• Journal of Plant Biology
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• 제30권4호
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• pp.287-298
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• 1987

The regenerative capacities of protoplasts isolated from potato (Solamum tuberosum L.) tubers and tobacco (Nicotiana tabacum L.) mesophyll tissues were examined, and then their intergeneric protoplast fusion was carried out. The potato tuber-derived protoplasts proliferated into the calli some of which showed rudimentary shoot-like structures, which had not been attempted before from tubers, while the tobacco protoplasts were regenerated into the whole plants. Intergeneric protoplast fusion between potato and tobacco was carried out and the heteroplasmic fusion products were formed. The first cell division of some of them was observed after 5 days of culture.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1990년도 추계 학술대회지
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• pp.42-43
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• 1990

• 한국연초학회지
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• 제17권2호
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• pp.109-113
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• 1995

Nicotiana repanda has resistance to many important tobacco diseases but no tobacco cultivars are currently available that carry risistance genes derived from this species. Numerous attempts to hybridize N, repanda with commercial tobacco cultivars have been largely unsuccessful because of cross incompatibility or the uncovering of lethal genes. In vitro pollination of placenta attached ovules was useful in by passing prezygotic barriers for interspecific hybrid combination between N.tabacum cv. NC82 and N. repanda. Six days after in vitro pollination of N. tabacum cv. NC82×N. repanda, enlarged ovules on plancenta were removed and transferred into ovule culture medium of kitsch and kitsch (1969). Within 15 days of ovule culture, germination occurred. Most of the hybrid seedlings obtained had poor root system and finally died, while few of them had good root system and grew well.

• 한국연초학회지
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• 제1권1호
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• pp.9-13
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• 1979

담배品種 ( Nicotiana tabacum L.) "SC 72"와 "Hicks" 를 文配하여 그$F_1$ 잡종식물의 葯으로부터 誘起한 반수체배가계통들의 특성을 檢定하였던 바, 交配親의 平均値에 비하여 生育特性은 현저히 저하하고, Total Alkaloids 는 증가하며, Reducing Sugar는 감소하는 傾向을 나타내었다.

• 한국연초학회지
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• 제15권2호
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• pp.123-129
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• 1993

Mesophyll protoplasts derived from young leaves of Nicotiana rustica and N. tabacum cv Burley 21 were fused with the aid of polyethylene glycol(PEG). Cytological examination of protoplasts after PEG treatment revealed 12.8 % heterokaryocytes. After 7 weeks culture, the hybrid calli showing greenish white with a compact appearance were selected in contrast to parental type calli tinged with white or green color. The somatic hybrid plants were verified by morphological, biochemical and cyclological analysis. A heterosis effect for plant vigor and height was observed but the shape of leaves and flower characteristics were intermediate between N. tabacum and N. rutstica. The isozyme banding patterns for peroxidase of somatic hybrid lines were compared with the parent species. A number of isozyme bands derived from both parental species were found in the hybrids. Somatic hybrid plants have been successfully backcrossed to the parental N. tabacum particularly with somatic hybrid plants as female parents. These hybrid plants yielded small seeds, only few which were germinable.

• 한국작물학회지
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• 제11권
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• pp.11-80
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• 1972

• 한국작물학회:학술대회논문집
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• 한국작물학회 1988년도 춘계 학술대회지
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• pp.94-95
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• 1988

• 한국연초학회지
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• 제22권1호
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• pp.39-44
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• 2000

This study was carried out to obtain the basic information about the sucker producing characteristics of certain burley tobacco varieties(Nicotiana tabacum L. var. burley) at Suwon and Chonju Experiment Station, Korea Ginseng & Tobacco Research Institute in 1999. The number and weight of suckers for 12 varieties or breeding lines were investigated three times, 45 days after transplanting for ground sucker, 10 days after topping, and 10 days after maleic hydrazide(MH) applying for upstalk sucker. KB 103 and KB 9416-1 produced less ground suckers and upstalk suckers than other entries did. KB 9210-8 produced less ground suckers and (Male sterile TC 612 x KB 108)F$_1$ also produced less upstalk suckers than other entries did. There was no differences among entries in sucker production at 10 days after MH treatment. Between the weight of total sucker and days to flower revealed the negative correlation, but not significant. The low sucker producing entries described above could be used as a parental line in the breeding program for the low sucker producing variety.

• 한국연초학회지
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• 제16권2호
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• pp.102-107
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• 1994

This study was conducted to transfer the potato virus Y-necrosis strain resistance from Nicotiana africana to Nicotiana tabacum (cv. NC82) N. africana was crossed with NC82, GErmination of the cotyledon stage, at which time most of the seedling died. However, surviving seedlings continued to grow normally. Chromosomes of the these interspecific self-sterile F1 hybrids were doubled by tissue culture. Amphidiploid of F1 hybrid was self-fertile. Starting with amphidipliod, a wystematic backcross (BC) program was set up with NC82 as recurrent parent. In the BC5C2 generation, the resistant plant was selected. This resistant line, KF8833-1, had 48 chromosome and secreting glandular trichomes. It flowered 2days later than NC82, and stalk height, leaves per plant, leaf length and leaf width were similar to those of NC82.

• 한국연초학회지
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• 제1권1호
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• pp.46-50
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• 1979

4種의 Burley種 細胞質的 雄性不稔系統과 稔性品種의 특성을 圃場에서 검정하였던 바, 雄性不稔系統들의 農耕的 化學的 특성이 稔性品種과 近似하여 , Fl 雜種 이용을 위한 交配母本으로 사용할 수 있을 것으로 考察된다.