세제는 세탁용 세제와 주방용 세제로 구분하여 국내 사용량이 가장 많은 7종류를 선택하였으며, 조사결과 세탁용 세제의 평균 24hr, 48hr-LC50은 각각 4.25%, 2.50%이며, 주방용 세제의 평균 24hr, 48hr-LC50은 각각 2.01%, 1.36%이었다. 또한 세탁용 세제의 평균 5min, 15min-EC50은 각각 1.83%, 1.02%이며, 주방용 세제의 평균 5min, 15min-EC50은 각각 1.57%, 1.21% 이었다. 이를 통하여 국내에서 사용되는 합성세제중 주방용 세제가 세탁용 세제보다 지속적 독성이 큰 것을 알 수 있었으며 세제마다 독성의 정도가 차이가 있었다.

• 한국환경농학회지
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• 제29권4호
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• pp.421-426
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• 2010

벤젠은 농약 노출 및 새집 증후군시에 나타나는 중요한 물질로 천식 및 알러지 등의 호흡 질환을 일으키는 물질로 알려져 있으나 폐 상피세포에 대한 자세한 효과는 알려져 있지 않고 있다. 본 실험에서는 폐 상피세포인 A549 세포를 이용하여 벤젠에 대한 효과를 알아보았다. 실험 결과 벤젠은 세포 생존율을 감소 시켰으며, 이러한 반응은 항산화제인 vitamin C 및 NAC 처리 시 차단되었다. 실제로 벤젠 처리시 산화성 스트레스 지표인 lipid peroxide 형성이 증가하였으며 이들 반응 역시 항산화제들에 의해 차단되었다. 한편 벤젠 처리시 세포 사멸 촉진 단백질인 Bax 발현은 증가하였으며 세포 사멸 억제 단백질인 Bcl-2의 발현은 억제 되었으며 세포 사멸 실행 단백질인 casapse-3의 활성형 역시 증가하였다. 결론적으로 벤젠은 폐 상피세포에서 산화성 스트레스 증가를 통해 세포 사멸을 일으키는 것으로 나타났다.

• 한국발생생물학회지:발생과생식
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• 제6권1호
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• pp.25-30
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• 2002

내분비계 장애물질은 다양한 동물의 생식과 배아의 발생에 위해한 영향을 미친다고 보고되었다. 이러한 초기 배아의 비정상 발생 중 대표적인 것이 할구파편이다. 본 연구에서는 착상 전 생쥐 초기 배아를 PCB, BPA, DDT에 체내, 체외에서 각각 노출시켰다. 내분비계 장애 물질에 노출시켰던 배아는 할구파편, 불규칙한 할구와 깨짐, 일부 파괴된 투명대 배아 등의 다양한 형태학적 비정상 양상을 보였다. 착상 전 생쥐 초기배아의 세포괴사에 관여하는 유전자를 조사하기 위하여 RT-PCR을 사용하여 mRNA수준에서 평가하였다. Bcl-2, bad 그리고 bax유전자 발현 정도를 대조군과 내분비계 장애물질 처리군으로 나누어 비교하였다. Bcl-2유전자는 내분비계 장애물질 처리군에서 낮아지는 경향을 보였고, bad와bix는 내분비계 장애물질 처리군에서 보다 높은 경향을 보였다. 이러한 연구 결과는 착상 전 생쥐 초기배아를 체내와 체외에서 내분비계 장애물질에 노출시킴으로써 발생적 독성물질을 신속히 판단할수 있는 유용한 수단이 될 수 있을 것으로 생각한다.

