• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.277-277
• /
• 2022

Lignin modification has been a breeding target for the improvements of forage digestibility and fermentation in whole crop silage(WCS) rice. In rice, gold hull and internode 2 (gh2) was identified as a lignin-deficient mutant. gh2 exhibits a reddish-brown pigmentation in the hull and the internode is located on the short arm of chromosome 2 and codes for cinnamyl-alcohol dehydrogenase (CAD). To develop WCS rice variety improved digestibility and fermentation, we measured acid detergent fiber (ADF), lignin and total digestible nutrient (TDN) calculated from ADF (TDN=88.9-(0.79% × ADF) and performed marker-assisted selection using CAD(Os2g0187800) gene first intron region specific marker with 55 Jungmo1038/J.collection lines. Those lines had lignin content range from 0.82 to 6.61%, ADF from 15.8 to 45.8%, TDN from 52.7 to 78.8 compared to 'Jungmo1038'(1.53,20.7,72.6), 'J.collection'(0.98,12.8,78.8%) and gh2 were introgressed into 44 lines. Considering on these genotype and low-lignin phenotype, we finally selected 2 elite lines(Suweon668, Suweon669). Suweon668 and Suweon669 line are high biomass-low lignin lines that the ADF content is relatively low, even though the dry matter weight is high. Also they have lodging and shattering resistance and glabrous leaf and hull important to improve cattle palatability. Our results will provide that rice can be improved for forage digestibility and fermentation with low lignin concentration.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.43 no.3
• /
• pp.162-167
• /
• 2023

The experiment aim to breed an early-maturing variety of Italian ryegrass (Lolium multiflorum Lam.). It was conducted at the Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan, Korea, from 2022 to 2023. The new variety named "Oasis" is a diploid with green leaves and has a semi-erect growth habit in late fall, and an erect growth habit in early spring. With a heading date of May 10, Oasis is categorized as an early-maturing variety. Compared with the "Florida80" as a control variety, Oasis's flag leaf was 1 mm wider and 2 cm shorter, while the plant length was 7.8 cm longer. Additionally, the ear of Oasis was 1.2 cm longer than that of Florida80, and it showed lodging resistance. The dry matter yield of Oasis (8,805 kg/ha) was higher than that of Florida80. The total digestible nutrient and crude protein contents of Oasis were 64.5%, and 9.3%, respectively, which were 1.1% and 0.6% higher than those of Florida80, respectively. The neutral and acid detergent fiber contents in Oasis were 54.2% and 30.8%, respectively, which was 1.9% and 1.4% lower than those of Florida80, respectively. These results indicate that Oasis has good dry matter yield in the most of region and especially, middle and northern region of South Korea.

• Korean Journal of Organic Agriculture
• /
• v.19 no.3
• /
• pp.373-384
• /
• 2011

This experiment was conducted to investigate the selection of regional double cropping system for production of organic forage in middle part of Korea and investigated their productivity, feed values and stock carrying capacity. The test results proved the Sorghum${\times}$Sudangrass hybrid was 10.9 $ton{\cdot}ha^{-1}$, the one of Rye + Red clover and Rye + Hairy vetch were the highest one as 3.3 $ton{\cdot}ha^{-1}$ and the relative yield about a Rye is higher in the dry matter yield in the field soil. And as for the relative forage values (RFV), Corn is the most excellent ones as 96.6 but there were no the significant difference between the Sorghum${\times}$Sudangrass hybrid of 84.4 and it. The total digestible nutrient (TDN) content in Rye + Hairy vetch is higher than others as 59.7%. As for the relative feed value, Rye + Red clover is the highest one as 83.8 but there was no significant differences between them. As for the ability to raise livestock in the field soil, the average values of $K_{CP}$ and $K_{TDN}$ are shown as 4.27 head/ha/yr in Sorghum${\times}$Sudangrass hybrid. As for the same crops, the average values of $K_{CP}$ and $K_{TDN}$ showed the highest ones as 1.74 head/ha/yr, in Rye + Red clover but there were no the significant differences. As for the dry matter yield of the rice field soil, organic rice straw was 3.3 $ton{\cdot}ha^{-1}$ and Rye + Red clover significantly showed the highest values as 4.1 $ton{\cdot}ha^{-1}$. In the rice filed soil, the crude protein content of organic rice straw was 3.1% and the TDN content of organic rice straw was 55%. The TDN content in Rye + Red clover was higher than others as 59.7%. As for the relative forage values, Rye + Red clover showed the highest one as 83.8 but there were no significant differences between them. As for the ability to raise livestock in the rice field, the average values of $K_{CP}$ and $K_{TDN}$ in the organic rice straw was 1.04 head/ha/yr and the average values of $K_{CP}$ and $K_{TDN}$ in Rye + Red clover is the highest one as 1.84 head/ha/yr, but there were no significant differences in all of them.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.92-92
• /
• 2022

