• Title/Summary/Keyword: threshold concentration

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Studies on the Evaluation for the Quality of Food by Sensory Testing -I. Selection of Sensory Panel and Difference Testing for the Evaluation on the Sensory Quality of Milk- (관능검사법(官能檢査法)에 의한 식품(食品)의 품질(品質) 평가(評價)에 관(關)한 연구(硏究) -제(第) 1 보(報) : 우유의 관능(官能) 품질(品質)의 평가(平價)를 위한 파넬원 선발(選拔) 및 차이(差異) 식별(識別) 시험(試驗)-)

  • Chae, Soo-Kyu;Chang, Kun-Hyung
    • Korean Journal of Food Science and Technology
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    • v.12 no.3
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    • pp.150-157
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    • 1980
  • In an attempt to evaluate the quality of food by sensory testing, the difference testing for commercial milk was conducted by selected members of a sensory panel. The results were summarized as follows: 1. Sensory panel of 40 persons were selected by the general basis of selection of panel members, by sensitivity test for primary taste, and by discriminatory ability test and trained to have normal abilities on the sensory testing for the quality of milk. 2. As a result of sensitivity test for 4 primary tastes by panel members, the solution concentration of salt, sour, bitter and sweet for which average recognition threshold was located were 0.0128 M sodium chloride, 0.0008 M citric acid, 0.0016 M caffeine and 0.0256 M sucrose, respectively. 3. As results of difference test of single stimuli, paired comparison test, duo-trio test and triangle test, a significant difference was recognized at higher level than the 1% among the samples of commercial milk produced by 5 companies.

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Determination of geosmin and 2-MIB in Nakdong River using headspace solid phase microextraction and GC-MS (HS-SPME-GC/MS를 이용한 낙동강 수계 하천수 중 조류기원성 냄새물질 분석)

  • Lee, Injung;Lee, Kyoung-Lak;Lim, Tae-Hyo;Park, Jeong-Ja;Cheon, Seuk
    • Analytical Science and Technology
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    • v.26 no.5
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    • pp.326-332
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    • 2013
  • Geosmin and 2-methylisoborneol (2-MIB) are volatile organic compounds responsible for the majority of unpleasant taste and odor events in drinking water. Geosmin and 2-MIB are byproducts of blue-green algae (cyanobacteria) with musty and earthy odors. These compounds have odor threshold concentration at ng/L levels. It is needed to develop a sensitive method for determination of geosmin and 2-MIB to control the quality of drinking water. In this study, geosmin and 2-MIB in water samples were determined by gas chromatography-mass spectrometry (GC-MS) with headspace-solid phase microextraction (HS-SMPE). The detection limits of this method were 1.072 ng/L and 1.021 ng/L for geosmin and 2-MIB, respectively. Good accuracy and precision was also obtained by this method. Concentrations of the two compounds were measured in raw waters from Nakdong River in the cyanobacterial blooming season. Water bloom formed by cyanobacteria has been occurred currently in Nakdong River. It is needed to investigate the concentrations of geosmin and 2-MIB to control the quality of drinking water from Nakdong River. Both geosmin and 2-MIB were detected in raw waters from Nakdong River at concentrations ranging from 4 to 24 ng/L and 6 to 16 ng/L, respectively.

Anisomycin, an Inhibitor of Protein Synthesis, Overcomes TRAIL Resistance in Human Hepatocarcinoma Cells via Caspases Activation and Bid Downregulation (Caspase 활성 및 Bid의 발현 저하를 통한 단백질 생성 억제제인 anisomycin의 인체간암세포에서 TRAIL 매개 apoptosis 유발의 활성화)

  • Jin, Cheng-Yun;Park, Cheol;Hong, Su Hyun;Choi, Yung Hyun
    • Journal of Life Science
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    • v.24 no.7
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    • pp.769-776
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    • 2014
  • Anisomycin, also known as flagecidin, is an antibiotic produced by Streptomyces griseolus that inhibits protein synthesis by binding to the ribosomal 28S subunit. The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a protein that induces apoptotic cell death. TRAIL primarily causes apoptosis in tumor cells by binding to death receptors. Many human cancer cell lines are refractory to TRAIL-induced cell death. In this study, we investigated whether anisomycin could enhance TRAIL-mediated apoptosis in TRAIL-resistant human hepatocarcinoma Hep3B cells. Treatment with anisomycin and TRAIL alone did not reduce cell viability in Hep3B cells. However, in the presence of TRAIL, the anisomycin concentration dependently reduced the cell viability. Our results indicate that anisomycin sensitizes Hep3B cells to TRAIL-mediated apoptosis and that this occurs, at least partly, via caspase activation. Interestingly, Bid knockdown by small interfering RNA significantly reduced the induction of apoptosis in combination with anisomycin and TRAIL, indicating that anisomycin effectively acts to lower the threshold at which TRAIL-mediated truncated Bid triggers the mitochondrial-mediated apoptosis program in Hep3B cells. Therefore, the use of TRAIL in combination with anisomycin might provide an effective therapeutic strategy for the safe treatment of some TRAIL-resistant cancer cells.

