Kim, Yung-Hi;Song, Dong-Keun;Wie, Myung-Bok;Song, Joon-Ho;Choi, Yeun-Sik
The Korean Journal of Pharmacology
/
v.26
no.1
/
pp.1-6
/
1990
We established an in vitro system of central serotonergic neurons by culturing dissociated rat embryonic (El4) brainstem cells to 14 days in vitro and monitored the serotonergic neuronal growth by measuring 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) contents in the cells with hish performance liquid chromatography with electrochemical detection (HPLC-EC). We studied also tile effects of various drugs on the contents of 5-HT and 5-HIAA, confirming in vivo reports. The 5-HT content (13 ng/mg protein) and 5-HT turnover rate (17 pmol/mg protein/h) at 14 days in vitro were in good agreement with those reported in the adult rat brain. The 5-HT content was more easily depleted with p-chlorophenylalanine, a tryptophan hydroxylase inhibitor than with NSD 1015 (3-hydroxybenzylhydrazine), an aromatic L-amino acid decarboxylase (AADC) inhibitor. Incubation of the cultures with tryptophan or 5-hydroxytryptophan increased the rate of serotonin formation implying that neither tryptophan hydroxylase nor AADC is saturated with its amino acid substrate in this in vitro system . The 5-HT content was depleted by reserpine. The 5-HT and 5-HIAA contents were increased and decreased, respectively, by monoamine oxidase inhibitors. All the above results indicate that the biochemical properties of the central serotonergic neurons in this culture system reflect reliably those of central serotonergic neurons in vivo. We suggest that measuring 5-HT and 5-HIAA contents in the primary cultured dissociated brainstem-cells with HPLC-EC is useful in the study of pharmacology as well as toxicolgy of the central serotonergic neurons.
The chemical changes and the quality of the soysauce which was Bade by new convenient method using vinyl teat instead of koji room, replacing wheat with milled barley koji were investigated. Protease activity of the koji was higher as tile ratio of soybeans was increased but amylase activity was higher as the ratio of milled barley was increased. The amounts of total nitrogen showed substantial increases until 80 days and afterwards, their rates of increases were lower. The more its content of soybeans, the higher its amount of total nitrogen, The amount of amino nitrogen increased slowly in all samples and the sample which had a large amount of soybeans was higher than those with small amounts of soybeans. The amount of ammonia nitrogen showed increases until 120 days tut decreased after then. The amount of reducing sugar showed increases until 80 days and decreased after then and the greater its source of carton, the higher its amount of reducing sugar. The total acid contort had large increases until 100 days in all samples and afterward they did not show any particular changes. The changes of pH decreased for 100 days and afterwards, they increased a little. In the original soysauce, combined ratio of soybean 3 to milled barley 2 and the sample of those ratio 4 : 1 are better than others and in diluted soysauce combined ratio of soybean 4 to milled barley 1 is better than the others. In original soysauce the use of $20{\sim}40%$ barley koji, which is added to soybeans, is superior and diluted soysauce the use of 20% barley koji is superior.
Watermarking is a widely employed method tn protecting copyright of a digital image, the owner's unique image is embedded into the original image. Strengthened level of watermark insertion would help enhance its resilience in the process of extraction even from various distortions of transformation on the image size or resolution. However, its level, at the same time, should be moderated enough not to reach human visibility. Finding a balance between these two is crucial in watermarking. For the algorithm for watermarking, the predefined strength of a watermark, computed from the physical difference between the original and embedded images, is applied to all images uniformal. The mean brightness or contrast of the surrounding images, other than the absolute brightness of an object, could affect human sensitivity for object detection. In the present study, we examined whether the detectability for watermark noise might be attired by image statistics: mean brightness and contrast of the image. As the first step to examine their effect, we made rune fundamental images with varied brightness and control of the original image. For each fundamental image, detectability for watermark noise was measured. The results showed that the strength ot watermark node for detection increased as tile brightness and contrast of the fundamental image were increased. We have fitted the data to a regression line which can be used to estimate the strength of watermark of a given image with a certain brightness and contrast. Although we need to take other required factors into consideration in directly applying this formula to actual watermarking algorithm, an adaptive watermarking algorithm could be built on this formula with image statistics, such as brightness and contrast.
