• YAKHAK HOEJI
• /
• v.48 no.3
• /
• pp.189-196
• /
• 2004

Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) playa key role in tumor invasion and metastasis. As an inhibitor of MMP-2, TIMP-2 is known to block both the invasive and metastatic behavior of cancer cells, and decrease tumor growth activity. We performed this study to investigate the effects of TIMP-2 over-expression induced by retroviral mediated gene transfer in vitro and in vivo. The human colon cancer cell line SW480 was transfected with the retroviral vector encoding TIMP-2. The effects of TIMP-2 over-expression were analyzed by invasion assay and gelatinase activity test in colon cancer cells and tumorigencity in nude mice. In evaluation of the transfection efficiency of the retroviral vector encoding TIMP-2 in colon cancer cells, we confirmed up-regulation of TIMP-2 expression dependent on the time of cell culture. In addition, inhibition of MMP-2 expression in SW480/TIMP-2 was shown by gelatin zymography. In the in vitro invasion assay SW480/TIMP-2 inhibited the invasiveness on matrigel coated with collagen. To determine whether TIMP-2 can modulate in vivo tumorigenicity and metastasis, SW480/TIMP-2 cells were injected subcutaneously in nude mice. The tumor mass formation of SW480/TIMP-2 cells in nude mice was markedly decreased compared to nontransfected cancer cells. These results showed that colon cancer cells transfected with the retroviral vector encoding TIMP-2 inhibits the invasiveness in vitro and tumorigenicity in vivo.

• Structural Engineering and Mechanics
• /
• v.60 no.6
• /
• pp.1021-1038
• /
• 2016

Considerable part of reinforced concrete building has suffered from destructive earthquakes in Turkey. This situation makes necessary to determine nonlinear behavior and seismic performance of existing RC buildings. Inelastic response of buildings to static and dynamic actions should be determined by considering both flexural plastic hinges and brittle shear hinges. However, shear capacities of members are generally neglected due to time saving issues and convergence problems and only flexural response of buildings are considered in performance assessment studies. On the other hand, recent earthquakes showed that the performance of older buildings is mostly controlled by shear capacities of members rather than flexure. Demand estimation is as important as capacity estimation for the reliable performance prediction in existing RC buildings. Demand estimation methods based on strength reduction factor (R), ductility (${\mu}$), and period (T) parameters ($R-{\mu}-T$) and damping dependent demand formulations are widely discussed and studied by various researchers. Adopted form of $R-{\mu}-T$ based demand estimation method presented in Eurocode 8 and Turkish Earthquake Code-2007 and damping based Capacity Spectrum Method presented in ATC-40 document are the typical examples of these two different approaches. In this study, eight different existing RC buildings, constructed before and after Turkish Earthquake Code-1998, are selected. Capacity curves of selected buildings are obtained with and without considering the brittle shear capacities of members. Seismic drift demands occurred in buildings are determined by using both $R-{\mu}-T$ and damping based estimation methods. Results have shown that not only capacity estimation methods but also demand estimation approaches affect the performance of buildings notably. It is concluded that including or excluding the shear capacity of members in nonlinear modeling of existing buildings significantly affects the strength and deformation capacities and hence the performance of buildings.

• Safety and Health at Work
• /
• v.12 no.3
• /
• pp.324-338
• /
• 2021

Background: We hypothesized that the growing demand of Korean workers for work-life balance would change the factors influencing job satisfaction. We sought to verify our hypothesis by conducting a conjoint analysis based on the Korean Working Conditions Survey (KWCS). Methods: We analyzed the raw data of the KWCS, conducted by the Occupational Safety and Health Research Institute from 2006 to 2017. To complete the analysis, we counted on a conjoint model of analysis, typically used in the analysis of customer satisfaction. The dependent variable was the satisfaction of workers with their working conditions, and the independent variables were the job quality indicators identified by Eurofound. Results: The factors that have the greatest impact on working conditions satisfaction are summarized as follows: "physical environment" for the first wave, "adverse social behavior" for the second wave, "occupational status" for the third and fourth waves, and "management quality" for the fifth wave. "Earnings" were not a major factor in determining employee job satisfaction, and the relative importance index is decreasing. Conclusion: According to the results of the analysis of the tendencies of Korean workers, the factors that affect the satisfaction with the working conditions have changed over time. It is crucial to identify factors that affect working conditions to assure the health and productivity of workers. The results of this study demonstrate that policymakers and employers are required to attentively consider human relations and social environment at work to improve working conditions in the future.

