• Title/Summary/Keyword: tolerance analysis

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Distributions of Endangered Fish Species and Their Relations to Chemical Water Quality-Ecological Stream Health in Geum-River Watershed (금강 대권역 대표 멸종위기 담수어류의 분포 특성 및 이화학적 수질-하천 생태건강도와의 관계분석)

  • Lee, Sang-Jae;An, Kwang Guk
    • Korean Journal of Environment and Ecology
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    • v.30 no.6
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    • pp.986-995
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    • 2016
  • The objective of this study was to analyze the distribution of endangered fish species and elucidate their relations on chemical water quality, physical habitat conditions and ecological stream health. The dominant species in the watershed was Pseudopungtungia nigra (Pn), Gobiobotia macrocephala (Gm), Gobiobotia brevibarba (Gb), Liobagrus obesus (Lo), and Iksookimia choii (Ic) in the order. The species of Pn designated as "critical endangered species (I) (CER)", was most widely distributed species among the endangered species, so the designation of the species should be re-evaluated. The endangered species was most popular (4 species, 384 individuals) in the Cho-River region of eighteen lotic regions. According to the analysis of chemical tolerance limits in the habitats with endangered fish species, biological oxygen demand (BOD) and total phosphorus (TP) was analyzed as "very good" (Ia) and "good condition" in the chemical criteria of the Ministry of Environment, Korea. Also, chemical conditions, based on ammonia-N ($NH_{4+}$), total nitrogen (TN), phosphate-P ($PO_{4^-}P$) were much better in the habitat with endangered species (Hw) than the habitat without endangered species (Ho). In the meantime, the species of Ic showed wide ranges on the chemical tolerance, so physical habitat conditions, such as the size of substrate particles (sand) and hydrological regime, were considered as more important factors than the chemical water quality, if the water quality is not largely degraded. The endangered species were also more distributed in the high-order (4-6) streams than the low-order (1-3) streams. The evaluation of ecological stream health, based on multi-metric model of the Index of Biological Integrity (IBI), showed the large difference between the Hw (21.6, fair condition)and Ho (30.5, good condition), indicating that the habitat maintained well chemically and physically had higher distributions of endangered species. Overall, the designation of CER on the Pn should be re-evaluated due to wide-distributions, and the protections from water pollution and the habitat conservations on the endangered species are necessary in the watershed.

Selection of (Ac/Ds) insertion mutant lines by abiotic stress and analysis of gene expression pattern of rice (Oryza sativar L.) (비생물학적 스트레스 관련 벼 Ac/Ds 삽입 변이체의 선발 및 유전자 발현 분석)

  • Jung, Yu-Jin;Park, Seul-Ah;Ahn, Byung-Ohg;Yun, Doh-Won;Ji, Hyeon-So;Lee, Gang-Sup;Park, Young-Whan;Suh, Seok-Cheol;Baek, Hyung-Jin;Lee, Myung-Chul
    • Journal of Plant Biotechnology
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    • v.35 no.4
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    • pp.307-316
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    • 2008
  • Transposon-mediated insertional mutagenesis is one of powerful strategy for assessing functions of genes in higher plants. In this report, we have selected highly susceptible and tolerance plant by screening about high salt (3% NaCl) and cold stresses ($4^{\circ}C$) from F2 seeds of 30,000 Ac/Ds insertional mutagenesis lines in rice (Oryza sativa L. cv. Dongjin). In order to identify the gene tagging, insertion of Ds element was analyzed by Southern blot and these results revealed that 19 lines were matched genotype of selected lines with phenotype from the first selected 212 lines, and 13 lines have one copy of Ds elements. The Franking Sequence Tags (FSTs) of selected mutant lines showed high similarities with the following known function genes: signal transduction and regulation of gene expression (transpoter, protease family protein and apical meristem family protein), osmotic stress response (heat shock protein, O-methyltransferase, glyceraldehyde-3-phosphate dehydrogenase and drought stress induce protein), vesicle trafficking (SYP 5 family protein) and senescence associated protein. The expression pattern of 19 genes were analyzed using RT-PCR under the abiotic stresses of 9 class; 250mM NaCl, osmotic, drought, 3% $H_2O_2$, $100{\mu}M$ ABA, $100{\mu}M$ IAA, 0.1 ppm 2,4-D, $4^{\circ}C$ cold and $38^{\circ}C$ high temperature. Isolated knock-out genes showed the positive response about 250 mM NaCl, drought, $H_2O_2$, PEG, IAA, 2,4-D, ABA treatment and low ($4^{\circ}C$) and high temperature ($38^{\circ}C$). The results from this study indicate that function of selected knock-out genes could be useful in improving of tolerance to abiotic stresses as an important transcriptional activators in rice.

