• Applied Biological Chemistry
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• 제40권2호
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• pp.117-122
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• 1997

제주산 궁천조생의 저장조건에 따른 저온저장 특성을 검토하였다. 상온저장에 비해 저온저장 감귤은 저장기간 중 가용성 고형물, 총당, 비타민 C의 변화가 매우 적었으며, 부패율과 중량감소가 각각 10% 이내 수준에서 비교적 신선도를 유지한 상태에서 3월 하순까지 저장이 가능한 것으로 보였다. 또한, 중량감소, 경도의 저하, 당함량의 감소는 저장기간 중에 계속하여 서서히 일어났다. 필름포장과 wax-coating은 중량감소와 외관향상에는 도움을 주었으나 장기간 저장에는 부패율이 증가하였다. 습도조절을 하지 않은 저온저장은 습도조절한 경우와 비교하였을 때 부패율과 중량감소가 컸다. 증산작용으로 인한 껍질수분의 감소에 따라 과육으로부터 수분이동이 발생하여 저장기간 중 과육율이 감소하였으며, 저장 60일 후부터 경도저하가 많이 일어나 조직이 연화됨을 알 수 있었다. 조기수확한 감귤에 비하여 완숙한 감귤이 부패율과 중량감소가 적어 저장 100일간을 기준하여 출하하는 것이 바람직하였다. 상온저장한 감귤에 비해 저온저장한 감귤은 저장 4개월 이후 출고하였을 경우 외관 및 맛에 비교적 우수하였으며, 출고후 필름포장한 감귤의 부패율은 급속히 증가하여 취급에 주의가 필요하였다. 조생온주의 경우 저온에서 장기간 저장이 어려움을 알 수 있어서 저장용 감귤의 특성을 고려하여 저장조건을 설정할 필요가 있었다.

• 한국식품영양과학회지
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• 제32권1호
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• pp.29-34
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• 2003

가공식품의 개발에 있어서 식품의 맛과 더불어 저장성, 열안정성 및 색조유지는 소비자의 기호도에 중요한 영향을 미친다. B. subtilis EK11 유래의 PPase는 식물조직 중엽부의 주성분인 불용성 protopectin을 분해하여 단세포화하는 효소이다. PPase를 단감에 작용시켜 단감 고유의 세포 속에 함유되어 있는 세포내 성분들의 파손 없이 단세포를 유리하였다 PPase처리된 단감 단세포화물의 착즙 후 회수율과 잔사율은 각각 95%와 5%로서, 기계적 마쇄물에서 의 85%와 15%에 비하여 높은 회수율과 낮은 잔사율을 나타내었다. 총당, 환원당, 자당, 조단백질, 조지방 및 조섬유의 함량변화는 큰 차이가 없었으며, 이는 단세포 처리에 의하여 이들 성분이 안정하게 유지됨을 알 수 있었다. 식품중 열 또는 빛 에 가장 불안정한 비타민 C의 경우 단감 단세포화물은 1일 경 과 후에도 50% 이상이 보존되는 것으로 보아 단세포에 의한 일반적인 구성 성분이 안정하게 유지, 보호됨을 알 수 있다. PPase로 처리된 단감 단세포화물을 4$^{\circ}C$에서 9일간 저장하며 색조를 관찰한 결과, 단세포화물에서는 뚜렷한 색조의 차이가 없었고 기계적 마쇄물에서는 변색이 일어났다. 또한 단감 단세포화물을 10$0^{\circ}C$에서 60분간 열처리한 후 관찰한 결과, 기계적 마쇄물의 경우 짧은 처리에도 변화를 보였으나 단세포화물에서는 그다지 큰 변화가 없었으며 이는 효소적 단세포화물의 높은 열 안정성을 의미한다. PPase를 이용한 단감의 단세포화는 음료제조 및 원료보존에 유용하게 응용가능하며, 나아가 단감 단세포화물의 폭넓은 식품소재화 가능성과 고부가가치 기능성 식품제조에 이용될 수 있음을 의미한다.

