• 한국CDE학회논문집
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• 제4권2호
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• pp.110-120
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• 1999

In general, turbine blades are usually machined on 5-axis NC machine. The 5-axis machining of sculptured surface offers many advantages over 3-axis machining including the faster material-removal rates and an improved surface finish. But it is difficult and time-consuming to generated interference-free 5-axis tool path. This paper describes research on the algorithm for generation of an interference-free 5-axis NC data for machining turbine blades. The approach, using the section profile derived from the intersection of cutting planes with a triangulated-surface approximation, includes (1) CL-data generation by detecting an interference-free heel angle (2) the calculation method for finding a adaptive feed-rate value, and (3) the inverse kinematics depending on the structure of 5-axis machine.

• Animal cells and systems
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• 제11권2호
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• pp.99-104
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• 2007

RNA interference (RNAi) is the phenomenon of gene silencing by double-stranded RNA (dsRNA) at transcriptional and post-transcriptional levels in a sequence-specific manner. Reverse genetic approaches using RNA interference (RNAi) have become a major tool for biological researches since its discovery in the nematode Caenorhabditis elegans. In this review, we overview how the RNAi phenomenon was discovered and how the underlying mechanism has been elucidated. We also describe and discuss how RNAi experiments can be performed and how RNAi can be used for genetic studies.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.273-276
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• 2006

RNA interference (RNAi), a process of sequence-specific gene suppression, has been known as a natural gene regulatory mechanism in a wide range of lower organisms. Recently, we have reported that a transfection of human U6 promoter (hU6) driven hairpin small-interference RNA (siRNA) plasmid specifically knocks down the target gene by post-transcriptional gene silencing in mammalian cells. Here we report that transfection of polymerase chain reaction (PCR) products, containing human U6 promoter with hairpin siRNA, knocks down the target gene expression in human teratocarcinoma NT-2/D1 cells. Moreover, we showed 3' end termination sequence, 5 Ts, is not critical elements for knocking down in PCR-based siRNA system. Therefore, the PCR-based siRNA system is a promising tool not only for the screening but also to temporally regulate gene expression in the human progenitor cells.

• International Journal of Aerospace System Engineering
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• 제10권1호
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• pp.1-13
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• 2023

The present study was attempted to investigate flow interference effects and the aerodynamic characteristics of the front and rear wings of a joined-wing aircraft by changing the configuration variables. The study was performed using a computational fluid dynamics(CFD) tool to demonstrate forward flight and analyze aerodynamic characteristics. A total of 9 configurations were analyzed with variations on the position, height, dihedral angle, incidence angle, twist angle, sweepback angle, and wing area ratio of the front and rear wings while the fuselage was fixed. The quantities of aerodynamic coefficients were confirmed in accordance with joined-wing configurations. The closer the front and rear wings were located, the greater the flow interference effects tended. Interestingly, the rear wing did not any configuration change, the lift coefficient of the rear wing was decreased when adjusted to increase the incidence angle of the front wing. The phenomenon was appeared due to an effective angle of attack alteration of the rear wing resulting from the flow interference by the front wing configurations.

• 한국인터넷방송통신학회논문지
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• 제10권3호
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• pp.83-89
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• 2010

본 논문은 간섭원으로써 4.5 GHz를 사용하는 UWB 센서가 동대역을 사용하는 피간섭원 광대역 무선 시스템에 미치는 간섭을 분석하였다. 분석방법으로는 Minimum Coupling Loss(MCL)와 Spectrum Engineering Analysis Monte Carlo Advanced Tool(SEAMCAT)을 사용하였으며 그 결과는 다음과 같다. 양립성 확보를 위해서 UWB 센서와 광대역 무선 시스템의 이동국 사이에는 1.2 m 이상의 보호 이격거리가 요구되었고 다수의 UWB 센서가 존재하는 경우에는 간섭 확률 5 % 이하를 만족하기 위해 UWB 수신기 PSD는 -68.5 dBm/MHz 이하로 요구되었다.

