• Korean Journal of Plant Resources
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• v.36 no.3
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• pp.225-236
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• 2023

Stomatal traits such as stomata density (SD), aperture length (APL) and width (APW), guard cell length (GCL) and width (GCW), and distance between stomata (DIS) were investigated to identify correlation with agronomic traits for 35 Korean wheat cultivars. Flag leaf width (FLW) of Korean wheat cultivars was the widest in Ol-mil, and the narrowest in Keumkang. SD tended to be higher on the adaxial side than on the abaxial side in Korean wheat cultivars. SD of adaxial and abaxial sides was classified into a cultivar with a significantly different or not. In APL, 18 wheat cultivars showed significant differences according to leaf side, and APL of adaxial was longer than APL of abaxial in 13 wheat cultivars. In APW, 15 wheat cultivars showed a significant difference, and APW of abaxial was wider than APW of adaxial among them. In GCL, 14 wheat cultivars showed a significant difference, and the GCL of abaxial was longer than the GCL of adaxial in 10 wheat cultivars. In GCW, 10 wheat cultivars showed a significant difference, GCW of adaxial was wider than GCW of abaxial and in 6 wheat cultivars. FLW of adaxial and abaxial showed a negative correlation with GCL and a positive correlation with grain number per panicle. FLW of only abaxial showed a positive correlation with DIS. The SD of the adaxial showed a negative correlation with GCL, while the SD of the abaxial showed a negative correlation with APL. APL of both sides of the leaf showed a positive correlation with GCL, and APW of only abaxial showed a negative correlation with GLC. DIS of adaxial showed a negative correlation with tiller number (TN), while DIS of abaxial showed a positive correlation with GNP.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.288-288
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• 2022

Rice is an important staple in the world. And drought is one of the important constraints that negatively affect yield loss and grain quality of rice. CRISPR/Cas9 is a new breeding strategy that can improve the characteristics of rice quickly and accurately. CRISPR/Cas9 is a novel approach that can reliably harvest rice yields in response to a rapidly changing climate. In addition, there is no externally inserted DNA left in genome-editing rice, and it is receiving attention as being able to take responsibility for future food because its characteristics are continuously improved. In the future, high levels of drought resistant in water-constrained environments will be required, which will reduce yield loss. OsSAP was genome-editing with CRISPR/Cas9 in rice. A different line number was assigned to each panicle, and the generation advanced by applying the ear-to-row method. Genome-editing rice has improved drought resistance in drought conditions. Also, in genome-editing rice, the target sequence was homozygous in the 0 generation, and the coefficient of variation of heading date, number of tiller, and 1,000-grain weight was very small in 2 generation. In the era of rapidly changing climate change, CRISPR/Cas9 presents a new breeding strategy that can rapidly and accurately improve agronomic traits of major food crops as well as rice. CRISPR/Cas9 is applied together with traditional breeding to develop into a new breeding strategy, it is suggested that food can be obtained stably in response to climate change.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.133-133
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• 2022

Temperature is a crucial environmental factor for rice cultivation due to the climate change and can influence the rice growth and development. Therefore, the effect of temperature on plant growth characters was examined during the vegetative stage. Plants were grown under three different temperatures: 23℃/13℃ for 18℃, 26℃/16℃ for 21℃ and 29℃/19℃ for 24℃ in the phytotron. The temperature was treated after transplanting and ended in early panicle initiation stage. Heading date of the two varieties were strongly affected by the temperature and were delayed in the 18℃. The plant height in the 18℃ was 21 % shorter than the 21℃ and 24℃ and the tiller and leaf number were increased in the 18℃. All the growth rates of the characters were the slowest in 18℃. The stem dry weight was significantly increased in 18℃. Nitrogen content was increased in the leaves of 18℃ whereas available phosphate and potassium content was found to be increased in the stems of 21℃ and 24℃. OsNRT 2.1 was overexpressed in the leaves and stems of 18℃ and OsNRT2.3a could be expressed in 18℃ and 21℃ temperatures whereas more expressed in 21℃. OsPT1 and OsPT6 could be expressed in the leaf of 18℃ and 24℃ but could be expressed in the stem of 18℃. OsHAK1 and OsHAK5 could be overexpressed in the leaves and stems of 18℃. For hormone, OsCKX2 gene was found to be up regulated in the leaves of 18℃ and OsIAA1 gene could be expressed more in the stem of 24℃.

