• BMB Reports
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• 제36권4호
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• pp.421-425
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• 2003

The serine/threonine protein kinase family is a large and diverse group of enzymes that are involved in the regulation of multiple cellular pathways. Elevated kinase activity has been implicated in many diseases and frequently targeted for the development of pharmacological inhibitors. Therefore, non-radioactive antibody-based kinase assays that allow high throughput screening of compound libraries have been developed. However, they require a generation of antibodies against the phosphorylated form of a specific substrate. We report here a time-resolved fluorescence assay platform that utilizes a commercially-available generic anti-phosphothreonine antibody and permits assaying kinases that are able to phosporylate threonin residues on protein substrates. Using this approach, we developed an assay for Cdc7/Dbf4 kinase activity, determined the $K_m$ for ATP, and identified rottlerin as a non-ATP competitive inhibitor of this enzyme.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.140-140
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• 2013

This presentation will describe recent developement in spatially and time resolved optical diagnostics for two kinds of the high pressure microdischarges. The first kind is a nanosecond pulsed discharge with two pin electrodes while the second kind is a microwave split ring resonator developed by Jeff Hopwood. Both spatially and time resolved optical emissions are collected for these two discharges and some interesting phenomena are observed. By using either the Stark broadening or a collisional radiative model for high pressure discharges, the evolution of electron density can be obtained. We will compare these different techniques for obtaining the electron density and discuss their limitations.

• 한국광학회:학술대회논문집
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• 한국광학회 2002년도 하계학술발표회
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• pp.90-91
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• 2002

강한 펄스 레이저가 렌즈에 의해 투명한 매질 속에 집광되면 가시광선 영역에서부터 적외선 영역에 걸친 넓은 파장대의 빛이 발생하게 되는데 이것을 White-Light Continuum이라고 한다. 비선형 결정인 $\beta$-BBO에 의해서도 넓은 영역의 백색광을 발생시킬 수 있다. 이러한 펄스 백색광은 Time-resolved spectroscopy에서의 Probe beam과 다양한 파장대의 펄스 레이저 발생 및 증폭을 위한 Seed pulse 등으로 응용되고 있다. (중략)

• 대한의용생체공학회:의공학회지
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• 제18권4호
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• pp.499-505
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• 1997

본 연구에서는 생체조직의 흡수계수와 산란계수를 비침습적으로 측정하는 방법에 관하여 연구하였다. 피코초 영역의 펄스폭을 갖는 레이저광으로 조직을 조사하고 산란반사된 빛을 시간상관 단일 광자 계수법을 이용하여 피코초 시간영역에서 측정하였다. 측정된 시간분해 반사율의 최대치에 이르는 시간 및 나중소멸부분의 점근선의 기울기로부터 계산되는 값과 디컨블루션방법에 의한 곡선맞춤으로 얻은 값을 비교하여 서로 잘 일치함을 확인하였다. 매질의 산란이 커질수록 흡수가 작을수록 근사식은 더 잘 맞으므로 가시광선부터 근적외선의 파장영역에서 흡수에 비해 산란이 매우 큰 생체조직의 광특성을 비침습적으로 측정하는 중요한 방법이 될 수 있다.

• Bulletin of the Korean Chemical Society
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• 제20권6호
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• pp.657-662
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• 1999

The variation of Jahn-Teller(J-T) distortion imposed on various metallo- tetraphenylporphyrins(MTPPS;M=ZnⅡ,PdⅡ,PtⅡ,and RhⅢ) has been investigated by time-resolved resonance Raman spectroscopy. B1g and B2g modes of the triplet(π,π*) states for the heavy-metal porphyrins exhibit the enhancement of their intensities compared with those of 3ZnⅢTPP, while the enhancement of phenyl internal mode is reduced. These results suggest that the J-T distortion becomes manifest as the metal size increases, and consequently the porphyrin-to-phenyl ring charge transfer in the excited triplet state is inhibited.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.2101-2101
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• 2001