• Journal of Periodontal and Implant Science
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• 제30권1호
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• pp.39-52
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• 2000

Polypeptide growth factor belong to a class of potent biologic mediator which regulate cell differentiation, proliferation, migration and metabolism. 1,25-dihydroxyvitamin $D_3$ decrease cell proliferation, and stimulate alkaline phosphatase activity which express in osteoblast during cell differentiation period. IGF-I is known to stimulate cell proliferation and differentiation too. 1,25-dihydroxyvitamin $D_3$ is known to increase IGF-I binding sites and IGF binding protein which inhibite the effect of IGF. The purpose of this study is to evaluate potential role of IGF-I as mediator that control the action of 1,25-dihydroxyvitamin $D_3$. MC3T3-E1 cell were seeded $5{\times}10^5/ml$ at 100mm culture plate in ${\alpha}-MEM$ containing 10% fetal bovine serum. After 48 hour incubation period, medium were changed ${\alpha}-MEM$ containing 5% fetal bovine serum. After 24 hours, $10^{-9}M$ 1,25-dihydroxyvitamin $D_3$ added. Total mRNA was extracted at 0, 6, 24, 48, 72 hour. PRPCR method was programed for the detection of IGF-I mRNA. In the both groups of 1,25-dihydroxy vitamin $D_3$ treated and control, alternative splicing form of IGF-I, IGF-IA and IGF-IB were expressed. In the 1,25-dihydroxyvitamin $D_3$ treated group, IGF-I mRNA expression was matained until 24 hour, there after expression was decresed. MC3T3-E1 cell were seeded $2.5{\times}10^4/ml$ at 24well plate in ${\alpha}-MEM$ containing 10% fetal bovine serum. After 48 hour incubation period, medium were changed ${\alpha}-MEM$ containing 3% fetal bovine serum. After 24 hours, $10^{-9}M$ 1,25-dihydroxyvitamin $D_3$ and 10 ng/ml IGF-I were added separately or together. Cell were cultured for 1 and 3 days, $2{\mu}Ci/ml\;[^3H]$ -thymidine was added for the last 24h of culture of each days. ${[^3H]}$-thymidine incorporation in to DNA was measured and expressed counter per minute(CPM). DNA synthetic activity was significantly decreased by 1,25-dihydroxyvitamin $D_3$ both at 1 day and 3 day, and in the combination group of 1,25-dihydroxyvitamin $D_3$ and IGF-I, DNA synthetic activity was also decreased both at 1 day and 3 days. IGF-I did not affect the DNA synthetic activity compared to control group both at 1 day and 3 day. From the above results, 1,25-dihydroxyvitamin $D_3$ was potent inhibitor of cell proliferaton in MC3T3-E1 cells. It assumed that the effect of 1,25-dihydroxyvitamin $D_3$ on osteoblast proliferation may be mediated in part by decreased level of IGF-I.

• 한국환경과학회지
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• 제29권5호
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• pp.469-477
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• 2020

This study analyzed the quality characteristics of black garlic made from Seosan Yukjok Garlic and elephant garlic in Seosan, Chungnam province. Of the inorganic components, Mg content was the highest in all treatment groups, and the Ca content was high in each of the 15 day treatments. The content of K was high after 10 days aging in Yukjok garlic and after 15 days in the elephant garlic. The Fe, Na, K, and Mg content was high in Yukjok black garlic after 15 days, and Na, K, Ca, and Mg were high in the elephant black garlic aged for 15 days. The crude fat content was high in both Yukjok black garlic and elephant black garlic after 15 days. Vitamin C content was highest in both types of garlic after aging for 15 days. An analysis of four kinds of organic acids showed that citric acid was the only organic acid to appear in raw garlic of Yukjok garlic and elephant garlic. Black Yukjok garlic and elephant black garlic had a greater total amino acid content than the raw garlic of either type. However, among the tested amino acids, 13 kinds of amino acids were at their highest after five days of ripening in Yukjok black garlic, while 15 kinds of amino acids were abundant in elephant garlic after the same period. Eight kinds of amino acids were high after aging for 15 days. Through this study, it was confirmed that, in the process of making black garlic, changes in the main components of the garlic occur through different routes, and these changes vary depending on the garlic species. Therefore, this study provided basic data for the processing of Seosan's Yukjok black garlic and elephant black garlic.

