• Journal of the Optical Society of Korea
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• v.17 no.1
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• pp.81-85
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• 2013

A miniaturized fluorescence detection system based on total internal reflection (TIR) configuration, which is applicable to detecting the presence of biological materials labeled with fluorescence dye in micro total analysis systems (${\mu}TAS$), is proposed. In conventional fluorescence testing and analysis devices, interference between the excitation light beam and the emitted light from dyes is unavoidable. This paper presents a fluorescence detection system based on TIR configuration that allows the excitation light beam and the emitted light to be spatially perpendicular to each other so as to minimize the interference where fluorescence emission is detected at the orthogonal angle to the excitation beam. We achieved the limit of detection of about 5 nmol/L with a high linearity of 0.994 over a wide range of 6-FAM mol concentration, being comparable to that in earlier studies.

• Bulletin of the Korean Chemical Society
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• v.34 no.9
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• pp.2725-2730
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• 2013

Single C-reactive protein (CRP) molecules, which are non-specific acute phase markers and products of the innate immune system, were quantitatively detected on a gold-nanopatterned biochip using evanescent field-enhanced fluorescence imaging. The $4{\times}5$ gold-nanopatterned biochip (spot diameter of 500 nm) was fabricated by electron beam nanolithography. Unlabeled CRP molecules in human serum were identified with single-molecule sandwich immunoassay by detecting secondary fluorescence generated by total internal reflection fluorescence (TIRF) microscopy. With decreased standard CRP concentrations, relative fluorescence intensities reduced in the range of 33.3 zM-800 pM. To enhance fluorescence intensities in TIRF images, the distance between biochip surface and CRP molecules was optimally adjusted by considering the quenching effect of gold and the evanescent field intensity. As a result, TIRF only detected one single-CRP molecule on the biochip the first time.

• Journal of the Korean Society of Visualization
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• v.3 no.1
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• pp.3-19
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• 2005

Novel optical techniques are presented for three-dimensional tracking of nanoparticles; Optical Serial Sectioning Microscopy (OSSM) and Ratiometric Total Internal Reflection Fluorescent Microscopy (R-TIRFM). OSSM measures optically diffracted particle images, the so-called Point Spread Function (PSF), and dotermines the defocusing or line-of-sight location of the imaged particle measured from the focal plane. The line-of-sight Brownian motion detection using the OSSM technique is proposed in lieu of the more cumbersome two-dimensional Brownian motion tracking on the imaging plane as a potentially more effective tool to nonintrusively map the temperature fields for nanoparticle suspension fluids. On the other hand, R-TIRFM is presented to experimentally examine the classic theory on the near-wall hindered Brownian diffusive motion. An evanescent wave field from the total internal reflection of a 488-nm bandwidth of an argon-ion laser is used to provide a thin illumination field of an order of a few hundred nanometers from the wall. The experimental results show good agreement with the lateral hindrance theory, but show discrepancies from the normal hindrance theory. It is conjectured that the discrepancies can be attributed to the additional hindering effects, including electrostatic and electro-osmotic interactions between the negatively charged tracer particles and the glass surface.

• Near Infrared Analysis
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• v.1 no.2
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• pp.35-39
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• 2000

The purpose of this research was to find out suitable soil sample preparation and sample holding tools for NIR reflection radiation for estimating soil components. NIR reflectance was scanned at 2nm intervals from 1,100 to 2,500nm with an InfraAlyzer 500(Bran+Luebbe Co.). Coarse(2.0mm) and fine(0.5mm) soil sample and various sample holding tools were used to obtain mean diffuse reflection of the soil for the calibration and validation of the calibration set in estimating moisture, organic matter and total nitrogen of the soils. Multiple linear regression was used to obtain the best correlation of NIR spectroscopy method. Correlation of NIR spectroscopy method. Correlation of NIR spectra for finely and coarsely sized soil did not show much difference. The standard errors of prediction(SE) using different types of sample holding tools for organic matter, total nitrogen and soil moisture were better than 0.765, 0.041 and 0.63% respectively. From the results it can be concluded that NIR spectroscopy with flow type cell could be used as a fast routine testing method in quantitative determination of organic matter, total nitrogen and soil moisture.

• Korean Journal of Optics and Photonics
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• v.28 no.5
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• pp.229-235
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• 2017

A widely tunable U-shaped SGDBR (Sampled Grating Distributed Bragg Reflector) laser diode is designed and analyzed by means of a time-domain simulation. The U-shaped SGDBR laser diode consists of SGDBR, active, phase, and TIR (Total Internal Reflection) mirror sections, so the coupling losses across the sections should be carefully considered. The tuning range of the designed U-shaped SGDBR laser is about 1525-1570 nm, which is confirmed by the simulation. The simulation results show that the loss in the TIR mirror region should be less than about 2 dB, and the refractive-index difference at the butt coupling between the passive and active regions should be less than 0.1, to provide the complete tuning range.