PARK Joo-Suck;LEE Sam-Seuk;KANG Young-Shil;LEE Byung-Don;HUH Sung-Hoi
Korean Journal of Fisheries and Aquatic Sciences
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v.25
no.4
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pp.251-264
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1992
Distribution of indicator species of copepods and chaetognaths were studied as an indicator species of water mass in the southeastern area of the Yellow Sea. Undinula darwini, Lucicutia flavicornis, Pleuromamma gracilis, Euchaeta resselli, Euchaeta plane and Sagitta enflata were found to be reliable indicator species for determining warm water mass. Of these species, E. plana and E. rusrelli have a weak tolerance on the low temperature. Sagitta crassa was indicator species of neritic waters; Sagitta bedoti was that of mixing waters. Centropages abdominalis represented neritic cold waters. In February, U darwini, L. flavicornis, P. gracilis, E. russelli, E. plana and S. enflata occurred in the western waters of Cheju-Do where warm waters over $14^{\circ}C$ occupied. Centropages abdominalis occurred in the northern area beyond Chindo with water temperature less than $10^{\circ}C$. E. plana, E. russelli and S. bedoti were found at the regions between Cheju-Do and Chindo where the water temperature was $12- 14^{\circ}C$ corresponding to the mixing waters. Based on cluster analysis and T-S diagram in February three different water masses were identified from the south to the north. In August, water masses were analyzed at two different layers, 0-20m and 20m- bottom layers, separated by bhermocline depth. In 0-20m layer, E. plana and E. russelli were found from the western waters of Cheju-Do to Daehuksando. In 20m- bottom layer, E. russelli and E plena occurred at the northwestern waters of Cheju-Do with the water temperature warmer than $12^{\circ}C.\;C.$ abdominalis was found at the northern area beyond Chindo. Based on the cluster analysis and T-S diagram in August three different water masses at 0-20m and 20m-bottom layers were identified from the coast to the offshore. C. abdominalis was found at the adjacent water of Chindo at 0-20m layer and the northern area beyond Chindo at 20m~bottom layer. This fact suggested that the cold water mass existed at tile adjacent waters of Chindo in summer.
Gyeonggi metamorphic complex in the Gwangju area include banded biotite gneiss and quartzofeldspathic gneiss. Detailed structural analysis suggests that structural elements in the study area were formed by at least five phase of deformations. Penetrative compositional foliations(S1) formed in the banded gneiss during the first metamorphism and deformation (D1). After intrusion of plutonic rocks, the second deformation (D2) produced S2 foliations in the banded gneiss and quartzofeldspathic gneiss during the second metamorphism. D3 structures are represented by isoclinal folds (F3) whose axial surfaces are parallel to S3 foliations. The N-S oriented shortening (D4) was accommodated by closed upright F4 fold with about 100m of axial surface separation. F4 fold is refolded by regional F5 folding resulting in different orientation and fold style of F4 fold according to the position of F5 fold. The F4 fold with tight interlimb angle is subparallel to the axial surface (north-south) of F5 fold in the core of the F5 fold. In contrast the F4 fold trends northeast in the western limb and northwest in the eastern limb of F5 fold. The interlimb angle is larger in the limbs than that in the core of F5 fold. The trace of foliations is constrained by mainly F4 and F5 folds. Joint fanning around fold is developed in the limbs of F5 fold and bc joints are dominant in the hinge area of F5 fold. A strike-slip fault had developed in tile central part of the study area after F5 folding. The orientation of joint and foliation is rotated anticlockwise about $15^{\circ}$ by the landslide occurred during the Quaternary.
