Our previous studies clearly demonstrated that a combination of WP 631 and Epo B has higher activity against ovarian cancer cells than either of these compounds used separately. In order to fully understand the exact mechanism of action in combination, we assessed effects on the cell cycle of SKOV-3 cells. We evaluated three control points essential for WP 631 and Epo B action to determine which cell cycle-regulating proteins (CDK1/cyclin B complex, EpCAM or HMGB1) mediate activity. The effects of the drug on the cell cycle were measured based on the nuclear DNA content using flow cytometry. Expression of cell cycle-regulating genes was analyzed using real-time PCR. It was discovered that WP 631, at the tested concentration, did not affect the SKOV-3 cell cycle. Epo B caused significant G2/M arrest, whereas the drug combination induced stronger apoptosis and lower mitotic arrest than Epo B alone. This is very important information from the point of view of the fight against cancer, as, while mitotic arrest in Epo B-treated cells could be overcame after DNA damage repair, apoptosis which occurs after mitotic slippage in combination-treated cells is irreversible. It clearly explains the higher activity of the drug combination in comparison to Epo B alone. Epo B acts via the CDK1/cyclin B complex and has the ability to inhibit CDK1, which may be a promising strategy for ovarian cancer treatment in the future. The drug combination diminishes EpCAM and HMGB1 expression to a greater degree than either WP 631 and Epo B alone. Owing to the fact that the high expression of these two proteins is a poor prognostic factor for ovarian cancer, a decrease in their expression, observed in our studies, may result in improved efficacy of cancer therapy. The presented findings show that the combination of WP 631 and Epo B is a better therapeutic option than either of these drugs alone.
Huifang Li;Mi Zhao;Jingqi Huang;Weizhang Liao;Chao Ma
Earthquakes and Structures
/
v.24
no.1
/
pp.65-79
/
2023
A simplified analytical solution for seismic response of tunnel cross section in horizontally layered ground subjected to oblique incidence of SH wave is deduced in this paper. The proposed analytical solution consists of two main steps: free-field response in layered field and tunnel response. The free field responses of the layered ground are obtained by one-dimensional finite element method in time domain. The tunnel lining is treated as a thick-wall cylinder to calculate the tunnel response, which subject to free field stress. The analytical solutions are verified by comparing with the dynamic numerical results of two-dimensional ground-lining interaction analysis under earthquake in some common situations, which have a good agreement. Then, the appropriate range of the proposed analytical solution is analyzed, considering the height of the layered ground, the wavelength and incident angle of SH wave. Finally, by using the analytical solutions, the effects of the ground material, burial depth of the tunnel, and lining thickness and the slippage effect at the ground-lining interface on the seismic response of tunnels are investigated. The proposed solution could serve as a useful tool for seismic analysis and design of tunnels in layered ground.
This study is to present more effective starting skills through analysis of kinematic characteristics of starting motions in 300m Time Trials of Inline Skating. To achieve this goal, 6 athletes, 3 in the national team and 3 in business teams were chosen and their starting motions were examined with three-dimensional image analyses. The results of analyses in regard of positions and speed of the bodily center and angles, angular velocity, and linear velocity of articulations of lower limbs by sections of starting motions are as follows: In case of the central position, though it is effective to reduce the air resistance by lowering the upper part of the body maximumly, it is reasonable to accelerate by raising the upper part of the body to some degree for the running posture at the lower speed in the starting section. In the starting section, it is efficient to minimize the period of time in touching the ground. for this, it is necessary to train for taking motions without slippage while touching the ground. While 3 athletes in business teams kicked the ground as running right after the starting, the others in the national team slid on the ground. As the number of steps increased, the movable speed changed quickly. Thus the movable speed of athletes in the national team indicated big differences in two to three steps. If these factors are well supported, the push-away starting method might be better than the running starting method in terms of improvement of records.
Since the skid-steer loader is able to work for excavating, lifting and transporting load even at the narrow space, they are widely used in the regular farm and the livestock farm. The skid-steer loader normally adopts the hydrostatic transmission because the power to move the machine backward and forward should be delivered independently on both sides of wheels. Contrast to the mechanical system such as chain and belt transmissions, however, the hydrostatic transmission is less efficient in the use of energy and more difficult in the maintenance. This study was intended to investigate the feasibility of using triangular-type belt clutch and V-belt transmission for the newly developed skid-steer loader in order to overcome the problems stated in the hydrostatic transmission. In the developed triangular-type belt clutch, the centers of driving, driven and idler sheaves are arranged in the triangular shape in a plane, and V-belts were loaded loosely on three sheaves. The power is transmitted by pressing the idler connected to a lever on the loosened V-belt. Contrast to the normal belt clutch using two sheaves, the newly developed belt clutch has the characteristics of small contact-angle of the driving sheave at no bucket load and increasing contact-angle at the time of power transmission. The results of research can be summarized as follows: 1) The developed triangular-type belt clutch adopted a spring-loaded slackside idler which could transmit more power than a fixed idler could by sacrificing the belt life. The life of V-belt used in the power transmission reached at 500 hours(6 months) when the engine power of 11.8 ㎾ was transmitted. Also, it was feasible to develop the large industrial skid-loader with the V-belt transmission by using the proper set of sheaves. 2) The developed skid-steer loader changed the rotating radius and speed with bucket loads as the conventional skid steer loader did. The rotating speed was 47 deg/s at the maximum bucket load of 2.74 kN when the minimum rotating radius was 1.5m. 3) The power required in turning at the bucket load of 2.74 kN was 4 ㎾ and the slippage of V-belt was less than 1%.