• Advances in nano research
• /
• v.7 no.4
• /
• pp.249-263
• /
• 2019

In the current paper, an exact solution method is carried out for analyzing the thermo-mechanical vibration of curved FG nano-beams subjected to uniform thermal environmental conditions, by considering porosity distribution via nonlocal strain gradient beam theory for the first time. Nonlocal strain gradient elasticity theory is adopted to consider the size effects in which the stress for not only the nonlocal stress field but also the strain gradients stress field is considered. It is perceived that during manufacturing of functionally graded materials (FGMs) porosities and micro-voids can be occurred inside the material. Material properties of curved porous FG nanobeam are assumed to be temperature-dependent and are supposed to vary through the thickness direction of beam which modeled via modified power-law rule. Since variation of pores along the thickness direction influences the mechanical and physical properties, porosity play a key role in the mechanical response of curved FG nano-structures. The governing equations and related boundary condition of curved porous FG nanobeam under temperature field are derived via the energy method based on Timoshenko beam theory. An analytical Navier solution procedure is utilized to achieve the natural frequencies of porous FG curved nanobeam supposed to thermal loading. The results for simpler states are confirmed with known data in the literature. The effects of various parameters such as nonlocality parameter, porosity volume fractions, thermal effect, gradient index, opening angle and aspect ratio on the natural frequency of curved FG porous nanobeam are successfully discussed. It is concluded that these parameters play key roles on the dynamic behavior of porous FG curved nanobeam. Presented numerical results can serve as benchmarks for future analyses of curve FG nanobeam with porosity phases.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.35 no.5
• /
• pp.516-521
• /
• 2022

We investigated the microstructure, crystal structure, dielectric, and elecromechanical strain properties of lead-free BaTiO₃ (BT)-modified (Bi_1/2Na_1/2)TiO₃-SrTiO₃ (BNT-ST) piezoelectric ceramics. Samples were prepared by a conventional ceramic processing route. Temperature dependent dielectric properties confirmed that a phase transition from a nonergodic relaxor to an ergodic relaxor was induced when the BT concentration reached 1.5 mol%, interestingly, where the average grain size reached a maximum value of 4.5 ㎛. At the same time, enhanced electromechanical strain (S_max/E_max = 600 pm/V) was obtained. It is suggested that the induced ferroelectric-relaxor phase transition by the BT modification is responsible for the enhancement of electromechanical strain in 1.5 mol% BT-modified BNT-ST ceramics.

• Journal of Powder Materials
• /
• v.30 no.2
• /
• pp.146-155
• /
• 2023

The Ti-6Al-4V lattice structure is widely used in the aerospace industry owing to its high specific strength, specific stiffness, and energy absorption. The quality, performance, and surface roughness of the additively manufactured parts are significantly dependent on various process parameters. Therefore, it is important to study process parameter optimization for relative density and surface roughness control. Here, the part density and surface roughness are examined according to the hatching space, laser power, and scan rotation during laser-powder bed fusion (LPBF), and the optimal process parameters for LPBF are investigated. It has high density and low surface roughness in the specific process parameter ranges of hatching space (0.06-0.12 mm), laser power (225-325 W), and scan rotation (15°). In addition, to investigate the compressive behavior of the lattice structure, a finite element analysis is performed based on the homogenization method. Finite element analysis using the homogenization method indicates that the number of elements decreases from 437,710 to 27 and the analysis time decreases from 3,360 to 9 s. In addition, to verify the reliability of this method, stress-strain data from the compression test and analysis are compared.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.28 no.5A
• /
• pp.747-756
• /
• 2008

The purpose of this paper is to investigate the effect of the important parameters on the transfer length during the dynamic flame cutting of tendon experimentally. The considered parameters were strand diameter, concrete cover thickness, stirrup, debonding strand and release method. Ten pretensioned concete beam specimens were cast and tested. Time history curves for the axial strain of tendon were measured by electrical resistance strain gauges mounted on the strands. Experimental results indicated that large dynamic shock effects occurred near cut-end during the sudden release. The prestressing forces are dependent on the parameters above considered. The ratio of residual prestressing forces of 12.7 mm strands is greater than 15.2 mm strands. Using debonding strand and gradual release are more efficient for applying prestressing forces.