• 한국식품과학회지
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• 제32권5호
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• pp.1142-1148
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• 2000

소금절임 조건이 민들레 김치의 숙성과 품질에 미치는 영향을 조사하였다. 절임 민들레의 품질은 5% 염도에서는 16시간, 10%에서는 8시간 15%에서는 4시간 절임한 것이 양호하였으며, 염도별로는 10% 경우가 5 및 15% 경우보다 양호하였다. 그러나 다같이 쓴맛이 제거되지 않았다. 쓴맛의 제거는 10% 염도로 4일간, 1일 1회 염수를 교환함으로서 제거되었다. 10%의 소금물로 8시간동안 절임한 것(short-time salting: SS)과 동일 염도로 4일동안 절이면서 소금물을 1일 1회 교환한 것(long-time salting: LS)으로 구분하여 절임한후 $10^{\circ}C$에서 숙성시키면서 품질을 비교한 결과, SS 김치는 숙성 중 낮은 pH를 유지하였고 산도, 환원당와 비타민 C 함량 및 총균수와 젖산균수가 높아 LS 김치보다 숙성이 촉진되었다. 환원당 및 비타민 C 함량과 미생물의 수 및 관능검사 결과로 평가한 최적 숙성일은 SS 김치의 경우는 숙성 40일째였으나 LS김치는 50일째 이였다.

• Journal of Nutrition and Health
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• 제41권4호
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• pp.307-316
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• 2008

The purpose of the study was to investigate nutritional status of chronic obstructive pulmonary disease (COPD) patients and to find out the differences according to the stages of disease. From March to October, 2006, 41 stable male patients of mild to severe COPD patients were recruited from Seoul National University hospital. The patients' of body weight and fat free mass were assessed by bioelectrical impedance analysis. The nutritional status of the patients was also assessed by 3-day recall, index of nutritional quality (INQ), dietary diversity score (DDS), dietary variety score (DVS), food group index pattern and dietary quality index (DQI). The total of 41 patients were classified into three groups, stage I, stage II and stage III groups according to the classification of Global Initiative for Chronic Obstructive Lung Disease (GOLD) standard. The mean age of the patients in each stage were 67.2-66.9 years showing no significant difference. The ratio of $FEV_1$/FVC were $57.5{\pm}7.3$, $46.9{\pm}7.6$ and $38.2{\pm}6.8%$, respectively showing significant differences according to the stages of disease. The fat free mass of the stage II ($48.2{\pm}4.7kg$) and III ($47.3{\pm}4.5kg$) was significantly lower than that of stage I ($53.1{\pm}6.9kg$) patients. There were significant correlation of fat free mass with $FEV_{1}$, and BMI (body mass index) with $FEV_{1}$/FVC ratio (p < 0.05). COPD patients showed the diet-related clinical symptoms of anorexia, dyspnea, dyspepsia, and chewing difficulty. Daily intakes of calorie, K, vitamin $B_2$ and folate of the patients were very low ($83.8{\pm}20.7%$, $58.9{\pm}14.4%$, $70.7{\pm}19.6%$ and $74.4{\pm}10.2%$, respectively) however, they did not significantly different according to the stages of disease. Daily intake of calcium was significantly lower in the stage III patients (p < 0.05). The mean scores of dietary variety score was significantly lower in the stage III patients (p < 0.001). Dietary quality index of the patients were not different among the stages of disease and the scores indicated poor quality of diet. As a summary, we found that body fat free mass, regularity of exercise, frequency of having snacks and dietary variety score were significantly associated with the severity of chronic obstructive pulmonary disease.

