• 한국식품위생안전성학회지
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• 제31권2호
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• pp.132-139
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• 2016

본 연구는 다류의 항산화 활성과 항산화 물질 함량에 대한 조사연구를 목적으로 하였다. 총 99건의 다류에 대하여 DPPH (1,1-diphenyl-2-picrylhydrazyl)의 자유라디칼소거에 대한 능력을 가지고 항산화 활성을 조사하였다. 항산화 활성은 차 시료 100 g당 아스코르빈산 당량의 mg(L-아스코르빈산 동등한 항산화 능력, AEAC)과 1회 제공량 당 mg(자유라디칼 소거활성, $FSC_{50}$)으로 나타냈고 시료추출액에서의 폴리페놀 함량은 Folin-Ciocalteu법으로, 플라보노이드의 함량은 Davis변법으로 측정하였고 Vitamin C는 HPLC법으로 분석하였다. AEAC 값은 마테차, 녹차, 홍차, 헛개차, 국화차, 우엉차에서 상대적으로 높은 항산화 활성을 보였다. 폴리페놀 물질은 차에서 자연스럽게 발생하는 중요한 항산화 물질로 홍차, 녹차 그리고 마테차에서 높은 함량을 나타냈다. 플라보노이드는 마테차, 홍차 그리고 마차에서, Vitamin C는 유자차와 녹차에서 높은 함량을 나타냈다. 유형별로는 침출차가 고형차나 액상차보다 높은 항산화 활성을 나타냈다. 연구결과, 다류에는 폴리페놀 함량과 항산화 활성사이에는 높은 상관관계가 있었고 플라보노이드와 항산화 활성사이에는 통계적으로 유의한 상관 관계가 있었다. 즉, 다류의 항산화 활성은 폴리페놀에 의한 영향으로 확인되었다.

• Journal of Nutrition and Health
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• 제47권6호
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• pp.484-488
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• 2014

Purpose: This study investigated the question of whether the addition of natural ingredients (pear fermented solution, celery powder, vitamin C) in curing agents may influence antioxidant activities and nutritional characteristics of smoked duck. Methods: Smoked duck samples with general or natural curing agent containing three additive ingredients were examined to determine total polyphenol contents, antioxidant activities, pH, TBARS, and food additives residue (nitrite ion, antioxidant, sodium glutamate). Results: Smoked duck with natural curing agent showed a higher level of total polyphenol content and antioxidant activities than smoked duck using general curing agent (p < 0.05). The pH and TBARS of smoked duck were significantly decreased by the addition of natural curing ingredients compared to those of smoked duck using general curing agent (p < 0.05). The residues of food additives were not detected in smoked duck using natural curing agent. Conclusion: Findings suggest that the addition of pear fermented solution, celery powder, and vitamin C in natural duck curing agent may improve the antioxidant activities and nutritional characteristics of smoked duck and provides health benefits.

• KSBB Journal
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• 제23권5호
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• pp.387-391
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• 2008

