• Horticultural Science & Technology
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• v.31 no.1
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• pp.56-64
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• 2013

Recent unusual weather due to global warming causes shortage of daily sunlight and constitutes one of the primary reasons for agricultural damages. LED light sources are frequently utilized to compensate for the shortage of sunlight in greenhouse agriculture. The present study is aimed at evaluating formations of phytochemicals as well as growth characteristics of mature strawberry fruits ('Daewang' cultivar) during cultivation in a closed growth chamber equipped with artificial LED light as a sole light source. Each LED light of blue (448 nm), red (634 and 661 nm) or mixed blue plus red (blue:red = 3:7) was separately supplied and the intensity of each light was adjusted to $200{\pm}1{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ at plant level with a photoperiod consisted of 16 hours light and 8 hours darkness. Strawberries grown under mixed LED light of blue and red wavelengths showed a higher production of fruits than those grown under other LED treatments. Fructose, one of the free sugars, increased in mixed LED light-grown fruits. Anthocyanin contents were elevated remarkably in the mixed LED light-grown fruits compared with those in other LED treatments. Contrastingly, contents of total phenolics and flavonoids were not of much different from one another among the fruits treated with various LED lights. On the other hand, ripening of strawberry fruits was found to be faster when grown under blue LED light compared with other LED treatments. Moreover, antioxidant activities of blue or red LED light-grown fruits, respectively, were significantly higher than those of mixed LED light-grown fruits. We suggest that when daylight is in shortage during cultivation in a greenhouse, supplementation of sunlight with LED light, which is composed of blue and red wavelengths, could be useful for the enhancement of productivity as well as of free sugar content in strawberry fruits. In addition, for the strawberry culture in the plant factory, selective adoption of LED light wavelength would be required to accomplish the purpose of controlling fruit maturation time as well as of enhancing contents of sugars and antioxidants of fruits.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.32-37
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• 2013

Relationships among climate changes, early frost, and fruit growth were studied during the final month to harvest of late-maturing 'Fuyu' persimmon (Diospyros kaki) to assess the changes in fruit characteristics during this critical period. The heavy frost on Nov. 16 defoliated more than 70% of the leaves, but with little damage on the fruits. However, all the leaves were defoliated by the heavy frost on Nov. 20, and all the fruits were cold-damaged by $-3.3^{\circ}C$ on Nov. 21. Fruit weight increased by 8-25 g per week from Oct. 25 (142 days after full bloom) to Nov. 15, reaching to 250 g, but it decreased by 3-4 g per week after the frost. Hunter a value of fruit skin gradually increased until the last harvest on Nov. 29 with a temporary halt in early Nov. when temperature was high, whereas fruit firmness rapidly decreased after the frost on Nov. 21. Fruit soluble solids were $15.7-16.1^{\circ}Brix$ for the final month. When some branches were covered with non-woven fabrics to avoid direct contact with frost, the fruits on the branches were not visually damaged by the low temperature although 40-60% of their leaves were defoliated on Nov. 16. However, low temperature on Nov. 20 and 21 defoliated all the leaves, causing cold damage on the fruits. There was a highly significant correlation between the fruit diameter and its weight ($R^2$ = 0.73-0.91). So, the regression equations could be used to estimate weight from diameter of the fruits sampled from the branches with the non-woven fabrics. The calculated fruit weight reached to a maximum of 240 g on Nov. 15. Daily increases in fruit weight were 1.1-2.5 g from Oct. 25 to 31, 1.9-3.5 g from Oct. 31 to Nov. 7, and 1.4-1.6 g from Nov. 7 to 12. However, fruit weight decreased by 0.3-1 g per day after the cold damage on Nov. 21. The results indicate that the most appropriate harvest time could be dependent on relationship of fruit growth to climate.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.80-88
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• 2013

