• Composites Research
• /
• 제23권1호
• /
• pp.8-16
• /
• 2010

섬유금속 적층판(FMLs)은 손상허용도를 향상시키고 무게를 줄이는데 적합하여 항공우주 응용의 신소재로 각광을 받고 있다. 본 연구에서는 우선 섬유와 알루미늄을 이용하여 적층판을 제조하여 인장시험을 수행 후 FMLs의 기계적 물성을 평가하였다. 또한 알루미늄과 섬유적층의 변화를 주어 낙추충격시험기(Drop Weight Impact Tester)를 이용하여 저속충격하에서 낙추 높이를 조절하여 각 종류의 시험편 마다 충격시간에 따른 하중과 충격흡수에너지를 각각 비교하였다. 추가로 유한요소해석을 이용하여 시험조건과 동일 조건하 인장과 충격거동해석을 수행한 결과를 실험치와 비교하여 실험과 이론해석이 잘 일치함을 보였다.

• 정보과학회 논문지
• /
• 제42권11호
• /
• pp.1303-1313
• /
• 2015

맵리듀스는 대용량 데이터 처리를 위한 시스템 안정성과 유용성을 제공한다. 맵리듀스 기반 k-최근접점 조인 질의처리 알고리즘은 두 데이터 집합 R과 S를 기반으로 R의 모든 레코드에 대해 S의 데이터 중 가장 인접한 k개의 레코드를 탐색하는 알고리즘으로써, 대용량 데이터 분석을 위한 중요한 질의 처리 알고리즘이다. 그러나 기존 k-최근접점 조인 질의처리 알고리즘은 높은 인덱스 구축비용 문제로 인해 대용량 데이터 처리에 적합하지 않은 문제점을 지닌다. 따라서, 본 논문에서는 그리드 기반 인덱스 생성 및 k-최근접점 질의 처리 알고리즘을 제안한다. 제안하는 기법은 질의 셀로부터 인접한 데이터만을 찾아 맵리듀스 테스크에 전송함으로써 데이터 전송 및 k-최근접점 연산 오버헤드를 줄인다. 성능평가를 통해, 제안하는 기법이 정확 매칭 질의를 제공하는 동시에 기존 기법에 비해 질의 처리 시간 측면에서 최대 7배의 성능을 개선함을 보인다.

• Journal of Plant Biotechnology
• /
• 제43권1호
• /
• pp.30-36
• /
• 2016

Brassinosteroids (BRs) are essential plant steroid hormones required for cell elongation, plant growth, development and abiotic and biotic stress tolerance. BRs are recognized by BRI1 receptor kinase that is localized in the plasma membrane, and the BRI1 protein will eventually autophosphorylate in the intracellular domain and transphosphorylate BAK1, which is a co-receptor in Arabidopsis thaliana. However, little is known of the role OsBRI1 receptor kinase plays in Oryza sativa, monocotyledonous plants, compared to that in Arabidopsis thaliana, dicotyledonous plants. As such, we have studied OsBRI1 receptor kinase in vitro and in vivo with recombinant protein and transgenic plants, whose phenotypes were also investigated. A OsBRI1 cytoplasmic domain (CD) recombinant protein was induced in BL21 (DE3) E.coli cells with IPTG, and purified to obtain OsBRI1 recombinant protein. Based on Western blot analysis with phospho-specific pTyr and pThr antibodies, OsBRI1 recombinant protein and OsBRI1-Flag protein were phosphorylated on Threonine residue(s), however, not on Tyrosine residue(s), both in vitro and in vivo. This is particularly intriguing as AtBRI1 protein was phosphorylated on both Ser/Thr and Tyr residues. Also, the OsBRI1 full-length gene was expressed in, and rescued, bri1-5 mutants, such as is seen in normal wild-type plants where AtBRI1-Flag rescues bri1-5 mutant plants. Root growth in seedlings decreased in Ws2, AtBRI1, and 3 independent OsBRI1 transgenic seedlings and had an almost complete lack of response to brassinolide in the bri1-5 mutant. In conclusion, OsBRI1, an orthologous gene of AtBRI1, can mediate normal BR signaling for plant growth and development in Arabidopsis thaliana.

