• Title/Summary/Keyword: tillering

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Xanthomonas oryzae pv. oryzae triggers complex transcriptomic defense network in rice

  • Nino, Marjohn;Nogoy, Franz M.;Song, Jae-Young;Kang, Kwon-Kyoo;Cho, Yong-Gu
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.164-164
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    • 2017
  • High throughput transcriptome investigations of immunity in plants highlight the complexity of gene networks leading to incompatible interaction. To identify genes crucial to resistance against Xanthomonas oryzae pv oryzae, functional genetic analysis of selected differentially expressed genes from our microarray data set was carried out. A total of 13 overexpression vector constructs were made using 35S CaMV promoter which drive constitutive expression in rice. Most of the genes are developmentally expressed especially during maximum tillering stage and are commonly highly expressed in the leaves. When screened against Xoo strain K2, the transgenic plants displayed shorter lesion length compared with wild type Dongjin which indicates partial resistance. The levels of ROS continuously magnified after inoculation which indicates robust cellular sensing necessary to initiate cell death. Elevated transcripts levels of several defense-related genes at the downstream of defense signal network also corroborate the phenotype reaction of the transgenic plants. Moreover, expression assays revealed regulation of these genes by cross-communicating signal-transductions pathways mediated by salicylic and jasmonic acid. These collective findings revealed the key immune signaling conduits critical to mount full defense against Xoo.

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Overexpression of twin-arginine translocation (TAT) pathway conferred immunity to Xanthomonas oryzae v. oryzae in rice

  • Nino, Marjohn C.;Song, Jae-Young;Nogoy, Franz Marielle;Kang, Kwon-Kyoo;Cho, Yong-Gu
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.166-166
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    • 2017
  • OsTAT encodes a twin-arginine translocator (TAT) pathway signal protein. It contains a TRANS membrane domain and a chloroplast transit peptide. mRNA transcription profiling of OsTAT1 revealed that it is highly overexpressed in the leaves corroborating reports on its role in chloroplast. Moreover, its level of expression is more pronounced during earlier stages (germination, 3-leaf stage, and maximum tillering) of growth in rice. A lower disease progress curve of bacterial blight is evident in transgenic lines compared with the wild type, Dongjin indicating its involvement in immunity to Xoo. Expression pattern following infection of Xoo strain K2 depicts highest levels at 4 and 8 hour post-inoculation which implies crucial induction of resistance during early response. This study initially reports a new overview on the biological functions of plant's TAT pathway. Further molecular and genetic analyses are underway to provide detailed involvement of OsTAT in disease resistance.

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Varietal Difference of Resistance to Ozone Injury in Rice Plant (벼 품종별 오존 피해 저항성 차이)

  • 손재근;이상철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.3
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    • pp.338-343
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    • 1997
  • The response of seventy-five rice cultivars to ozone (O$_3$) were tested in the open-top chamber with ozone producing and monitoring system to determine the varietal difference of resistance to $O_3$ stress. Ozone was produced by electrostatic discharge in oxygen and was monitored by UV absorption ozone analyzer. Difference in response of rice to ozone was more clearly appeared on rice plants treated for 2 to 4 hours at 0.3 ppm concentration of $O_3$. Varietal resistance of rice to ozone was more distinctly classified at 21- to 35-day seedlings compared with 14-day rice plants. Most of indica and Tongil(indica$\times$japonica) type rice cultivars were more resistant than that of japonica cultivars based on the leaf injury to $O_3$. Eight Korean cultivars belong to japonica groups showed highly resistant reaction to $O_3$. Ozone exposure during booting stage caused lower grain fertility than exposures during seedling, maximum tillering and heading stages of rice.

