• Maxillofacial Plastic and Reconstructive Surgery
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• 제41권
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• pp.31.1-31.7
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• 2019

Background: To test the hypothesis that in profile smiling view, for ideal aesthetics, a tangent to the labial face of the maxillary central incisor crowns should be approximately parallel to the true vertical line and thereby perpendicular to the true horizontal line. Methods: An idealized female image was created with computer software and manipulated using the same software to construct an "ideal" female profile image with proportions, and linear and angular soft tissue measurements, based on currently accepted criteria for idealized Caucasian profiles. The maxillary incisor labial face tangent was altered in 5° increments from 70 to 120°, creating a range of images, shown in random order to 70 observers (56 lay people and 14 clinicians), who ranked the images from the most to the least attractive. The main outcome was the preference ranks of image attractiveness given by the observers. Results: The most attractive inclination of a tangent to the labial face of the maxillary incisor crowns in profile view in relation to the true horizontal line was 85°, i.e. 5° retroclined from a perpendicular 90° inclination. The most attractive range appears to be between 80 and 90°. Excessive proclination appeared to be less desirable than retroclination. Beyond 105° most observers recommend treatment. Conclusion: In natural head position, the ideal inclination of the maxillary incisor crown labial face tangent in profile view will be approximately parallel to the true vertical line and thereby approximately perpendicular to the true horizontal line.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제30권4호
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• pp.308-315
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• 2004

Aim: The aim of this study was to investigate the mechanism of promoted skin wound healing in skin defects with primary cultured oral mucosal keratinocytes. Materials and methods: Thirty adult female nude mice weighing $20{\pm}2g$ were used for the experiment. Primary cultured and suspended oral mucosal keratinocytes, labeled with BrdU, were scattered onto $1.5cm{\times}1.5cm$ sized full thickness skin defects in the experimental group(N=15), and no grafts were placed the control group(N=15). They were sacrificed at 3 days, 1 week and 2 weeks after the treatment respectively. Histological examination of each wounds were performed to review the healing progress on measuring the length from the wound margin to regenerating epithelial front. The role of keratinocytes were assessed by double immunohistochemical staining with Anti-BrdU and Anti-cytokeratin AE1/3. Results: In the experimental group the wound was completely covered with regenerating epithelia in 2 weeks, but partially regenerated in the control group. The immunohistochemical studies unexpectedly reveal that most of regenerating epithelial cells were induced from marginal epithelium of the margin, not from the scattered keratinocytes. Conclusion: We could successfully confirm that graft of primary cultured oral mucosal keratinocytes promotes the regeneration of skin defects.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제39권2호
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• pp.63-70
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• 2013

Objectives: The purpose of this study was to investigate the wound healing effect of primary cultured oral mucosal keratinocytes (OMKs) and to assess their roles in skin wounds. Materials and Methods: OMK labeled with BromodeoxyUridine were scattered onto $1.5{\times}1.5$ cm skin defects of adult female nude mice (OMK group, n=15). For the control, culture media were placed on the wound (control group, n=15). Mice in both groups were sacrificed at three days (n=5), one week (n=5), and two weeks (n=5), and histomorphometric and immunoblot analyses with keratinocyte growth factor (KGF), interleukin (IL)-6, and IL-$1{\alpha}$ antibody were performed for the biopsied wound specimen. To verify the effect of the cytokine, rhIL-$1{\alpha}$ was applied instead of OMK transplantation, and the OMK and control groups were compared with regard to re-epithelialization. Results: Histomorphometric analyses demonstrated faster re-epithelialization in the graft group than in the control group at the third day, first week, and second week. Newly forming epithelium showed maintenance of the histological character of the skin epithelium. The graft group showed superior expression of KGF, IL-6, and IL-$1{\alpha}$ protein, compared with the control group. Similar faster re-epithelialization was observed after treatment with rhIL-$1{\alpha}$ instead of OMK transplantation. Conclusion: We successfully confirmed that the graft of primary cultured OMKs promoted regeneration of skin defects. The mechanism of accelerated wound healing by primary cultured OMKs was attributed to inducement of cytokine expression as required for re-epithelialization.

