• 한국연초학회지
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• 제9권2호
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• pp.25-35
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• 1987

Effects of application time And rate of chlorine on the chlorine absorption and distribution at different stalk positions of the plants were Investigated in burley tobacco grown under the pot condition. Treatment consisted of 6 application times (from 2 weeks before transplanting to 8 weeks after transplanting with 2 weeks' interval) and 2 application rates (2g and 4g-KCl/pot) by 6X2 factorial experimental design. The tobacco plants took up the chlorine rapidly just after chlorine application, and the chlorine content of leaf was increased proportionally to the rate of chlorine application. The chlorine was accumulated when much in lower leaves when chlorine was applied early, while the chlorine accumulated much in upper leaves when chlorine was applied late. The chlorine content of leaf was negatively correlated to the degree of brightness, red color and yellow color of cured leaf. Significant Increases In cured leaf ratio, leaf thickness, leaf weight and weight Per unit leaf area of harvested leaf were obtained with increased chlorine application. When the chlorine was applied early, the leaf weight showed Increasing tendency.

• Mycobiology
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• 제29권1호
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• pp.48-53
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• 2001

Plants have the ability to acquire an enhanced level of resistance to pathogen attack after being exposed to specific biotic stimuli. To obtain plant growth-promoting rhizobacteria inducing resistance against cucumber anthracnose by Colletotrichum orbiculare, more than 800 strains of rhizobacteria were screened in the greenhouse. Among these strains, Bacillus amyloliquefaciens solate EXTN-1 showed significant disease control efficacy on the plants. Induction of pathogenesis-related(PR-la) gene expression by EXTN-1 was assessed using tobacco plants transformed with PR-1a::$\beta$-glucuronidase(GUS) construct. GUS activities of tobacco treated with EXTN-1 and salicylic acid-treated transgenic tobacco were significantly higher than those of tobacco plants with other treatments. Gene expression analyses indicated that EXTN-1 induces the accumulation of defense-related genes of tobacco. The results showed that some defense genes are expressed by the treatment with EXTN-1 suggesting the similar resistance mechanism by salicylic acid.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
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• pp.289.1-289
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• 1997

No Abstract, See Full Text

• 환경영향평가
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• 제22권2호
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• pp.125-134
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• 2013

Plant virus coat (CP) gene-mediated protection is one of the best known approaches to protect against virus resistant transgenic plants. Transgenic N. benthamiana plants containing the CP gene of Zucchini green mottle mosaic virus (ZGMMV) were used for the environmental risk assessment of the living modified (LM) plants with plant virus resistance. The most optimal co-infection method of both ZGMMV and CMV (Cucumber mosaic virus) on Non-LM and CP-expressing LM tobacco plants was established and co-infection of CMV and ZGMMV was confirmed by polymerase chain reaction (PCR). To address the effects of LM tobacco plants on the mutation of the virus, in-vitro transcripts of CP and Replicase (Rep) derived from CMV and/or ZGMMV were inoculated onto Non-LM or LM tobacco plants. Mutation frequency of CP and Rep from CMV and ZGMMV was examined through six serial passages in Non-LM and LM tobacco plants. Little actual frequency of mutation was estimated, probably due to the limited number of transgenic plants tested in this study. However, it does not suggest environmental safety of these CP-mediated LM plants. Further study at a larger scale is needed to evaluate the environmental risk associated with the CP-expressing LM plants.

• The Plant Pathology Journal
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• 제15권1호
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• pp.8-13
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• 1999

Hypersensitive cell death of plants during incompatible plant-pathogen interactions is one of the efficient defense mechanisms of plants against pathogen infections. For better understanding of the molecular mechanisms involved in the plant hypersensitive response (HR), TMV-induced biotic plant cell death and CuSO4-induced abiotic plant cell death were compared in terms of expression patterns of ten different defense-related genes as molecular markers. The genes include five pathogenesis-related protein genes, two plant secondary metabolite-associated genes, two oxidative stress-related genes and one wound-inducible gene isolated from tobacco. Northern blot analyses revealed that a same set of defense-related genes was induced during both biotic and abiotic cell death but with different time and magnitude. The expression of defense-related genes in tobacco plants was temporarily coincided with the time of cell death. However, when suspension cell cultures was used to monitor the expression of defense-related genes, different patterns of the gene expression were detected. This result implies that three are common and, in addition, also different branches of signaling pathways leading to the induced expression of defense-related genes in tobacco during the pathogen- and heavy metal-induced cell death.