In preparation for the ever-changing climate and unification of North Korea and South Korea, it is necessary to increase the grain self-sufficiency rate by selecting crops with good utilization in high-altitude regions. The principle is to sow pastures at the end of August. However, sowing occurs in spring because the sowing period is missed when the weather is bad or when the workforce is insufficient. Sometimes when the grassland is completely devastated, it is frequently sowed in spring. In addition, North Korea consists of a high-altitude regions, and has been devastated in a general mountainous region. As a result, the landscape is not good and it is vulnerable to natural disasters such as landslides. Therefore, to prevent this, pasture must be sowed in the high-altitude regions. The goal of this study was to evaluate and compare yield and feed value of pasture species and varieties by spring sowing in high-latitude regions. The study was conducted in Pyeongchang, Gangwon-do, which is 700m height above sea level. The pasture species and varieties was sown on April 24, 2022. Each treatment was carried out by sowing 30 kg/ha, the experiment field size was 1 m²(1m×1m), and randomized block design with tri-repeat. The total of 14 varieties was used in the study, 6 varieties of Orchardgrass (OG), 6 varieties of Tall fescue (TF) and 2 varieties of Perennial ryegrass (PRG). The grassland composition fertilization using (N:P₂O₅:K₂O at 80:200:70 kg/ha) was conducted and management fertilizer was N:P₂O₅:K₂O at 210:150:180 kg/ha. The first harvest was June 26,2022 and the second harvest was on August 16, 2022. For statistical analysis of the data, an Analysis of Variance (ANOVA) was performed using the R3.6.3 software program, and all data was subjected to analysis using Duncan's multiple range test. Significance was set at the 5% level. The dry matter yield at the first harvest was the highest in PRG, and second harvest was the highest in TF (p < 0.05). Overall, PRG showed a trend of gradually decreasing growth, OG and TF showed a trend of gradually improving growth. This showed that PRG was considerably weaker to summer depression than other pasture species. Comparing the total dry matter yield, TF was the highest (4,565.45 kg/ha), but there was no significance difference with PRG (4,487.24 kg/ha) (p < 0.05). In addition, comparing the total TDN (total digestible nutrient) yield, TF was the highest (3147.33 kg/ha), second in PRG (2975.67 kg/ha) and third in OG (2052.33 kg/ha). Since this result is the data of the second harvests, if the result is derived by the end of next year, it will be provided as basic data for selection of pasture species and varieties suitable for spring seeding in high-altitude regions.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.44 no.2
• /
• pp.92-98
• /
• 2024

The objective of this study was to investigate the comparison of feeding a total mixed ration (TMR) containing imported alfalfa hay and TMR containing hot-air dried domestic alfalfa on rumination activity, milk production, and composition in lactating dairy cows. Ten Holstein dairy cows were divided into two groups: control (imported alfalfa hay + TMR) and treatment (hot-air dried domestic alfalfa + TMR) groups. The study was conducted over a total period of 18 days, including a 13-days adaptation period and a 5-days main experimental period. The results indicated no differences in total digestible nutrient and net energy intake between the imported and hot-air dried alfalfa. Body weight was not significantly different between the two groups (p>0.05), however, total feed intake and rumination time were significantly and tendentially higher in the treatment group compared with control group, respectively (p<0.001; p=0.075). Milk yield was not significantly different between the two groups (p>0.05), however, milk fat (kg) and lactose (%) concentration were significantly higher in the treatment group compared with control group (p=0.016; p=0.02). This study confirms that feeding TMR with hot-air dried domestic alfalfa results in no differences of feed intake, rumination activity, and milk productivity. Therefore, it is considered that hot-air dried domestic alfalfa can be used as a substitute for imported alfalfa on lactating dairy cows.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.1
• /
• pp.31-38
• /
• 2000

