• Journal of the Korean Society of Food Culture
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• v.33 no.5
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• pp.464-471
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• 2018

Purpose: This study examined the effects of ${\alpha}$-lipoic acid in diluted solvents on cell growth in 3T3-L1 cells according to the treated concentration and times. Methods: Adipocyte 3T3-L1 cell were cultured. Confluent cells underwent starvation with SFM for 1 day and then were cultured in a medium containing various concentrations 0, 100, 200, and $400{\mu}mol/L$ of ${\alpha}$-lipoic acid. The cell viability was measured using the EZ Cytox assay kit. In addition, the effect of ${\alpha}$-lipoic acid of diluted solvents on the cell growth in 3T3-L1cells was examined according to the treated concentration and times. Results: The ${\alpha}$-lipoic acid diluted ethanol inhibited cell proliferation in a dose and time dependent manner. The ${\alpha}$-lipoic acid diluted ethanol induced adipocyte 3T3-L1 cells proliferation with an adipocyte inducer. In addition, ${\alpha}$-lipoic acid inhibited adipocyte 3T3-L1 growth in a dose and time dependent manner (p<0.05). Conclusion: This study showed that a treatment with ${\alpha}$-lipoic acid diluted ethanol inhibits cell growth of, adipocyte 3T3-L1 cells induced with an adipocyte inducer, ($200{\mu}mol/L$ of ${\alpha}$-lipoic acid) treated for 48 hr.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.1A
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• pp.15-24
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• 2008

This paper presents the analytical method for the long-term behavior of simply supported composite beams with precast decks prestressed in the longitudinal direction. The objectives of time-dependent analysis are to estimate losses of prestress on the concrete slab and long-term deflection due to creep and shrinkage of concrete, relaxation of prestressing steel. Also, the time-dependent analysis was carried out using the presented analytical method to evaluate the effects of several parameters on the long-term behavior of composite bridge with precast deck, including geometrical shapes of composite beams, compressive strength of concrete and magnitude of initial prestress. The results of the analysis indicated that, in the effects of geometrical shapes of composite beams, the main parameters affecting the losses of prestress and the long-term deflection were the cross sectional area and the moment of inertia of steel beam, respectively. Finally, the determination method for the required initial prestress was proposed by evaluation of the loss characteristics due to shrinkage and creep of concrete.

• KSBB Journal
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• v.27 no.5
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• pp.289-294
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• 2012

Resveratrol, natural polyphenol in grapes and red wine, is known to have the anti-proliferatory and anti-angiogenic effects in various cancer cells. In this study, we have investigated the effects of resveratrol in HT-29 colon cancer cells. Treatment of resveratrol in different concentrations and time inhibited proliferation of HT-29 colon cancer cells. We explored the effects of resveratrol on HT-29 colon cancer cell motility using a wound healing assay. In the absence of the resveratrol, the HT-29 cells are migrated along the edges of the wound and showed a large-scale migration, whereas dose- and time-dependent inhibition of cell flattening and spreading was observed in the presence of resveratrol. Resveratrol inhibited MMP-9 in a dose- and time-dependent on HT-29 colon cancer cells by Western blotting. In addition, resveratrol increased AMPK activity and decreased COX-2, VASP and VEGF expression. Treatment of compound C inhibited AMPK activity, however, the expression of VASP and COX-2 increased thus, COX-2 and VASP are modulated by AMPK. However treatment of celecoxib could not control AMPK activity but decreased VEGF expression. We suggest that resveratrol inhibits cell proliferation and migration through activation of AMPK and decreased COX-2, VASP and VEGF expression in HT-29 colon cancer cells.