• 한국화재소방학회논문지
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• 제17권3호
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• pp.61-73
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• 2003

화학공장에서 발생되는 사고 중 대부분은 저장탱크나 운송배관의 손상에 의한 휘발성 유독성물질의 대량 누출이며, 이 경우 누출된 지역의 자연환경과 대기조건에 따른 유동성물질의 확산거동이 안전성평가의 가장 중요한 관심 대상이 된다. 따라서 본 연구에서는 이러한 누출물질에 대한 대기 중 확산을 모사하기 위하여 염소저장탱크에서 염소가 누출될 경우를 예제로 선택하여, 위험성평가와 확산모델(dense gas model)을 이용한 결과해석을 수행하였다. 해석결과를 살펴보면 Fire & Explosion Index를 적용한 결과 포괄적인 위험의 정도는 90.7로서 약간 위험한 정도로 나타났으며, 대기확산 모델(PHAST6.0/ALOHA)은 소프트웨어 운용한 결과, Gas Model에 대한 입력 자료 값에 따라 미치는 결과영향이 다소 차이가 있음을 발견하였으나 각 시나리오별 경향은 상당히 일치함을 나타내고 있다. 따라서 향후 보다 정확한 물성입력자료와 지형인자를 고려한다면 이와 같은 연구방법은 유독성물질 누출에 따른 위험성평가를 보다 효율적으로 수행하는데 도움을 줄 것으로 기대된다.

• 생태와환경
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• 제39권4호통권118호
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• pp.419-423
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• 2006

본 연구에서는 국내 토착종인 왜물개 (Aphyocypris chinensis)을 사용하여 4가지 농약(diazinon, iprodione, fenpropathrin, myclobutanil)의 급성독성을 평가하기 위하여 지수식으로 독성실험을 실시하여 96-hr $LC_{50}$ 값과 95% 신뢰한계를 구하였다. 각 실험농약의 96-hr $LC_{50}$ 값을 비교하여 왜몰개에 대한 급성독성이 높은 순서를 정리한 결과는 Fenproparthrin (0.003 mg $L^{-1}$)>Myclobutanil (9.1 mg $L^{-1}$)>Diazinon (14.1 mg $L^{-1}$)>Iprodione (31.8 mg $L^{-1}$) 순으로 나타났다. 본 연구에서 사용된 4가지 농약에 대한 왜물개의 급성독성 값은 현재까지 보고된 다른 어종의 급성독성 값과 차이가 있었다. 그러므로 환경관리 및 규제목적으로 어류에 대한 급성독성실험 결과를 수질기준 또는 물질분류에 이용하기 위해서는 다양한 어류에 대한 급성독성 평가를 통하여 종합적 판단이 필요하다고 사료된다.

• 한국축산식품학회지
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• 제37권1호
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• pp.79-86
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• 2017

Aflatoxin $M_1$ ($AFM_1$) after its bioconversion from aflatoxin $B_1$ in animal liver becomes the part of milk while heavy metals get entry into milk and milk products during handling in the supply chain. Aflatoxin $M_1$ and heavy metals being toxic compounds are needed to be monitored continuously to avoid any ailments among consumers of foods contaminated with such toxicants. Thirteen commercially available infant formula milk (IFM) brands available in Pakistani markets were analyzed for the quantitative determination of $AFM_1$ and heavy metals through ELISA and atomic absorption spectrophotometer, respectively. $AFM_1$ was found positive in 53.84% samples while 30.76% samples were found exceeding the maximum EU limit i.e. $0.025 {\mu}g/kg$ for $AFM_1$ in IFM. Heavy metals lead (Pb) and cadmium (Cd) were found below the detection limits in any of the sample, whereas the concentrations of iron (Fe), zinc (Zn) and nickel (Ni) ranged between 45.40-97.10, 29.72-113.50 and <$0.001-50.90 {\mu}g/kg$, respectively. The concentration of Fe in all the tested brands was found in normal ranges while the concentrations of Zn and Ni were found exceeding the standard norms. Elevated levels of $AFM_1$, Zn and Ni in some of the tested IFM brands indicated that a diet completely based on these IFM brands might pose sever health implications in the most vulnerable community i.e., infants.