Journal of the Korean Society of Food Science and Nutrition
/
v.25
no.6
/
pp.986-992
/
1996
This study was designed to observe the effects of the feeding Platycodon grandiflorum(6 or 22 years) extract on the improvement of the blood glucose, lipids in the serum and liver of alloxaninduced hyperglycemic rats(S.D. strain, ♂), alloxan monohydrate 15mg/kg B.W./day I.P. injection) for 3 weeks. Concentrations of blood glucose were significantly higher in the alloxan administration(I.P.) groups(groups 2, 3 and 4) than those in the control group(group 1, basal diet). Blood glucose concentrations were remarkably lower in the group 3(basa1+alloxan+6years Platycodon grandiflorum) and 4(basa1+alloxan+22years Platycodon grandiflorum) than those in the group 2(basal+alloxan), and particularly, lower in the group 4. Concentrations of total cholesterol and LDL-cholesterol in serum were significantly lower in the groups 3, 4 than those in the group 2, and remarkably, lower in the group 4 than those in the group 3. Concentrations of HDL-cholesterol in serum were tile highest in the group 1. Those in the groups 3 and 4 were higher than those in the group 2. Atherosclerotic index were lower in the group 3, 4 than those in the group 2. In the alloxan-induced diabetic groups(groups 2, 3, 4), the serum free cholesterol, cholesterol ester, triglyceride and phospholipid concentrations were significantly lower in the group 3, 4 than those in the group 2. Contents of total cholesterol, triglyceride and phospholipid in liver were remarkably lower in the all experimental groups than those in the group 2. The activities of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase In serum were the highest in the group 2, but the other groups were rather lower. From the above research, the Platycodon grandiflorum(6 or 22 years) extracts were effective on the improvement of the blood glucose, lipid compositions in serum and liver. And particularly, Platycodon grandiflorum(22 years) was more effective than those in the Platycodon grandiflorum(6 years).
Journal of the Korean Society of Food Science and Nutrition
/
v.25
no.6
/
pp.1006-1015
/
1996
This study was carried out to investigate the effects of dietary calcium-salt, estrogen-treatment, and estrogen/calcium treatment on calcium, phosphorus and nitrogen metabolism. Female Sprague-Dawley rats with a body weight of 250~280g were underwent ovariectomy or sham-operation. The ovariectomized rats were divided into 9 different experimental groups including the saline-treated group, the estrogen-treated group, the high calcium salt-treated group, and the estrogen/calcium treated groups and fed for 6 weeks. Each group daily intake and excretion of calcium, phosphorus and nitrogen were measured and apparent digestibility and balance were also studied. The results were as follows: The excretion level of winn calcium was significantly higher the ovariectomized rats than the sham-operation group(p<0.01) and reduced with estrogen treatment but this difference was not statistically significant. Fecal loss of calcium was higher the ovariectomized rats than the sham-operation group(p<0.001). When the estrogen was treated, fecal loss was decreased and then apparent digestibility of calcium was increased. Calcium balance was significantly higher the high calcium treated groups than the control diet groups. The excretion level of urinary Phosphorus was higher the ovariectomized rats than sham-operation group, while these showed to be decreased in the calcium salt, the estrogen and the estrogen/calcium treated groups(p<0.01). Fecal loss of phosphrous was higher in the ovariectomized rats. When the estrogen was treated, the fecal loss was decreased in the avariectomized rat than that of other groups. The excretion level of urinary nitrogen was higher the ovariectomized rats than the sham-operation, while these showed to be decreased in the estrogen, the estrogen/calcium, and the estrogen gradually reduction/calcium intensification. Fecal loss of nitrogen was decreased in tile estrogen treated group. The results in this study showed that high calcium, estrogen/calcium and estrogen gradually reduction/calcium intensification in the ovariectomized rats enhanced calcium balance compared to the ovariectomized rats without calcium treatment, but little effects on the phosphorus and nitrogen balance.
