• 한국동물위생학회지
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• 제23권2호
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• pp.113-124
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• 2000

A total of 500 sera and lungs of slaughtered pigs were examined to investigate antibody titer, prevalence of pulmonary lesion, and interrelation among lung lesion score, type of pulmonary lesion and antibody titers by ELISA. The results obtained were as follows ; 1. The highest distribution of antibody titer was showed at 20 - 80 in M hyopneumoniae, 160-640 in P multocida type A and 160 - 640 in A pleuropneumoniae serotype 2 and 5. 2. The prevalence of pulmonary lesions was 84.0%, mean pulmonary lesion and mean lung score listed as 24.0$\pm$19.8% and 2.5$\pm$1.6, respectively. 3. In the prevalence of type of pulmonary lesion, enzootic pneumonia, pleuropneumonia and pleuritis were 58.2%, 10.0% and 15.8%, respectively. 4. Lung lesion score and type of pulmonary lesion were not interrelated with the distribution of antibody titer to specific pathogens, and causative pathogens of respiratory diseases were complicated with various bacteria.

• Childhood Kidney Diseases
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• 제26권2호
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• pp.86-90
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• 2022

Antineutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis requires prompt diagnosis and treatment, since renal function at the time of diagnosis is significantly associated with renal outcomes. Here, we report two pediatric patients with ANCA-positive glomerulonephritis initially presenting with hematuria, mild proteinuria, and normal renal function. The first patient with a high myeloperoxidase-ANCA titer (>134 IU/mL) was diagnosed with rapidly progressive glomerulonephritis based on renal biopsy and treated with immunosuppressive therapy after 10 months of follow-up. The second patient with a low myeloperoxidase-ANCA titer (11 IU/mL) maintained normal kidney function without medication. Two cases showed different clinical course according to ANCA titer.

• 한국미생물·생명공학회지
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• 제33권2호
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• pp.148-153
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• 2005

말라리아는 세계적으로 다시 발생하는 감염성 질환으로 모기에 의해 옮겨지는 말라리아 원충에 의해서 발병한다. 말라리아 원충을 검출하기 위하여 혈액 도말법 등 여러 가지 정량적인 assay가 활용 되고 있다. Real time PCR 은 반응이 일어나는 동안 PCR 산물의 생성을 계속적으로 모니터링 할 수 있는 방법으로서 최근 많은 환자들의 말라리아 원충을 한번에 탐지 하는데 적용되고 있다. 본 연구에서는 말라리아 원충 중 한국에서 질환을 일으키는 Plasmodium vivax를 탐지하기 위해 26명의 환자 혈액에서 분리 정제한 genomic DNA를 가지고 SYBR Green based-real time PCR을 수행하였다. 특히, 기존의 real time PCR에 사용한 18S rRNA와는 달리 DBP 유전자를 사용하여 새로운 말라리아 검출방법을 정량적이면서도 간편하고 경제적인 방법으로 개발하였다. 특히, real time PCR로 나온 결과를 임상적인 titer로 바꾸어 주기 위해서 reference 유전자로는 말라리아 환자의 상태와 관계없이 ACTB이 안정하다는 것을 real time PCR로 입증하였고 ACTB 유전자를 reference 유전자로 사용하였다. 본 연구에서는 real time PCR assay에 DBP라는 유전자를 처음 사용하였을 뿐 아니라 titer 보정을 위한 reference 유전자, ACTB를 real time PCR assay을 통해 확보하여 더 정확한 titer 값을 얻고자 했다. 이런 결과를 바탕으로 Taqman based-real time PCR과 본 연구의 결과를 정량비교를 하였다. 특히, 26명의 말라리아 환자 샘플을 3 group(Group I, II, III)로 나누어서 그 결과 정량적인 경향성 일치를 나타내었다. 특히, 높은 titer을 갖는 말라리아 샘플(Group I)에서 가장 많은 정량적인 차이를 보였지만, 간편하고 경제적인 SYBR Green-based real time PCR을 이용하여 DBP 유전자를 증폭하는 새로운 방법으로 말라리아를 Semi-quantitative 하게 검출할 수 있음을 보였다.