• Journal of Plant Biotechnology
• /
• 제36권4호
• /
• pp.407-414
• /
• 2009

A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.

• 대한유전성대사질환학회지
• /
• 제15권3호
• /
• pp.118-126
• /
• 2015

Background and objectives: A deficiency of BH4 (tetrahydrobiopterin) not only causes the classical phenylketonuric phenotype, but also is the source of neurological signs and symptoms due to impaired syntheses of L-Dopa and serotonin. The treatment of BH4 deficiency usually consists of replacement with BH4 and the neurotransmitters. We performed this study to finding out long-term follow-up clinical symptoms and prognosis of BH4 deficiency. Methods: Clinical and biochemical, genetic analysis were done retrospectively from January 1999 to July 2015 in Soonchunhyang University Hospital. Results: In our study, total 207 patients were confirmed to hyperphenylalaninemia. Among them, 10 patients were BH4 deficiency. 9 patients were 6-pyruvoyl-tetrahydropterin (PTPS) deficiency and one patient was dihydropteridine reductase (DHPR) deficiency. The patients who received delayed treatment, most of our patients suffered from severe psychomotor retardation, hypotonia and seizure. c.259C>T mutation was identified most commonly in PTPS gene analysis. A patient with DHPR deficiency had a mental retardation, dystonia, seizure. His seizure semiology was dialeptic feature. His EEG showed generalized spike wave patterns. All patients had treated with tolerate L-Dopa, BH4 and 5-hydroxytryptophan. Most of the early treated patients have a good tolerance for drugs well. But some patients had neurologic symptoms, despite early detection and treatment. Conclusion: BH4 deficiency patients who had delayed treatment tend to have severe psychomotor problem and neurologic deficits.

• The Plant Pathology Journal
• /
• 제32권1호
• /
• pp.25-32
• /
• 2016

Potato is one of the most important crops worldwide. Its commercial cultivars are highly susceptible to many fungal and bacterial diseases. Among these, bacterial wilt caused by Ralstonia solanacearum causes significant yield loss. In the present study, integrated proteomics and genomics approaches were used in order to identify bacterial wilt resistant genes from Rs resistance potato cultivar CT-206-10. 2-DE and MALDI-TOF/TOF-MS analysis identified eight differentially abundant proteins including glycine-rich RNA binding protein (GRP), tomato stress induced-1 (TSI-1) protein, pathogenesis-related (STH-2) protein and pentatricopeptide repeat containing (PPR) protein in response to Rs infection. Further, semi-quantitative RT-PCR identified up-regulation in transcript levels of all these genes upon Rs infection. Taken together, our results showed the involvement of the identified proteins in the Rs stress tolerance in potato. In the future, it would be interesting to raise the transgenic plants to further validate their involvement in resistance against Rs in potato.

• Tribology and Lubricants
• /
• 제31권4호
• /
• pp.148-156
• /
• 2015

In the study, a design process for ensuring optimal clearance in a fuel injection pump(FIP) is suggested. Structure analysis and hydrodynamic lubrication analysis are performed to determine the optimal clearance. The FIP is simulated using Hypermesh, Abaqus 6.12 to evaluate the reduction of clearance when the maximum supply pressure is applied. The reduction in clearance is caused by the difference in the deformations between the barrel and plunger. When the deformation of the plunger is larger than that of the barrel, a reduction in clearance at the head part occurs. On the other hand, the maximum clearance reduction equals the maximum deformation in the stem part, because the deformation of barrel does not occur in this region. The clearance of FIP should be designed to be larger than maximum reduction of clearance in order to avoid contact between the plunger and barrel. In addition, the two-dimensional Reynolds equation is used to evaluate lubrication characteristics with variations of viscosity, clearance and nozzle for a laminar, incompressible, unsteady state flow. The equation is discretized using the finite difference method. The lubrication characteristics of FIP are investigated by comparing film parameter, which is the ratio of the minimum film thickness and surface roughness. The optimal clearance of FIP is to be designed by considering the maximum reduction in clearance, lubrication characteristics, machining limits and tolerance of clearance.