• Korean Journal of Plant Resources
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• v.29 no.4
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• pp.511-518
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• 2016

The subject of this experiment is to supply basic data to inhibit non-productive tillers which are uneconomical for mechanical harvesting and to evaluate optimum planting density and sowing date in central district. Total number of tillers and effective first and second of tillers according to different planting density was higher in 80 ㎝ ridge than 60 ㎝ ridge in proso millet. The wider between plant distance on the ridge, the more increased total number of tillers and effective first and second of tillers. The highest effective tillers (91.7 %) in the first tillers was obtained from the second sowing date (23 May) among different sowing date and next is in the order of 3^rd(13 June, 89.8%) > 1^st(2 May, 85.6%) > 4^th(4 July, 85.2%). The percentage of effective tillers in the second tillers was decreased in the order of 2 May (53.7%), 23 May (40.7%), 13 June (22.2%), 4 July (0%) as the sowing date was delayed. There was no significant difference in days to heading and days to ripening according to different planting density. Although culm length was increased as planting density was increased, whereas number of tillers, stem diameter, ear length, grains per ear and 1000 grain weight was decreased. In the growth and yield characteristics of proso millet according to different sowing date, days to heading and days to ripening, culm length, stem diameter, ear length, grains per ear and yield per 10a were decreased. After the sowing date of 13 June, the reduction of growth and yield characteristics were higher because of excess-moisture injury.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.1
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• pp.49-58
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• 1992

The purpose of the experiment was to determine the effect of plant density and pattern on growth characteristics, total dry matter yield. protein yield and palatability of sorghum-sudangrass hybrid (Sordan 79). The main treatment was three levels of plant density (Skg, 15kg. 30kg/ha) and the sub treatment was two plant patterns of square and rectangular planting.The experiment was arranged as a split plot design and conducted on the Experimental Livestock Farm of Kon-Kuk Univ. in Chungju, 1989. The results obtained are summarized as follows :1. As the plant density was increased, plant length, leaf length, leaf width(P<0.05) and leaf number were gradually decreased, but in the same plant density, plant length, leaf length, leaf width and leaf number were generally increased in the rectangular plot. 2. In the first cutting time, the ratio of leaf was the highest at the rectangular plot of high density (30kg/ha, 25cm$\times$4cm), but in the second cutting time, the ratio of leaf was the highest at the square plot of medium density (15kg/ha, 14cm$\times$ 14cm). 3. The ratio of dead stubble after cutting was generally increased by increasing the plant density. Also, in the same planting density, the ratio of dead stubble was generally increased in the square plot. 4. The tiller number per plant tended to decreased by increasing thc plant density, and the tiller number was increased in the rectangular plot. In the same plant density. 5. Total fresh and dry matter yield were the highest at the rectangular plot of medium density (IS kg/ha, 33cm$\times$6cm) wholly, but the low density was shown the lowest.6. The crude protein content was increased by increasing the plant density per unit area(P<0.05) and the protein yield was also increased by increasing the planting density. 7. According to the increase of plant density, the stem was thinned, but NDF, ADF did not show different. The palatability was in the order of high (30kg/ha))medium (15kg/ha))low density(5kgiha) at the first cutting time, but it was in the order of high)low)medium density at the second cutting time.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.1
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• pp.19.2-19.2
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• 1992