This study was conducted to evaluate changes in the lysine to digestible energy (DE) ratio on performance, apparent ileal and fecal nutrient digestibilities as well as blood urea nitrogen (BUN), and to estimate optimal lysine:DE ratios for growing pigs of different sexes. A total of 150 pigs ($(Landrace{\times}Yorkshire){\times}Duroc$, 16.78 kg average body weight, 75 barrows and 75 gilts) was randomly allotted into a $2{\times}3$ (sex by diet) factorial design. Three diets were formulated to contain a crude protein level of 19%, a DE level of 3.5 Mcal/kg with three lysine:DE ratios of 3.2 (low), 3.5 (middle) and 3.8 (high) g lysine/Mcal DE per kg diet for both barrows and gilts throughout the study. With increasing dietary lysine:DE ratio, the average daily gain (ADG) of barrows decreased but there was no significant difference among treatments (p>0.05). However, ADG was significantly higher in gilts fed the diet containing the high lysine:DE ratio (p<0.05), followed by the middle and low lysine:DE ratio dietary groups. No significant effects of lysine:DE ratios on feed intake (ADFI) and feed conversion (F/G) were observed for barrows and gilts during overall period (p>0.05), while the optimal F/G was found in barrows fed diets of low and in gilts fed high lysine:DE ratio. Blood urea nitrogen had a positive relationship with growth rate. The results showed that the optimal lysine:DE ratios were 3.2 and 3.8 g lysine/Mcal DE per kg diet for barrows and gilts of 16 to 57 kg body weight, respectively.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.28 no.1
• /
• pp.29-34
• /
• 2008

This experiment was conducted to study the effects of growth stage and variety on the quality of whole crop rice(WCR) silage at National Institute of Animal Science, RDA from 2004 to 2005. Two types of rice("Chucheong" for food and "Hamasari" for whole crop) were harvested at six different growth stages (heading, flowering, milk, dough, yellow ripen and fully ripen stage) and ensiled each harvest stages. Crude protein(CP) content of all whole crop rice silage was also decreased with progressed maturity at harvest and TDN(total digestible nutrient) content of WCR was increased. The average CP content of whole crop rice was higher than that of food rice. The contents of ADF(acid detergent fiber) and NDF(neutral detergent fiber) decreased with delayed harvest maturity. The content of TDN estimated from ADF content was increased with progressed maturity, but there was not found significant difference between rice varieties(p<0.05). The average pH value was 4.69 and it was increased with delayed harvest maturity. Acetic and butyric acid content were decreased, but lactic acid content was increased with progressed maturity. The experiments presented here show that all rice varieties may give a good quality silage. But some wrong practical method(grain loss, productivity, nutrient value, etc.) will make poor quality of rice silage. Therefore, dough stage of harvest maturity will be recommendable as proper harvest time for making high quality of whole crop rice silage in Korea.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.8
• /
• pp.1202-1208
• /
• 2009

Synbiotics is the term used for a mixture of probiotics (live microbial feed additives that beneficially affects the host animal) and prebiotics (non-digestible food ingredients that beneficially affect the organism). This study investigated the effect of probiotics from anaerobic microflora with prebiotics on growth performance, nutrient digestibility, noxious gas emission and fecal microbial population in weaning pigs. 150 pigs with an initial BW of 6.80${\pm}$0.32 kg (20 d of age) were randomly assigned to 5 dietary treatments as follows: i) US, basal diet+0.15% antibiotics (0.05% oxytetracycline 200 and 0.10% tiamulin 38 g), ii) BS, basal diet+0.2% synbiotics (probiotics from bacteria), iii) YS, basal diet+0.2% synbiotics (probiotics from yeast), iv) MS, basal diet+0.2% synbiotics (probiotics from mold), v) CS, basal diet+0.2% synbiotics (from compounds of bacteria, yeast and mold). The probiotics were contained in $10^{9}$ cfu/ml, $10^{5}$ cfu/ml and $10^{3}$ tfu/ml of bacteria, yeast and molds, respectively. The same prebiotics (mannan oligosaccharide, lactose, sodium acetate and ammonium citrate) was used for all the synbiotics. Pigs were housed individually for a 16-day experimental period. Growth performance showed no significant difference between antibiotic treatments and synbiotics-added treatments. The BS treatment showed higher (p<0.05) dry matter (DM) and nitrogen digestibility while ether extract and crude fiber digestibility were not affected by the dietary treatment. Also, the BS treatment decreased (p<0.05) fecal ammonia and amine gas emissions. Hydrogen sulfide concentration was also decreased (p<0.05) in BS, YS and MS treatments compared to other treatments. Moreover, all the synbioticsadded treatments increased fecal acetic acid concentration while the CS treatment had lower propionic acid concentration than the US treatment (p<0.05) gas emissions but decreased in fecal propionate gas emissions. Total fecal bacteria and Escherichia coli populations did not differ significantly among the treatments, while the Shigella counts were decreased (p<0.05) in synbiotics-included treatment. Fecal bacteria population was higher in the YS treatment than other treatments (p<0.05). The BS treatment had higher yeast concentration than YS, MS and CS treatments, while US treatment had higher mold concentrations than MS treatment (p<0.05). Therefore, the results of the present study suggest that synbiotics are as effective as antibiotics on growth performance, nutrient digestibility and fecal microflora composition in weaning pigs. Additionally, synbiotics from anaerobic microflora can decrease fecal noxious gas emission and synbiotics can substitute for antibiotics in weaning pigs.