• Asian Pacific Journal of Cancer Prevention
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• 제16권2호
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• pp.571-578
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• 2015

Prostate cancer is the most common cancer in men. In this study, we investigated immune responses of cytotoxic T lymphocytes (CTLs) against TRAMP-C2 prostate cancer cells after activation by dendritic cells (DCs) loaded with TRAMP-C2 freeze-thaw antigen and/or PEP-3 peptide in vitro. Bone marrow-derived DC from the bone marrow of the C57BL/6 were induced to mature by using the cytokine of rhGM-CSF and rhIL-4, and loaded with either the freeze-thaw antigen or PEP-3 peptide or both of them. Maturation of DCs was detected by flow cytometry. The killing efficiency of the CTLs on TRAMP-C2 cells were detected by flow cytometry, CCK8, colony formation, transwell migration, and wound-healing assay. The levels of the IFN-${\gamma}$, TNF-${\beta}$ and IL-12 were measured by enzyme-linked immunosorbent assay (ELISA). Compared with the unloaded DCs, the loaded DCs had significantly increased expression of several phenotypes related to DC maturation. CTLs activated by DCs loaded with freeze-thaw antigen and PEP-3 peptide had more evident cytotoxicity against TRAMP-C2 cells in vitro. The secretion levels of IFN-${\gamma}$, TNF-${\beta}$ and IL-12, secreted by DCs loaded with antigen and PEP-3 and interaction with T cells, were higher than in the other groups. Our results suggest that the CTLs activated by DCs loaded with TRAMP-C2 freeze-thaw antigen and PEP-3 peptide exert a remarkable killing efficiency against TRAMP-C2 cells in vitro.

• 전자공학회논문지
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• 제51권2호
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• pp.173-181
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• 2014

본 논문은 흉부 CT에서 폐, 기관지 및 폐혈관을 자동으로 분할 할 수 있는 알고리즘을 제안 하였다. 제안된 방법은 3단계로 진행된다. 첫째는 최적 임계값과 3차원 레이블링을 통한 영역성장법으로 폐 및 기관지를 분할한다. 둘째는 기관지의 형태학적 정보를 활용하여 기관지의 첫 번째 분기점(용골)까지는 차감연산으로, 이후부터는 가변적 임계값 기법을 적용하여 기관지를 분할한다. 셋째는 폐에 대한 복원 과정으로 좌/우측 폐를 분리하고, 개선된 롤링 볼 알고리즘을 적용하여 폐 외곽의 이상 유무를 확인하며, 이상이 발견되면 그 부분을 제거하고, 2차 다항식 형태로 폐 외곽을 연결시킴으로서 정상적인 폐로 복원한다. 마지막으로 폐혈관은 임계 값 기법의 3 차원 레이블링과 3 차원 영역성장법을 적용하여 분할하였다. 시뮬레이션 결과 폐 주변조직의 손실 없이 정확하게 분할됨을 확인 할 수 있었다.

• Journal of Radiation Protection and Research
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• 제11권1호
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• pp.37-43
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• 1986

ICRU 구(球)를 피사체(被射體)로 하여 300 keV 중성자(中性子)의 방사선량(放射線量) 관계량(關係量)을 평가(評價)하였다. 피사체내(被射體內)의 선량당량(線量當量) 분포(分布)를 직접(直接) 산정(算定)하기 위해 중성자(中性子)-광자(光子)-하전입자(荷電粒子) 결합수송(結合輸送)을 다룰 수 있는 몬테칼로 코드 NEDEP을 사용하였다. 계산결과(計算結果) 얻은 방사선량(放射線量) 관계량(關係量)은 다음과 같다. 심부선량당량지수(深部線量當量指數) $H_{I,d}:1.78{\times}10^{11}\;Sv-cm^2$ 표층선량당량지수(表層線量當量指數) $H_{I,s}:2.08{\times}10^{-1}\;Sv-cm^2$ 주위선량당량(周圍線量當量) $H^*(0.07):1.70{\times}10^{-11}\;Sv-cm^2$ 주위선량당량(周圍線量當量) $H^*(10):1.78{\times}10^{-11}\;Sv-cm^2$ 실효선질계수(實效線質係數) $\bar{Q}^*(10):12.4$

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권6호
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• pp.340-346
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• 2002