Mitigation Effect of Drought Stress by Plant Growth-promoting Bacterium Bacillus sp. SB19 on Kale Seedlings in Greenhouse (식물생장촉진 Bacillus sp. SB19 균주의 케일 처리에 대한 가뭄 스트레스 완화 효과)

  • Kim, Dayeon;Lee, Sang-Yeob;Kim, Jung-Jun;Han, Ji-Hee
    • Korean Journal of Organic Agriculture
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    • v.24 no.4
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    • pp.833-847
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    • 2016
  • Drought stress is a major agricultural limitation to crop productivity worldwide, especially by which leafy vegetables, plant leaves eaten as vegetable, could be more lethal. The study was carried out to know the effect of drought tolerance plant growth promoting bacteria (PGPB) on water stress of kale seedlings. A total of 146 morphologically distinct bacterial colonies were isolated from bulk soil and rhizosphere soil of leafy vegetables and screened for plant growth promoting microbioassay in greenhouse. Out of them the isolate SB19 significantly promoted the growth of kale seedlings in increasement of about 42% of plant height (14.1 cm), 148% of leaf area ($19.0cm^2$) and 138% of shoot fresh weight (1662.5 mg) attained by the bacterially treated plants compared to distilled water treated control (9.9 cm, $7.7cm^2$, 698.8 mg). Shoot water content of SB19 treated kale seedlings (1393.8 mg) was also increased about 152% compared with control (552.5 mg). The SB19 isolated from bulk soil of kale plant in Iksan, Korea, was identified as species of Bacillus based on 16S rRNA gene sequencing analysis. We evaluated the effect of drought tolerance by the Bacillus sp. SB19 on kale seedlings at 7th and 14th days following the onset of the water stress and watering was only at 7th day in the middle of test. In the survey of 7th and 14th day, there were mitigation effect of drought stress in kale seedlings treated with $10^6$ and $10^7cell\;mL^{-1}$ of SB19 compared to distilled water treated control. Especially, there were more effective mitigation of drought damage in kale seedlings treated with $10^7cell\;mL^{-1}$ than $10^6cell\;mL^{-1}$. Further, although drought injury of bacterially treated kale seedlings were not improved at 14th day compared with 7th day, drought injury of $10^7cell\;mL^{-1}$ of SB19 treated kale seedlings were not happen rapidly but developed over a longer period of time than $10^6cell\;mL^{-1}$ of SB19 or control. The diffidence of results might be caused by the concentration of bacterial suspension. This study suggests that beneficial plant-microbe interaction could be a important role of enhancement of water availability and also provide a good method for improving quality of leafy vegetables under water stress conditions.

Soil Loss and Pollutant Load Estimation in Sacheon River Watershed using a Geographic Information System (GIS를 이용한 동해안 하천유역의 토양유실량과 오염부하량 평가 -사천천을 중심으로-)

  • Cho, Jae-Heon;Yeon, Je-Chul
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.7
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    • pp.1331-1343
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    • 2000
  • Through the integration of USLE and GIS, the methodology to estimate the soil loss was developed, and applicated to the Sacheon river in Gangrung. Using GIS, spatial analysis such as watershed boundary determination, flow routing. slope steepness calculation was done. Spatial information from the GIS application was given for each grid. With soil and land use map, information about soil classification and land use was given for each grid too. Based upon these data, thematic maps about the factors of USLE were made. We estimated the soil loss by overlaying the thematic maps. In this manner, we can assess the degree of soil loss for each grid using GIS. Annual average soil loss of Sacheon river watershed is 1.36 ton/ha/yr. Soil loss in forest, dry field, and paddy field is 0.15 ton/ha/yr, 27.04 ton/ha/yr, 0.78 ton/ha/yr respectively. The area of dry field, which is 4% of total area, is $2.4km^2$. But total soil loss of dry field is 6561 ton/yr, and it occupies 84.9 % of total soil loss eroded in Sacheon river watershed. Comparing with the 11.2 ton/ha/yr of an average soil loss tolerance for cropland, provision for the soil loss in dry field is necessary. Run-off and water quality of Sacheon river were measured two times in flood season: from July 24, 1998 to July 28 and from September 29 to October 1. As the run-off of the river increased, SS, TN, TP concentrations and pollutant loadings increased. SS, TN, TP loads of Sacheon river discharged during the 2 heavy rains were 21%, 39%, and 19% of the total pollutant loadings generated in the Sacheon river watershed for one year. We can see that much pollutants are discharged in short period of flood season.