• 한국조리학회지
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• 제20권1호
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• pp.55-64
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• 2014

블루베리를 열풍건조, 순환형 감압건조 및 동결건조의 방법으로 건조제품을 제조하여 품질특성과 생리활성을 비교조사 하였다. 일반성분 중 가장 많이 함유하고 있는 성분은 건조방법과 무관하게 탄수화물(68.48~80.00%)이었으며, 다음으로 수분(13.10~27.93%), 조단백질(1.63~5.41%), 조지방(0.58~1.25%) 및 회분(0.71~1.11%) 순으로 나타났다. pH는 순환형 감압건조시료의 pH가 유의적 차이가 없었으며, 갈변도의 경우 0.31~0.21의 값으로 건조 방법별로 차이가 많았다. 색도는 L값과 a값은 동결 건조한 시료가 더 높게 나타난 반면, b값의 경우 열풍, 순환형, 동결건조시료 순으로 높은 값을 나타내었다. 열풍 건조한 시료의 비타민 C 함량은 5.6 mg/100g이었으며, 순환형 감압건조 및 동결건조 시료의 비타민 C 함량은 6.9 mg/100g, 7.1 mg/100g으로 나타났다. 안토시아닌 함량은 10.08 mg/100g, 29.41 mg/100g, 33.87 mg/100g의 함량을 나타내어 안토시아닌 함량 역시 동결건조시료에서 더 높은 수치를 나타내었다. 열풍건조시료의 유리당 함량이 695.5 mg/100g으로 가장 높게 나타났다. 페놀함량과 DPPH 라디칼 소거능은 순환형 감압 건조 및 동결 건조가 높게 나타났다.

• 한국식생활문화학회지
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• 제25권4호
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• pp.389-399
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• 2010

This study was conducted in order to investigate and compare anthropometric measurements, bone density, nutrient intake, blood composition and food habits between non-smoking, non-alcohol drinking and smoking, alcohol drinking male university students in Seoul, South Korea. The data for food habits and health-related behaviors were obtained by selfadministered questionnaires. The BQIs of the subjects were measured by Quantitative Ultrasound (QUS). The subjects were divided into two groups: NSND (non-smoking and non-alcohol drinking, n=62) group and General (smoking and alcohol drinking, n=160) group. The results were analyzed using the SPSS program and were as follows: The average heights, weights, and BMIs of the two groups were 173.3 cm, 66.5 kg and 22.1 and 173.4 cm, 68.7 kg and 22.9, respectively. There were no differences between the groups regarding height, weight or BMI. SBP and DBP, however, were significantly higher in the general group than in the NSND group (p<0.01). The BQIs, Z-scores and T-scores of the two groups were 99.83, -0.23, and -0.31 and 98.24, -0.27 and -0.39, respectively, producing no significant differences between the two groups. The percentages for normal bone status, osteopenia and osteoporosis were 83.88%, 16.12% and 0.0% and 74.37%, 25.62% and 0.01%, respectively. Mean intakes of animal protein (p<0.05), animal fat (p<0.05), fiber (p<0.05), animal Ca(p<0.05), animal Fe (p<0.001), Zn(p<0.05), vitamin B1 (p<0.05) and niacin (p<0.05) were significantly different between the two groups, and mean serum levels of SGOT (p<0.01), SGPT (p<0.001), ${\gamma}$-GTP (p<0.001), triglycerides (p<0.01), total cholesterol (p<0.05) and hematocrit (p<0.05) were also significantly different between the two groups. Overall, there were no differences in meal regularity, frequency of snacking, reasons for overeating, exercise and defecation between the groups. However, favorite foods (p<0.05) and night-time meals (p<0.05) were significantly different. In conclusion, the health status of the NSND group was superior compared to the general group. Thus, students who smoke and alcohol drink should receive a practical and systematically-organized education regarding the increased health benefits of quitting smoking and alcohol drinking.