The purpose of the present study was to examine the efficacy and mechanism of the PAB (para-amino benzamidine) affinity column chromatography, Viresolve NFP virus filtration, pasteurization (60$\^{C}$ heat treatment for 10 h), and lyophilization steps employed in the manufacture of urokinase from human urine as regards the removal and/or inactivation of the hepatitis A virus (HAV). Samples from the relevant stages of the production process were spiked with HAV and subjected to scale-down processes mimicking the manufacture of urokinase Samples were collected at each step, immediately titrated using a 50% tissue culture infectious dose (TCID$\_$50/), and the virus reduction factors evaluated. PAB chromatography was found to be an effective step for removing HAV with a log reduction factor of 3.24. HAV infectivity was rarely detected in the urokinase fraction, while most of the HAV infectivity was recovered in the unbound and wash fractions. HAV was completely removed during the Viresolve NFP filtration with a log reduction factor of $\geq$ 4.60. Pasteurization was also found to be an effective step in inactivating HAV where the titers were reduced from an initial titer of 7.18 log$\_$10/ TCID$\_$50/ to undetectable levels within 10 h of treatment. The log reduction factor achieved during pasteurization was $\geq$ 4.76. Lyophilization revealed the lowest efficacy for inactivating HAV with a log reduction factor of 1.48. The cumulative log reduction factor was $\geq$ 14.08. Accordingly, these results indicate that the production process for urokinase exhibited a sufficient HAV reducing capacity to achieve a high margin of virus safety.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.429-432
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• 2000

생체 적합성, 생분해성, 항균성 등의 특징을 갖는 키토산 지지체는 type I -p collagen과 bFGF 또는 fibronectin을 함께 코팅함으로써 세포적합성을 향상시켜 섬유아세포의 증식과 ECM의 분비를 증가시킬 수 있으며, 인공피부를 위한 적합한 지지체로 사용될 수 있다고 사료된다.

• 한국지능시스템학회논문지
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• 제14권4호
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• pp.411-417
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• 2004

최근 바이오 관련산업의 발전과 함께 바이오 장비 및 장치들에 대한 연구 및 개발이 활발하게 진행되고 있다. 특히 바이오 세포 조작관련 연구들이 많이 진행되어 오고 있다. 일반적으로 바이오 세포들에 대해 기계적인 엔드 이펙터들이 조작을 위해 접촉될 때 과도한 힘이 발생될 경우가 발생하며 이런 힘들에 의해 세포막이나 조직들이 피해를 입을 수 있다. 본 논문에서는 상기 문제들을 극복하기 위해 바이오 세포 조작을 위한 새로운 시스템을 제안하였다. 제안된 시스템은 내장된 힘 센서를 이용하여 바이오 세포와 엔드 이펙터간의 발생 힘을 측정할 수 있다. 또한, 비전기술을 이용하여 엔드 이펙터의 피펫 팀을 바이오 세포막까지 정확하게 가이드 할 수 있다. 결과적으로 제안된 시스템은 바이오 세포에 피해를 주지 않고 안전하게 조작이 가능하다. 제안된 기술을 이용하여 실제 시작품을 제작하여 다양한 실험을 수행한 결과 향후 DNA 조작과 같은 바이오 세포 조작용 정밀 인젝션 시스템으로의 사용 가능성을 보여 주었다.

• BMB Reports
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• 제42권6호
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• pp.344-349
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• 2009

Angiogenesis is crucial for solid tumor growth. By secreting angiogenic factors, tumor cells induce angiogenesis. However, targeting these angiogenic factors for cancer therapy is not always successful, suggesting that other factors may be involved in tumor angiogenesis. This work shows that 25 protein spots were differentially expressed by two-dimensional gel electrophoretic analysis when HepG2 cells induced endothelial cell differentiation to tube in vitro, and most of them were upregulated. Twenty-one proteins were identified with MALDITOF-MS, and the other four were identified by LTQ-MS/MS. Keratins were identified as one class of these upregulated proteins. Further study indicated that the expression of keratin 17 in cultured endothelial cells is likely microenvironment regulated, because its expression can be induced by HepG2 cells and bFGF as well as serum in culture media. Increased expression of keratins in endothelial cells, such as keratin 17, may contribute to the angiogenesis induced by HepG2 cells.