• Mass Spectrometry Letters
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• 제4권3호
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• pp.47-50
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• 2013

An ultra-fast generic LC-MS/MS method was developed for high-throughput quantification of discovery pharmacokinetic (PK) samples and its reliability was verified. The method involves a simple protein precipitation for sample preparation and the analysis by ultra-fast generic LC-MS/MS with the ballistic gradient program and selected reaction monitoring (SRM) mode. Approximately 290 new chemical entities (NCEs) (over 10,000 samples) from 5 therapeutic programs were analyzed. The calibration curves showed good linearity in the concentration range of 1, 2 or 5 to 2000 ng/mL. No significant ion suppression was observed in the elution region of all the NCEs. When approximately 300 plasma samples were continuously analyzed, the peak area of internal standard was constant and reproducible. In the repeated analysis of samples, the plasma concentrations and the area under the curve (AUC) were consistent with the results from the first analysis. These results showed that the present ultra-fast generic LC-MS/MS method is reliable in terms of selectivity, sensitivity, and reproducibility and could be useful for high-throughput quantification and other bioanalysis in drug discovery.

• 대한교통학회지
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• 제24권7호
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• pp.115-128
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• 2006

도로용량편람(2004)에 제시된 합류부 분석 방법론은 수요가 용량을 초과할 경우 정체가 발생한다는 가정을 전제로 하고 있다. 그러나 많은 경우, 본선과 연결로 수요의 합이 용량을 초과하지 않은 상태에서도 합류부에서는 정체가 발생하고 있는 실정이며, 현재의 분석방법으로는 본선 및 연결로가 정체발생과 관련하여 어떠한 영향을 끼치는지에 대한 분석이 어려운 실정이다. 본 연구에서는 합류부 본선 및 연결로 수요교통량의 조합에 따라 발생할 수 있는 합류부 교통상태를 추정할 수 있는 모형을 개발하였다. 본 연구에서 제시하는 모형의 가장 큰 특징은 가변용량 개념의 도입인데 즉, 합류부 본선 및 연결로 수요교통량의 조합에 따라 정체되지 않고 통과될 수 있는 최대통과가능교통량 및 정체발생시의 최대통과교통량 산정에 있다. 이를 통해 합류부의 교통상태가 정체될 것인지, 또한 정체시 최대통과교통량 및 정체규모는 얼마가 될 것인지 추정할 수 있었다. 한편, 현재 사용하고 있는 합류부 서비스수준 평가기법은 실제 교통상황을 반영하지 못함으로 인해 고속도로 현장에서는 정체상황이 발생되고 있으나 수요가 용량을 초과하지 않았다는 이유로 정상교통류 상태로 평가하는 단점을 지니고 있다. 따라서 본 연구에서는 보다 현실적인 합류부 서비스수준 평가기법을 제시하기 위해 본 연구에서 개발한 합류부 분석방법론을 토대로 새로운 합류부 서비스수준 구분 기준을 제시하였다.

• 한국전자파학회논문지
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• 제19권7호
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• pp.741-753
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• 2008

본 논문에서는 IEEE 802.11a 무선 LAN의 이상적인 채널 환경과 페이딩 채널 환경에서 패킷의 페이로드 크기에 따른 MAC(Medium Access Control) 계층의 CSMA/CA(Carrier Sense Multiple Access/Collision Avoidance) 기반 DCF(Distributed Coordination Function) 처리율을 비교 분석하였다. 이상적인 채널 환경인 경우 에러가 없는 채널을 의미하고, 임의의 전송 주기 동안 패킷을 전송하는 단말이 1개만 존재하며, 다른 단말은 패킷을 수신한 후 응답한다고 가정한다. 페이딩 채널 환경인 경우 채널상에서 비트 에러는 랜덤하게 발생되며, 단말수 n은 고정되고, 각각의 단말은 항상 전송 패킷을 가지고 있는 포화 조건(saturation condition) 하에서 동작된다고 한다. IEEE 802.11a 무선 LAN의 처리율을 구하기 위해 기존 연구에서는 주로 이상적인 채널 환경을 가정하여 최대 처리율을 구하였는데, 실제의 통신 환경은 페이딩 패널이므로 본 연구에서는 $E_b/N_o$를 25 dB, 부 채널에서 직접 수신된 신호와 산란되어 수신된 신호의 전력비 $\xi$는 복합 Rayleigh/Ricean 페이딩을 고려하여 6으로 정하였다. 분석 결과, 이상적인 채널 환경에서의 처리율에 비교하여 페이딩 채널 환경에서의 처리율이 모든 페이로드 크기에서 더 작아진다는 것을 알 수 있으며, 전송율이 증가할수록 이상적인 채널의 최대 처리율에 대한 페이딩 채널의 포화 처리율의 감소 비율이 더 커진다는 것도 알 수 있다.