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Morphological Alterations of Flower Induced by Chilling Stress in Rices

  • Hwang, Cheol Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.2
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    • pp.171-175
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    • 1999
  • Morphological alteration of floral organ development in rice affected by chilling stress was examined. Three varieties of rice were grown under natural conditions and subjected to 12$^{\circ}C$ for 3 or 6 days starting from the ineffective tillering stage, before heading stage and returned to natural condition. Headings were delayed by a 6 day chilling treatment. After heading the panicles were collected and examined for any possible alteration in floral organ development. It appears that there were some differences in sensitivity to chilling stress and degree of injury depending on treatment stages and variety. Chuchungbyeo was the most frequent in producing abnormal flowers among the three varieties examined. Meiosis stage was shown to be the most vulnerable to chilling stress in both Chuchungbyeo and Ilpumbyeo and young panicle differentiation stage was the frequent stage to alter flower development in response to chilling stress only in Chuchungbyeo. It was confirmed that abnormalities occurred in pollen due to chilling stress is a major factor leading to low yield, but to some extent the alterations in carpel development may playa certain role in determining a total yield in response to chilling stress at the reproduction stage in rice. There were abnormalities like extra stigmata, extra lemma, double ovary as well as abnormal anther formation in response to chilling stress. Further studies of the phenocopy observed in rice floral development may be useful for an understanding of the resistance against chilling injury during reproductive stages in rice.

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Effects of carbonized rice hull and wood vinegar on the improvement of cultivation condition and grain quality of rice

  • Cho, Sun-Sik;Heo, Kyu-Hong;Seo, Pil-Dae;Rico, Cyren;Bequillo, Irvin;Kang, Mi-Young;Lee, Sang-Chul
    • Proceedings of the Korean Society of Organic Agriculture Conference
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    • 2009.12a
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    • pp.317-317
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    • 2009
  • The effects of environment-friendly materials carbonized rice hull and wood vinegar on the improvement of rice quality and soil fertility were investigated. Combined application of carbonized rice hull and chemical fertilizer resulted in lower protein in rice, similar amylose content and generally higher palatability values. Combined application of wood vinegar and chemical fertilizer obtained high protein and amylose contents, and palatability values. However, both carbonized rice hull and wood vinegar did not exhibit weed control. In the carbonized rice hull treatments, soil K was high during heading stage while soil pH during harvest stage was low. In the case of wood vinegar treatments, clear distinction between total K and Ca was observed. K was high during tillering stage while Ca was high until harvest stage.

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Estimation of Rice Growth Using RADARSTA-2 SAR Images at Seosan Region

  • Kim, Yihyun;Hong, Sukyoung;Lee, Kyoungdo;Jang, Soyeong
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.4
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    • pp.237-244
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    • 2013
  • Radar remote sensing is appropriate for monitoring rice because the areas where this crop is cultivated are often cloudy and rainy. Especially, Synthetic Aperture Radar (SAR) can acquire remote sensing information with a high temporal resolution in tropical and subtropical regions due to its all-weather capability. This paper analyzes the relationships between backscattering coefficients of rice measured by RADARSAT-2 SAR and growth parameters during a rice growth period. We examined the temporal variations of backscattering coefficients with full polarization. Backscattering coefficients for all polarizations increased until Day Of Year (DOY 222) and then decreased along with Leaf Area Index (LAI), fresh weight, and Vegetation Water Content (VWC). Vertical transmit and Vertical receive polarization (VV)-polarization backscattering coefficients were higher than Horizontal transmit and Horizontal receive polarization (HH)-polarization backscattering coefficients in early rice growth stage and HH-polarization backscattering coefficients were higher than VV-polarization backscattering coefficients after effective tillering stage (DOY 186). Correlation analysis between backscattering coefficients and rice growth parameters revealed that HH-polarization was highly correlated with LAI, fresh weight, and VWC. Based on the observed relationships between backscattering coefficients and variables of cultivation, prediction equations were developed using the HH-polarization backscattering coefficients.

