• Mycobiology
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• v.39 no.1
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• pp.33-39
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• 2011

Wild yeasts on the surface of various fruits including grapes were surveyed to obtain yeast strains suitable for fermenting a novel wine with higher alcohol content and supplemented with rice starch. We considered selected characteristics, such as tolerance to alcohol and osmotic pressure, capability of utilizing maltose, and starch hydrolysis. Among 637 putative yeast isolates, 115 strains exhibiting better growth in yeast-peptone-dextrose broth containing 30% dextrose, 7% alcohol, or 2% maltose were selected, as well as five ${\alpha}$-amylase producers. Nucleotide sequence analysis of the 26S rDNA gene classified the strains into 13 species belonging to five genera; Pichia anomala was the most prevalent (41.7%), followed by Wickerhamomyces anomalus (19.2%), P. guilliermondii (15%), Candida spp. (5.8%), Kodamaea ohmeri (2.5%), and Metschnikowia spp. (2.5%). All of the ${\alpha}$-amylase producers were Aureobasidium pullulans. Only one isolate (NK28) was identified as Saccharomyces cerevisiae. NK28 had all of the desired properties for the purpose of this study, except ${\alpha}$-amylase production, and fermented alcohol better than commercial wine yeasts.

• Journal of the Korean Society for Precision Engineering
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• v.22 no.1
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• pp.167-174
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• 2005

The design for cold extrusion dies is very important, because the die insert is subjected to very high radial and hoop stresses. The design of cold extrusion dies has many constrained conditions. In this paper, the used assumptions are such that the yield strength of each ring is selected according to the allowable tensile or compressive hoop stress in each ring and the maximum allowable inner pressure, when yielding occurs in one ring of the dies, is obtained by the proposed equation. In order to obtain design variables, such as diameter ratios and interferences, using the maximum inner pressure, the flexible tolerance method was used for shrink-fitted thick-walled cylinders. ANSYS APDL was used to perform the repeated analysis of deformation of the dies due to the variation of the design variables. The response surface methodology is utilized to analyze the relationship between the design variables and the maximum radial displacement of the die insert during extrusion. From the results, it is found that outer diameter of the die insert has the largest effect on the minimization of maximum radial displacement at the inner surface of the dies.

• Journal of Microbiology and Biotechnology
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• v.27 no.6
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• pp.1171-1179
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• 2017

Butanol is a promising alternative to ethanol and is desirable for use in transportation fuels and additives to gasoline and diesel fuels. Microbial production of butanol is challenging primarily because of its toxicity and low titer of production. Herein, we compared the transcriptome and phenome of wild-type Escherichia coli and its butanol-tolerant evolved strain to understand the global cellular physiology and metabolism responsible for butanol tolerance. When the ancestral butanol-sensitive E. coli was exposed to butanol, gene activities involved in respiratory mechanisms and oxidative stress were highly perturbed. Intriguingly, the evolved butanol-tolerant strain behaved similarly in both the absence and presence of butanol. Among the mutations occurring in the evolved strain, cis-regulatory mutations may be the cause of butanol tolerance. This study provides a foundation for the rational design of the metabolic and regulatory pathways for enhanced biofuel production.

• Plant Biotechnology Reports
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• v.4 no.4
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• pp.243-251
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• 2010

Nitric oxide (NO) has been shown to be involved in diverse physiological processes in microbes, animals and plants. In this study, the involvement of NO in the development and possible roles in oxidative stress protection of Chinese cabbage (Brassica rapa subsp. pekinensis cv. Samrack-ulgari) seedlings were investigated. Exogenous application of sodium nitroprusside (SNP) retarded root elongation, while increasing lateral root formation of Chinese cabbage. Plants showed no signs of external stress due to SNP application in true leaves. Cotyledons of 3-week-old Chinese cabbage plants were found to be highly sensitive to SNP application. Treated cotyledons displayed rapid tissue collapse and associated cell death. Although SNP application reduced root growth under normal growth conditions, it also enhanced methyl viologen (MV)-mediated oxidative stress tolerance. Analysis of SNP application to Chinese cabbage leaf disks, revealed SNP-induced tolerance against oxidative stresses by MV and $H_2O_2$, and evidence includes prevention of chlorophyll loss, superoxide anion (${O_2}^-$) accumulation and lipid peroxidation. This report supports a role for nitric oxide in modulating early seedling development, programmed cell death and stress tolerance in Chinese cabbage.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.16 no.1
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• pp.46-52
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• 2003

In this paper, we fabricated PRT(platinum resistance thermometers) with the trimming technology using high fine laser system. U. V.(wavelength： 355nm) laser was mainly used for adjusting Pt thin films resistors to 100Ω at 0$^{\circ}C$. Internationally, the accepted A-class tolerance of temperature sensor is ${\pm}$0.06Ω at 0$^{\circ}C$. according to DIN EN 60751. The width of trimmed lines was about 10$\mu\textrm{m}$ and the best trimming conditions of Pt thin films were power : 37mW, frequency : 200Hz and bite size：1.5$\mu\textrm{m}$. And 96 resistors, fabricated by photolithography and etching process, have 79∼90Ω and 91∼102Ω as the proportion of 45.7% and 57.3%, respectively. As result of sitting Pt thin films resistors to the target value(109.73Ω at 25$^{\circ}C$), 82.3% of all resistors had the tolerance within ${\pm}$0.03Ω and the others(17.7％) were within ${\pm}$0.06Ω of A-class tolerance. The PRTs which wore fabricated in this research had excellent characteristics as follows； high accuracy, international standard TCR(temperature coefficient of resistance) value, long-term stability, wide temperature range, good linearity and repeatability, rapid response time, etc.