• Journal of Microbiology
• /
• 제44권4호
• /
• pp.369-374
• /
• 2006

To investigate the mechanism of salt tolerance of gram-positive moderately halophilic bacteria, two-dimensional gel electrophoresis (2-D PAGE) was employed to achieve high resolution maps of proteins of Halobacillus dabanensis $D-8^T$. Approximately 700 spots of proteins were identified from these 2-D PAGE maps. The majority of these proteins had molecular weights between 17.5 and 66 kDa, and most of them were distributed between the isoelectric points (pI) 4.0 and 5.9. Some protein spots were distributed in the more acidic region of the 2-D gel (pI <4.0). This pattern indicated that a number of proteins in the strain $D-8^T$ are acidic. To understand the adaptation mechanisms of moderately halophilic bacteria in response to sudden environmental changes, differential protein profiles of this strain were investigated by 2-D PAGE and $Imagemaster^{TM}$ 2D Platinum software after the cells were subjected to salt shock of 1 to 25% salinity for 5 and 50 min. Analysis showed 59 proteins with an altered level of expression as the result of the exposure to salt shock. Eighteen proteins had increased expression, S proteins were induced, and the expression of 33 proteins was down-regulated. Eight of the up-regulated proteins were identified using MALDI-TOF/MS and MASCOT, and were similar to proteins involved in signal transduction, proteins participating in energy metabolism pathways and proteins involved in stress.

• 한국압력기기공학회 논문집
• /
• 제13권2호
• /
• pp.75-83
• /
• 2017

In severe accident conditions of light water reactors, the loss of coolant may cause problems in integrity of zirconium fuel cladding. Under the condition of the loss of coolant, the zirconium fuel cladding can be exposed to high temperature steam and reacted with them by producing of hydrogen, which is caused by the failure in oxidation resistance of zirconium cladding materials during the loss of coolant accident scenarios. In order to avoid these problems, we develop a multi-metallic layered composite (MMLC) fuel cladding which compromises between the neutronic advantages of zirconium-based alloys and the accident-tolerance of non-zirconium-based metallic materials. Cold pilgering process is a common tube manufacturing process, which is complex material forming operation in highly non-steady state, where the materials undergo a long series of deformation resulting in both diameter and thickness reduction. During the cold pilgering process, MMLC claddings need to reduce the outside diameter and wall thickness. However, multi-layers of the tube are expected to occur different deformation processes because each layer has different mechanical properties. To improve the utilization of the pilgering process, 3-dimensional computational analyses have been made using a finite element modeling technique. We also analyze the dimensional change, strain and stress distribution at MMLC tube by considering the behavior of rolls such as stroke rate and feed rate.

• 한국광학회지
• /
• 제18권6호
• /
• pp.447-451
• /
• 2007

실리카 평판 광집적회로 마하젠더 간섭 구조를 이용한 트라이플렉서를 제안하고, 시뮬레이션을 통해 그 특성을 분석하였다. 1310 nm 대역과 $1480{\sim}1560nm$ 대역을 분리하기 위하여 마하젠더 암의 길이 차는 1310 nm 파장의 정수배 더하기 반 파장으로 하고, 방향성 결합기의 균형도는 $1480{\sim}1560nm$ 대역에서 적정화하였다. 이와 같은 마하젠더 간섭 구조를 한 단 더 사용함으로써, 매우 우수한 채널 누화 특성을 얻을 수 있었다. 1490 nm 대역과 1550 nm 대역을 추가적으로 분리하기 위하여 마하젠더 간섭구조를 추가로 두 단 더 사용하였다. 삼차원 BPM과 전송행렬방법을 통하여 각 채널들 사이의 낮은 누화 특성을 확인하였고, 제작 과정에서 발생할 수 있는 공정오차에 둔감한 특성을 보임을 확인하였다.