• 한국식품영양과학회지
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• 제30권6호
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• pp.1283-1286
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• 2001

마크로파지의 활성을 조사하기 위해 carbon clearance time을 조사한 결과 AEY 처리구가 control이나 CEY 처리구에 비해 짧았다. 특히 AEY 500$\mu\textrm{g}$ 처리에서 carbon clearance 시간이 5분으로 control에서 9.42분, CEY(250$\mu\textrm{g}$ 처리)에서 9.01분보다 유의성 있는 감소를 보였다. AEY 처리에 의해 응집소가와 용혈소가가 다소증가되었다. SRBC처리 1일째와 3일째에 250$\mu\textrm{g}$ 처리구를 비교해 보면, control, CEY, AEY의 응집소가는 각각 5.50, 5.63, 6.00 및 5.25, 5.38, 5.50로 용혈소가는 각각 4.75, 5.38, 5.50 및 4.25, 5.63, 5.63으로 AEY 처리구가 control, CEY 처리구에 비해 면역 활성이 있었지만 유의성은 없었다.

• Environmental Analysis Health and Toxicology
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• 제1권1호
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• pp.47-54
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• 1986

The immunopotentiating effect of ethanol extract, butanol fraction and petroleum ether extract of Panax ginseng on the immunotoxicity of benzo(a)pyrene were investigated in mice. A single administration of benzo(a)pyrene induced an apparent but relatively transient reduction in HY titer, Arthus reaction, delayed type hypersensitivity, rosette forming cell and natural killer cell activity Ethanol extract very significantly restored HY titer, Arthus reaction. RFC and natural killer cell activity. Butanol fraction have no effect. But petroleum ether extract very significantly restored humoral and cellular immune response and especially natural killer cell activity.

• 대한수의학회지
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• 제10권2호
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• pp.7-12
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• 1970

This study was conducted to abserve the effects of cortisone acetate, estrogenic hormone and tocopherol on the antibody tresponse to both pullorum suspects and negatives of The increase of antibody titer was shown in a few birds after the treatment of either cortisone acetate or estrogenic hormone. There wuas no change in antibody titer of chickens treated with tocopherol.

• 한국동물위생학회지
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• 제14권1호
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• pp.19-26
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• 1991

A considerably high rate of abnormal deliveries of unknown etiology was observed among dairy cattles from November 1988 to February 1989 and Korean native tattles from January to April 1990. The abnormal deliveries consisted of abortions, stillbirths and calf deformities refers to as congenital arthrogryposis hydranencephaly (AH )syndrome. In order to know the level of Akabane antibody of dairy cattle raised in Kyungbuk province, serum neutralization test was conducted with Akabane virus(OBE-1 strain) and HmLu(Hamster lung) cell line. The results were summarized as follows. 1. During 4 months(Nov. 1988-Feb. 1989), abortion (3 heads), stillbirth(1 head) and congenital abnormalities(13 heads) of newborn were occurred in 17 dairy cattles raised in Kyungbuk province. 2. During 4 months(Jan.-Apr.1990), stillbirth(2 heads) and congenital deformities (13 heads) of newborn were occurred in 15 Korean native tattles raised in Kyungbuk province. 3. In Fev, and Apr. 1990, 1,005 dairy cattles at 99 farms were investigated on the actual condition of possessing Akabane antibody. The result was that 1,000 heads (99.9%) in 1,005 dairy cattles reacted as positive condition in Akahane antibody. The antibody titer was from 4 to over 256. 4. 189 heads (18.8%) of 1,005 dairy cattles werw below antibody titer 8 and 816 heads (81.2%) were over 16. 5. Akabane antibody titer of east coast legions(Pohang Yeongil etc) was all over 16, that of internal legions (Yeongiu, Andong. etc) was relatively low, The result suggest that the vaccination for Akabane disease will be unnecessary for the time being because of possessing higher antibody titer reaction except the newly introduced cattle and Akabane virus was widely disseminated in kyungbuk province during the summer months in 1987 or 1988.