• 한국정밀공학회지
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• 제10권3호
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• pp.161-167
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• 1993

This paper deals with a new method for the machining of a die cavity defined by sculptured surfaces. In machining die cavities or pockets, process planners have been faced with some troulbes. One of the troubles ius to rebove a great deal of material within a given boundary while avoiding cutter interference. Cutter interference is a ciritical problem in NC machining of a die cavity. Even though this cavity machining has been implemented in many CAM systems, most of them can handle limited shapes of cavities or pockets. In this paper, a procedure has been developed to machine die cavity or pocket of a sculptured surface. The offset surfaces of the part surfaces and boundary surfaces are determined to calculate the intersection curves. These intersection curves form a FACE on the part surface, and the interference free tool pathe is generated by eliminating the points outside the FACE when computing the cutter contact (CC) data. Additionally, the cutter location (CL) data obtained from the CC data convert to an NC data. The NC data generated through this algorithm are verified on the CINCINNATI milling machine. A propotype die cavity machining system has been implemented in FORTRAN language and FIGARO graphics library on IRIS workstation.

• 한국CDE학회논문집
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• 제13권5호
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• pp.382-390
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• 2008

Five-axis machining has been applied to manufacture of turbine blades, impellers, marine propellers. Nowadays it extends to mold & die machining, where more productivity as well as added value is expected. The five-axis machining can be divided into positional and continuous, according to the variableness of tool orientation during material removal process. The positional five-axis machining is commonly applied to the regional machining on a whole part surface in mold manufacturing industry, where the tool orientation for each region (area) should be determined to be feasible, that is, avoiding any interference such as machine tool collision, etc. Therefore it is required for a CAM programmer to decide a feasible tool orientation in generating tool-paths on a designated area, because it is a very tedious job to obtain such information by utilizing a commercial CAM system. The developed system generates feasibility data on tool orientation and machining region, which facilitates the CAM programmer's decision on a feasible tool orientation.

• 가정간호학회지
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• 제24권2호
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• pp.189-199
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• 2017

Purpose: This study aimed to investigate the effects of foot bath therapy on the symptom intensity, distress, and interference with usual activities due to chemotherapy-induced peripheral neuropathy (CIPN) in patients with metastatic and recurrent cancer. Methods: Foot bath therapy was administered to the experimental group for >8 sessions in 2 weeks, and the chemotherapy-induced peripheral neuropathy assessment tool (CIPNAT) was used to measure its effects on the symptom intensity, distress, and interference with usual activities due to CIPN. SPSS was used to perform data analyses including descriptive statistics, chi-square test, Fisher's exact test, t-test, paired t-test, and repeated measures ANCOVA. Results: A statistically significant difference in the variation of the symptom intensity, distress, and interference with usual activities due to CIPN was observed between the two groups; however, a statistically insignificant difference was observed between the groups and time of interaction. Conclusion: Foot bath therapy can be used as a simple and effective clinical or home care nursing intervention to improve the symptom intensity, distress and interference with usual activities due to CIPN.

• Design & Manufacturing
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• 제6권1호
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• pp.45-51
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• 2012

The main goal of this paper is to establish an interference tolerance for determining optimal amount of clearance in the shaft-bearing system supported by radial ball bearings. The 2-D frictional contact model was employed for the FE analysis between the shaft and the inner raceway. Several examples were simulated using different material properties for the solid shaft. Efforts were focused on the deformation applied in the radial direction to select suitable bearings. The analysis results showed that the initial axial preload applied on the bearings plays a significant role to reduce bearing fatigue life. The proposed design parameters obtained by numerical simulations can approximately predict a rate of bearing life reduction as a function of shaft diameter ratio. This analysis can also be used to calculate the optimal initial radial clearance in order to obtain a shaft-bearing system design for high accuracy and long life.

• 한국경영과학회:학술대회논문집
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• 대한산업공학회/한국경영과학회 2000년도 춘계공동학술대회 논문집
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• pp.389-392
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• 2000

Gouge-free tool-path generation is an important issue in mold & die machining and researches on cutter interference avoidance can be found in many articles. One of the various methods is construction of tool-offlet surface or cutter-location (CL) surface on which the cutter-center point (CL-point) locates. Provided that the CL surface is represented in a suitable form, cutter-interference avoidance can be performed without the burden of computing CL data for every cutter-contact (CC) point. In the paper, various methods of constructing a CL surface in the z-map form are presented, where z-map is a special form of discrete nonparametric representation in which the height values at grid points on the xy-plane are stored as a 2D array z［i,j］.