본 연구에서는 건조된 녹차 뿌리와 씨에 함유된 성분중에서 무기성분, 비타민, 총 아미노산과 유리 아미노산, 총 폴리페놀 및 그 구성 성분들의 함량을 분석하였다. 뿌리와 씨의 무기성분 중에서는 K의 함량이 각각 1,052 mg/100 g과 1,480 mg/100 g으로 가장 많았고, 뿌리에서 P, Ca, Mg, Na, Fe, Mn, Zn, Cu의 순으로, 씨에서는 P, Na, Ca, Mg, Mn, Fe, Cu, Zn의 순으로 함량이 많았다. 뿌리에서 vitamin C의 함량은 5.72 mg/100 g으로 가장 많았고 씨에서는 6.05 mg/100 g으로 나타났다. 비타민의 함량을 살펴 본 결과 녹차 뿌리보다 씨에 더욱 많은 종류의 vitamin이 함유되어 있었고 그 함량도 뿌리에 비해 다소 높은 경향이었다. 총 아미노산은 뿌리에 1,651 mg/100 g, 씨에 4,335 mg/100 g 함유되어 있어 각국 시판 녹차에 비해 그 함량이 높았고, 녹차에서 존재하지 않는 아미노산인 phenylalanine도 16.00 mg/100 g와 139.00 mg/100 g 존재하는 것으로 나타났다. 유리 아미노산의 경우, 뿌리에서 녹차의 품질을 평가하는 theanine이 전체 유리 아미노산 함량의 48%를 차지하였고 쓴맛을 내는 arginine, valine 및 tryptophan 등의 함량이 높았으며, 열매에서는 단맛과 감칠맛을 내는 glutamic acid, alanine, theanine, aspartic acid 및 serine 등의 함량이 많았다. 총 polyphenol은 뿌리에 237 mg/100 g으로 씨에서의 함량 81 mg/100 g보다 3배가량 높았다. Polyphenol 중 catechin의 함량은 뿌리에서 66 mg/100 g, 씨에서 1 mg/100 g으로 나타났고 caffeic acid는 뿌리에서 0.78 mg/100 g으로 나타났지만 씨에서는 검출되지 않았다. 또한 chlorogenic acid는 뿌리와 씨에서 각각 3.7 mg/100 g과 6.6 mg/100 g 검출되었다.

• 대한본초학회지
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• 제26권2호
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• pp.67-73
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• 2011

Objectives : The purpose of this research was total polyphenol contents, anti-oxidant activities, anti-inflammatory activities and anti-wrinkle activities of Ulleung islands plants for application as a cosmeceutical ingredients Methods : We were experimented total polyphenol contents, anti-oxidant activities, anti-inflammatory activity and anti-wrinkle activities. Results : In the physiological activities, most Ulleung islands plants is showed the highest anti-oxidant, anti-inflammatory activity in ABTS+ radical cation scavenging activity, hydrogen peroxide($H_2O_2$) scavenging activity, nitric oxide scavenging activity. All experiment of the water and ethanol extract from the Ulleung islands plants ingredients were gradually increased as well. Conclusions : These results suggest that extracts from Ulleung islands plants can be used in natural ingredient in food or cosmetic industry.

• 생약학회지
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• 제48권3호
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• pp.208-212
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• 2017

In this study, analysis of active compounds that are believed to be highly relevant to antioxidant activity was carried out on the methanol extract and its solvent fractions of Corni fructus. The DPPH radical scavenging activity for the comparison of antioxidant activity was higher in order of aqueous fraction > methanol extract > ethyl acetate fraction > n-hexane fraction. It is similar to the order of total polyphenol contents in the samples. As a result of LC-MS analysis, phenolic acid compounds such as caffeic acid, gallic acid and chlorogenic acid and lognin, which is known as a representative active ingredient of Corni fructus, were identified as active compounds. And the antioxidative activity and the total polyphenol content of the extracts and solvent fractions were found to be related to the contents of the compounds. Particularly, it was confirmed that phenolic acid such as caffeic acid contributes to the antioxidative activity of the aqueous fraction of Corni fructus methanol extract.

• 한국식품조리과학회지
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• 제20권2호
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• pp.219-226
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• 2004

This study was performed to investigate the change of chemical components and antioxidant activities in Hizikia fusiformis(Harvey) OKAMURA with blanching times. For chemical composition, the contents of minerals (K, Ca, Na, Mg, Fe, Cu, Mn and Zn), vitamins (vitamin C, ${\beta}$-carotene and ${\alpha}$-tocopherol) and total polyphenol were analyzed. The antioxidant activity was determined through free radicals (DPPH radical, superoxide anion radical, hydroxyl radical and hydrogen peroxide) scavenging activity and linoleic acid peroxidation inhibitory activity. As the blanching time increased, the contents of all minerals, vitamin C and total polyphenol decreased. However ${\beta}$-carotene and ${\beta}$-tocopherol were not affected by the blanching time. In addition antioxidant activities decreased with increasing blanching time.