• BMB Reports
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• 제37권1호
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• pp.45-52
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• 2004

The goal of interaction proteomics that studies the protein-protein interactions of all expressed proteins is to understand biological processes that are strictly regulated by these interactions. The availability of entire genome sequences of many organisms and high-throughput analysis tools has led scientists to study the entire proteome (Pandey and Mann, 2000). There are various high-throughput methods for detecting protein interactions such as yeast two-hybrid approach and mass spectrometry to produce vast amounts of data that can be utilized to decipher protein functions in complicated biological networks. In this review, we discuss recent developments in analytical methods for large-scale protein interactions and the future direction of interaction proteomics.

• 농약과학회지
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• 제7권1호
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• pp.1-11
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• 2003

새로운 농약을 탐색하고 개발하는데 있어서 고효율 유기함성 (HTOS) 기술과 고효율 검색 (HTS) 기술 등의 발전과 더불어 컴퓨터 화학을 이용한 분자설계 (CAMD) 방법으로 보편화되고 있는 비교 분자장 분석(CoMFA)과 비교 분자 유사성 지수분석(CoMSIA) 등, 3D QSAR 기법들을 위시하여 분자 홀로그램 구조 - 활성관계 (HQSAR) 분석방법 등, QSAR 기법들을 요약하고 그 활용 사례들을 간략하게 소개하였다.

• Journal of Communications and Networks
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• 제8권2호
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• pp.220-227
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• 2006

In this paper, we present a novel approach to analyze the throughput of direct-sequence spread spectrum multiple access (DS/SSMA) unslotted ALOHA system. In the unslotted system, the departure rate of interfering transmissions is proportional to the number of current interferers that can be regarded as the system state. In order to model this state-dependency, we introduce a two-dimensional state transition model that describes the state transition of the system. This model provides a more rigorous analysis tool for the DS/SSMA unslotted ALOHA systems with both fixed and variable packet lengths. Numerical results reveal that this analysis yields an accurate system performance that coincides with the simulation results. Throughout the analysis we have discovered that the state-dependency of the departure rate causes interference averaging effect in the unslotted system and that this effect yields a higher throughput for the unslotted system than for the slotted system when supported by a strong channel coding.

• Journal of Pharmaceutical Investigation
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• 제30권3호
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• pp.191-199
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• 2000

Genomics is providing targets faster than we can validate them and combinatorial chemistry is providing new chemical entities faster than we can screen them. Historically, the drug discovery cascade has been established as a sequential process initiated with a potency screening against a selected biological target. In this sequential process, pharmacokinetics was often regarded as a low-throughput activity. Typically, limited pharmacokinetics studies would be conducted prior to acceptance of a compound for safety evaluation and, as a result, compounds often failed to reach a clinical testing due to unfavorable pharmacokinetic characteristics. A new paradigm in drug discovery has emerged in which the entire sample collection is rapidly screened using robotized high-throughput assays at the outset of the program. Higher-throughput pharmacokinetics (HTPK) is being achieved through introduction of new techniques, including automation for sample preparation and new experimental approaches. A number of in vitro and in vivo methods are being developed for the HTPK. In vitro studies, in which many cell lines are used to screen absorption and metabolism, are generally faster than in vivo screening, and, in this sense, in vitro screening is often considered as a real HTPK. Despite the elegance of the in vitro models, however, in vivo screenings are always essential for the final confirmation. Among these in vivo methods, cassette dosing technique, is believed the methods that is applicable in the screening of pharmacokinetics of many compounds at a time. The widespread use of liquid chromatography (LC) interfaced to mass spectrometry (MS) or tandem mass spectrometry (MS/MS) allowed the feasibility of the cassette dosing technique. Another approach to increase the throughput of in vivo screening of pharmacokinetics is to reduce the number of sample analysis. Two common approaches are used for this purpose. First, samples from identical study designs but that contain different drug candidate can be pooled to produce single set of samples, thus, reducing sample to be analyzed. Second, for a single test compound, serial plasma samples can be pooled to produce a single composite sample for analysis. In this review, we validated the issue whether the second method can be applied to practical screening of in vivo pharmacokinetics using data from seven of our previous bioequivalence studies. For a given drug, equally spaced serial plasma samples were pooled to achieve a 'Pooled Concentration' for the drug. An area under the plasma drug concentration-time curve (AUC) was then calculated theoretically using the pooled concentration and the predicted AUC value was statistically compared with the traditionally calculated AUC value. The comparison revealed that the sample pooling method generated reasonably accurate AUC values when compared with those obtained by the traditional approach. It is especially noteworthy that the accuracy was obtained by the analysis of only one sample instead of analyses of a number of samples that necessitates a significant man-power and time. Thus, we propose the sample pooling method as an alternative to in vivo pharmacokinetic approach in the selection potential lead(s) from combinatorial libraries.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제8권8호
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• pp.2607-2625
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• 2014