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Effects of Maltogenic Amylase on Textural Properties of Dough and Quality Characteristics of White Pan Bread (Maltogenic Amylase가 식빵반죽의 물성과 식빵의 품질 특성에 미치는 영향)

  • Yoon, Seongjun;Cho, Namji;Lee, Soo-Jeong;Moon, Sung-Won;Jeong, Yoonhwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.5
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    • pp.752-760
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    • 2015
  • Effects of maltogenic amylase on textural properties of dough and quality characteristics of white pan bread were investigated. White pan bread was prepared with four different levels of maltogenic amylase contents (M-1: 0.048 U/g, M-2: 0.060 U/g, M-3: 0.072 U/g, M-4: 0.084 U/g). The setback by amylograph for the control was $480.0{\pm}12.25$ Brabender Unit (B.U.) while M-4 showed the a setback of $215.0{\pm}5.00B.U.$ The absorption, mixing tolerance index, and stability by farinogram were not significantly different (P>0.05) for across all treatments. The area under the curve (135 min) by extensogram was higher than all samples. The texture profile analysis results showed that there was significant decreasing in hardness for the maltogenic amylase infused bread (P<0.05). M-3 and M-4 showed higher springiness and cohesiveness but lower hardness than control over 1 to 3 days, indicating possibly extended shelf-life. Imaging scan showed that air cell size less than $0.4mm^2$ for the control and M-4 were at rates of 94.90% and 95.70%, respectively. For sensory evaluation, M-3 and M-4 showed higher intensities than the control for taste, flavor, texture, mouthfeel, and moistness quality. These results imply that the quality of white pan bread could be improved by adding maltogenic amylase without the use of chemical additives.

Clinical significance of acanthosis nigricans in children and adolescents with obesity induced metabolic complications (비만으로 인한 대사적 합병증을 가진 소아 및 청소년에서 흑색가시세포증의 임상적 의의)

  • Chueh, Hee Won;Cho, Gyu Rang;Yoo, Jaeho
    • Clinical and Experimental Pediatrics
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    • v.50 no.10
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    • pp.987-994
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    • 2007
  • Purpose : This study investigated the clinical significance of AN in children and adolescents with obesity induced metabolic complications. Methods : Forty-nine patients who had obesity induced metabolic complications were participated in this cross-sectional study. Obesity induced metabolic complications are as follows: hypertension, dyslipidemia, impaired fasting glucose (IFG), impaired glucose tolerance (IGT), nonalcoholic steatohepatitis (NASH), homeostasis model assessment of insulin resistance (HOMA-IR)>3.16. Clinical characteristics, such as, age, percentage-weight-for-height (PWH), pubertal status, blood pressure (BP), fasting plasma insulin level, fasting and post-oral glucose tolerance test 2-hour glucose levels, liver function test, lipid profile, HOMA-IR were compared according to the presence of AN. Results : Sixty-five percent of patients had AN, 57.1% NASH, 57.1% dyslipidemia, 55.1% hypertension, 46.9% IFG, 24.5% HOMA-IR>3.16 and 16.2% IGT. The patients who were moderately to severely obese with AN had higher incidence of IGT and HOMA-IR>3.16. The patients with AN had significantly higher diastolic BP ($79.4{\pm}6.9$ vs $75.4{\pm}5.6mmHg$), fasting levels of plasma insulin ($10.6{\pm}6.0$ vs $6.2{\pm}5.4{\mu}IU/mL$), HOMA-IR index ($2.6{\pm}1.4$ vs $1.4{\pm}1.3$) and PWH ($42.4{\pm}13.0$ vs $34.3{\pm}1.8%$). The increasing tendency for the presence of AN was significantly related to the cumulative number of obesity induced metabolic complications. Binary logistic regression analysis revealed that the presence of AN was significantly associated with fasting plasma insulin level, PWH and IFG. Conclusion : AN could be useful as a clinical surrogate of obesity induced metabolic complications.

