Measurement of Absorption and Scattering Coefficients of Biological Tissues by Time-Resolved Reflectance Method

시간 분해 반사율에 의한 생체조직의 흡수계수와 산란계수 측정

  • Published : 1997.12.01

Abstract

A non-invasive technique to measure absorption and scattering coefficients was investigated The reflected backscattered light from the surface of phantom and biological tissue was obtained by using a time-correlated single photon counting system in pico-second time domain. The absorption and scattering coefficients were acquired by the time of peak and asymptotic behavior of the time-resolved reflectance curve and agreed well the ones that is obtained with deconvolution method It was found that the approximation method was good for biological medium to calculate optical properties due to its convenience and accuracy.

본 연구에서는 생체조직의 흡수계수와 산란계수를 비침습적으로 측정하는 방법에 관하여 연구하였다. 피코초 영역의 펄스폭을 갖는 레이저광으로 조직을 조사하고 산란반사된 빛을 시간상관 단일 광자 계수법을 이용하여 피코초 시간영역에서 측정하였다. 측정된 시간분해 반사율의 최대치에 이르는 시간 및 나중소멸부분의 점근선의 기울기로부터 계산되는 값과 디컨블루션방법에 의한 곡선맞춤으로 얻은 값을 비교하여 서로 잘 일치함을 확인하였다. 매질의 산란이 커질수록 흡수가 작을수록 근사식은 더 잘 맞으므로 가시광선부터 근적외선의 파장영역에서 흡수에 비해 산란이 매우 큰 생체조직의 광특성을 비침습적으로 측정하는 중요한 방법이 될 수 있다.

Keywords

References

  1. IEEE Trans. Biomed. Eng. v.36 Time-resolved reflectance spectroscopy in turbid tissues S.L. Jacques
  2. IEEE J. Quantum Electron v.26 Optical reflectance and transmittance of tissues:principles and applications B.C.Wilson;S.L.Jacques
  3. Photochem. Photobiol v.42 In vivo and postmortem measurements of the attenuation spectra of light in mammalian tissues B.C. Wilson;W.P. Jeeves;D.M. Lowe
  4. Photochem. Photobiol v.43S Environmental effects on light transport in tissues in vitro(400-1100 nm) L.E. Preuss;P.F.Bolin;R.C.Talyor
  5. Med. Phys. v.14 Total attenuation coefficients and scattering phase functions of tissues and phantom materials at 633nm S.T.Flock;B.C.Wilson;M.S.Patterson
  6. Appl. Opt. v.28 Extinction and absorption coefficients and scattering phase functions of human tissues in vitro R.Marchesini;A.Bertoni;S.Andreola;E.Melloni;A.E.Sichirollo
  7. Multiple Light Scattering Tables, Formulas and Application H.C.van de Hult
  8. Appl. Opt. v.28 Time resolved refletance and transmittance for the non-invasive measurement of tissue opticak properties M.S.Patterson;B.Chance;B.C.Wilson
  9. IEEE J. Quantum Electon v.30 Time-resolved reflectance; a systmatic study for application to the optical characterization fo tissues R. Cubeddu;M. Musolino;A.D. Heri;P. Tarano;G. Valentini
  10. Phys. Rev. Lett. v.64 When does the diffusion approximation fail to describe photon transport in random media? K.M.Yoo;F.Liu;R.R.Alfano
  11. Wave propagation and scattering in random media v.1 A.Ishimaru
  12. Radiative Transfer S. Chandrasekhar
  13. Med. Phys. v.10 A Monte Carlo model for the absorption and flux distribution of light in tissues B.C.Wilson;G.Adam
  14. Appl. Opt. v.32 Coherent backcattering in biological media:measurement and estimation of optical properties G.Yoon;D.N.Ghosh Roy;R.C.Straight
  15. Phtochem. Photobiol. v.46 In derect versus direct techniques for the measurement of the optical properties tissues B.C.Wilson;M.S.Patterson;S.T.Flock