• Annals of Clinical Neurophysiology
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• 제3권1호
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• pp.9-14
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• 2001

Background : Bell's palsy(BP) is defined as an idiopathic peripheral facial paralysis of acute onset, accounting for more than 50% of all cases of facial paralysis. Different theories on the etiology of BP have been proposed. Herpes simplex virus-1(HSV) has been the most suspicious causative agent, but varicella zoster virus(VZV) also is suspected. Objectives : We evaluated the serological changes of IgG and IgM titer of HSV and VZV to know the causative agent of BP. Materials and Methods : Subjects consisted of 35 patients who developed acute idiopathic unilateral facial palsy(16 men and 19 women from 9 to 78 years old) within a week of onset. We took the serum of the acute and convalescent stages, respectively. Serum IgG and IgM titer of HSV and VZV were measured in acute and convalescent stages by EIA method. Results : Only the HSV IgG titer showed statistically significant elevation in the convalescent stage(p=0.0291). Others did not show any significant change between the acute and convalescent stage. Conclusion : We concluded that HSV may be related to the causative agent of BP.

• 대한수의학회지
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• 제30권3호
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• pp.297-307
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• 1990

To establish an agar-gel immunodiffusion (AGID) test for detection of antibodies to Aujeszky's disease virus(ADV) in swine, the precipitating antigens were prepared by four procedures using the Aujeszky's disease virus, NYJ-1-87 strain isolated from the affected piglets in Korea. The optimal condition for AGID test and the properties of the antigens were investigated. To determine the optimal concentration of antigens, four antigens were experimentally prepared by concentrating the viral fluids by 1/30 to 1/200. It was proved that the antigen precipitated with ammonium sulfate at concentration of 1/100 was the most efficient to detect ADV antibodies by AGID test. When the relationship between the concentration of the antigens and the size of precipitating in radial immunodiffusion test was investigated, a high correlation coefficiency at r=0.95 (y=0.23x+23.4) was estimated, In study on the effects of various buffered salt solutions and agars on the sensitivity of AGID test by using the experimental ADV antigens, it was found that 0.05M tris buffer without sodium chloride at pH 7.2 induced the most distinctive precipitating lines, and that there was no significant differences in the sensitivity between the agarose and Noble's special agar. When the efficiency of AGID test was compared with serum neutralization(SN) test, the sensitivity of AGID test was 100% in SN titer over 1 : 16, 91.7% in SN titer of 1 : 8 and 57.1% in SN titer of 1 : 4. The specificity of AGID test compared with the sera with SN titer under 1 : 2 was 98.4%. Protein analysis of the antigens by SDS-PAGE indicated that antigen I and antigen III showed a specific band of polypeptides with molecular weight of 116 K in comparison with the control antigen. Antigen IV, treated with tween-80 and ammonium sulfate, revealed specific polypeptides bands at the molecular weights 45K, 98K and 150 K.

• 대한한방종양학회지
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• 제6권1호
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• pp.47-65
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• 2000

In order to investigate antitumor and immune response effect by Hyangsapyungwisan after Sarcoma-180 cells and methotrexate were treatred each other, the extract of Hyangsapyungwisan was orally administered to ICR mice for 14 days. To evaluate the effects of the Hyangsapyungwisan, 50% inhibition concentration($IC_{50}$), mean survival days, tumor weight for antitumor effects, hemagglutinin titer, hemolysin titer, rosette forming cells, natural killer cell activity and productivity of interleukin-2 for immune responses measured in ICR mice. The results were summarized as follows: 1. Mean survival time in Hyangsapyungwisan-treated group was slightly prolonged, as compared with control group(13.46%). 2. On the MTT assay, cell viability was significantly inhibited by $5{\mu}g/well,\;2.5{\mu}g/well,\;1.25{\mu}g/well,\;and\;0.625{\mu}g/well$ of Hyangsapyung-wisan concentration inhibited cell viability significantly. $IC_{50}$ for cell viability was $11.59{\mu}g/well$. 3. Tumor weight in Hyangsapyungwisan treated group was depressed, as compared with the control group(p<0.05). 4. Hemagglutinin titer in Hyangsapyungwisan-treated group was slightly increased with no significance, as compared with the control group. 5. Hemolysin titer in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(p<0.05). 6. Rosette forming cells in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(p<0.05). 7. Naural killer cell activity in Hyangsapyungwisan-treated group was significantly increased(p<0.05). 8. Production of interleukin-2 was significantly increased(p<0.05). According to the above results, Hyangsapygwisan had prominent antitumor effects, and enhance both cellular and humoral immunity in mice.