• Journal of Nutrition and Health
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• v.54 no.4
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• pp.335-341
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• 2021

Purpose: Muscle mitochondria play a key role in regulating fatty acid and glucose metabolism. Dysfunction of muscle mitochondria is associated with metabolic diseases such as obesity and type 2 diabetes. Isorhamnetin (ISOR), also known as 3-O-methylquercetin, a quercetin metabolite, is a naturally occurring flavonoid in many plants. This study evaluated the effects of ISOR on the regulation of the mitochondrial function of C2C12 muscle cells. Methods: C2C12 muscle cells were differentiated for 5 days, and then treated in various concentrations of ISOR. Cytotoxicity was determined by assessing cell viability using the water-soluble tetrazolium salt-8 assay principle at different concentrations of ISOR and time points. Levels of the mitochondrial DNA (mtDNA) content and gene expression were measured by quantitative real-time polymerase chain reaction. The citrate synthase (CS) activity was quantified by the enzymatic method. Results: ISOR at a concentration of 10 µM did not show any cytotoxic effects. ISOR increased the mtDNA copy number in a time- or dose-dependent manner. The messenger RNA levels of genes involved in mitochondrial function, such as peroxisome proliferator-activated receptor-γ coactivator-1α, and uncoupling protein 3 were significantly stimulated by the ISOR treatment. The CS activity was also significantly increased in a time- or dose-dependent manner. Conclusion: These results suggest that ISOR enhances the regulation of mitochondrial function, which was at least partially mediated via the stimulation of the mtDNA replication, mitochondrial gene expression, and CS activity in C2C12 muscle cells. Therefore, ISOR may be useful as a potential food ingredient to prevent metabolic diseases-associated muscle mitochondrial dysfunction.

• Journal of Korean society of Dental Hygiene
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• v.10 no.3
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• pp.531-540
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• 2010

Objectives : In this study, following the cavity restorations with low copper conventional alloy, high copper admixed one and high copper unicompositional one, which are used the most frequently in a clinical setting at the present, to experiment the time-dependent changes of strength, bubbles were examined. Besides, to examine the detrimental effects of mercury contained in dental amalgam, the amount of mercury release was evaluated. Methods : As dental amalgams which were used herein, [BESTALOY], [Hi-Aristaloy 21] and [Sybraloy] were selected for a low-copper conventional amalgam, a high-copper admixed one and a high-copper unicompositional one in the corresponding order. The formation of bubbles and the weight ratio of mercury release were evaluated using a field emission scanning electron microscope (FE-SEM). Thus, the following results were obtained: Results : 1. The time-dependent amount of mercury release reached a statistical significance in three types of alloys, which was shown in such a descending order as [BESTALOY], [Hi-Aristaloy 21] and [Sybraloy]. 2. A low-copper conventional type, BESTALOY is a cutting type and it was found to have an increased formation of fine bubbles. In the remaining two types, [Hi-Aristaloy 21] (a high-copper admixed alloy) and [Sybraloy] (a high-copper unicompositional alloy), the time-dependent changes in the formation of bubbles was negligible. Conclusions : Accordingly, this type of mercury release from amalgam alloy denotes the difference in the weight ratio of total constituents between after 24 hours and after two weeks. But further studies are warranted to examine the amount of mercury release which is detrimental to human bodies. Besides, a low-copper conventional alloy is a cutting type and it was characterized by the abundant formation of bubbles in a time-dependent manner. This implies that the strength of amalgam is impaired, which should be considered in selecting the appropriate amalgam alloy in a clinical setting.

• Steel and Composite Structures
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• v.17 no.4
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• pp.405-429
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• 2014

In this paper, it is aimed to determine the structural behavior of suspension bridges considering construction stages and different soil conditions. Bosporus Suspension Bridge connecting the Europe and Asia in Istanbul is selected as an example. Finite element model of the bridge is constituted using SAP2000 program considering existing drawings. Geometric nonlinearities are taken into consideration in the analysis using P-Delta large displacement criterion. The time dependent material strength of steel and concrete and geometric variations is included in the analysis. Time dependent material properties are considered as compressive strength, aging, shrinkage and creep for concrete, and relaxation for steel. To emphases the soil condition effect on the structural behavior of suspension bridges, each of hard, medium and soft soils are considered in the analysis. The structural behavior of the bridge at different construction stages and different soil conditions has been examined. Two different finite element analyses with and without construction stages are carried out and results are compared with each other. At the end of the analyses, variation of the displacement and internal forces such as bending moment, axial forces and shear forces for bridge deck and towers are given in detail. Also, displacement and stresses for bridge foundation are given with detail. It can be seen from the analyses that there are some differences between both analyses (with and without construction stages) and the results obtained from the construction stages are bigger. It can be stated that the analysis without construction stages cannot give the reliable solutions. In addition, soil condition have effect on the structural behavior of the bridge. So, it is thought that construction stage analysis using time dependent material properties, geometric nonlinearity and soil conditions effects should be considered in order to obtain more realistic structural behavior of suspension bridges.

