In order to investigate radiation effects on the liver, functional changes of liver were analyzed after irradiation. Doses of 10 Gy, 15 Gy and 20 Gy were exposed partially to the liver of male rats(Sprague-Dawley) with X-ray(4MV linear accelerator) at room temperature. On 1, 2, 4 and 8 weeks after irradiation, sera of the animals were compared with those of unirradiated animal by liver function tests. Enzyme activities in sera such as alkaline phosphatase and concentrations of bilirubin in liver function tests. The content of the activities of many enzymes including alkaline phosphatase in sera were increased slightly with increasing exposure dose in all experiments and the activities of these enzymes increased markedly in 20 Gy irradiated groups. The contents of serum bilirubins including direct and indirect bilirubins increased continuously along with the time lapse after irradiation. However, in 20 Gy irradiated group, the content of serum bilirubin decreased slightly during 2 or 4 weeks after irradiation and increased markedly there after. From these above results, functional changes of the liver were induced in all irradiated groups. Damaged liver was recovered along with time collapsed after irradiation to the doses of 10 Gy and 15 Gy while no recovery was deteced within 8 weeks after irradiation to 20 Gy. These results suggest that careful attontion must be paid to liver not to be included in exposure field in radiation therapy.
An experiment was carried out to investigate the optimum doses of gamma-irradiation for sprouting inhibition of onion bulbs with irradiation time and storage conditions. The results. obtained are as follows: 1) The irradiation doses of 5, 7 and 10 krad, respectively, at 11, 32 and 66 days after harvest were sufficient to inhibit subsequent sprout of onion bulbs obtained from Nampyeong district. When they were irradiated at 96 day after harvest, however, there was little sprout-inhibition by 15 krad. In case of onion bulbs obtained from Changnyeong district, sprout was inhibited by doses of 8 and 12 krad respectively, at 51 and 89 days after harvest. 2) Low-temperature storage after irradiation was not effective in sprout-inhibition of onion bulbs. Onion bulbs stored at low temperature of $5^{\circ}C$ rather showed higher sprouting rate as compared with that of room temperature. 3) Rot increased in irradiated lot and at room temperature, and spores of Aspergillus sp. were little germinated at a level of 100 krad. 4) The respiratory rate of irradiated onion bulbs was higher immediately after irradiation but lower one week after irradiation than control. Respiratory quotient of tissues seems to be little affected by gamma-irradiation.
Purpose: To investigate the histopathological changes in the temporomandibular joint in streptozotocin-induced diabetic rat following irradiation. Materials and Methods : Sprague-Dawley rats weighing about 250 gm were divided into three groups: control, diabetic, and diabetic-irradiated groups. Diabetes mellitus was induced in the rats by injecting streptozotocin. Rats in the control group were injected with citrate buffer only. After 5 days, the head and neck region of the rats in diabetic-irradiated group were irradiated with single absorbed dose of 10 Gy. The rats were killed at 1, 3, 7, 14, 21, and 28 days after irradiation. The specimen including the temporomandibular joint were sectioned and observed using a histopathological method. Results : In the diabetic group, severe bone resorption in the mandibular condyle was observed throughout the period of experiment. Necrosis of bone marrow and trabeculae was observed at 28 days after diabetic state. Atrophy and fibrosis in the retrodisca] tissue was gradually progressed during the time of the experiment. In the diabetic-irradiated group, severe bone resorption in the mandibular condyle was observed during the early experimental phases, but regeneration of bone marrow was initiated at ]4 days after diabetic state and irradiation. A]so, calcification of abnormal trabeculae was observed at 28 days after diabetic state and irradiation. The retrodisca] tissue was degenerated in the early experimental phases, but it had been gradually regenerated during the experimental time. Conclusion: This experiment suggests that bone resorption and degeneration in the mandibular condyle are caused by the induction of diabetes, and abnormal bone formation is induced after irradiation in diabetic state.