• 한국연초학회지
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• 제13권1호
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• pp.53-60
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• 1991

Seedling rot occurred on burley tobacco at the early growing stage after transplanting was surveyed in the Chonbuk province, the main area for burley tobacco production, during the 3 crop years from 1986. The incidence of disease was about 0.6-0.7% every year and major causal organisms were Pythium spp. More diseased plants were observed in the fields in which tobacco planted with improved mulching system than in that with any other mulching system. The temperature and relative humidity in the planting hole covered with polyethylene film for 11 days after transplanting were recorded by 27-45$^{\circ}C$ and 75~95%, respectively. The punched polyethylene film mulching field showed less diseased plants than that with improved mucking. In improved mulching system the longer period of mulching caused higher incidence of seedling rot(r=0.74**). Soil drench treatment of 200m1 of aqueous Metalaxyl Solution(125ppm) was effective to control the disease.

• 한국식물병리학회지
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• 제10권4호
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• pp.292-296
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• 1994

Main tobacco diseases were surveyed on tobacco plants in the northern Kyeongbuk area to evaluate the effectiveness of current disease control techniques from 1993 to 1994. The major disease in seedlings was the anthracnose (Colletotrichum tabacum) but the mosaic (TMV) in adult plants. Wild fire (Psedomonas syringae pv. tabaci) and hollow stalk (Erwinia carotovora subsp. carotovora) were more severe in 1993 than those in 1994 because of more rain. Establishment of drain-outlet at the bottom of the temporary transplanting pot gave a significant reduction in anthracnose severity. Most farmers have ignored the protection effect of using milk during transplanting and the removal of diseased roots from the fields on mosaic (TMV). It was considered that the educating program on disease diagnosis and fungicide application might help farmers for the proper use of chemicals.

• 생약학회지
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• 제28권4호
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• pp.192-197
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• 1997

As a part of searching for new antitumor agents from natural products, 94 kinds of Chinese plants were extracted with petroleum ether/ether (1:1), ethyl acetate and methanol, successively and their cytotoxicities were evaluated against A549 (human lung carcinoma) cell line. Among them, six kinds of ether extracts, seven kinds of ethyl acetate extracts and one kind of methanol extracts showed significant cytotoxic activities (above 70% inhibition) at a concentration of $50\;{\mu}g/ml$. These results surest that they may be involved in natural sources with possible anticancer activities.

• 한국연초학회:학술대회논문집
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• 한국연초학회 1997년도 담배과학 국제학술대회
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• pp.134-152
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• 1997