Korean Journal of Construction Engineering and Management
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v.12
no.6
/
pp.65-78
/
2011
Construction business, which is complex and long-term business, requires accurate estimation and verification in construction costs and payment procedure from project planning to the completion of construction phase. And more importantly, it is necessary to investigate and determine the risk factors related to construction costs during the entire process including design planning, construction drawings, and quantity calculating. But, currently, it is not seem to be adequate to cope with the risk and increased construction costs against the operational budget in terms of actual costs when screening and estimating the bidding cost of public apartment. Therefore, this study selected and analyzed 40 sites' report of construction completion account from 2004 to 2010 focused on the adequacy on the modification of contract and design planning and on the complication of the budget in the beginning of the project. This study deducted various risk causes and results by analyzing actual costs according to year, architectural area, region, construction cost and sale/lease classification. We could find out construction risk according to annual variation of government policy and economy, and also deducted risk items by construction characteristic according to region and architectural area. Study result, we first found out the problems of lowest price award system according to the construction costs. The weight of the cost increase risk was analyzed that subcontract and material costs are very high. Roof and tile work were analyzed highly in subcontract cost risk and reinforcing bar and cement were analyzed highly in material cost risk, among direct construction cost. Finally, this study results could be used in comparing the categories of the construction costs made by specific construction process, belonging to the construction costs, with the operational budget made in the beginning of the project that can enable to grasp unpredictable risks over the construction costs and making quantitative analysis for it through analyzing the range of fluctuation and variations led by the fluctuations in the actual construction costs.
This experiment was carried out to investigate the penetration velocity and diffusion ability of methyl bromide fumigants under the condition of natural gravity, and the effect of in control of yellow corn in the vortical silo. The results were as fallows 1. The methyl bromide remainded concentration on the surface of the vertical silo was rapidly reduced from over 100mg/l to under 20mg/l, within 2 hours after supplying methyl bromide fumigant 2. Even 3m depth place from the surface of the vertical silo filled with yellolw corn was well penetrated within 30 minutes, but its remained methyl bromide gas was reduced gradually from over 100mg/l to 30mg/l after 12 hours duration. 5. In case of 13m place tile penetration ability of methyl bromide was over 100mg/l within one hour and kept the same concentration of it for 12 hours, its remainded methyl bromide preserved over 40mg/l until 48 duration time. 4. Less methyl bromide gas was detected at the bottom place than 3m and 13m depth of the vertical silo. It showed bellow 20mg/l of methyl bromide gas only through the whole duration time. 5. Comparing to the vertical penetration velocity of mehtyl bromide gas, the horizontal diffusion ability was not so good. Therefore, remained methyl bromide gas of 3m depth at the wall side of silo was about half than that of center circle where the nearer place from the methyl bromide supplying point, and the methyl bromide gas of 13m depth placed center circle was detected about 15 to 20 times than that of wall side. 6. For the purpose of examining the mortality of methyl bromide, the testing insects (Lesser Rice weevil and confused flour beetle) placed on the surface and bottom side of the silo were killed completely after 24 hrs fumigation, and the same results showed also at the 3m, 7m and 13m depth after 48 hrs fumigation.
This study is about the re-validation evaluation of the family Relationship Scale (FRS), developed to measure the family relationship in the social work practice. This study aims at re-validating the FRS, developed and validated in by Yang in 2001 for more general utilization. The sample was married mates and females residing in Seoul. For Face Validity, the content analysis was performed, and the FRS was re-validated in the dimensions of Love & Caring, Acceptance, and Recognition, positive affection, empathy, and autonomy and flexibility for each area. Internal reliability was .93, and internal consistency among three dimensions was 93%. For Empirical Validity, the Construct validity, the Criterion validity, and the Discriminant validity were performed. Construct Validity was validated through factor analyses. Commonalities for the factor analysis was 54%, and the factor loading for each factor was over .45. The confirmative factor analysis also confirmed the fitness of the scale. For Predictive Validity of Criterion Validity, regression analysis showed that the family stress scores became lower as the scores of the family relationship became higher; the discriminant analysis revealed that the family stress turned low ill tile group of high scores of family relationship. The Correlation analysis for Concurrent Validity was performed and the results showed the positive and significant relationship with a couple communication level (r=54) and a parent-child communication level (r=64). Life satisfaction and mental health level also revealed significantly positive correlation to prove Convergent Validity. Physical health level revealed a weak relationship with family relationship providing the evidence of Discriminant Validity. Discriminance was also proved by the analysis of variance with demographics. Thus, Cross Validation was confirmed the validation of the FRS through the various analyses with the married population. This study result improved the validity generalization of the Scale and verify the generalized usage of this sociometric scale in the field of social work practice.
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