Effect of Salinity Stress on Growth, Yield, and Proline Accumulation of Cultivated Potatoes (Solanum tuberosum L.) (염 스트레스에 따른 감자 품종 (Solanum tuberosum L.) 간 생육, 수량 및 proline 함량 변이)

  • Im, Ju Sung;Cho, Ji Hong;Cho, Kwang Soo;Chang, Dong Chil;Jin, Yong Ik;Yu, Hong Seob;Kim, Wha Yeong
    • Horticultural Science & Technology
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    • v.34 no.6
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    • pp.818-829
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    • 2016
  • This study evaluated the responses of 18 potato cultivars to three levels of salinity stress (electrical conductivity, EC: 1.0, 4.0, and $8.0dS{\cdot}m^{-1}$). Stem, leaf, root, chlorophyll, tuber yield, and proline content were investigated and statistically analyzed using analysis of variance (ANOVA) and correlations. Stem number and stem diameter were not affected by salinity, but stem length and aerial weight showed highly significant responses to salinity. Aerial weight decreased with increasing salinity levels in most cultivars, while it increased in some the cultivars 'Daejima', 'Goun', 'Haryeong', and 'LT-8'. Leaf number, leaf area index, and leaf weight were most significantly affected by salinity and the cultivar ${\times}$ salinity interaction. Root length, root weight, total chlorophyll and chlorophyll a were affected by salinity, but not by the cultivar ${\times}$ salinity interaction. The opposite trend was shown in chlorophyll b. Although there was great variability among cultivars, tuber yield decreased in all cultivars, and was most significantly influenced by salinity and the cultivar ${\times}$ salinity interaction. 'Superior', 'Kroda', 'Romana', and 'Duback' gave better tuber yields under salinity at EC 4.0 and $8.0dS{\cdot}m^{-1}$ than the cultivars with better aerial weights. Proline content was increased by salinity in all cultivars, and was more remarkable in the cultivars with better aerial weights than in cultivars such as 'Superior' and 'Kroda' with better tuber yields. Leaf number, leaf area index, leaf weight, and root length parameters were considered to be useful criteria in the evaluation of salt tolerance because of their high positive correlation with tuber yield; however, given its negative correlation with tuber yield under high salinity, proline content was not. Salinity tolerances varied greatly among potato cultivars. The low correlation between growth and yields of aerial parts under high salinity suggests that, in commercial agriculture, it might be more practical to compare relative yields to controls. Additionally, 'Superior', 'Kroda', 'Romana', and 'Duback' might be very useful cultivars to use in breeding programs to develop salinity-tolerant potatoes, as well as for sustainable potato production in saline areas.

The Development of Quality Assurance Program for CyberKnife (사이버나이프의 품질관리 절차서 개발)

  • Jang, Ji-Sun;Kang, Young-Nam;Shin, Dong-Oh;Kim, Moon-Chan;Yoon, Sei-Chul;Choi, Ihl-Bohng;Kim, Mi-Sook;Cho, Chul-Koo;Yoo, Seong-Yul;Kwon, Soo-Il;Lee, Dong-Han
    • Radiation Oncology Journal
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    • v.24 no.3
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    • pp.185-191
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    • 2006
  • [ $\underline{Purpose}$ ]: Standardization quality assurance (QA) program of CyberKnife for suitable circumstances in Korea has not been established. In this research, we investigated the development of QA program for CyberKnife and evaluation of the feasibility under applications. $\underline{Materials\;and\;Methods}$: Considering the feature of constitution for systems and the therapeutic methodology of CyberKnife, the list of quality control (QC) was established and divided dependent on the each period of operations. And then all these developed QC lists were categorized into three groups such as basic QC, delivery specific QC, and patient specific QC based on the each purpose of QA. In order to verify the validity of the established QA program, this QC lists was applied to two CyberKnife centers. The acceptable tolerance was based on the undertaking inspection list from the CyberKnife manufacturer and the QC results during last three years of two CyberKnife centers in Korea. The acquired measurement results were evaluated for the analysis of the current QA status and the verification of the propriety for the developed QA program. $\underline{Results}$: The current QA status of two CyberKnife centers was evaluated from the accuracy of all measurements in relation with application of the established QA program. Each measurement result was verified having a good agreement within the acceptable tolerance limit of the developed QA program. $\underline{Conclusion}$: It is considered that the developed QA program in this research could be established the standardization of QC methods for CyberKnife and confirmed the accuracy and stability for the image-guided stereotactic radiotherapy.

