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http://dx.doi.org/10.7841/ksbbj.2012.27.5.289

Effects of Resveratrol on Migration and Proliferation in HT-29 Colon Cancer Cells  

Lee, Sol Hwa (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
Park, Song Yi (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
Kim, In-Seop (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
Park, Ock Jin (Department of Food and Nutrition, College of Life Science and Nano Technology, Hannam University)
Kim, Young Min (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
Publication Information
KSBB Journal / v.27, no.5, 2012 , pp. 289-294 More about this Journal
Abstract
Resveratrol, natural polyphenol in grapes and red wine, is known to have the anti-proliferatory and anti-angiogenic effects in various cancer cells. In this study, we have investigated the effects of resveratrol in HT-29 colon cancer cells. Treatment of resveratrol in different concentrations and time inhibited proliferation of HT-29 colon cancer cells. We explored the effects of resveratrol on HT-29 colon cancer cell motility using a wound healing assay. In the absence of the resveratrol, the HT-29 cells are migrated along the edges of the wound and showed a large-scale migration, whereas dose- and time-dependent inhibition of cell flattening and spreading was observed in the presence of resveratrol. Resveratrol inhibited MMP-9 in a dose- and time-dependent on HT-29 colon cancer cells by Western blotting. In addition, resveratrol increased AMPK activity and decreased COX-2, VASP and VEGF expression. Treatment of compound C inhibited AMPK activity, however, the expression of VASP and COX-2 increased thus, COX-2 and VASP are modulated by AMPK. However treatment of celecoxib could not control AMPK activity but decreased VEGF expression. We suggest that resveratrol inhibits cell proliferation and migration through activation of AMPK and decreased COX-2, VASP and VEGF expression in HT-29 colon cancer cells.
Keywords
Resveratrol; COX-2; VASP; VEGF; angiogenesis;
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