Carrot (Daucus carota L. var. sativa) is one of the most extensively used vegetable crops in the world and a significant source of nutrient because of its high content of ${\beta}$-carotene, well known as the precursor of vitamin A carotenoid. However, seed-hairs generated and elongated from the epidermal cell of seeds inhibit absorption and germination by various factors such as carotol and so on. Accordingly, mechanical hair removal process is essential before commercialization of carrot seeds. Because of this process, producers will have additional losses such as time consuming, manpower, capital and so on. Furthermore, physical damage of seeds causes irregular germination rate. To overcome such cumbersome weaknesses, new breeding program for developing hairless-seed carrot cultivar has been needed and studies for molecular markers related to seed-hair characteristic is needed for a new breeding program. Therefore, in this study, cDNA libraries from seeds of short-hair seed phenotype CT-SMR 616 OP 659-1 line, hairy-seed phenotype CT-SMR 616 OP 677-14 line and short-hair seed phenotype CT-ATR 615 OP 666-13 line, hairy-seed phenotype CT-ATR 615 OP 671-9 were constructed, respectively. Furthermore, 1,248 ESTs in each line, total 4,992 ESTs were sequenced. As a result, 19 SNP sites and 14 SNP sites in each of 2 combinations were confirmed by analyzing these EST sequences from short-hair and hairy-seed lines. Then we designed SNP primer sets from EST sequences of SNP sites for high resolution melting (HRM) analysis. Designed HRM primers were analyzed using hairy seed phenotype CT-SMR 616 OP 1040 line and short-hair seed phenotype CT-SMR 616 OP 1024, 1025, 1026 lines. One set of HRM primers showed specific difference between the melting curves of hairy and short-hair seed phenotype lines. Based on this result, allele-specific (AS) PCR primers were designed for easier selection between hairy-seed carrot and hairless seed carrot. These results of HRM and AS-PCR are expected to be useful in breeding of hairless seed carrot cultivar as a molecular marker.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.531-537
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• 2013

The average annual and winter ambient air temperatures in Korea have risen by 0.7 and $1.4^{\circ}C$, respectively, during the last 30 years. Radish (Raphanus sativus), one of the most important cool season crops, may well be used as a model to study the influence of climatic change on plant growth, because it is more adversely affected by elevated temperatures than warm season crops. This study examined the influence of transplanting time, nitrogen fertilizer level, and climate parameters, including air temperature and growing degree days (GDD), on the performance of a radish cultivar 'Mansahyungtong' to estimate crop growth during the spring growing season. The radish seeds were sown from April 24 to May 22, 2012, at internals of 14 days and cultivated with 3 levels of nitrogen fertilization. The data from plants sown on April 24 and May 8, 2012 were used for the prediction of plant growth as affected by planting date and nitrogen fertilization for spring production. In our study, plant fresh weight was higher when the radish seeds were sown on $24^{th}$ of April than on $8^{th}$ and $22^{nd}$ of May. The growth model was described as a logarithmic function using GDD according to the nitrogen fertilization levels: for 0.5N, root dry matter = 84.66/(1+exp (-(GDD - 790.7)/122.3)) ($r^2$ = 0.92), for 1.0N, root dry matter = 100.6/(1 + exp (-(GDD - 824.8)/112.8)) ($r^2$ = 0.92), and for 2.0N, root dry matter = 117.7/(1+exp (-(GDD - 877.7)/148.5)) ($r^2$ = 0.94). Although the model slightly tended to overestimate the dry mass per plant, the estimated and observed root dry matter and top dry matter data showed a reasonable good fit with 1.12 ($R^2$ = 0.979) and 1.05 ($R^2$ = 0.991), respectively. Results of this study suggest that the GDD values can be used as a good indicator in predicting the root growth of radish.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.565-572
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• 2013

The objective of this study was to describe and analyze the effect of high pressure sodium lamp lighting (HPS) on dynamics of growth and dry matter partitioning, and light use efficiency of sweet pepper crop grown over winter season. Young sweet pepper seedlings were planted at 3.75 plants per $m^2$ on September 29, 2010 and treated with HPS for 16 hours from December 14, 2010 until March 18, 2011. The number of leaves per plant were significantly increased with HPS, whereas the number of internodes and leaf area were less affected. HPS reduced the plant height with higher number of fruits per stem compared to those of without HPS lighting (CON). There were large differences in total dry mass production, stem and fruit dry mass between HPS and CON and those with HPS increased by 67.8%, 28.5%, and 97.1% compared to CON, respectively. Each organs of dry mass partitioning was calculated by leaf, stem or fruit growth rate divided by total plant growth rate. Dynamics of dry mass partitioning to leaf and stem between HPC and CON was measured in range of 45-47% at beginning of growth phase and drastically decreased after starting fruit growth in both treatments. Dry matter partitioning to vegetative organs was 4% higher compared to the plant grown under HPS lighting. Averaged dry matter partitioning to fruit with HPS, however, was largely increased by 14.2% compared to CON. Dynamics of the plant growth were well described by expolinear growth equation with three parameters of maximum relative growth rate, absolute growth rate and lost time to reach linear phase. The maximum growth rate of leaf, stem and fruit with HPS was increased by 18.6%, 74.7%, and 143.5% compared to CON. There was a linear relationship between intercepted light integral and vegetative organs (leaf and stem), fruit or total dry mass production. Light use efficiency (LUE, $g{\cdot}MJ^{-1}$) of total dry mass was $4.90g{\cdot}MJ^{-1}$ for HPS and $3.84g{\cdot}MJ^{-1}$ for CON, LUE of vegetative organs was $1.56g{\cdot}MJ^{-1}$ for HPS and $1.61g{\cdot}MJ^{-1}$ for CON and LUE of fruit dry mass was $3.34g{\cdot}MJ^{-1}$ for HPS and $2.23g{\cdot}MJ^{-1}$ for CON. The difference in LUE of total dry mass between treatments, therefore, occurred mainly from the different in LUE of fruit dry mass.

