• Korean Journal of Veterinary Service
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• v.38 no.1
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• pp.25-30
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• 2015

Bee venom has been used as to prevent and treat bovine mastitis as natural antimicrobial compounds in some dairy cattle farms in Korea. It is needed to determine the residual of bee venom in milks of dairy cattle treated with bee venom. Since bee venom is not approved as a raw material for animal drugs, the preprocessing method to detect bee venom residual in milk and the tolerance limit for its residue has not been established yet in Korea. Therefore, the purpose of this study was to develop pre-processing method not affecting major component of bee venom for detection of its residue in milks using ultra-high performance liauid chromatography (UHPLC). In addition, bee venom residue was also analyzed in milk samples of dairy cattle treated for mastitis with bee venom using UHPLC with the developed pre-processing method in this study. As a result, melittin, histamin and phospolipase A2, the major components of bee venom, were all detected by UHPLC with the pre-processing method developed in this study. The results of this study suggest that the pre-processing method developed in this study can be useful to detect bee venom residue in dairy cattle milk. We also found that no bee venom residues were detected in milk samples collected from dairy cattle treated with bee venom after 1 and 3 days, respectively.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.19 no.2
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• pp.104-109
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• 2016

Purpose: Regurgitation is a common physiological phenomenon in infants. The aim of the present study was to evaluate the efficacy of a new anti-regurgitation (AR) formula (Novalac), thickened with an innovative complex including fibres, on the daily number of regurgitations and to assess its impact on stool consistency and frequency. Methods: Infants younger than five months, presenting at least 5 regurgitations per day were recruited in this trial. The efficacy of the new formula on regurgitation (daily number and Vandenplas score), stool frequency and consistency were assessed at day 14 and 90. Growth data were recorded at each study visit. Results: Ninety babies (mean age $9.6{\pm}5.8weeks$) were included in the full analysis data set. The mean number of regurgitation episodes at inclusion was $7.3{\pm}3.4$. In all infants, regurgitations improved after 2 weeks. The daily number of regurgitations decreased significantly ($-6.3{\pm}3.3$, p<0.001) including in those previously fed a thickened formula ($-6.2{\pm}3.0$, p<0.001). There was no significant change in stool consistency at day 14. After 3 months, 97.5% of infants had formed or soft stools. Growth was appropriate with a slight increase of weight-for-age z-score (from $-0.5{\pm}1.0$ to $-0.1{\pm}0.9$) and no change of weight-for length z-score ($-0.1{\pm}1.1$ to $-0.1{\pm}-1.1$). Conclusion: The new AR formula thickened with an innovative complex is very effective in reducing the daily number of regurgitations without having a negative impact on stools consistency.

• Journal of Korean Society on Water Environment
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• v.30 no.5
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• pp.549-558
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• 2014

Occurrence frequency of flood and drought tends to increase in last a few decades, leading to social and economic damage since the abnormality of climate changes is one of the causes for hydrologic facilities by exceedance its designed tolerance. Soil and Water Assessment Tool (SWAT) model was used in the study to estimate temporal variance of groundwater recharge and baseflow. It was limited to consider recession curve coefficients in SWAT model calibration process, thus the recession curve coefficient was estimated by the Baseflow Filter Program (BFLOW) before SWAT model calibration. Precipitation data were estimated for 2014 to 2100 using three models which are GFDL-ESM2G, IPSL-CM5A-LR, and MIROC-ESM with Representative Concentration Pathways (RCP) scenario. SWAT model was calibrated for the Soyang watershed with NSE of 0.83, and $R^2$ of 0.89. The percentage to precipitation of groundwater recharge and baseflow were 27.6% and 17.1% respectively in 2009. Streamflow, groundwater recharge, and baseflow were estimated to be increased with the estimated precipitation data. GFDL-ESM2g model provided the most large precipitation data in the 2025s, and IPSL-CM5A-LR provided the most large precipitation data in the 2055s and 2085s. Overall, groundwater recharge and baseflow displayed similar trend to the estimated precipitation data.