• 한국식생활문화학회지
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• 제19권4호
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• pp.369-377
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• 2004

This study was performed to investigate the change of biologically functional compounds and antioxidant activities in Ecklonia cava with blanching times. As biologically functional compounds, the contents of minerals(K, Ca, Na, Mg, Fe, Cu, Mn and Zn), vitamins($vitamin\;C,\;{\beta}-carotene\;and\;{\alpha}-tocopherol$) and total polyphenol were analyzed. And antioxidant activity was determined through free radicals(DPPH radical, superoxide anion radical, hydroxyl radical and hydrogen peroxide) scavenging activity and linoleic acid peroxidation inhibitory activity. As the blanching time increased, the contents of minerals, vitamin C, ${\beta}-carotene$ and total polyphenol were decreased however ${\alpha}-tocopherol$ was not affected by blanching time, and antioxidant activities were decreased with blanching time.

• 한국식품과학회지
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• 제32권5호
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• pp.1022-1028
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• 2000

마이크로웨이브 추출방법과 환류냉각 추출방법을 비교한 결과, 물과 메탄올 및 에탄올 혼합용매로 추출한 경우 마이크로웨이브 추출 방법에 의하여 추출시간을 단축시키면서 환류냉각 추출방법에서와 같은 수준의 추출율과 총 폴리페놀 함량을 갖는 섬쑥부쟁이 추출물을 얻을 수 있었다. 마이크로웨이브 추출시 최적 마이크로웨이브 에너지는 $120{\sim}150$ W였고 추출시간의 경우 $4{\sim}8$분이 적당하였다. 추출에 사용한 용매들 가운데 에탄올, 메탄올보다 물 그리고 물과 에탄올 또는 메탄올 혼합용매를 사용한 추출물의 추출율, 총 폴리페놀 함량 및 전자공여 효과가 높은 것으로 나타났다.

• Preventive Nutrition and Food Science
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• 제14권2호
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• pp.109-115
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• 2009

The principal objective of this study was to assess the radical scavenging activities and total polyphenols, flavonoids, and flavonol contents of takju, a Korean traditional rice wine. The antioxidant properties of the wine and takju samples were evaluated using five distinct assays: specifically, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-di-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS); hydroxyl; superoxide anion; and nitric oxide (NO) radical-scavenging activity assays. In this study, the takju evidenced strong scavenging activities against the hydroxyl, superoxide anion, and NO radicals. Furthermore, the total polyphenol contents of T-2 were similar to those previously observed in white wine (p<0.05). However, the flavonoids and flavonol contents of all takju samples were significantly (p<0.05) lower than that of white wine. The results of this study show that takju possesses a powerful radical scavenging activity against a variety of oxidative systems. The findings of this study also indicate that takju should be considered a useful antioxidant, and that their functional compound reduces oxidative stress.

• 한국지역사회생활과학회:학술대회논문집
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• 한국지역사회생활과학회 2009년도 추계학술대회
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• pp.90-90
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• 2009

Cereals or cereal grains, are grasses cultivated for the edible components of their fruit seeds. In this study we evaluated the chemical characteristics of 12 raw cereals produced in Korea. The nutritional and functional contents such as moisture, carbohydrate, crude protein, crude lipid, ash, dietary fiber, minerals(Ca, P, Fe, Na, and K), vitamins (A, E, C, B groups, folic acid, and niacin), total polyphenol, carotenoid, and flavonoid containing isoflavone were investigated. Especially, the dietary fiber were determined and divided into three contents such as total, soluble, and insoluble fibers. As a results, the black rice was shown the high total polyphenol and flavonoid contents. They were included the rich vitamins, minerals, carbohydrates, fats and protein. The other chemical compositions were not shown the remarkable differences among cereal cultivar. This result can be used as basic data for specific cooking or functional study.