• Korean Journal of Ecology and Environment
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• v.36 no.2 s.103
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• pp.124-138
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• 2003

This paper aimed to analyze the relationship between alga3 bloom patterns and hydrological, limnological data which were collected from major reservoirs in Korea for 8 years (1990${\sim}$1997). Water temperature of river-type reservoirs showed wider seasonal fluctuations than that of lake-type. pH of lake-type reservoirs was low in winter season but high in summer season. In contrast, river-type reservoirs showed high pH in spring and autumn seasons as well, and very low in summer season. COD of lake-type reservoirs and Paldang reservoir was lower (2${\sim}$3 mg/L) than that of Geumgang and Nagdonggang reservoirs (6${\sim}$9 mg/L). Dissolved oxygen (DO) of river-type reservoirs was higher than that of lake-type reservoirs. Seasonal fluctuation pattern of DO saturation in river-type reservoirs was high (80 ${\sim}$100%) and remained relatively constant whereas lake-type reservoirs showed the highest level (93%) in late spring or early summer, which gradually decreased entering winter season(46${\sim}$06%). And monthly variation of DO saturation showed inverse proportion to water volume in lake-type reservoirs. Nutrients concentration in river-type lake is higher than lake-type. Seasonal fluctuation of nutrients (T-N, T-P) in lake-type reservoirs was relatively small than that of river-type reservoirs. Annual mean N/P mass ratio of lake-type reservoirs was higher than that of river-type. Transparency tended to related with the suspended solid concentration in river-type reservoirs. Algal bloom of lake-type and river-type reservoirs occurred at any time except rainfall and winter periods. And it dominated in summer and early autumn, respectively. Algal bloom of river-type reservoirs was higher than that of lake-type. Relationship between rainfall and chlorophyll- a in lake-type reservoirs was relatively high, however river-type reservoirs showed insignificant.

• Korean Journal of Ecology and Environment
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• v.37 no.3 s.108
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• pp.344-354
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• 2004

A pilot study was performed from September 2000 to April 2004 to examine the feasibility of the wetland-pond system for the agricultural reuse of reclaimed water. The wetland system was a subsurface flow type, with a hydraulic residence time of 3.5 days, and the subsequent pond was 8 $m^3$ in volume (2 m ${\times}$ 2 m ${\times}$ 2 m) and operated with intermittent-discharge and continuous flow types. The wetland system was effective in treating the sewage; median removal efficiencies of $BOD_5$ and TSS were above 70.0%, with mean effluent concentrations of 27.1 and 16.8 mg $L^{-1}$, respectively, for these constituents. However, they did often exceed the effluent water quality standards of 20 mg $L^{-1}$. Removal of T-N and T-P was relatively less effective and mean effluent concentrations were approximately 103.2 and 7.2 mg $L^{-1}$, respectively. The wetland system demonstrated high removal rate (92 ${\sim}$ 90%) of microorganisms, but effluent concentrations were in the range of 300 ${\sim}$ 16,000 MPN 100 $mL^{-1}$ which is still high for agricultural reuse. The subsequent pond system provided further treatment of the wetland effluent, and especially additional microorganisms removal in addition to wetland-pond system could reduce the mean concentration to 1,000 MPN 100 $mL^{-1}$ from about $10^5$ MPN 100 $mL^{-1}$ of wetland influent. Other parameters in the pond system showed seasonal variation, and the upper layer of the pond water column became remarkably clear immediately after ice melt. Overall, the wetland system was found to be adequate for treating sewage with stable removal efficiency, and the subsequent pond was effective for further polishing. This study concerned agricultural reuse of reclaimed water using natural systems. Considering stable performance and effective removal of bacterial indicators as well as other water quality parameters, low maintenance, and cost-effectiveness, wetland- pond system was thought to be an effective and feasible alternative for agricultural reuse of reclaimed water in rural area.