• Water for future
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• v.22 no.1
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• pp.81-90
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• 1989

A numerical simulation technique of three-dimensional finite difference model is developed to study the groundwater flow system in Dejima, an upland area which faces Kasumigaura Lake. For general perspectives of the groundwater flow system, a steady state three-dimensinal model is simulated. For the sedimentary mud formations which are found in the representative formation, three situations where the horizontal permeability is equal to 10 times and 100 times of the vertical one. The finite difference grid used in the simulation has 60$\times$50$\times$30=90,000 nodes. A converged solution with a tolerance of 0.001 meter of hydraulic head is set. Having determined the flow net by using a steady state three-dimensional model, the results for the three cases of hydraulic conductivity are compared with the results of the tracer methods (Bae and Kayane, 1987). With the aid of four representative vertical cross-sections, groundwater flow systems in the study area are assumed. The results of these are reasonally good comparable with the results of the basin yield and thd recharge-discharge distribution 8.

• Journal of Applied Biological Chemistry
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• v.60 no.4
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• pp.391-402
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• 2017

Lactic acid bacteria (LAB) are widely used as starter culture in food fermentation due to their harmless entity and health beneficial properties along with the ability to change texture, aroma, flavor and acidity of food products. In this study, five different LAB (FB003, FB058, FB077, FB081, and FB111) isolated from different Korean traditional fermented foods, assigned to Lactobacillus plantarum, Pediococcus pentosaceus, Weissella viridescens, Lactobacillus sakei, and Leuconostoc mesenteroides, respectively, on the basis of their physiological properties and 16S rRNA sequence analysis, to use as fermentation starter and check their ability to fasten the ripening time as well as the overall optimization in the fermentation condition. To check their suitability as starters, their safety, acid and bile tolerance, NaCl and temperature resistance, susceptibility to common antibiotics, and antimicrobial activities were determined. Squid jeotgal samples were prepared by adding $10^8CFU/g$ of each strain in different samples, which were then kept for fermentation at $4^{\circ}C$ and checked for their antioxidant activities at 0, 7, 15, and 21-day intervals. The samples fermented with FB003 and FB077 displayed the highest antioxidant activity. This study revealed two effective starter cultures (FB003, FB077) for squid jeotgal fermentation, which presented increased functionalities. The results of this study will lead to the development of novel industrial-scale production avenues for jeotgal preparation, and offer new insights into the prevention and control of chronic diseases.

• Journal of Korean Society for Geospatial Information Science
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• v.17 no.1
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• pp.97-104
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• 2009

Supplementary control point and urban control point have different purpose and characteristic in the view of installation but their measurement method and permanent marker position are very similar. Therefore if we could use them by connecting those two control point, we would improve the arrangement density as well as minimizing cost of restoration from the loss of control point. So we tried to suggest the possibility of connecting these two control point and usage by analysing positional accuracy of urban control point on the base of supplementary control point in this study. The result of this study is as following. When analysing the positional accuracy of 13 urban control points of the study areas, there were RMSE of connection errors between ${\pm}8cm$ and ${\pm}11cm$ for each measurement point. The result confirmed the possibility of connecting supplementary control point to urban control point and its usage within the allowable error tolerance that the present cadastral law permits.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.10A
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• pp.1546-1554
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• 1999

In this paper, Dynamic Fault Tree algorithm(DFT algorithm) is presented. This algorithm provides a concise representation of dynamic fault tolerance system including fault recovery techniques with fault detection, mask and switching function. And this algorithm define FDEP, CSP, SEQ, PAG gate which captures the dynamic characteristics of system. It show that this algorithm solved the constraints to satisfy the dynamic characteristics of system which there are in Markov and also this is able to satisfy the dynamic characteristics of system which there are in Markov and also this is able to covered the disadvantage of Fault tree methods. To show the key advantage of this algorithm, a traditional method, that is, Markov and Fault Tree, applies to TMR and Dual-Duplex systems with the dynamic characteristic and a presented method applies to those. He results proved that the DFT algorithm for solving the problems of the system is more effective than the Markov and Fault tree analysis model..