Simulation of Plume Length Induced by Orimulsion Combustion (오리멀젼 연소시 발생하는 백연의 연기거리 전산모사)

  • Kwak, Byoung-Kyu;Kim, Jong-Ho;Joo, Ji-Bong;Lee, Jeong-Jin;Kim, Jin-Soo;Kim, Young-Hun;Yi, Jong-Heop
    • Clean Technology
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    • v.14 no.2
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    • pp.136-143
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    • 2008
  • The objective of this study is to investigate the visibility of plume at the Y power plant stack, which fires the orimulsion as a fuel. The plume contains numerous primary particles under $1\;{\mu}m$ size and inorganic ions possibly inferred by the chemicals of secondary aerosol formation. We evaluated the visibility of the plume using the modified PLUVUE-II model. The monitoring data on the particle size distribution (PSD) and secondary aerosols of sulfate were applied to estimate and evaluate the main factors of plume opacity. The chemical reactions were applied to the model for the secondary aerosol formation of $(NH_4)_2SO_4(s)$. The maximum plume length was estimated by an optic method using threshold contrast. The results showed that the plume length was strongly dependent upon the PSD and $(NH_4)_2SO_4(s)$ concentration of the plume emitted from the stack.

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Removal Characteristics of Natural Organic Matter and Taste and Odor by Advanced Water Treatment Process around the Han River Water Supply System (한강수계 고도정수처리 공정에서의 유기물과 맛·냄새의 제거특성)

  • Jae-Lim Lim;Lee, Kyung-Hyuk;Kim, Seong-Su;Chae, Seon-Ha
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.1
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    • pp.13-25
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    • 2007
  • The water treatment plants in Seoul Metropolitan Area, which are under Korea Water Resources Corporation(KOWACO)'s management, take water from Paldang Reservoir in Han River System for drinking water supply. There are taste and odor (T&O) problems in the finished water because the conventional treatment processes do not effectively remove the T&O compounds. As part of countermeasures for taste and odor control, KOWACO is planning to introduce advanced water treatment process such as ozone and GAC in near future. This study evaluated the removal characteristics of T&O and dissolved organic matter (DOM) to find design and operation parameters of advanced water treatment processes in a pilot-scale treatment plant. The GAC adsorption capacity for DOC in the two GAC system (GAC and $O_3$-GAC) at an EBCT of 14min was mostly exhausted after 9months. The differency of the removal efficiency of DOC between $O_3$-GAC and GAC increased with increasing operation time because the bioactivity in $O_3$-GAC process was enhanced by post-ozone process. Removal by conventional treatment was unable to reach the target TON(threshold odor number) of 3 but GAC systems at an EBCT(empty bed contact time) of 14 min were able to archive the target with few exception. During the high T&O episodes, PAC as a pretreatment together with GAC could be useful option for T&O control. However, substantial TON removal continued for more than two year (> 90,000 bed volumes). At the spiking of less concentration 26 to 61 ng/L in the influent of GAC systems, GAC absorber and $O_3$-GAC processes could meet the treatment target. The better spike control after 12 and 19 months of operation compared to that after 7 months of operation is a strong indication of biological control. The results presented in this study had shown that $O_3$-GAC process was found to be more effective for T&O control than GAC process. And the main removal mechanism in GAC systems were adsorption capacity and biodegradation.