Kim, Byung-Ki;Choi, Jun-Gu;Lee, Jun;Lee, Min-Woo;Kim, Sun-Bae;Kim, Gyeong-Su
Korean Journal of Digital Imaging in Medicine
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v.10
no.1
/
pp.13-19
/
2008
Purpose : In sponge quality of the material in digital radiography environment coating because do position blocks two that do not become coating done positioning blocks two that do not become coating done positioning block and sponge quality of the material to testing bench image artifact's difference compare. Method of study : In digital radiography environment positioning block of different two products same sunshine or effect that image and positioning block artifact gents in image analyze. the target used positioning block 2 when examine hand, rib and examination condition did each differently according to used positioning block. reflex compared picture that do image and verify that examine first time. Result : 1. In sponge quality of the material coating in done product artifact appear. Did not appear in product that do not become coating. 2. Can know coating's existence, that artifact according to radish happens. 3. Quality of the material coating's existence, there was difference of slippage according to radish Conclusion : Quality of the material coating phenomenon that done positioning block thus, it may have to be considered that use after estimate degree that get in image.
Khan, Muhammad;Mccrary, Evan;Nnaji, Chukwuma;Awolusi, Ibukun
International conference on construction engineering and project management
/
2022.06a
/
pp.579-586
/
2022
Modular building is a fast-growing construction method, mainly due to its ability to drastically reduce the amount of time it takes to construct a building and produce higher-quality buildings at a more consistent rate. However, while modular construction is relatively safer than traditional construction methods, workers are still exposed to hazards that lead to injuries and fatalities, and these hazards could be controlled using emerging smart technologies. Currently, limited information is available at the intersection of modular construction, safety risk, and smart safety technologies. This paper aims to investigate what aspects of modular construction are most dangerous for its workers, highlight specific risks in its processes, and propose ways to utilize smart technologies to mitigate these safety risks. Findings from the archival analysis of accident reports in Occupational Safety and Health Administration (OSHA) Fatality and Catastrophe Investigation Summaries indicate that 114 significant injuries were reported between 2002 and 2021, of which 67 were fatalities. About 72% of fatalities occurred during the installation phase, while 57% were caused by crushing and 85% of crash-related incidents were caused by jack failure/slippage. IoT-enabled wearable sensing devices, computer vision, smart safety harness, and Augment and Virtual Reality were identified as potential solutions for mitigating identified safety risks. The present study contributes to knowledge by identifying important safety trends, critical safety risk factors and proposing practical emerging methods for controlling these risks.
Cracking is an inevitable fact of asphalt concrete pavements and plays a major role in pavement deterioration. Pavement cracking is one of the main factors determining the frequency and method of repair. Cracks can be treated with a number of preventative maintenance actions, including overlay surface treatments such as slurry sealing, crack sealing, or crack filling. Pavement cracks can show up as one or all of the following types: transverse, longitudinal, fatigue, block, reflective, edge, and slippage. Crack sealing is a frequently used pavement maintenance treatment because it significantly extends the pavement service life. However, crack sealant often fails prematurely due to a loss of adhesion. Because current test methods are mostly empirical and only provide a qualitative measure of the bond strength, they cannot accurately predict the adhesive failure of the sealant. This study introduces a laboratory test aimed at assessing the bonding of hot-poured crack sealant to the walls of pavement cracks. A pneumatic adhesion tensile testing instrument (PATTI) was adopted to measure the bonding strength of the hot-poured crack sealant as a function of the curing time and temperature. Based on a limited number of test results, the hot-poured crack sealants have very different bonding performances. Therefore, this test method can be proposed as part of a newly developed performance-based standard specification for hot-poured crack sealants for use in the future. PURPOSES : The purpose of this study was to evaluate both the adhesion and failure performance of a crack sealant as a function of its curing time and curing temperature. METHODS: A pneumatic adhesion tensile testing instrument (PATTI) was adopted to measure the adhesion performance of a crack sealant as a function of the curing time and curing temperature. RESULTS: With changes in the curing time, curing temperature, and sealant type, the bond strengths were found to be significantly different. Also, higher bond strengths were measured at lower temperatures. Different sealant types produced completely different bond strengths and failure behaviors. CONCLUSIONS: The bonding strength of an evaluated crack sealant was shown to differ depending on various factors. Two sealant types, which were composed of different raw materials, were shown to perform differently. The newly proposed test offers the possibility of evaluating and differentiating between different crack sealants. Based on alimited number of test results, this test method can be proposed as part of a newly developed performance-based standard specification for crack sealants or as part of a guideline for the selection of hot-poured crack sealant in the future.