• Korean Journal of Ecology and Environment
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• v.48 no.2
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• pp.108-114
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• 2015

Single - and combined effects of a domestic freshwater bivalve Unio douglasiae (7.6~8.6 cm in shell length) and zooplankton Daphnia magna (1~2 mm in body size) were examined to understand whether they inhibit the growth of harmful cyanobacterial bloom (i.e. Microcystis aeruginosa) in a eutrophic lake. The experiments were triplicated with twelve glass aquaria (40 L in volume); three aquaria without mussel and zooplankton, served as a control, three zooplankton aquaria (Z, density=40 indiv. $L^{-1}$), three mussel aquaria (M, density=0.5 indiv. $L^{-1}$), and three mussel plus zooplankton aquarium (ZM, density=40 indiv.Z $L^{-1}$ plus 0.5 indiv.M/L), respectively. Algal growth inhibition (%) calculated as a difference in the concentration of chlorophyll-a (Chl-a) before and after treatment. Chl-a in all aquaria decreased with the time, while a greatest algal inhibition was seen in the ZM aquaria. After 24 hrs of incubation, Chl-a concentration at the mid-depth (ca. 15 cm) in ZM aquaria reduced by 90.8% of the control, while 63.2% and 79.8% in Z and M aquaria, respectively. Interestingly, during the same period, the surface Chl-a was diminished by 51.9% and 65.4% relative to the control in Z and ZM aquaria, while 27.4% of initial concentration decreased in M aquarium, respectively. These results suggest that 1) this domestic freshwater filter-feeding bivalve plays a significant role in the control of cyanobacterial bloom (M. aeruginosa), and 2) the combination with zooplankton and mussel has a synergistic effect to diminish them, compared to the single treatment of zooplankton and mussel.

• Korean Journal of Ecology and Environment
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• v.40 no.3
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• pp.403-411
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• 2007

The purpose of the study was to evaluate fish community, based on conventional at Shannon-Weaver diversity index (H'), and ecological health, based on the Index of Biological Integrity (IBI) using fish assemblage in the eight sites of Nakdong River during June${\sim}$August 1999. Total number of species sampled was 19 species, and two sensitive species of Zacco temminckii (51%) and Rhynchocypris oxycephalus (28%) dominated the fish community. Also, trophic guild analyses showed that insectivore was 87% of the total and omnivore was rare, indicating that the ecological health is well maintained in the system. The pattern of spatial variation in the diversity index(H') was very similar to patterns of the species number and individual number, whereas the pattern of H' was not matched with the tolerance and trophic guild data. The diversity index (H') showed highest (1.56) in Site 6 where the proportion of sensitive species and tolerant species was minimum and maximum, respectively, and where the insectivore and omnivore were minimum and maximum. In other words, the diversity index was not matched at all with the trophic and tolerant guilds, indicating that the conventional index did not reflect the ecological characteristics of fish community in the system. In the mean time, the ecological health (IBI) averaged 33.5 (n=8), indicating "good${\sim}$fair condition" and the IBI values matched with trophic and tolerance guilds. Maximum IBI occurred in Site 2 where the sensitive and msectivore species were nearly maximum, and the tolerant and omnivore species were almost minima, indicating that IBI values were closely associated with the ecological functions and health conditions. Overall data suggest that the conventional diversity index may not effective for a evaluation of fish community, and that in contrast the IBI approach may be a useful tool for diagnosis of stream community.

• Journal of Animal Environmental Science
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• v.9 no.1
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• pp.9-18
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• 2003

Most of broiler houses in Korea have the trouble of environmental control such as suitability, stability, and uniformity of internal climate, resulting in serious stress on chickens. Accordingly, it is very urgent to develop optimum designs of naturally and mechanically ventilated broiler houses for Korean climate. In this study, the internal climates such as air temperature, humidity, dust, ammonia gas, and air velocity were measured at a naturally ventilated broiler house. The data were collected during summer season including local weather data. It was found that the difference between measured and optimum air temperatures was $14.0^{\circ}C$ in maximum during the summer time. The daily maximum range of internal averaged air temperature was found $10.5^{\circ}C$ while the uniformity was $5.2^{\circ}C$ in maximum. The maximum, average, and minimum internal averaged relative humidity were 89.3%, 73.7%, and 49.2%, respectively while locally measured were 95.1% and 47.2%, respectively in maximum and minimum. Considering Temperature-humidity index, during summer season, over 97% of totally rearing period was shown that counter plan is needed for thermal stress while it was very dangerous situation for 22% of the rearing period. The ammonia gas and dust concentrations were seriously affected by the broilers activity, growth level, and relative humidity.