• Asian-Australasian Journal of Animal Sciences
• /
• v.33 no.2
• /
• pp.286-293
• /
• 2020

Objective: Two experiments were conducted to evaluate the effects of corn particle size and lipid form on the apparent total tract digestibility (ATTD) of energy and nutrients in diets for growing pigs. Methods: In Exp. 1, thirty barrows (initial body weight [BW], 53.1±3.9 kg) were allotted to 1 of 5 diets formulated with 96.9% corn ground to 441, 543, 618, 659, and 768 ㎛, respectively. In Exp. 2, thirty-six barrows (initial BW, 54.7±3.6 kg) were allotted to 1 of 6 diets formulated by including 2% or 15% corn germ (CG 2 or CG 15), 1% or 6% corn oil (CO 1 or CO 6), 1% CO+2% corn germ meal (CO 1+CGM 2), or 6% CO+15% corn germ meal (CO 6+CGM 15), respectively. Results: The ATTD of gross energy (GE) and the digestible energy (DE) in diet and corn grain linearly decreased as the corn particle size increased (p<0.05) from 441 to 768 ㎛. Particle size had a quadratic effect (p<0.05) on the ATTD of neutral detergent fiber and acid detergent fiber in diets, and which firstly increased and then decreased as the corn particle size increased from 441 to 618 ㎛ and 618 to 768 ㎛, respectively. The ATTD of GE, ether extract (EE), and the DE in CO 1 diet and CO 6 diet was greater (p<0.05) than that in CG 2 diet and CG 15 diet, respectively. The ATTD of EE in CO 6 diet and CO 6+CGM 15 diet was greater (p<0.05) than that in CO 1 diet and CO 1+CGM 2 diet. Conclusion: Less than 618 ㎛ was recommended for corn particle size in growing pig's diet and extracted lipid had greater digestibility than the intact lipid in corn. Higher concentration of extracted CO had greater digestibility of EE compared with lower concentrations of CO diet.

• Asian-Australasian Journal of Animal Sciences
• /
• v.33 no.11
• /
• pp.1809-1816
• /
• 2020

Objective: This study was conducted to evaluate the effects of inclusion level and amino acid (AA) supplementation on energy values of soybean oil (SO) as determined by difference method or regression method when fed to growing pigs. Methods: Thirty-six barrows (initial body weight: 28.0±1.3 kg) were randomly assigned to one of 6 dietary treatments, which included 2 control diets formulated using a basal diet with or without AA supplementation, and 4 experimental diets with 5% or 10% SO addition in the 2 control diets, respectively. All pigs were individually housed in metabolism crates for 19 d, and during the last 5 d, total urine and feces production were collected. The nutrient digestibility in diets and the digestible energy (DE) and metabolizable energy (ME) values of SO were determined using the difference method and the regression method, respectively. Results: Our results showed that there were no interaction effects (p>0.05) between AA supplementation and SO inclusion levels on energy values of SO and dietary nutrient digestibility. The DE and ME values of SO determined by the difference method were not affected (p>0.05) by AA supplementation, however, the ME value of SO increased (p<0.05) as the inclusion level of SO increased. Moreover, the energy values of SO determined using the regression method were close to those determined using difference method with 10% SO inclusion, but were greater than those obtained using difference method with 5% SO inclusion. Conclusion: We concluded that the DE and ME values of SO increased with the inclusion level but were not affected by AA supplementation in the range of 0% to 10%. The difference method can substitute for the regression method to determine the DE and ME values of SO when the inclusion level is 10%, but not at 5% inclusion level.