Journal of Korean Academy of Oral and Maxillofacial Radiology
/
v.20
no.1
/
pp.53-62
/
1990
This experiment was performed to clarify the effects of /sup 60/Co gamma irradiation on secretory function, amylase activity and contents of nucleic acids of parotid gland in rat. Experimental animals were divided into 6th hours, 3rd, 7th, 14th and 28th days after irradiation and control. The experimental animals are singly irradiated with 20Gy (2,000rad) through protective lead block. Secretory function of parotid gland was evaluted by uptake and clearance of /sup 99m/TcO₄. /sup 99m/TcO₄. 0.2μ ci/gm, was injected into peritonium in uptake groups. Rats were sacrified with cervical dislocation after 30 minutes and gland was excised. In the clearance group. pilocarpine nitrate (8㎎/㎏) was intraperitoneally injected at 30 minutes after /sup 99m/TcO₄ injection and rats were sacrified 30 minutes after pilocarpine injection. Radioactivity of excised parotid gland was measured by using of gamma counter and stimulation-secretion coefficients, uptake radioactivity divided by clearance radioactivity, was calculated. Amylase activity and contents of DNA and RNA were determined by spectrophotometry. The results obtained were as follows: 1. In the uptake test, the radioactivity of /sup 99m/TcO₄ per unit weight increase in experimental group except 6th hours group, compared with control groups and showed a peak at 3rd days after irradiation. 2. In the clearance test, the radioactivity of /sup 99m/cO₄per unit weight rose to a peak at 3rd days after irradiation and gradually recovered thereafter. 3. Stimulation-secretion coefficient of parotid gland decreased at 6th hours, 3rd and 7th days after irradiation, and gradually increased. 4. Amylase activity of parotid gland decreased in 3rd and 7th days group, and especially lowest in 3rd days after irradiation. 5. The contents of DNA showed no definite difference in all the experimental groups, but RNA was seemed to decrease with time after irradiation.
Kim, Jae-Do;Chung, So-Hak;Hong, Young-Gi;Choi, Jang-Seok
The Journal of the Korean bone and joint tumor society
/
v.5
no.1
/
pp.1-8
/
1999
A single fraction of 50 Gy extracorporeal irradiation, as a modality of limb-sparing operation, has been used to achieve tumor necrosis in osteosarcoma. Although this modality of radiation therapy preserving the mobility of a joint is commonly practiced, the precise knowledge on the radiobiological response of osteosarcoma cell has remained to be elucidated. We therefore observed whether a single high dose irradiation caused apoptosis in osteosarcoma cells and whether the commitment to apoptosis was associated with cell kinetics. We also investigated radiation dose response along the time course for development of apoptosis following single high dose irradiation. The morphologic change in apoptosis was observed by fluorescence with Hoechst 33258 and the degree and the fraction of cells by flow cytometry. Irradiation of osteosarcoma cells with 10, 30 and 50 Gy resulted in chromatin condensation and apoptotic body formation. The degree of apoptosis in osteosarcoma cells was $29.5{\pm}3.56%$, $39.9{\pm}4.83%$ at 24 and 48 hours after 10 Gy irradiation ; $41.1{\pm}3.93%$, $66.9{\pm}5.21%$ at 24 and 48 hours after 30 Gy irradiation ; and $48.0{\pm}3.69%$, $75.6{\pm}4.65%$ at 24 and 48 hours after 50 Gy irradiation. The fraction of cells in cell-cycle kinetic was $39.2{\pm}4.3%$ in G2/M, $22.1{\pm}4.65%$ in G1 at 24 hours after 10 Gy irradiation ; $51.0{\pm}4.3%$ in G2/M, $20.4{\pm}4.7%$ in G1 at 48 hours after 10 Gy irradiation ; $40.3{\pm}3.9%$ in G2/M, $26.1{\pm}4.7%$ in G1 at 24 hours after 30 Gy irradiation ; $59.2{\pm}3.9%$ in G2/M, $5.9{\pm}5.1%$ in G1 at 48 hours after 30 Gy irradiation ; and $44.3{\pm}4.2%$ in G2/M, $21.1{\pm}3.5%$ in G1 at 24 hours after 50 Gy irradiation. The fraction of cells at 48 hours after 50 Gy irradiation could not be observed because of irradiation induced cell death of most of cells. All values for irradiated cells showed accumulation in G2/M phase and reduction in G1 phase, irrespective of irradiation dose. The results suggest that a single fraction of high dose irradiation with 50 Gy results in accumulation of cells at G2/M phase, leading to apoptosis.
Objective: We investigated the morphologic changes within 24 hours after a single ${\gamma}$-irradiation in the rat brain. Methods: Forty Sprague-Dawley rats were used. After a burr hole trephination on right parietal area, cerebral hemisphere was irradiated with 2Gy and 5Gy using iridium-192($^{192}Ir$), respectively. The effect was assessed at 4, 8, 12 and 24 hours after irradiation. The histological changes were scored following the detection of edema or disarray severity. TUNEL-positive cells exhibiting apoptotic morphology were counted in irradiated region. Results: Cortical edema and disarray were initially showed at 4 or 8 hour and almost all defined at 24 hour after irradiation. And the injury was wedge shape. TUNEL-positive cells were minimal at 8 hour after irradiation as the number of positive cells were $2.6{\pm}5.27$(n=5) after 2Gy, and $0.8{\pm}0.84$(n=5) after 5Gy. But, the number of apoptotic cells were increased markedly to $60{\pm}6.24$ at 12 hour after 2Gy and to $104{\pm}19.7$ at 24 hour after 5Gy. Conclusion: There were prominent morphologic changes immediately after ${\gamma}$-irradiation. And, apoptosis was increased according to the time period. These findings implicate that brain irradiation induces rapid apoptotic change, which may play an important role in the pathogenesis of radiation-induced pathologic conditions.