• Transactions of the Society of Information Storage Systems
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• v.6 no.1
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• pp.12-17
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• 2010

Thermally-assisted magnetic recording (TAMR) is a potentially promising approach to increase the storage density of hard disk drive (HDD). However, TAMR has some issues such as temperature effects, media problems, thermal deformation and light delivery. In this research, we focused on light delivery. One of the powerful methods to deliver the light from laser diode to recording medium is the use of an optical fiber and a specially designed prism. However, the TAMR with optical fiber may have some flyability problems induced by the mounted optical fiber and prism. Also, the TAMR suspension using an optical fiber has high vertical and pitch stiffness. Therefore, p-torque is greatly increased by the optical fiber. Also, flying height (FH) of the slider with TAMR suspension can be largely changed by p-torque. Therefore, in this paper, we focus on the pitch static attitude (PSA) tolerance and the FH by PSA of the TAMR suspension. The FH is investigated using various errors and the PSA tolerance for TAMR suspension is proposed.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2000.11a
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• pp.245-248
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• 2000

Modern aircraft composite structures are designed using a damage tolerance philosophy. This design philosophy envisions sufficient strength and structural integrity of the aircraft to sustain major damage and to avoid catastrophic failure. The only reasonable way to treat on the same basis all the conditions and uncertainties participating in the design of damage tolerant composite aircraft structures is to use the probability-based approach. Therefore, the model has been developed to assess the probability of structural failure (POSF) and associated risk taking into account the random mechanical loads, random temperature-humidity conditions, conditions causing damages, as well as structural strength variations due to intrinsic strength scatter, manufacturing defects, operational damages, temperature-humidity conditions. The model enables engineers to establish the relationship between static/residual strength safety margins, production quality control requirements, in-service inspection resolution and criteria, and POSF. This make possible to estimate the cost associated with the mentioned factors and to use this cost as overall criterion. The methodology has been programmed into software.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.300-302
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• 2019

Glutamicibacter halophytocola DR408 isolated from the rhizospheric soil of soybean plant at Jecheon showed drought tolerance and plant growth promotion capacity. The complete genome of strain DR408 comprises 3,770,186 bp, 60.2% GC-content, which include 3,352 protein-coding genes, 64 tRNAs, 19 rRNA, and 3 ncRNA. The genome analysis revealed gene clusters encoding osmolyte synthesis and plant growth promotion enzymes, which are known to contribute to improve drought tolerance of the plant.

• Genomics & Informatics
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• v.19 no.4
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• pp.45.1-45.8
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• 2021

Brassica napus is the third most important oilseed crop in the world; however, in Korea, it is greatly affected by cold stress, limiting seed growth and production. Plants have developed specific stress responses that are generally divided into three categories: cold-stress signaling, transcriptional/post-transcriptional regulation, and stress-response mechanisms. Large numbers of functional and regulatory proteins are involved in these processes when triggered by cold stress. Here, our objective was to investigate the different genetic factors involved in the cold-stress responses of B. napus. Consequently, we treated the Korean B. napus cultivar Naehan at the 4-week stage in cold chambers under different conditions, and RNA and cDNA were obtained. An in silico analysis included 80 cold-responsive genes downloaded from the National Center for Biotechnology Information (NCBI) database. Expression levels were assessed by reverse transcription polymerase chain reaction, and 14 cold-triggered genes were identified under cold-stress conditions. The most significant genes encoded zinc-finger proteins (33.7%), followed by MYB transcription factors (7.5%). In the future, we will select genes appropriate for improving the cold tolerance of B. napus.

• Journal of KIISE:Information Networking
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• v.34 no.5
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• pp.305-315
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• 2007

Broadband Convergence Network (BcN) is the network which unifies telephone network, the Internet and broadcasting networks. Threats to each network can bring serious problems in BcN environment since the whole network can be damaged by various types of attack. The purpose of this study is to suggest the prototype of intrusion-tolerant network design of BcN to guarantee the continuous operation of BcN services against malicious attacks. First, BcN service components, selected by analysis of service time and coverage importance, are classified into three groups by their type: server type, gateway type and hybrid type. Second, the necessity of applying intrusion tolerance on BcN services is deduced by possible attack scenarios on BcN. Finally, we suggest the intrusion-tolerant network design suitable to BcN, using hardware redundancy and secure policies. Also, we present that the suggested network design can increase the intrusion tolerance of BcN.