VOC/HAPs Emission Characteristics & Adsorption Evaluation for Paint Products in Busan Area (부산지역 페인트제조시설의 VOC/HAPs 배출특성 및 흡착능 평가)

  • Song, Bok-Joo;Lee, Seung-Min;Cho, Gab-Je;Cho, Jeong-Gu;You, Pyung-Jong;Kim, Gi-Gon
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.5
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    • pp.316-325
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    • 2012
  • In this study, we suggested adequate control technology by analyzing emission process and main chemical of VOC/HAPs generated from four paint factories in Busan area. And we estimated whether Kapok fiber, which is a natural material, can be commercially used for an absorbent by testing adsorption ability. As a result of this sturdy, above 60% of VOC/HAPs was volatilized and dispersed inside the working place without conducting control system of facilities during manufacturing paints. Concentration profile of VOC/HAPs, which is volatile naturally outside the factories, is surveyed above 70% at Toluene, Ethylbenzene, and Xylene. And a result of evaluation of odor attribution level about the component whose Odor Threshold is known, it is estimated that major cause material of A, B, C factories is Toluene and that of D factory is m/p-Xylene. And that result presented design arguments such as facilities specifications, activated carbon filling volume, and replace cycle of activated carbon as control technology. Also, that result presented emission process improvement such as adsorption of central-controlled ventilation device, installation of inlet flenge, and potable cleaning process. The rate of pollutant adsorption of Kapok fiber, which is natural material, is indicated about 91.9%, 66.7%. That result validated the possibility as replacement of activated carbon.

Purification of a Bradykinin-Related Peptide from the Skin of Hagfish. Eptatretus burgeri (먹장어 (Eptatretus burgeri)의 피부로부터 Bradykinin-Related Peptide의 정제)

  • SHIN Mi Jung;KIM Eun Jung;KIM Chan-Hee;GO Hye-Jin;KIM In Hae;RYU Hong-Soo;Huh Min-Do;CHUNG Joon-Ki;PARK Nam Gyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.1
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    • pp.30-34
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    • 2003
  • A hagfish bradykinin(BK)-related peptide was isolated and characterized from the skin of hagfish, Eptatretus burgeri. The hagfish BK with a molecular mass of 875.8 Da was purified to a homogeneity using $C_{18}$ reverse-phase and cation-exchange high performance liquid chromatography. The primary structure of the hagfish BK was determined as Gly-Thr-Ala-Gly-Ile-Gly-Pro-Phe-Arg by a combination of an automated amino acid sequencing and MALDI-TOF mass spectrometry. This amino acid sequence contains five substitutions $(Arg^1{\rightarrow}Gly,\;Pro^2{\rightarrow}Thr,\;Pro^3{\rightarrow}Ala,\;Phe^5{\rightarrow}Ile,\;Ser^6{\rightarrow}Gly)$ compared with that of mammalian BK. The hagfish BK showed a contractile action on the intestine of hagfish, Eptatretus burgeri. The threshold concentration of hagfish BK was around $10^{-11}\;M.$

Chemical Characteristics of Groundwater in Carbonate Rock Areas of Korea

  • Kabir, Mohammad Lutful;Park, Youngyun;Lee, Jin-Yong
    • Journal of Soil and Groundwater Environment
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    • v.19 no.2
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    • pp.7-15
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    • 2014
  • This study was conducted to understand the chemical characteristics of groundwater in carbonate areas of Korea. In this study, data on pH, electric conductivity (EC), $Ca^{2+}$, $Mg^{2+}$, $Na^+$, $K^+$, $Cl^-$, $SO_4{^{2-}}$, and $HCO_3{^-}$ were collected from 97 wells which were installed in various carbonate rock regions of Korea. The pH values ranged from 5.7 to 9.9, and the average value was 7.3. The concentration range showed differences between the maximum value of $HCO_3{^-}$ and the medium to minimum values of $Ca^{2+}$, $Mg^{2+}$, $Na^+$, $Cl^-$, $SO_4{^{2-}}$, and $K^+$ in the study area. The average value of EC was $374{\mu}S/cm$, higher than in granite and gneiss areas, where the value is $176{\mu}S/cm$. Most of the groundwater was type $Ca-HCO_3$, and some was type $Mg-HCO_3$. The relationship between $Ca^{2+}$, $Cl^-$, and $HCO_3{^-}$, respectively, and EC showed relatively significant positive correlations compared to the other dissolved components. However, the determination coefficients for $Mg^{2+}$, $Na^+$, $SO_4{^{2-}}$, and $K^+$ were very low less than 0.2. These results indicate that the source of $Ca^{2+}$ and $Mg^{2+}$ is relatively simple (carbonate dissolution) compared to other sources. The sources of $Na^+$, $K^+$, $Cl^-$, $SO_4{^{2-}}$, and $HCO_3{^-}$ might be not only water-rock interactions, but also irrigation return flow, because many groundwater wells had been developed for irrigation purposes. Subsequently, the influence of agriculture on groundwater chemistry was evaluated using a cumulative plot of $SO_4{^{2-}}$. The threshold value of $SO_4{^{2-}}$ calculated from the cumulative frequency curve was 29.2 mg/L. Therefore, 12.4% of all the groundwater wells were affected by agricultural activity.