The purpose of the experiment was to determine the effect of plant density and pattern on growthcharacteristics, total dry matter yield. protein yield and palatability of sorghum-sudangrass hybrid(Sordan 79). The main treatment was three levels of plant density (Skg, 15kg. 30kg/ha) and the subtreatment was two plant patterns of square and rectangular planting.The experiment was arranged as a split plot design and conducted on the Experimental Livestock Farm of Kon-Kuk Univ. in Chungju, 1989.The results obtained are summarized as follows :1. As the plant density was increased, plant length, leaf length, leaf width(P<0.05) and leaf number were gradually decreased, but in the same plant density, plant length, leaf length, leaf width and leaf number were generally increased in the rectangular plot.2. In the first cutting time, the ratio of leaf was the highest at the rectangular plot of high density(30kg/ha, 25cm$\times$4cm), but in the second cutting time, the ratio of leaf was the highest at the square plot of medium density (15kg/ha, 14cm$\times$ 14cm). 3. The ratio of dead stubble after cutting was generally increased by increasing the plant density. Also, in the same planting density, the ratio of dead stubble was generally increased in the square plot. 4. The tiller number per plant tended to decreased by increasing thc plant density, and the tiller number was increased in the rectangular plot. In the same plant density. 5. Total fresh and dry matter yield were the highest at the rectangular plot of medium density (ISkg/ha, 33cm$\times$6cm) wholly, but the low density was shown the lowest.6. The crude protein content was increased by increasing the plant density per unit area(P<0.05) and the protein yield was also increased by increasing the planting density. 7. According to the increase of plant density, the stem was thinned, but NDF, ADF did not show different. The palatability was in the order of high (30kg/ha))medium (15kg/ha))low density(5kgiha)at the first cutting time, but it was in the order of high)low)medium density at the second cutting time.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.3
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• pp.172-181
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• 2020

Koshihikari has been one of the most popular rice cultivars with good eating quality since the 1960s despite its susceptibility to blast disease and lodging. To map the genes controlling blast resistance and to develop promising blast-resistant breeding lines inheriting Koshihikari's high eating quality, a recombinant inbred line (RIL) population was developed from a cross between Koshihikari and a blast resistance donor with early maturity, Baegilmi. A total of 394 Koshihikari × Baegilmi RILs (KBRIL), and the two parents, were evaluated for blast resistance and major agronomic traits including heading date, culm length, panicle length, and tiller number. A linkage map encompassing 1,272.7 cM was constructed from a subset of the KBRIL (n = 142) using 130 single nucleotide polymorphisms. Two quantitative trait loci (QTL) for blast resistance, qBL1.1 harboring Pish/Pi35 and qBL2.1 harboring Pib, were mapped onto chromosomes 1 and 2, respectively. qBL1.1 was detected in both of the experimental sites, Namwon and Jeonju, while qBL2.1 was only detected in Namwon. qBL1.1 and qBL2.1 did not affect agronomic traits, including heading date, culm length, panicle length, and tiller number. From the 394 KBRILs, lines that were phenotypically similar to Koshihikari were selected according to heading date and culm length and were further divided into the following two groups based on blast resistance: Koshishikari-type blast resistant lines (KR, n = 15) and Koshishikari-type blast susceptible lines (KS, n = 15). Although no significant differences were observed in the major agronomic traits between the two groups, the KR group produced a greater mean head rice ratio than the KS group. The present study provides useful materials for developing blast-resistant cultivars that inherit both Koshihikari's high eating quality and Baegilmi's blast resistance.

