• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.9
• /
• pp.559-568
• /
• 2020

Epidermal growth factor (EGF) is a hormone protein that affects cell growth and proliferation, and has various medical applications. In the present study, the gene of human EGF was codon-optimized with E. coli and the expression vector was constructed by cloning into pRSET. In order to obtain the recombinant human EGF in an active form rather than an inclusion body, chaperone co-expression was attempted along with codon optimization, for the first time. The expressed human EGF was isolated in the pure form by performing Ion Exchange Chromatography in two consecutive runs. ELISA analysis showed that the activity of purified EGF was greater than 99%, which is similar to commercially available EGF. Cell proliferation test confirmed that the recombinant human EGF has the ability to promote cell proliferation of human skin fibroblasts. The human EGF expression system of this study gives a significant amount of protein, and does not require the renaturation step and the additional chromatographic system to remove a fusion contaminant, thereby providing a very useful alternative to conventional expression systems for the preparation of recombinant human EGF.

• Journal of the Institute of Convergence Signal Processing
• /
• v.4 no.1
• /
• pp.15-26
• /
• 2003

Ultrasonic inspection methods are widely used for detecting flaws in materials. The signal analysis step plays a crucial part in the data interpretation process. A number of signal processing methods have been proposed to classify ultrasonic flaw signals. One of the more popular methods involves the extraction of an appropriate set of features followed by the use of a neural network for the classification of the signals in the feature space. This paper describes an alternative approach which uses the least mean square (LMS) method and expectation maximization (EM) algorithm with the model based deconvolution which is employed for classifying nondestructive evaluation (NDE) signals from steam generator tubes in a nuclear power plant. The signals due to cracks and deposits are not significantly different. These signals must be discriminated to prevent from happening a huge disaster such as contamination of water or explosion. A model based deconvolution has been described to facilitate comparison of classification results. The method uses the space alternating generalized expectation maximization (SAGE) algorithm In conjunction with the Newton-Raphson method which uses the Hessian parameter resulting in fast convergence to estimate the time of flight and the distance between the tube wall and the ultrasonic sensor Results using these schemes for the classification of ultrasonic signals from cracks and deposits within steam generator tubes are presented and showed a reasonable performances.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.25 no.1
• /
• pp.27-35
• /
• 2005

Ultrasonic inspection methods are widely used for detecting flaws in materials. The signal analysis step plays a crucial part in the data interpretation process. A number of signal processing methods have been proposed to classify ultrasonic flaw signals. One of the more popular methods involves the extraction of an appropriate set of features followed by the use of a neural network for the classification of the signals in the feature spare. This paper describes an alternative approach which uses the least mean square (LMS) method and exportation maximization (EM) algorithm with the model based deconvolution which is employed for classifying nondestructive evaluation (NDE) signals from steam generator tubes in a nuclear power plant. The signals due to cracks and deposits are not significantly different. These signals must be discriminated to prevent from happening a huge disaster such as contamination of water or explosion. A model based deconvolution has been described to facilitate comparison of classification results. The method uses the space alternating generalized expectation maximiBation (SAGE) algorithm ill conjunction with the Newton-Raphson method which uses the Hessian parameter resulting in fast convergence to estimate the time of flight and the distance between the tube wall and the ultrasonic sensor. Results using these schemes for the classification of ultrasonic signals from cracks and deposits within steam generator tubes are presented and showed a reasonable performances.

• Journal of Korea Water Resources Association
• /
• v.43 no.2
• /
• pp.153-165
• /
• 2010

