• BMB Reports
• /
• v.28 no.6
• /
• pp.477-483
• /
• 1995

The recombinant human interferon alpha 2a ($rhIFN-{\alpha}2a$), expressed in Saccharomyces cerevtsiae, was purified from insoluble aggregates. The inclusion body of $rhIFN-{\alpha}$ was solubilized by guanidine salt in the presence of disulfide reducing agent. The refolding of denatured $rhIFN-{\alpha}2a$ was achieved by simple dilution. The authentic interferon alpha, which has two correctly matched disulfide bonds, was seperated from incompletely oxidized $IFN-{\alpha}$ and dimeric $IFN-{\alpha}$ by use of a CM-Sepharose column, followed by size exclusion columns at two different pH conditions. The purified protein has been subjected to detailed physicochemical characterization including sequence determination. Unlike other $rhIFN-{\alpha}2a$ from E. coli reported, the $rhIFN-{\alpha}2a$ from S. cerevisiae has no methionine residue at its N-terminus originating from the start codon, ATG. The pI of the protein was determined to be 6.05 with a single band in the pI gel, which demonstrated that the purified $rhIFN-{\alpha}$ was homogeneous. The structural study using circular dichroism showed that the protein retains its three dimensional structure in the wide range of pH conditions between pH 3 and 9, and only minor strucural deformation was observed at pH 1.0.

• The korean journal of orthodontics
• /
• v.52 no.2
• /
• pp.85-101
• /
• 2022

Objective: To classify facial asymmetry (FA) phenotypes in adult patients with skeletal Class III (C-III) malocclusion. Methods: A total of 120 C-III patients who underwent orthognathic surgery (OGS) and whose three-dimensional computed tomography images were taken one month prior to OGS were evaluated. Thirty hard tissue landmarks were identified. After measurement of 22 variables, including cant (°, mm), shift (mm), and yaw (°) of the maxilla, maxillary dentition (Max-dent), mandibular dentition, mandible, and mandibular border (Man-border) and differences in the frontal ramus angle (FRA, °) and ramus height (RH, mm), K-means cluster analysis was conducted using three variables (cant in the Max-dent [mm] and shift [mm] and yaw [°] in the Manborder). Statistical analyses were conducted to characterize the differences in the FA variables among the clusters. Results: The FA phenotypes were classified into five types: 1) non-asymmetry type (35.8%); 2) maxillary-cant type (14.2%; severe cant of the Max-dent, mild shift of the Man-border); 3) mandibular-shift and yaw type (16.7%; moderate shift and yaw of the Man-border, mild RH-difference); 4) complex type (9.2%; severe cant of the Max-dent, moderate cant, severe shift, and severe yaw of the Man-border, moderate differences in FRA and RH); and 5) maxillary reverse-cant type (24.2%; reverse-cant of the Max-dent). Strategic decompensation by pre-surgical orthodontic treatment and considerations for OGS planning were proposed according to the FA phenotypes. Conclusions: This FA phenotype classification may be an effective tool for differential diagnosis and surgical planning for Class III patients with FA.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.5 no.9
• /
• pp.1632-1652
• /
• 2011

This paper considers the object tracking problem in three dimensional (3-D) space when the azimuth and elevation of the object are available from the passive acoustic sensor. The particle filtering technique can be directly applied to estimate the 3-D object location, but we propose to decompose the 3-D particle filter into the three planes' particle filters, which are individually designed for the 2-D bearings-only tracking problems. 2-D bearing information is derived from the azimuth and elevation of the object to be used for the 2-D particle filter. Two estimates of three planes' particle filters are selected based on the characterization of the acoustic sensor operation in a noisy environment. The Cramer-Rao Lower Bound of the proposed 2-D particle filter-based algorithm is derived and compared against the algorithm that is based on the direct 3-D particle filter.

• Journal of Microbiology and Biotechnology
• /
• v.16 no.9
• /
• pp.1453-1458
• /
• 2006

PKR-like endoplasmic reticulum (ER) kinase (PERK) is a type I transmembrane ER-resident protein containing a cytoplasmic catalytic domain with a Ser/Thr kinase activity, which is most closely related to the eukaryotic translation initiation factor-$2{\alpha}$ ($eIF2{\alpha}$) kinase PKR involved in the antiviral defense pathway by interferon. We cloned and expressed the PERK C-terminal kinase domain (cPERK) in Escherichia coli. Like PERK activation in cells under ER stress, wild-type cPERK underwent autophosphorylation when overexpressed in E. coli, whereas the cPERK(K621M) with a methionine substitution for the lysine at amino acid 621 lost the autophosphorylation activity. The activated form cPERK which was purified to near homogeneity, formed an oligomer and was able to trans-phosphorylate specifically its cellular substrate $eIF2{\alpha}$. Two-dimensional phosphoamino acids analysis revealed that phosphorylation of cPERK occurs at the Ser and Thr residues. The functionally active recombinant cPERK, and its inactive mutant should be useful for the analysis of biochemical functions of PERK and for the determination of their three-dimensional structures.