Near-infrared spectroscopy is now being used in clinical diagnosis as a non-invasive monitor of tissue oxygenation state. However, due to lack of the optical pathlength information within tissues, it is still difficult to quantitate the hemoglobin concentration with present CW techniques. Time-resolved spectroscopy (TRS), which measures temporal profiles of emerging light from tissues, enables to estimate the pathlength distribution within tissues by converting time to distance. Consequently, quantitative measurement of tissue oxygenation is possible by analyzing the data with optical diffusion equation 1) or our Microscopic Beer-Lambert law2). Time-Resolved Spectroscopy System : TRS-1O3) Our TRS-10 system consists of a three-wavelength (759, 797, 833 nm) PLP as pulsed light source, a high speed PMT with high sensitivity and three signal-processing circuits for time-resolved measurement (CFD/TAC, A/D converter and histogram memory). Optical pulse train consisting of 759, 797 and 833nm is generated by PLP at 5㎒ repetition rate and irradiated a sample through a single optical fiber. The diffuse-reflected light from the sample is collected by a bundle fiber and then detected by the PMT for single photon measurement. After being amplified by a following fast amplifier, the electrical signals for each wavelength are picked out by CFD/TAC module. Then, a signal processing circuit integrated the TRS data for each wavelength individually. The simultaneous TRS measurement for three wavelengths achieved without any optical or mechanical switch. Experiment and Results Input and detection fibers of TRS-10 were attached at the human forehead with a fiber separation of 3cm. TRS measurements were continuously performed for about 20 minutes including 2 minutes hyper ventilation. It was observed that the total hemoglobin concentration was decreasing during the hyper ventilation and recovered until 2 minutes after hyper ventilation. On the other hand, the deoxy-hemoglobin concentration began to increase after hyper ventilation and had its peak at around 2 minute later, showing 502 drop from 75% to 60% due to inhibition of breathing by performing hyper ventilation. The results showed that this system might be able to quantitate the concentrations of oxy- and deoxy-hemoglobin in the human brain.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2009년도 춘계 종합학술대회 논문집
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• pp.1105-1110
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• 2009

Time Resolved Imaging of Contrast Kinetics 기법을 이용한 4차원 자기공명혈관조영술(TRICKS -MRA)영상과 기존 TOF-MRA영상을 비교하여 임상적 유용성에 대해 알아보고자 하였다. 총 17명 중 뇌 혈관 질환이 의심되는 11명과 뇌 종양을 의심하는 6명을 대상으로 TOF-MRA와 TRICKS -MRA을 시행한 후 17대상자 중 11명에서 DSA를 추가적으로 시행하였다. TOF-MRA는 공간해상력보다 시간해상력이 부족하였고, TRICKS-MRA에서는 TOF-MRA보다 공간분해능은 부족한 점이 많지만 영상판독에 큰 영향은 없었고 모든 대상자에서 동맥과 정맥의 구별이 가능하여 시간분해능이 우수 한 것을 나타났다. 또한 종양과 혈관과의 관계성 평가에서 TOF-MRA보다 좋은 것으로 나타났다. TRICKS-MRA방법은 시간대별로 동맥, 모세혈관, 정맥기를 신속하면서 쉽게 4차원적으로 영상화가 가능하여 앞으로 임상에서 이용도가 더욱 증가 할 것으로 사료된다.

• Bulletin of the Korean Chemical Society
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• 제23권2호
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• pp.267-270
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• 2002

Photodissociations of o-, m-, and p-iodotoluene radical cations were investigated by using Fourier-transform ion cyclotron resonance (FT-ICR) spectrometry. Iodotoluene radical cations were prepared in an ICR cell by a photoionization charge-transfer method. The time-resolved one-photon dissociation spectra were obtained at 532 nm and the identities of $C_7H_7^+$ products were determined by examining their bimolecular reactivities toward toluene-$d_8$. The two-photon dissociation spectra were also recorded in the wavelength range 615-670 nm. The laser power dependence, the temporal variation, and the identities of $C_7H_7^+$ were examined at 640 nm. The mechanism of unimolecular dissociation of iodotoluene radical cations is elucidated: the lowest barrier rearrangement channel leads exclusively to the formation of the benzyl cation, whereas the direct C-I cleavage channel yields the tolyl cations that rearrange to both benzyl and tropylium cations with dissimilar branching ratios among o-, m-, and p-isomers. With a two-photon energy of 3.87 eV at 640 nm, the direct C-I cleavage channel results in the product branching ratio, [tropylium cation]/[benzyl cation], in descending order, 0.16 for meta >0.09 for ortho >0.05 for para.

• 한국레이저가공학회지
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• 제14권4호
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• pp.21-27
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• 2011

In this work, we have studied on time-resolved transient reflective image of single crystalline Si surface after single-shot fs-laser irradiation with varying the laser fluence under two different laser spot sizes. The temporal profiles of transient reflectivity changes as well as its maximum values at the early delay time were found to be strongly dependent on both the laser beam spot size and laser fluence. We have interpreted the dependence of transient reflectivity changes on the laser spot size in terms of a relaxation of the generated free carriers to the bulk silicon, which should be interacted with the plasma.

• 광학과기술
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• 제8권4호
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• pp.30-34
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• 2004