• Safety and Health at Work
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• 제11권2호
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• pp.152-158
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• 2020

Background: The Globally Harmonized System of Classification and Labeling of Chemicals (GHS) was developed to enhance chemical classification and hazard communication systems worldwide. However, some of the elements such as building blocks and data sources have the potential to cause "disharmony" to the GHS, particularly in its classification results. It is known that some countries have developed their own lists of classified chemicals in accordance with the GHS to "standardize" the classification results within their respective countries. However, the lists of classified chemicals may not be consistent among these countries. Method: In this study, the lists of classified chemicals developed by the European Union, Japan, Malaysia, and New Zealand were selected for comparison of classification results for carcinogenicity, germ cell mutagenicity, and reproductive toxicity. Results: The findings show that only 54%, 66%, and 37% of the classification results for each Carcinogen, Mutagen and Reproductive toxicants hazard classes, respectively are the same among the selected countries. This indicates a "moderate" level of consistency among the classified chemicals lists. Conclusion: By using classification results for the carcinogenicity, germ cell mutagenicity, and reproductive toxicity hazard classes, this study demonstrates the "disharmony" in the classification results among the selected countries. We believe that the findings of this study deserve the attention of the relevant international bodies.

• Archives of Pharmacal Research
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• 제26권12호
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• pp.1047-1054
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• 2003

Molecular chaperones have a crucial role in the folding of nascent polypeptides in endoplasmic reticulum. Some of them are known to be sensitive to the modification by electrophilic metabolites of organic pro-toxicants. In order to identify chaperone proteins sensitive to alkyators, ER extract was subjected to alkylation by 4-acetamido-4 -maleimidyl-stilbene-2,2 -disulfonate (AMS), and subsequent SDS-PAGE analyses. Protein spots, with molecular mass of 160, 100, 57 and 36 kDa, were found to be sensitive to AMS alkylation, and one abundant chaperon protein was identified to be protein disulfide isomerase (PDI) in comparison with the purified PDI. To see the reactivity of PDI with cysteine alkylators, the reduced form ($PDI_{red}$) of PDI was incubated with various alkylators containing Michael acceptor structure for 30 min at $38^{\circ}C$ at pH 6.3, and the remaining activity was determined by the insulin reduction assay. Iodoacetamide or N-ethylmaleimide at 0.1 mM remarkably inactivated $PDI_{red}$ with N-ethylmaleimide being more potent than iodoacetamide. A partial inactivation of $PDI_{oxid}$ was expressed by iodoacetamide, but not N-ethylmaleimide (NEM) at pH 6.3. Of Michael acceptor compounds tested, 1,4-benzoquinone ($IC_{50}, 15 \mu$ M) was the most potent, followed by 4-hydroxy-2-nonenal and 1,4-naphthoquinone. In contrast, 1,2-naphthoquinone, devoid of a remarkable inactivation action, was effective to cause the oxidative conversion of $PDI_{red}$ to $PDI_{oxid}$. Thus, the action of Michael acceptor compounds differed greatly depending on their structure. Based on these, it is proposed that POI, one of chaperone proteins in ER, could be susceptible to endogenous or xenobiotic Michael acceptor compounds in vivo system.

• The Korean Journal of Physiology and Pharmacology
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• 제20권4호
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• pp.399-406
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• 2016

Early life neuronal exposure to environmental toxicants has been suggested to be an important etiology of neurodegenerative disease development. Perfluorohexanesulfonate (PFHxS), one of the major perfluoroalkyl compounds, is widely distributed environmental contaminants. We have reported that PFHxS induces neuronal apoptosis via ERK-mediated pathway. Imperatorin is a furanocoumarin found in various edible plants and has a wide range of pharmacological effects including neuroprotection. In this study, the effects of imperatorin on PFHxS-induced neuronal apoptosis and the underlying mechanisms are examined using cerebellar granule cells (CGC). CGC were isolated from seven-day old rats and were grown in culture for seven days. Caspase-3 activity and TUNEL staining were used to determine neuronal apoptosis. PFHxS-induced apoptosis of CGC was significantly reduced by imperatorin and PD98059, an ERK pathway inhibitor. PFHxS induced a persistent increase in intracellular calcium, which was significantly blocked by imperatorin, NMDA receptor antagonist, MK801 and the L-type voltage-dependent calcium channel blockers, diltiazem and nifedipine. The activation of caspase-3 by PFHxS was also inhibited by MK801, diltiazem and nifedipine. PFHxS-increased ERK activation was inhibited by imperatorin, MK801, diltiazem and nifedipine. Taken together, imperatorin protects CGC against PFHxS-induced apoptosis via inhibition of NMDA receptor/intracellular calcium-mediated ERK pathway.