• Nuclear Engineering and Technology
• /
• v.53 no.9
• /
• pp.2968-2976
• /
• 2021

This paper introduces a method to reconstruct the three-dimensional (3D) microstructure of two-phase materials, e.g., porous materials such as highly irradiated nuclear fuel, from two-dimensional (2D) sections via a multi-objective optimization genetic algorithm. The optimization is based on the comparison between the reference and reconstructed 2D sections on specific target properties, i.e., 2D pore number, and mean value and standard deviation of the pore-size distribution. This represents a multi-objective fitness function subject to weaker hypotheses compared to state-of-the-art methods based on n-points correlations, allowing for a broader range of application. The effectiveness of the proposed method is demonstrated on synthetic data and compared with state-of-the-art methods adopting a fitness based on 2D correlations. The method here developed can be used as a cost-effective tool to reconstruct the pore structure in highly irradiated materials using 2D experimental data.

• The Bulletin of The Korean Astronomical Society
• /
• v.41 no.1
• /
• pp.54.2-54.2
• /
• 2016

Korea Microlensing Telescope Network (KMTNet) is the first optical survey system of its kind in a way that three KMTNet observatories are longitudinally well-separated, and thus have the benefit of 24-hour continuous monitoring of the southern sky. The wide-field and round-the-clock operation capabilities of this network facility are ideal for survey and the physical characterization of small Solar System bodies. We obtain their orbits, absolute magnitudes (H), three dimensional shape models, spin periods and spin states, activity levels based on the time-series broadband photometry. Their approximate surface mineralogy is also identified using colors and band slopes. The automated observation scheduler, the data pipeline, the dedicated computing facility, related research activity and the team members are collectively called 'DEEP-South' (DEep Ecliptic Patrol of Southern sky). DEEP-South observation is being made during the off-season for exoplanet search, yet part of the telescope time is shared in the period between when the Galactic bulge rises early in the morning and sets early in the evening. We present here the observation mode, strategy, software, test runs, early results, and the future plan of DEEP-South.

• Korean Journal of Soil Science and Fertilizer
• /
• v.48 no.4
• /
• pp.285-294
• /
• 2015

Ordination has been recognized useful method to analyze the effects of multiple environmental factors on dozens of species in vegetation ecology because of summarizing community data by producing a low-dimensional graphics. Main objective of this study was the application of ordination method, especially principal components analysis (PCA), to analyze the soil characterization on paddy treated by different green manure crops and tillage methods. Treatments included the three tillage treatments and two green manure crops as the following; (i) moldrotary + rotary tillage without green manure crop (Con), with (ii) hairy vetch (ConHv), and (iii) hairy vetch + green barely (ConHvGb), (iv) rotary tillage without green manure crop (Rot), with (v) hairy vetch (RotHv), and (vi) hairy vetch + green barly (RotHvGb), and (vii) no-tillage (Notill). Vectorial distance result from PCA of soil properties including physical, chemical, and microbial properties showed the two main difference. Firstly, soil properties among plots without green manure were strongly affected by tillage strength [Vectorial distance: Con-Notil (5.88) > Rot-Notill (4.58)] at PC1 (35.0%) axis. But it was difficult to find the fixed trend among plots when green manure crop was added in plot. Nevertheless, two groups were separated by adding green manure crop at PC2 (29.2%) axis. These results show that PCA ordination methods could be used the research for change of soil characterization.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.18 no.3
• /
• pp.729-737
• /
• 1994

A three-dimensional finite element model of a 20-pin plastic dual-in-line package(PDIP) plugged into a PCE has been developed by using the finite element code ANSYS. The model has been used for thermal characterization of the package during its normal operation under natural convection cooling. Temperature distributions in the package and PCB are obtained from numerical analysis and compared with experimentally measured data. Various cases are assumed and analyzed to study the effects of package and PCB materials on thermal characteristics of PDIP with and without aluminum heatspreader. Thermal dissipation capability of PDIP is greatly increased due to copper die pad/lead frame and heatspreader. However, thermally induced stresses in the package and fatigue life of chip are improved for PDIP with Alloy 42 die pad/lead frame and no heatspreader. It is also found that the role of PCB on thermal characteristics of PDIP is very imporatant.

• Proceedings of the Korean Society For Composite Materials Conference
• /
• 2002.05a
• /
• pp.57-60
• /
• 2002

The stiffness model has been proposed to predict elastic constants of twisted yarn composites. The model is based upon the unit cell structure, the coordinate transformation, and the volume averaging of compliance constants for constituent materials. For the correlation of analytic results with experiments, composite samples of various yarn twist angle were tested. The samples were fabricated by the RTM process using glass yarns and epoxy resin. The correlations of elastic constants showed relatively good agreements. The model provides the predictions of the three-dimensional engineering constants, which are valuable input data for the analytic characterization of textile composites made of twisted yarn.

• Proceedings of the Korean Society For Composite Materials Conference
• /
• 2001.10a
• /
• pp.64-69
• /
• 2001

In general, laminate effective orthotropic thermal conductivities are dependent on fiber and matrix material properties, fiber volume fraction and fabric geometric parameters. This paper deals with the predicting method of the transverse and the in-plane thermal conductivities of plain weave fabric composites based on the three dimensional series-parallel thermal resistance network. Thermal resistance network was applied to unit cell model that characterizes the periodically repeated pattern of plain weave. Also, an experiment apparatus is setup to measure the thermal conductivities of composite material. The numerical and experimental results of carbon/epoxy plain weave are compared.