Kim, Dahee;Joo, Minhee;Lee, Dabin;Nguyen, Huu-Quang;Kim, Jeongkwon
Mass Spectrometry Letters
/
v.10
no.3
/
pp.79-83
/
2019
Horse heart myoglobin (MYG) and bovine serum albumin (BSA) were hydrolyzed by microwave-assisted weak-acid hydrolysis for 10, 20, 30, 40, 50, and 60 min using 2% formic acid (FA) at $100^{\circ}C$. Generally, the number of identified peptides increased with increasing irradiation time, indicating that the duration of microwave irradiation is linked to the efficiency of hydrolysis. For MYG, irradiation for 60 min provided the highest number of identified peptides, the greatest sequence coverage values and the highest MASCOT score values among the investigated irradiation times. Irradiation of BSA for 50 min, however, yielded a greater number of peptides than irradiation for 60 min due to the generation of miscleaved peptides after microwave irradiation for 50 min.
The purpose of this study was to suggest the use of laser energy in the the field of operative dentistry without considerable pulpal damage and significant effects on the dental hard tissue, additionally to find out the methods which could control the temperature rise. The laser beam (CW $CO_2$ laser, output: 6W, beam diameter: 1.5mm) was focused on the center of the occlusal surface of extracted lower molars. A Ge lens (focal length 200mm) was used to focus the primary laser beam. In order to vary the total amount of the same irradiated energy, experimental subjects were devided into three groups: continuously irradiated group, intermittently irradiated group, and water-cooled group after continuous laser irradiation. Temperature changes in the pulp chamber after laser irradiation were measured and recorded by the digital thermometer and recorder. The following results were obtained: 1. Temperatures in the pulp chamber were raised up in the order of the continuously irradiated group, intermittently irradiated group, water-cooled group after continuous laser irradiation. 2. In the continuously irradiated group, the temperature was raised up $1.7^{\circ}C$, $3.8^{\circ}C$, $7.3^{\circ}C$, $17.2^{\circ}C$ after 2, 4, 8, 16 seconds of the irradiation of laser. In the intermittently irradiated group, the changes were $1.2^{\circ}C$, $3.4^{\circ}C$, $6.3^{\circ}C$, $11.1^{\circ}C$, respectively. In the water-cooled group after continuous laser irradiation, the changes were $0.0^{\circ}C$, $0.8^{\circ}C$, $1.6^{\circ}C$, $6.9^{\circ}C$, respectively. 3. The starting time of temperature rise in the pulp chamber had no connection with laser irradiation time.
Journal of Korean Academy of Oral and Maxillofacial Radiology
/
v.20
no.1
/
pp.125-133
/
1990
It is known that radiation therapy is a kind of treatment choices of the maxillofacial tumors. This study is designed to investigate the effects of irradiation on rat's tongue tissues as functional tissues which relate to taste, mastication, and pronunciation. 88 rats (Sprague Dawley branch, male) were divided into control group of 4 and experimental group of 84. Experimental group was singly exposed to Co-60 irradiation with 8, 13, 18 Gy in the head and neck region. Animals were sacrificed on 1 hour, 3 hours, 6 hours, 1day, 3 days, 7 days, and 28 days after the irradiation. The specimens were observed by histopathological examination employing H-E stain and Van-Gieson stain. The results were follows; 1. The tongue tissue were severely swollen on the 1 hour after irradiation, but gradually decreased in course of time. 2. The basal cells of epithelium of tongue proliferated at initial stage of irradiation, but gradually decreased. The Keratin layer were gradually increased. 3. The muscles within the tongue were severely degenerated at initial stage of irradiation, but gradually recovered almost normally. 4. The tissue changes after irradiation were gradually increased by the degree of irradiation.
The polygenic variation in flowering time of soybean irradiated with Co$^{60}$ gamma ray evaluated in the R$_2$ generation. The genetic variance in the irradiation treatment was about four times that of the control. The basic possibility of selection for the early and the late in flowering time of soybean after irradiated with Co$^{60}$ was suggested in this paper.
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