Previous studies on multiuser multiple-input multiple-output (MIMO) mostly assume a homogeneous signal-to-noise ratio (SNR), where each user has the same average SNR. However, real networks are more likely to feature heterogeneous SNRs (a random-valued average SNR). Motivated by this fact, we analyze a multiuser MIMO downlink with a zero-forcing (ZF) receiver in a heterogeneous SNR environment. A transmitter with Mantennas constructs M orthonormal beams and performs the SNR-based proportional fairness (S-PF) scheduling where data are transmitted to users each with the highest ratio of the SNR to the average SNR per beam. We develop a new analytical expression for the sum throughput of the multiuser MIMO system. Furthermore, simply modifying the expression provides the sum throughput for important special cases such as homogeneous SNR, max-rate scheduling, or high SNR. From the analysis, we obtain new insights (lemmas): i) S-PF scheduling maximizes the sum throughput in the homogeneous SNR and ii) under high SNR and a large number of users, S-PF scheduling yields the same multiuser diversity for both heterogeneous SNRs and homogeneous SNRs. Numerical simulation shows the interesting result that the sum throughput is not always proportional to M for a small number of users.

• 한국통신학회논문지
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• 제23권5호
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• pp.1196-1207
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• 1998

본 논문에서는 무선 가입자망(WLL: Wireless Local Loop)을 통하여 데이터 서비스를 제공하는 경우에 있어 Link 부계층의 성능을 연구하고자 한다. Link 부계층에서는 사용자 패킷 데이터를 전송하기에 알맞게 분할하여 전송하게 된다. 데이터를 어느 정도 크기로 분할하느냐에 따라서 패킷 오류 확률이 달라지고 이에 따라서 링크 처리율이 변화하게 된다. 또한 한 패킷 내에서 헤더를 제외한 순수 사용자 데이터가 차지하는 비율이 달라짐에 따라 처리율이 변화하게 된다. 본 논문에서는 WLL 시스템의 재전송 방식을 수학적으로 분석하고 모의 실험함으로써 페이로드의 크기 변화에 따라서 Link 부계층에서의 처리율이 어떻게 변화하는지를 보인다.

• 한국통신학회논문지
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• 제32권9B호
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• pp.589-597
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• 2007

In this paper, a WDM metro ring consisting of access nodes with $FT-FR^n$ (Fixed Transmitter - n Fixed Receivers) is considered. A trade-off exists between node throughput and transmission fairness because the access nodes share wavelength channels. In order to eliminate the transmission unfairness and to increase throughput, the p-persistent medium access control (MAC) protocol is proposed: each node uses an empty optical slot to transmit a packet and make it available with the extraction of a transferred packet at the source access node, called source-stripping. The local empty slot can be used to transfer a head-of-line packet in the local buffer with probability p or it is used for the next downstream nodes with 1-p. The proposed MAC protocol provides better node throughput than the non-persistent protocol and exhibits better fairness index than the 1-persistent protocol in WDM ring networks. In addition, numerical analysis shows that the proposed MAC protocol maximizes the node throughput under uniform traffic conditions. For more detailed results, we use the network simulation under Poisson and self-similar traffic. Furthermore, unpredictable traffic constructed by the combination of the former and the latter is also considered. The reasonable probability of the p-persistent protocol for a given architecture can be determined through simulation.