Purpose: To evaluate the optimal number of additional half hitches for achieving an optimal knot-holding capacity (KHC) of Lockable sliding knots. Methods: Four configurations of arthroscopic knots (Duncan loop, Field knot, Giant knot, and SMC knot) were tested for their knot-holding capacity. For each knot configuration, 6 sequential knots were made including the initial sliding knot and additional 5 knots by incrementing one half hitches at a time. Each added half-hitch were in reversing half-hitches with alternate posts (RHAPs) fashion. For each sequential knot configuration, 12 knots were made by No. 2 braided sutures. On the servo-hydraulic material testing system (Instron 8511, MTS, Minneapolis, MN), cyclic loading, load to clinical failure (3-mm displacement), load to ultimate failure, and mode of failure were measured. Results: Most of the initial loop without additional half-hitch showed dynamic failure with cyclic loading. The mean displacement after the end of cyclic loading decreased with each additional half-hitches. SMC and Giant knot reached plateau to 0.1 mm or less displacement after one additional half-hitch, shereas Field and Duncan loop needed 3 additional half-hitches. The SMC and Duncan knots needed 1 additional half-hitch to reach greater than 80N at clinical failure, whefeas the other 2 knots needed2 additional half-hitches. For the load exceeding 100N for clinical failure, the SMC knot required 3 additional half-hitches and the other three knots needed 4 additional half-hitches. As the number of additional half-hitches incremented, the mode of failure switched from pure loop failure (slippage) to material failure (breakage). Duncan loop showed poor loop security in that even with 5 additional half-hitches, some failed by slippage (17%). On the other hand, after 3 additional half-hitches, the 3 other knots showed greater than 75% of failure by material breakage mode (SMC and Field 92%, Giant 75%). Conclusion: Even with its own locking mechanism, lockable sliding knot alone does not withstand the initial dynamic cyclic load. For all tested variables, SMC knot requires a minimum of 2 additional half-hitches. Duncan knot may need more than 3 additional half-hitches for optimal security. All knots showed a mear plateau in knot security with 3 or more additional half-hitches.
System trading is becoming more popular among Korean traders recently. System traders use automatic order systems based on the system generated buy and sell signals. These signals are generated from the predetermined entry and exit rules that were coded by system traders. Most researches on system trading have focused on designing profitable entry and exit rules using technical indicators. However, market conditions, strategy characteristics, and money management also have influences on the profitability of the system trading. Unexpected price deviations from the predetermined trading rules can incur large losses to system traders. Therefore, most professional traders use strategy portfolios rather than only one strategy. Building a good strategy portfolio is important because trading performance depends on strategy portfolios. Despite of the importance of designing strategy portfolio, rule of thumb methods have been used to select trading strategies. In this study, we propose a SVM-based strategy portfolio management system. SVM were introduced by Vapnik and is known to be effective for data mining area. It can build good portfolios within a very short period of time. Since SVM minimizes structural risks, it is best suitable for the futures trading market in which prices do not move exactly the same as the past. Our system trading strategies include moving-average cross system, MACD cross system, trend-following system, buy dips and sell rallies system, DMI system, Keltner channel system, Bollinger Bands system, and Fibonacci system. These strategies are well known and frequently being used by many professional traders. We program these strategies for generating automated system signals for entry and exit. We propose SVM-based strategies selection system and portfolio construction and order routing system. Strategies selection system is a portfolio training system. It generates training data and makes SVM model using optimal portfolio. We make $m{\times}n$ data matrix by dividing KOSPI 200 index futures data with a same period. Optimal strategy portfolio is derived from analyzing each strategy performance. SVM model is generated based on this data and optimal strategy portfolio. We use 80% of the data for training and the remaining 20% is used for testing the strategy. For training, we select two strategies which show the highest profit in the next day. Selection method 1 selects two strategies and method 2 selects maximum two strategies which show profit more than 0.1 point. We use one-against-all method which has fast processing time. We analyse the daily data of KOSPI 200 index futures contracts from January 1990 to November 2011. Price change rates for 50 days are used as SVM input data. The training period is from January 1990 to March 2007 and the test period is from March 2007 to November 2011. We suggest three benchmark strategies portfolio. BM1 holds two contracts of KOSPI 200 index futures for testing period. BM2 is constructed as two strategies which show the largest cumulative profit during 30 days before testing starts. BM3 has two strategies which show best profits during testing period. Trading cost include brokerage commission cost and slippage cost. The proposed strategy portfolio management system shows profit more than double of the benchmark portfolios. BM1 shows 103.44 point profit, BM2 shows 488.61 point profit, and BM3 shows 502.41 point profit after deducting trading cost. The best benchmark is the portfolio of the two best profit strategies during the test period. The proposed system 1 shows 706.22 point profit and proposed system 2 shows 768.95 point profit after deducting trading cost. The equity curves for the entire period show stable pattern. With higher profit, this suggests a good trading direction for system traders. We can make more stable and more profitable portfolios if we add money management module to the system.