Changes in Sleep Patterns and Mood States of Shift Workers Following Nocturnal Light Exposure (교대근무자에서 야간 광 노출에 따른 수면양상 및 기분상태 변화)

  • Kwon, Ki-Bum;Yoon, In-Young;Kang, Sang-Bum;Jeong, Do-Un
    • Sleep Medicine and Psychophysiology
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    • v.6 no.1
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    • pp.68-75
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    • 1999
  • Objectives: We intended to observe changes in sleep patterns and mood states of night-shift workers following light exposure. We also estimated the degree of tolerance of light exposure. By studying these, we investigated the possibility of applying light therapy to night-shift workers for improving their adaptation. Methods: Twelve night-shift nurses working at Yong-In Mental Hospital volunteered to participate in this study. The study consisted of 3 parts: 1) night-shift control study; 2) light exposure study; 3) day-shift control study. All the nurses accomplished 3 parts of the study, each of which continued for 3 days, except one nurse who did not participate in day-shift control study. During light exposure study, nurses were exposed to bright light for 4 hours from 1AM to 5AM. Sleep patterns were evaluated with wrist actigraphy and automatic sleep analysis program. Mood states and side effects of light exposure were assessed with self-report scales. Results: Sleep period time, total sleep time, and sleep efficiency were increased following light exposure compared with night-shift control study. Light exposure study showed no difference from day-shift control study in above-mentioned sleep parameters. Daily fluctuation of sleep efficiency was less prominent during light exposure study than during night-shift control study. During light exposure study, the subjects felt more elated and energetic in the evening after daytime sleep than during night-shift control study. None of the subjects complained of severe side effects related to light exposure on the third day of light exposure. Tolerance of side effects was noted to develop with the repetition of light exposure. Conclusion: Light exposure improved the daytime sleep of night-shift workers to the level of normal nighttime sleep, making the subjects more elated and energetic. Side effects of light exposure were found to be tolerable. Light exposure seems to be safely applicable to night-shift workers for their adaptation.

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The Effect of the Subjective Wellbeing on the Addiction and Usage Motivation of Social Networking Services: Moderating Effect of Social Tie (SNS 이용동기와 SNS 중독이 주관적 웰빙에 미치는 영향: 사회적 유대감의 조절효과)

  • Noh, Mi-Jin;Jang, Sung-Hee
    • Management & Information Systems Review
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    • v.35 no.4
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    • pp.99-122
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    • 2016
  • The social networking services (SNSs) have become popular among smartphone users, and one of the most popular services. In order to explain users' motivations toward SNS, this study considers uses and gratification theory which can explain individuals' motivations to select certain media channels. The purposes of this study is to investigate the relationships between motivations and addiction of SNS, and between addiction of SNS and decline in the subjective wellbeing. We examine moderating effects of social tie based on the social capital theory in the relationships between SNS addiction and decline in the subjective wellbeing. The motivations of SNS are subdivided into emotional motive (entertainment and fantasy) and cognitive motive (information share burden and challenge burden) based on the use and gratifications theory. The addiction of SNS is subdivided into time tolerance, withdrawal symptoms, interruption, and barrier of living. The data used in this study were collected from 286 SNS users through surveys. The data analysis in this study was performed using AMOS 17.0, and we used SEM(Structural Equation Modeling) methods in order to test the research model. The result shows that the emotional motive(entertainment and fantasy) and cognitive motive(information share burden and challenge burden) have an effect on the addiction of SNS. Especially emotional motive such as entertainment and users' fantasy toward SNS is an important factor that can cause SNS addiction. The addiction of SNS such as time tolerance, withdrawal symptoms, interruption, and barrier of living has an effect on the decline in the subjective wellbeing. Our result show that social tie partially moderates the relationship SNS addiction and decline in the subjective wellbeing. In addition, social tie between interruption of SNS and decline in the subjective wellbeing is an important moderating factor. The results focuses on the understanding toward relationship between SNS addiction based on the online and decline in the subjective wellbeing in the real world. The findings of this study also provides theoretical as well as practical implications which reflect the major features of SNS, and moderating effects of social tie based on the social capital.

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