Inheritance of Scentedness of Rice Leaf in a Scented Breeding Line 'P-33-C-19' (향도의 방향성 유전)

  • 박순직
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.1
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    • pp.11-14
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    • 1984
  • The inheritance of scent in rice leaf and its genetic association with base color, hull color and resistance to bacterial leaf blight were studied in F$_2$ population of two rice crosses, P-33-C-19 (scented)/Zhu-Lian-Ai and P-33-C-19/Kataktara DA2. The scent was identified by leaf analysis at tillering stage. The scented and non-scented plants segregated in the ratio of 1scented: 3non-scented showing that a recessive gene responsible for the expression of scentedness. The scentedness was independently segregated with base color. hull color and resistance to bacterial leaf blight.

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Influence of Turbidity of Submerged Water on Photosynthetic Rate of Rice Plants (관수 탁도가 벼 광합성능에 미치는 영향)

  • 강양순;정근식;손양;김재철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.4
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    • pp.350-352
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    • 1988
  • This study was carried out to know the influence of turbidity of submerged water on photosynthetic rate of rice plant after water submerging treatment. Rice plants were transplanted in the pot at maximum tillering stage and they were sumberged for 3days at meiotic stage, 20days after transplanting, in the plastic container which had the clear and turbid water temperature adjusted around 24 to $25^{\circ}C$. Photosynthetic rate at 6 hours recovery after submergence was 41.5 to 54.2% compared to the control, but in the case of cultivar 'Nagdongbyeo', it was rapidly increased by 97.3 to 104.6% in the clear water and by 68.6 to 77.5% in the turbid water at 2 to 4 days recovery after submergence. Photosynthetic rates per plants at 6 hours to 4 days recovery after submergence were 128.3 to 245.5 $C_2$ mg.hr.$^{-1}$, in 'Samgamgbyeo' and 71.1 to 162.4 $CO_2$, mgㆍhr.$^{-1}$ in 'Nagdongbyeo' Photosynthetic rate to respiration ratio of rice plant recovery after submergence was lower compared to control plant and it was lower in 'Nagdongbyeo' than that in 'Samgangbyeo'.ngbyeo' than that in 'Samgangbyeo'.gangbyeo'.pos;.

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Susceptibility of Tongil type Rice Cultivar Milyang 30 previously Resistant to Xanthomonas campestris pv. oryzae ('밀양 30호'의 흰빛잎마름병 (백엽고병)이병화)

  • Choi Yong-Chul;Yun Myung-Soo;Sohn Jae-Kyun
    • Korean journal of applied entomology
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    • v.23 no.1 s.58
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    • pp.1-6
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    • 1984
  • This study was conducted to investigate the incidence of bacterial leaf blight (BLB) in Milyang 30 which was previously considered to be resistant to Xanthomonas campestris pv. oryzae Seeds of Milyang 30 were collected from Suweon, Milyang and Haenam. When rice plants from different source were inoculated at the maximum tillering stage and flag leaf stage, reactions of Milyang 30 were consistant. The susceptibility of Milyang 30 was found to be due to infection with tome isolates in the pathotype II of X. campestris pv. oryzae.

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Influence of Rice Root Nematode Population, Hirschmonniella imamuri, on Growth and Yield of Rice (벼뿌리선충이 벼의 생육 및 수량에 미치는 영향)

  • 조현제;최진식
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.2
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    • pp.195-201
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    • 1983
  • This study was conducted to find out the damage of rice root nematodes on rice and determine the economic injury level. In pot experiments, rice root nematode, Hirschmanniella imamuri, suppressed the growth and reduced yield of a rice, Milyang 23, above critical levels. Tillering was depressed above 5, 000 nematodes per pot inoculum levels. Heading was also delayed by 6 days when inoculum level was above 20, 000. Weight of roots and grain yield in rice plants which inoculated with 20, 000 nematodes/pot were reduced by 41% and 31%, respectively. Economic injury level of rice root nematode appeared to be 1, 500 nematodes per 30g of fresh root on Aug. 30.

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