Volatility in the stock market returns is a measure of investment risk. It plays a central role in portfolio optimization, asset pricing and risk management as well as most theoretical financial models. Engle(1982) presented a pioneering paper on the stock market volatility that explains the time-variant characteristics embedded in the stock market return volatility. His model, Autoregressive Conditional Heteroscedasticity (ARCH), was generalized by Bollerslev(1986) as GARCH models. Empirical studies have shown that GARCH models describes well the fat-tailed return distributions and volatility clustering phenomenon appearing in stock prices. The parameters of the GARCH models are generally estimated by the maximum likelihood estimation (MLE) based on the standard normal density. But, since 1987 Black Monday, the stock market prices have become very complex and shown a lot of noisy terms. Recent studies start to apply artificial intelligent approach in estimating the GARCH parameters as a substitute for the MLE. The paper presents SVR-based GARCH process and compares with MLE-based GARCH process to estimate the parameters of GARCH models which are known to well forecast stock market volatility. Kernel functions used in SVR estimation process are linear, polynomial and radial. We analyzed the suggested models with KOSPI 200 Index. This index is constituted by 200 blue chip stocks listed in the Korea Exchange. We sampled KOSPI 200 daily closing values from 2010 to 2015. Sample observations are 1487 days. We used 1187 days to train the suggested GARCH models and the remaining 300 days were used as testing data. First, symmetric and asymmetric GARCH models are estimated by MLE. We forecasted KOSPI 200 Index return volatility and the statistical metric MSE shows better results for the asymmetric GARCH models such as E-GARCH or GJR-GARCH. This is consistent with the documented non-normal return distribution characteristics with fat-tail and leptokurtosis. Compared with MLE estimation process, SVR-based GARCH models outperform the MLE methodology in KOSPI 200 Index return volatility forecasting. Polynomial kernel function shows exceptionally lower forecasting accuracy. We suggested Intelligent Volatility Trading System (IVTS) that utilizes the forecasted volatility results. IVTS entry rules are as follows. If forecasted tomorrow volatility will increase then buy volatility today. If forecasted tomorrow volatility will decrease then sell volatility today. If forecasted volatility direction does not change we hold the existing buy or sell positions. IVTS is assumed to buy and sell historical volatility values. This is somewhat unreal because we cannot trade historical volatility values themselves. But our simulation results are meaningful since the Korea Exchange introduced volatility futures contract that traders can trade since November 2014. The trading systems with SVR-based GARCH models show higher returns than MLE-based GARCH in the testing period. And trading profitable percentages of MLE-based GARCH IVTS models range from 47.5% to 50.0%, trading profitable percentages of SVR-based GARCH IVTS models range from 51.8% to 59.7%. MLE-based symmetric S-GARCH shows +150.2% return and SVR-based symmetric S-GARCH shows +526.4% return. MLE-based asymmetric E-GARCH shows -72% return and SVR-based asymmetric E-GARCH shows +245.6% return. MLE-based asymmetric GJR-GARCH shows -98.7% return and SVR-based asymmetric GJR-GARCH shows +126.3% return. Linear kernel function shows higher trading returns than radial kernel function. Best performance of SVR-based IVTS is +526.4% and that of MLE-based IVTS is +150.2%. SVR-based GARCH IVTS shows higher trading frequency. This study has some limitations. Our models are solely based on SVR. Other artificial intelligence models are needed to search for better performance. We do not consider costs incurred in the trading process including brokerage commissions and slippage costs. IVTS trading performance is unreal since we use historical volatility values as trading objects. The exact forecasting of stock market volatility is essential in the real trading as well as asset pricing models. Further studies on other machine learning-based GARCH models can give better information for the stock market investors.
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