• Safety and Health at Work
• /
• v.14 no.3
• /
• pp.279-286
• /
• 2023

Background: This study aimed to evaluate the association between exposure to occupational hazards and the metabolic syndrome. A secondary objective was to analyze the additive and multiplicative effects of exposure to risk factors. Methods: This retrospective cohort was based on 31,615 health examinees at the Pusan National University Yangsan Hospital in Republic of Korea from 2012-2021. Demographic and behavior-related risk factors were treated as confounding factors, whereas three physical factors, 19 organic solvents and aerosols, and 13 metals and dust were considered occupational risk factors. Time-dependent Cox regression analysis was used to calculate hazard ratios. Results: The risk of metabolic syndrome was significantly higher in night shift workers (hazard ratio = 1.45: 95% confidence interval = 1.36-1.54) and workers who were exposed to noise (1.15:1.07-1.24). Exposure to some other risk factors was also significantly associated with a higher risk of metabolic syndrome. They were dimethylformamide, acetonitrile, trichloroethylene, xylene, styrene, toluene, dichloromethane, copper, antimony, lead, copper, iron, welding fume, and manganese. Among the 28 significant pairs, 19 exhibited both positive additive and multiplicative effects. Conclusions: Exposure to single or combined occupational risk factors may increase the risk of developing metabolic syndrome. Working conditions should be monitored and improved to reduce exposure to occupational hazards and prevent the development of the metabolic syndrome.

• Journal of the Korean Geotechnical Society
• /
• v.40 no.3
• /
• pp.19-31
• /
• 2024

In Korea, driven piles are generally penetrated up to weathered rock or harder strata. Friction piles have been used to some extent in the southwest coastal area with deep soils; however, friction piles are not extensively due to uncertainties about construction quality. The embedded pile construction method is primarily used due to noise and vibration complaints. However, in Southeast Asian countries (e.g., Cambodia, Myanmar, and Vietnam), where soft sediments are deep, the driven pile method is commonly used due to its economic advantages. Construction companies are increasingly entering overseas construction markets, e.g., Southeast Asia; thus, it is necessary to understand the behavior of driven friction piles in the soil and improve on-site engineering management to gain market competitiveness in these countries. In this study, the bearing capacity of friction piles driven into clayey coastal soils in Vietnam with time-dependent characteristics was evaluated based on the dynamic and static pile load tests. Based on the results, a modified Danish formula is proposed for on-site quality management.

• Journal of the Korean Wood Science and Technology
• /
• v.52 no.4
• /
• pp.375-382
• /
• 2024

The study focuses on evaluating the bending creep performance of laminated veneer lumber (LVL) formwork in timber concrete composite (TCC) structures. Timber-framed construction is highlighted for its environmental benefits and seismic resistance, but limitations such as poor tensile strength and brittle failure in bending hinder its use in high-rise buildings. Wood-concrete hybrid structures, particularly those using reinforced concrete slabs with TCC floors, emerge as a potential solution. The research aims to understand the time-dependent behavior of TCC components, considering factors like wood and concrete shrinkage and connection creep. The experiment was conducted in western Japan on the TCC floor designed for use in the Kama-city Inatsuki-higashi compulsory education school. The LVL formwork, measuring 9,000 mm by 900 mm, and concrete is loaded onto it for testing. The creep test periods are examined using concrete loading. It employs a comprehensive creep analysis, adhering to Japanese standards, involving deflection measurements and regression analysis to estimate the creep coefficient. Results indicate substantial deformation after shoring removal, suggesting potential reinforcement needs. The study recommends extending test periods for improved accuracy and recognizing regional climate impacts. Overall, the research provides valuable insights into the potential of LVL formwork in TCC structures, emphasizing safety considerations and paving the way for further experimentation under varied conditions to validate structural integrity.