담배 모자이크 바이러스(TMV)와 감자 바이러스 Y(PVY) 등 식물바이러스병은 잎담배 생산에 심한 경제적 손실을 초래하고 있다. 바이러스병 방제를 위한 여러 가지 경종적 방법은 충분한-방제 효과를 얻지 못하는 경우가 많아 바이러스의 방제에는 우수한 저항성 품종의 사용이 가장 바람직한 것으로 알려져 있다. 그러나 기존의 육종 방법이 저항성 품종 개발에 항상 바람직한 것은 아닌데 그 이유는 저항성 유전자원이 없는 경우가 많고 또한 육종을 통해 유전자원의 열등한 특성이 도입될 수 있기 때문이다. 따라서 본 연구실에서는 TMV와 PVY의 여러 가지 형태의 외피단백질(CP) (비전사부분 포함 또는 제거, 비 전사부분 등) 및 복제유전자(Nlb) (3' 및 5' 결손 유무, 돌연변이)를 상용 담배 품종인 NC 82와 Burley 21에 형질전환시켜 바이러스 저항성 개발을 시도하였다. 각각의 유전자 cDNA를 1-2개의 35S promotor를 가진 식물발현벡타에 클로닝한 후 Agrobacteriupn (upnefaciens LBA 4404를 이용하여 식물의 잎조직에 도입시킨 후 kanamycin 함유 MS 배지에서 식물체를 재닥화하였다. 재분화 식물체는 TMV와 PVY에 대한 저항성 검정을 하였다. 그 결과 TMV에 저항성인 TMV CP 형질전환 식물체와 8 가지 Nlb 형질전환 식물체 계통 중에 6 계통의 저항성 형질전환 식물체를 획득하였다. 여기에서는 TMV와 PVY의 접종시험을 통하여 각각의 바이러스에 대한 형질전환 담배의 계통별 저항성 정도를 조사하고, 저항성 형질전환 식물체에서의 도입 유전자 확인하며, 세대별 저항성의 유전 및 안정성을 살펴보고자 한다. 또한 유망 저항성 형질전환 계통의 식물체 내에서의 바이러스 증식 및 이동과 관련된 저항성 기작, 여러 가지 PVY 계통에 대한 저항성 유무, 수량, 생육 특성 및 주요 화학 성분 함량 등을 발표하고자 한다.-glucose로 구성된 다당류 이었다. 아미노산은 Asp 및 Glu의 산성 아미노산과 Ala, Leu 등의 함량이 높게 나타났으며, 비알칼리 추출물에서 Ser과 Thr의 함량이 높게 나타났다. 다당류 T-AS는 평균 분자량이 2,000 kD와 12kD에서 주 peak를 나타냈으며, 수용성 분획의 평균 분자량은 12kD이고 비수용성 분획은 36~2,000 kD의 평균 분자량 분포를 갖는 것으로 나타났다. IR과 NMR 분석 결과 890 cm-1에서 흡수 peak를 나타내어 $\beta$-(1,3)0glucan과 $\beta$-(1,6)-glucan의 구조를 갖는 다당류로 확인 되었다. T-AS 분획은 C:H:O:N의 함량비가 38.9:5.7:49.6:1.84%이며, 이 물질의 융점은 163 $^{\circ}C$로 연한 갈색을 나타낸다. 분리된 GLG의 항암활성 기전 규명을 위해, in vivo 항암실험, 항보체 활성능, 항체 생성능, serum protein 분비능, 대식세포의 탐식능과 활성능 및 세포간 물질 분비 등의 상관관계를 조사하였다. 다당류 GLG 분획물들 가운데 항보체의 활성이 높았던 분획은 sarcome 180에 대한 항암 활성이 높게 나타났다. 다당류 T-AS의 보체 활성화 기작은 classical과 alternative complement pathway의 양 경로를 통해 활성화 되었다. T-AS 분획은 mouse내의 특정 혈청단백을 증가시켰으며, 항체 생성능의 증가가 관찰되어 effect T 세포의 활성화가 나타나고 있음을 알 수 있었다. T-AS는 생체내 투여시에 대식세포의 탐식능이 증진되었으며, 대식세포 기능 저해제에 의한 대식세포의 기능 저해 현상이 회복되었다. 이와 같은 결과들로부터, T-AS의 항암 활성은 활성화된 보체 성분 및 당 수용체들이 존재하는 대식세포의 개입을 시사한다.가능성과

• 한국응용곤충학회지
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• 제14권2호
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• pp.59-63
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• 1975

우리나라 잎담배에 발생하고 있는 바이러스의 종류와 감염상을 조사하기 위하여 전주지방에서 Burley종 (Burley-21) 20개체, 부천연초시험장 포장에시 황색종(Hicks) 20개체를 병징별로 채집하여 혈청학적인 방법에 의해 실시했다. 검정대상 바이러스는 Tobacco mosaic virus(TMV), Cucumber mosaic virus(CMV), Alfalfa mosaic virus (AMV), Potato virus X(PVX) 및 Potato virus Y(PVY)등 5종으로 TMV, AMV, CMV는 한천내확산법으로 PVX, PVY는 미량침강법으로 실시하여 다음과 같은 결과를 얻었다. 1. 공시이병개체중에서 TMV, CMV, AMV, P X 및 PVY 등 5종의 바이러스가 검출되었다. 2. 조사된 40개체중 각 바이러스의 감염율은 AMV $67.5\%$, CMV $60.0\%$, TMV$47.5\%$PVY$17.5\%$, PVX$10.0\%$의 순이었다. 3. Burley종에서는 TMV감염율이 $15.0\%$로 황색종의 $80.0\%$보다 현저히 낮았다. 4. 조사된 각 개체는 단독감염으로부터 2-4종의 바이러스로 다양하게 혼합감염을 나타냈으며 이 중 단독감염이 $37.5\%$, 혼합감염이 $62.5\%$이었다.