Large-Scale Particle Image Velocimetry (LSPIV) is an extension of particle image velocimetry (PIV) for measurement of flows spanning large areas in laboratory or field conditions. LSPIV is composed of six elements - seeding, illumination, recording, image transformation, image processing, postprocessing - based on PIV. Possible error elements at each step of Mobile LSPIV (MLSPIV), which is a mobile version of LSPIV, in field applications are identified and summarized the effect of the errors which were quantified in the previous studies. The total number of elemental errors is 27, and five error sources were evaluated previously, seven elemental errors are not effective to the current MLSPIV system. Among 15 elemental errors, four errors - sampling time, image resolution, tracer, and wind - are investigated through an experiment at a laboratory to figure out how those errors affect to velocity calculation. The analysis to figure out the effect of the number of images used for image processing on the velocity calculation error shows that if over 50 images or more are used, the error due to it goes below 1 %. The effect of the image resolution on velocity calculation was investigated through various image resolution using digital camera. Low resolution image set made 3 % of velocity calculation error comparing with high resolution image set as a reference. For the effect of tracers and wind, the wind effect on tracer is decreasing remarkably with increasing the flume bulk velocity. To minimize the velocity evaluation error due to wind, tracers with high specific gravity is favorable.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.18 no.2
• /
• pp.117-125
• /
• 2014

This paper examines the fundamental properties of alkali-activated slag cement (AASC) activated by sodium hydroxide (NaOH). The water to binder (W/B) ratio was 0.4 and 0.5. And concentration of activator were 2M and 4M. Five mix design of each W/B ratios was considered. The N0 mixture was KS L 5109 method and N1~N4 were varied in different mixing time, mix step and entering points of fine aggregate. Test results clearly showed that the flow value, strength and drying shrinkage development of AASC were significantly dependent on the entering point of fine aggregate. The flow value tended to decreases with delaying entering point of fine aggregate. The compressive strength and flexural strength increases with delaying entering point. Moreover, the XRD analysis confirmed that there were sustain these results. The drying shrinkage increases with delaying entering point of fine aggregate. Futhermore, a modified mixing method incorporating all hereby experimentally derived parameters, is proposed to improvement the physical properties of AASC.

• Journal of Radiation Protection and Research
• /
• v.30 no.3
• /
• pp.99-105
• /
• 2005

A model for evaluating radioactive contamination in the urban environment, named METRO-K, was developed as a basic step for accident consequence analysis in case of an accidental release. The three kind of radionuclides $(^{137}Cs,\;^{106}Ru,\;^{131}I)$ and the different chemical forms of iodine (particulate, organic and elemental forms) are considered in the model. The radioactive concentrations are evaluated for the five types of surface (roof, paved road, wall, lawn/soil, tree) as a function of time. Using the model, the contaminative impacts of the surfaces were intensively investigated with respect to with and without precipitation during the measurement periods of radionuclides in air. In addition, a practical application study was conducted using $^{137}Cs$ concentration in air and precipitation measured in an European country at the Chernobyl accident. As a result precipitation was an influential factor in surface contamination. The degree of contamination was strongly dependent on the types of radionuclide and surface. Precipitation was more influential in contamination of $^{137}Cs$ than that of $^{131}I$ (elemental form).

• Journal of the Society of Naval Architects of Korea
• /
• v.30 no.1
• /
• pp.65-72
• /
• 1993

A numerical method for nonlinear free-surface-wave problem is developed in this paper. The final goal of this study is to simulate the towing tank experiment of a ship model and to partially replace the experiment by the numerical model. The exact problem in the scope of potential flow theory is formulated by a variational principle based on the classical Hamilton's principle. A localized finite element method is used in the present numerical computations which made use of the following two notable steps. The first step is an efficient treatment of the numerical radiation condition by using the intermediate nonlinear-to-linear transition buffer subdomain between the fully nonlinear and linear subdomains. The second is the use of a modal analysis in the final stage of the solution procedures, which enables us to reduce the computation time drastically. With these improvements the present method can treat a much larger computational domain than that was possible previously. A pressure patch on the free surface was chosen as an example. From the present computed results we could investigate the effect of nonlinearity on the down-stream wave pattern more clearly than others, because much larger computational domain was treated. We found, specifically, the widening of the Kelvin angle and the increase of the wave numbers and the magnitude of wave profiles.