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.3
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• pp.226-234
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• 1984

In order ti find out the optimum seeding time(OST) and optimum seed rate(OSR) of Italian ryegrass on seed production, this studies with tetraploid cv. Tetrone were carried out on the experimental field of Livestock Experiment Station. Treatments included seed rates of 1, 2, 3 and 4 kg per 10a and combined with seeding time on 20, 30 Aug, 9, 19 and 29, September 1983. Seeds were sown in rows 50 cm apart and were spaced in a continuous line with width of 15 cm within the rows. The results are summarized as follows: 1. Autumn tillers could be classified into three groups from winter-killing point of view, namely winter-killing completely, damaged growing point only and living tillers. 2. The young inflorescence-bearing stem in Italian ryegrass which were sown earlier than 9. September were more susceptible to winter killing. Tiller buds in those stems which originated from an axillary buds at the stem base within senescent leaf-sheaths emerged lately in spring, and consequently heading was delayed, culm length shortened and seed yield reduced. 3. Tiller buds which originated from damaged growing point only and living tillers in moderate shoots emerged early in spring and those tillers became mainly spike-bearing culm. 4. The emergence-time of tillers influenced on culm-, spike- length and ripenness more than seeding time and seed rate. 5. Seed yield was mostly affected by the number of spikes per unit area. 6. For the safety of over-wintering and enough spikes on seed production, OST and OSR at Suweon were the last part of September and 2-3 kg per 10a, respectively. Especially OSR was 2 kg per 10a for early and 3 kg per 10a for late OST.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.2
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• pp.87-92
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• 2015

This experiment was conducted to evaluate the effect of seeding methods and application levels of nitrogen fertilizer on the yield and forage quality of whole crop rice (WCR). The WCR variety "Namil" was directly seeded on April 25 and transplanted on May 25. Five levels of nitrogen fertilizer were applied (90, 110, 140, 170 and 200 kg/ha). There were no significant differences (p<0.05) of the emergence date, heading date and disease resistance based on the nitrogen fertilizer rates; however, the WCR became dark at higher nitrogen fertilizer rates. The plant height increased at higher nitrogen fertilizer rates and the tiller number showed the same trend. In contrast to a direct seeding method, transplanting increased the tiller number. The dry matter (DM) content did not show a certain tendency based on nitrogen fertilizer rates, while the fresh and dry matter yields increased with incremental changes of the nitrogen fertilizer rates (p<0.01), and the transplant method increased the yield size. In yield analysis, the plot direct-seeded with 140 kg N/ha and the transplanting with 170kg N/ha showed the highest yields. The crude protein (CP) content increased with higher nitrogen fertilizer rate, but there was no significant differences between transplant and direct-seeding methods. The content of ADF (acid detergent fiber) and NDF (neutral detergent fiber) increased with higher nitrogen fertilizer rate, but total digestible nutrient (TDN) content decreased with increased nitrogen levels. Although high nitrogen applications increased the fresh and DM yields, the 140 kg/ha nitrogen fertilizer level is recommended as the proper nitrogen fertilizer level, considering both yield and the environments.

• Weed & Turfgrass Science
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• v.6 no.1
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• pp.67-76
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• 2017

The study was initiated to evaluate the effects of overseeding warm-season grass (Zoysia japonica Steud.) with cool-season grasses (CSG) on turfgrass density, uniformity and tiller appearance and to determine turfgrass species and seeding rate applicable for a practical use. Treatments were comprised of Kentucky bluegrass (KB, Poa pratensis L.), perennial ryegrass (PR, Lolium perenne L.), tall fescue (TF, Festuca arundinacea Schreb.) and their mixtures. Overall turfgrass density and uniformity were much better with the overseeded treatments over the control. In early stage after overseeding, the greater the PR in treatments, the greater the turfgrass density and uniformity. But the higher the KB, the lower the density and uniformity. From the middle-stage evaluation, however, we observed the opposite results as compared with early-stage findings. Accordingly, the KB was highest in turfgrass density and uniformity, while the PR lowest. In regards of mixtures, both turfgrass density and uniformity were better with increased KB and decreased PR in overseeding rates. As for a medium-quality mixtures of Korean lawngrass with CSG, it would be the best choice to apply with KB at $50g\;m^{-2}$ and equal combination of KB, PR and TF by 1/3 in mixing at $75g\;m^{-2}$ in terms of sustainable density and uniformity.