• Journal of the Korea Concrete Institute
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• v.18 no.6 s.96
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• pp.711-720
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• 2006

The structural behavior of concrete girder in bridges constructed by free cantilever method is time-dependent due to creep and shrinkage of concrete. The constraint effects of longitudinal movement of concrete girders can introduce unfavourable moment into piers. This study is aimed at proposing a method to reduce the moment of piers in bridge constructed by free cantilever method. The method are systematically composed of time-dependent structural analysis of bridges and loading of control force during construction of bridge. Numerical analyses are carried out depending on the parameters such as amount of control force and flexibility of pier. Time-dependent structural behavior shows that moment of pier increases according as pier height decreases. Also, moment of pier decreases when control method are applied. Numerical result of the study represents that time-dependent moment of piers can be controlled effectively by employing the proposed method.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2000.06a
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• pp.114-120
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• 2000

EHL analysis of connecting rod bearing is proposed which includes effects of temperature variation in lubrication film. Lubrication film temperature is treated as a time-dependent, two-dimensional variable which is averaged over the film thickness, while connecting rod big end temperature is assumed to be time-independent and three-dimensional. It is assumed that a portion of the heat generated by viscous dissipation in the lubrication Him is absorbed by the film itself, and the remainder flows into the bearing surface. Mass-conserving cavitation algorithm is applied and the effect of variable viscosity is included to solve the Reynolds equation. Simulation results of the connecting rod bearing of internal combustion engine are presented. It is shown that the temperature variation has remarkable effects on the bearing performance. It is concluded that the EHL analysis considering effects of the temperature variation is strongly recommended to predict the bearing performance of connecting rod big end On internal combustion engine.

• Tribology and Lubricants
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• v.17 no.3
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• pp.228-235
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• 2001

EHL analysis of connecting rod bearing is proposed which includes effects of temperature variation in lubrication film. Lubrication film temperature is treated as a time-dependent, two-dimensional variable which is averaged over the film thickness, while connecting rod big end temperature is assumed to be time-independent and three-dimensional. It is assumed that a portion of the heat generated by viscous dissipation in the lubrication film is absorbed by the film itself, and the remainder flows into the bearing surface. Mass-conserving cavitation algorithm is applied and the effect of variable viscosity is included to solve the Reynolds equation. Simulation results of the connecting rod bearing in internal combustion engine are presented. It is shown that the temperature variation has remarkable effects on the bearing performance. It is concluded that the EHL analysis considering effects of the temperature variation is strongly recommended to predict the connecting rod bearing performance in internal combustion engine.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.13 no.21
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• pp.91-100
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• 1990

This paper is concerned with the development of optimal algorithms for multi-stage flowshop scheduling problems with sequence dependent setup times. In the previous researches the setup time of a job is considered to be able to begin at the earliest opportunity given a particular sequence at the start of operations. In this paper the setup time of a job is considered to be able to begin only at the completion of that job on the previous machine to reflect the effects of the setup time to the performance measure of sequence dependent setup time flowshop scheduling. The results of the study consist of two areas; first, a general integer programming(IP) model is formulated and a nixed integer linear programming(MILP) model is also formulated by introducing a new binary variable. Second a depth-first branch and bound algorithm is developed. To reduce the computational burdens we use the best heuristic schedule developed by Choi(1989) as the first trial. The experiments for developed algorithm are designed for a 4$\times$3$\times$3 factorial design with 360 observations. The experimental factors are PS(ratio of processing time to setup time), M(number of machines), N(number of jobs).