• 한방재활의학과학회지
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• 제33권3호
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• pp.47-65
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• 2023

Objectives We evaluated the wound healing effects of Dangguijakyak-san (DJ) using C57BL/6 mice that were generated open wound. Methods The study was conducted with seven C57BL/6 mice assigned to each group, divided into the normal group, control group, vitamin E group, DJ low-dose group, DJ high-dose group. We measured total polyphenol, flavonoid contents, the size of the wound, liver function, pro-inflammatory cytokine activity in serum, inflammation-related proteins, adhesion molecules and chemokine proteins, collagen-related proteins in skin tissue and histopathological changes by H&E and Masson's staining. Results DJ treatment significantly reduced the area of the wound compared to the control group. Also, inflammatory cytokines were reduced and the expression of anti-inflammatory-related factors (interleukin-4 [IL-4] and IL-10) was significantly increased in the DJ treatment group. We identified that DJ treatment inhibits both pathways of inflammation, the mitogen-activated protein kinases and nuclear factor-κB pathway. Moreover, the protein expressions of Sirt1 (sirtuin 1), MCP-1 (monocyte chemoattractant protein 1), ICAM-1 (intercellular adhesion molecule 1), and VCAM-1 (vascular cell adhesion molecule 1) were decreased by DJ administration. Also, the expression of α-smooth muscle actin and collagen type I alpha 1, collagen-related proteins, that help skin recovery was significantly increased in the DJ treatment group. Histopathologically, a relatively thin epithelial layer could be observed in the DJ administration group, as well as an increase in fibroblasts and collagen fibers. Conclusions These data suggest that DJ treatment is effective in wound healing, suppressing inflammatory proteins, increasing skin repair factors and improving histopathological changes caused by wounds.

• Journal of Nutrition and Health
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• 제8권4호
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• pp.15-26
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• 1975

제주도내 중학교 2학년 여학생 418명에게 일반적인 환경실태, 영양섭취 실태를 조사하고 성장발육과의 관계를 요약해 보면 다음과 같다. A. 조사대상자의 일반 실태 1. 형제의 수는 대부분이 $5{\sim}6$명이었다. 2. 유아기에는 90%가 모유로 양육되었으며 이유는 $7{\sim}12$개월이 20.9%이고 $13{\sim}24$개월이 45%였다. 3. 식사시간이 불규칙한 학생은 45.7%이며 불규칙한 이유는 식욕부족과 시간부족이 각각 42.9%, 31.9%였다. 4. 1년을 기준해서 병으로 결석하는 일수는 $2{\sim}5$일이 52%였다. 5. 기호 실태에 있어서는 주식으로는 잡곡밥이 45%, 식빵이 30.6%, 국수는 26.3%가 즐기고 40.7%나 흰밥을 제일 싫어하고 있다. 부식으로는 계란, 쇠고기, 닭고기, 김치류를 좋아하고 버터, 돼지고기, 나물류를 싫어한다. 간식은 아이스크림, 우유, 과일, 과즙, 빵 등을 좋아하고 호콩 등을 좋아하지 않는 것으로 나타났다. B. 영양섭취 실태 1. 열량섭취 평균은 1,738Ca1, 총 섭취열량에 대한 탄수화물의 비율은 73%, 단백질이 15%, 지방이 12% 로 나타났다. 2. 열량 및 모든 영양소가 권장량에 미달이나 vitamin A. $B_{1}$. Niacin 등은 권장량에 달하고 있다. 3. 단백질이 1일(日) 평균 섭취량은 68g 으로서 권장량 75g에 가깝다. 총 단백질에 대한 동물성 단백질이 비율은 36%로 나타났다. 4. 총식품 섭취량에 있어서 동물성 식품이 평균섭취 비율은 14%, 곡류가 63%를 차지하고 있다. C. 영양 섭취실태, 성장발육, 지능과의 상호관계 1. 열량 섭취량과 성장발육은 상호 의의(意義)있는 결과가 나타났다. 2. 단백질 섭취량과 성장발육도 상호관계가 의의(意義)있는 결과가 나타났다. 3. 열량과 단백질 섭취와 지능과의 상호관계는 의미가 없는 것으로 나타났다. 4. 이같은 상호 관계의 결과를 보면 영양과 성장발육은 직접적인 관계가 있다고 지적되고 반면 영양과 지능과의 관계는 간접적인 영향이 있을 뿐이라고 결론을 내릴 수 있다.