• Journal of Environmental Science International
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• v.32 no.11
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• pp.801-809
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• 2023

The purpose of this study is to predict the reflection distance following to the pulsing pressure, total air supplying, filter bag size using numercial analysis techniques and use it as an efficient operation condition and economic data for off-line type pulse air jet bag filter. In this research, filtration area 6 m² condition, calculate filter resistance coefficient for simulation through the main experiments using coke dust. Ansys fluent V19.0 apply to CFD simulation, and analysis pulsing characteristics about pulsing pressure, filtration velocity and nozzle diameter. The maximum reflecting distance of off-line type pulse air jet bag filter is 1,000 mm regardless of total air supplying at over the 42 L/m² conditions, that indicates off-line type can extend filter bag length 1,000 mm than on-line type. In order to effective primary and secondary pulsing of off-line type pulse air jet bag filter, over the 5 bar of pulsing pressure and over the 42 L/m² of total air supplying are needed.

• Korean Journal of Optics and Photonics
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• v.7 no.3
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• pp.200-206
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• 1996

When a Gaussian beam is incident to a planar dielectric interface at an angle other than Brewster angle or the critical angle of total reflection, we derive the six nonspecular effects of rotation, lateral shift, focal shift, Rayleigh length change, magnitude and phase changes in the complex amplitude of the reflected beam simultaneously by taking account of the boundary condition. In the derivation we assume a Gaussian beam of fundamental mode to emerge from the interface and then match at the interface the constant, linear, and quadratic variations of the amplitude and phase of the reflected beam with those of the incident beam multiplied by the reflection coefficient. Our calculation shows that the six nonspecular effects can result from a linear variation of the natural logarithm of the reflection coefficient at the interface.

• Proceedings of the Optical Society of Korea Conference
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• 2008.02a
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• pp.141-142
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• 2008

In order to develop a simple ray tracing model for simulating the radiation pattern of a LED, the propagation of a ray within the LED capsule was systematically evaluated by considering refraction and reflection from the capsule-air interface, multiple reflection within the capsule, and total internal reflection at the boundary. It was found that concentric ring shapes on the radiation pattern was formed by the rays multiple-reflected from the capsule surface, and that the strength of these rays were decreased rapidly as the number of multiple reflection is increased.

• Atmosphere
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• v.18 no.3
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• pp.207-224
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• 2008

Severe downslope windstorms observed at Gangneung, Korea in the springtime during the last 30 years are studied to understand their generation mechanisms. 92 severe wind cases are selected for which the maximum instantaneous wind speeds exceed two standard deviation of total mean plus ($18.7ms^{-1}$). They are categorized into the three mechanisms (hydraulic jump, partial reflection, and critical-level reflection) proposed in previous studies based on the flow condition, which is calculated using the wind and temperature profile observed at one upstream rawinsonde station, Osan. Among the three, partial reflection is found to be the most frequent mechanism for the last 30 years (1976 - 2005). To understand the role of inversion in generating severe downslope windstorms, horizontal velocity perturbation was calculated analytically for the atmosphere with an inversion layer. It turned out that the intensity of downslope wind was increased by inversion layer of specific heights, which are well matched with the observations. For better understanding the generation mechanisms, two-dimensional numerical simulations are conducted for the 92 severe wind cases using the ARPS model. In most simulations, surface wind speed exceeds the value of the severe-wind criterion, and each simulated case can be explained by its own generation mechanism. However, in most simulations, the simulated surface wind speed is larger than the observed, due to ignoring the flow-splitting effect in the two-dimensional framework.

• Journal of the Korean Solar Energy Society
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• v.36 no.5
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• pp.1-8
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• 2016

In this paper, we evaluated the effect of a silica-based Anti-Reflection(AR) coating for PV modules. The coating technique can be easily applied to large-scale PV modules at room temperature with improvements of the optical properties that is qualified by the optical transmission measurements on the coated cover glass of the modules. The power improvement of the large-scale PV modules shows the increasing about 2.4% at standard condition of the coating technique on average. To improve the AR coating effect of the PV modules, we have characterized the individual PV modules by the measurements of DC power output, modified performance ratio(PRm) and the regression. The results show that the significant improvements of the AR coating effect are 6.4%, 5.5% and 4.5% of increasing of the performances by using the measurements of DC power output, modified performance ratio(PRm) and the regression, respectively.