• The Mathematical Education
• /
• v.27 no.1
• /
• pp.15-23
• /
• 1988

We are at the onset of a major revolution in education, a revolution unparalleled since the invention of the printing press. The computer will be the instrument of this revolution. Computers and computer application are everywhere these days. Everyone can't avoid the influence of the computer in today's world. The computer is no longer a magical, unfamiliar tool that is used only by researchers or scholars or scientists. The computer helps us do our jobs and even routine tasks more effectively and efficiently. More importantly, it gives us power never before available to solve complex problems. Mathematics instruction in secondary schools is frequently perceived to be more a amendable to the use of computers than are other areas of the school curriculum. This is based on the perception of mathematics as a subject with clearly defined objectives and outcomes that can be reliably measured by devices readily at hand or easily constructed by teachers or researchers. Because of this reason, the first large-scale computerized curriculum projects were in mathematics, and the first educational computer games were mathematics games. And now, the entire mathematics curriculum appears to be the first of the traditional school curriculum areas to be undergoing substantial trasformation because of computers. Recently, many research-Institutes of our country are going to study on computers in orders to use it in mathematics education, but the study is still start ing-step. In order to keep abreast of this trend necessity, and to enhance mathematics teaching/learning which is instructed lecture-based teaching/learning at the present time, this study aims to develop/present practical method of computer-using. This is devided into three methods. 1. Programming teaching/learning method This part is presented the following five types which can teach/learn the mathematical concepts and principle through concise program. (Type 1) Complete a program. (Type 2) Know the given program's content and predict the output. (Type 3) Write a program of the given flow-chart and solve the problem. (Type 4) Make an inference from an error message, find errors and correct them. (Type 5) Investigate complex mathematical fact through program and annotate a program. 2. Problem-solving teaching/learning method solving This part is illustrated how a computer can be used as a tool to help students solve realistic mathematical problems while simultaneously reinforcing their understanding of problem-solving processes. Here, four different problems are presented. For each problem, a four-stage problem-solving model of polya is given: Problem statement, Problem analysis, Computer program, and Looking back/Looking ahead. 3. CAI program teaching/learning method This part is developed/presented courseware of sine theorem section (Mathematics I for high school) in order to avail individualized learning or interactive learning with teacher. (Appendix I, II)

• Land and Housing Review
• /
• v.4 no.4
• /
• pp.383-393
• /
• 2013

The trends of green growth and eco-friendliness came to be the core development indicator for the sustainable global environment. Korean government reflected these trends in the main flows of the national development index, and suggested diverse directions for green construction technologies and high quality construction environment through Third master plan for construction environment. However, the efforts to follow these trends during the construction process as a step for production phrase are not being considered enough yet. In this study, we identified the basic environmental management factors in order to enhance the eco-friendliness of public housing construction sites, and suggested the reasonable conducting parties and process for those respective factors. The results of this study are expected to be the valuable reference in defining the required activities and participants' responsibilities, and improving the work process for systematic on-site environmental management. In applying those results, the discussion should be followed on the executing party of each unit activity and the responsibility assignment for each process. At the same time, the legislation and standard related to environment need to be essentially amended. In the future, the method of evaluating the environmental management activities, and the technical solution to environmental problems are to be reviewed as a further research for successful environmental management.

• The Journal of the Korea Contents Association
• /
• v.18 no.6
• /
• pp.63-72
• /
• 2018

This study was conducted to explore the impact of learning strategies on the level of participation in the online collaborative learning process. To do this, We studied 91 students who took professorship courses at A university in the Seoul metropolitan area. At the beginning of the semester, the learning strategies were measured through MSLQ, rehearsal, elaboration, organizing, critical thinking, metacognition, learning management, effort control, peer learning, and seeking help. Next, cooperative tasks were carried out to measure the interaction, and group composition consisted of 4-5 persons. The level of participation was measured by scores given to the messages created for interaction. The process of collaborative learning was divided into the steps of identifying learning goals, learning plans, performing individual learning, sharing learning results, and writing reports. The effects of learning strategies on participation level were analyzed through multiple regression analysis (stepwise selection method). As a result, the learning goal step influenced the highest level of metacognition, and the learning plan is the management of the learning time, the demonstration of the learning execution, the adjustment of the effort, the acquisition of help, the collegial learning, Writing was influenced by organization, elaboration, critical thinking, and critical thinking, metacognition, and elaboration.