A Harmonized Method for Dose-response Risk Assessment Based on the Hazard & Risk Evaluation of Chemicals (HREC) According to the Industrial Safety and Health Act (ISHA) (산업안전보건법 상 유해성.위험성 평가제도 적용을 위한 양-반응 평가의 통일화 방안 연구)

  • Lim, Cheol-Hong;Yang, Jeong-Sun;Park, Sang-Yong
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.22 no.3
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    • pp.175-183
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    • 2012
  • Objectives: This study developed a harmonized method for risk assessment based on the Hazard & Risk Evaluation of Chemicals (HREC) according to the Industrial Safety and Health Act (ISHA). Methods: Three preliminary studies, performed during 2010 and 2011 by the Occupational Safety and Health Research Institute and three academic research groups, were compared. The differences in risk assessment, especially in the dose-response assessment method, were analyzed. A new harmonized method for dose-response assessment was suggested and its applicability for the HREC was examined. Results: Considering the various steps of each dose-response assessment, the equivalent steps in quantitative correction, uncertainty factor 2 (UF2) for intra-species uncertainty, and UF3 for the experimental period in the uncertainty correction were relatively high. Using our new method, the total correction values (quantitative correction plus uncertainty correction) ranged from 72~15,789 to 30~60, and the ratio of the threshold limit value (TLV) to the reference concentration decreased from 12.8~1900 to 5.4~11.8. Furthermore, when we performed risk characterization by our new method, hazard quotient (HQ) values for chloroethylene, epichlorohydrin, and barium sulfate became 3.0, 14.1, and 1.13 respectively, whereas three previous studies reported HQ values of 7.1, 4580, and 87.3 considering reasonable maximum exposure (RME) conditions. HQs of the three chemicals were calculated to be 0.6, 2.4, and 0.1 respectively, when compared to their TLVs. Conclusions: Our new method could be applicable for the HREC because the total correction values and the ratio of TLVs were within reasonable ranges. It is also recommended that additional risk management measures be applied for epichlorohydrin, for which the HQ values were greater than 1 when compared with both reference values and the TLV. Our proposed method could be used to harmonize dose-response assessment methods for the implementation of risk assessment based on the HREC according to ISHA.

A study on exposure of organic solvents in manufacturing industry (제조업 산업장의 유기용제 노출에 관한 연구)

  • Moon, Deog-Hwan;Kim, Jeong-Ha;Kim, Pil-Ja;Park, Myung-Hee;Hwang, Yong-sik;Lee, Chae-Kwan;Lee, Chang-Hee
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.11 no.3
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    • pp.219-228
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    • 2001
  • Objective : For the purpose of preparing the fundamental data and health promotion and control program on organic solvents in air of manufacturing industry. Methods : The author surveyed number of organic solvent components which was used in working site and also determined the organic solvents concentration in air of 927 manufacturing industries and 1,267 working process with gas chromatography(NIOSH manual) for five years from 1995 to 1999. Results : Mean number of solvents components by type of industry, working process was number of 12. There were exceeded to TLV of 1,2-dichloroethane in textile manufacturing industry N,N-dimethyl furan in tanning and dressing of leather ; luggage, handbags, saddlery, harness and footwear manufacturing industry and chemical and chemical product manufacturing industry by type of industry. There were exceeded to TLV of 1,2-dichloroethane in handwriting and drawing process, cellosolve in adhesive spreading process, N,N-dimethly furan in production of solvent process and adhesion process by working process Total exceeded rate to threshold limit values of organic solvents mixture were 12.9% for EI(Exposure index) and 10.0% for Em(Estimation of mixture) by type of industry, 11.3% for EI and 8.2% for Em by type of working process. The highest exceed rate was 36.7% for EI in tanning and dressing of leather ; luggage handbags, saddlery, harness and footwear manufacturing industry and 29.0% for Em in textile manufacturing industry. The highest exceeded rate was 23.1% for EI and 12.5% for Em in adhesive spreading process by working process. Mean values of total subjects by type of industry and type of working process were $0.7{\pm}1.8$ for EI and $0.7{\pm}1.7$ for Em respectively. Conclusions : As above results, the author suggest that it makes the environmental control program on 1.2-dichloroethane, N,N-dimethyl furan, cellosolve by kind of organic solvent and on textile manufacturing industry, tanning and dressing of leather ; luggage, handbags, saddlery harness and footwear manufacturing industry by type of industry, and on handwriting, drawing process and adhesive spreading process and adhesion process by type of working process.

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