• Journal of the Korea Society for Simulation
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• v.17 no.4
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• pp.89-97
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• 2008

In wireless personal area networks (WPANs), the successful design of channel time allocation algorithm is a key factor in guaranteeing the various quality of service (QoS) requirements for the stringent real-time constraints of multimedia services. In this paper we propose a channel time allocation algorithm for achieving a high quality transmission and high channel error tolerance of MPEG stream in the IEEE 802.15.3 high-rate wireless PANs. Our algorithm exploits the characteristics of MPEG stream. When a new MPEG stream arrives, a DEV models it by the traffic envelope and delivers the traffic envelope to the piconet coordinator (PNC) along with the channel time request. The PNC performs channel time allocation according to the envelope. Performance of the proposed scheme is investigated by simulation and analysis. Our results show that compared to conventional scheme, the proposed scheme is very effective and provides a good performance under typical channel error conditions.

• Proceedings of the KSR Conference
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• 2008.06a
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• pp.1903-1908
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• 2008

This paper presents a characteristics of linear induction motor considering airgap variation for railway transit in order to achieve high performance of the vehicle. The operating principle of a LIM(Linear induction motor) is identical to a rotary induction motor. Space-time variant magnetic fields are generated by the primary part across the airgap and induce the electro-motive force(EMF) in the secondary part, a conducting sheet. This EMF generates the eddy currents, which interact with the airgap flux and so produce the thrust force known as Loren's force. Even though the operating principal is exactly same as a rotary motor, the linear motor has a finite length of the primary or secondary parts and it causes static and dynamic end-effect which is the discontinuous airgap flux phenomenon. This end-effect causes the deterioration of the system performance, especially in high-speed operation. Another problem is that construction tolerance restricts the minimum airgap in order to prevent a collision between the primary part and the secondary reaction plate. More over, as the airgap length is getting smaller, the attraction force between the primary part and secondary parts is getting larger dramatically and the attraction force would be another friction against propulsion. Therefore, it is necessary to figure out the characteristics of linear induction motor considering airgap variation in order to achieve high performance of the vehicle. The dynamic model of LIM taking into account end-effects is derived. Then the modified mechanical load equation considering the effect of the attraction and thrust force according to the airgap variation is analyzed. The simulation results are presented to show the effect of the LIM according to the airgap variation.

• Korean Journal of Agricultural Science
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• v.45 no.2
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• pp.248-257
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• 2018

Overexpression of AtCYP78A7, a gene encoding a cytochrome P450 protein, has been reported to improve tolerance to drought stress in genetically modified (GM) rice (Oryza sativa L.). The aim of this study was to evaluate the potential allergenicity and acute oral toxicity of the AtCYP78A7 protein expressed in GM rice. Bioinformatics analysis of the amino acid sequence of AtCYP78A7 did not identify any similarities with any known allergens or toxins. It showed that no known allergen had more than a 35% amino acid sequence homology with the AtCYP78A7 protein over an 80 amino acid window or more than 8 consecutive identical amino acids. The gene encoding the AtCYP78A7 protein was cloned in the pGEX-4T-1 vector and expressed in E. coli. Then, the AtCYP78A7 protein was purified and analyzed for acute oral toxicity. The AtCYP78A7 protein was fed at a dose of 2,000 mg/kg body weight in mice, and the changes in mortalities, clinical findings, and body weight were monitored for 14 days after the dosing. Necropsy was carried out on day 14. The protein did not cause any adverse effects when it was orally administered to mice at 2000 mg/kg body weight. These results indicate that the AtCYP78A7 protein expressed in GM rice would not be a potential allergen or toxin.