• Title/Summary/Keyword: three-dimension culture

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Effects of Culture Dimensions on Maintenance of Porcine Inner Cell Mass-Derived Cell Self-Renewal

  • Baek, Song;Han, Na Rae;Yun, Jung Im;Hwang, Jae Yeon;Kim, Minseok;Park, Choon Keun;Lee, Eunsong;Lee, Seung Tae
    • Molecules and Cells
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    • v.40 no.2
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    • pp.117-122
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    • 2017
  • Despite the fact that porcine embryonic stem cells (ESCs) are a practical study tool, in vitro long-term maintenance of these cells is difficult in a two-dimensional (2D) microenvironment using cellular niche or extracellular matrix proteins. However, a three-dimensional (3D) microenvironment, similar to that enclosing the inner cell mass of the blastocyst, may improve in vitro maintenance of self-renewal. Accordingly, as a first step toward constructing a 3D microenvironment optimized to maintain porcine ESC self-renewal, we investigated different culture dimensions for porcine ICM-derived cells to enhance the maintenance of self-renewal. Porcine ICM-derived cells were cultured in agarose-based 3D hydrogel with self-renewal-friendly mechanics and in 2D culture plates with or without feeder cells. Subsequently, the effects of the 3D microenvironment on maintenance of self-renewal were identified by analyzing colony formation and morphology, alkaline phosphatase (AP) activity, and transcriptional and translational regulation of self-renewal-related genes. The 3D microenvironment using a 1.5% (w/v) agarose-based 3D hydrogel resulted in significantly more colonies with stereoscopic morphology, significantly improved AP activity, and increased protein expression of self-renewal-related genes compared to those in the 2D microenvironment. These results demonstrate that self-renewal of porcine ICM-derived cells can be maintained more effectively in a 3D microenvironment than in a 2D microenvironment. These results will help develop novel culture systems for ICM-derived cells derived from diverse species, which will contribute to stimulating basic and applicable studies related to ESCs.

Development of Dress Forms for the Aged Women Based on Their Body Shapes Applying 3D Body Scan Data (3차원 인체 형상을 이용한 실버 여성 패션 산업용 인대 모형 개발)

  • Kim, Soo-A;Choi, Hei-Sun
    • The Research Journal of the Costume Culture
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    • v.18 no.1
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    • pp.80-92
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    • 2010
  • This research aims at developing the dress form for the aged women based on their body shapes using the three-dimensional body scan data with the body shape categorization(according to the previous research). To accomplish this goal, the sample group of representative body shape of the 50% of median was selected by using the high frequency proportion range of each type of body shape of the aged women, and the sample group of representative body shape of each type was averaged in a three-dimensional way by using the morphing method of a three-dimension reverse-engineered software. RP in the form of torso was produced based on the shape data of the final model and the data was formed into an actual object, by which an aged women's dress form model was drawn out. The differences of the girth of the bust, hip and waist between the developed dress form model and the existing dress form model were examined. The result showed that the developed dress form had a bigger size of waist girth than that of bust and hip girth, compared to the existing dress form, which shows that it reflects the aged women's tendency of abdomen obesity, so it's expected to be more proper for the human bodies of the targeted age group than the existing dress form. These research results may help design the clothing suitable for the body shape of the aged women so that their demand for the clothing of good fit will be satisfied in the future.

A Transcultural Reflection on Anglo-Chinese Gardens in the 18th Century (18세기 '중국풍 정원(Anglo-Chinese garden)'의 문화전이에 관하여)

  • Kim, Daesin
    • The Journal of Art Theory & Practice
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    • no.16
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    • pp.201-224
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    • 2013
  • The tradition of the representative art style in the Sinosphere, Shanshui hua, expresses the traditional representation of the harmony and principle of the universe. This tradition is reflected in the Chinese garden. These Chinese gardens were precisely the three-dimension representations of Shanshui hua, a visual form of abstract expression of the oriental philosophical thinking. This research determines and draws attention to the vestiges of the reflection of Shanshui hua in the European gardens through visual art and culture. It will also approach the two subjects, Shanshui hua and garden, from a transcultural view to integrally analyze visual art. The appearance of Anglo-Chinese gardens, reflecting Shanshui hua, foreshowed a big change in traditional European gardens. This is a concrete example of the transcultural phenomenon. This has formed the typical naturally curved English gardens in the gardening history. This also divided these English gardens completely from the symmetrical, geometrical French gardens. This study considers the influence and the reverberation of Shanshui hua reflected on European gardens in the European culture. The cultural exchange of European and Chinese styles in the 18th century left an impact on the European gardening style history. Finally, this study analyzes the origin of these Anglo-Chinese gardens and its content to approach it with a transcultural view as a research methodology.

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Dimensions of Fashion Store Salesperson's Effort and Importance of Effort Dimension (패션점포 판매원의 노력 차원과 중요도 -중.상층 노년여성고객과 판매원 관점을 중심으로-)

  • 신혜봉;임숙자
    • The Research Journal of the Costume Culture
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    • v.11 no.1
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    • pp.103-117
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    • 2003
  • The aim of this study was to identify dimensions of salesperson´s effort according to older female(55+) customer's and fashion store salesperson's perspective and to examine what kind of effort dimensions are assessed to be more important than others. As a method, both literature review and empirical research were made. This study was performed in three stage from December in 2001 to May in 2002. For exploratory step. In-depth interview and observation study were done for gathering responses related to salesperson´s effort. in pilot study, 83 older female customers were surveyed and the data was analyzed in order to develop questionnaire. In the main research. 202 older female customers and 103 fashion store salespersons were surveyed in order to identify the dimensions of salesperson's effort. Statistical analyses were performed with SAS program using factor analysis. cronbach´a mean, t-test. paired t-test and multiple regression analysis. The results of this study were as follows. First, 5 factors was selected for the dimensions of salesperson´s effort in customer´s perception; friendliness, attentiveness. product competence. effective access and special treatment. Second, 5 factors was selected for the dimensions of salesperson's effort in salesperson´s performance; friendliness/ attentiveness. product competence. effective access. communication, and purchase exaction. Third. dimensions of salesperson's effort were different according to customer and salesperson's characteristics. Last, there were significant differences in the salesperson's efforts between customers´perception and salespersons´Performance.

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Brand Images of Children's Wear and Mother's Purchase Intention -Focus on Self-Image Congruence and Behavioral Intention Model- (주부가 선호하는 아동복 브랜드의 이미지에 따른 구매의도 -자기일치성과 행동의도모델을 중심으로-)

  • Kim, Ji-Yeon;Lee, Kyu-Hye
    • The Research Journal of the Costume Culture
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    • v.19 no.3
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    • pp.622-636
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    • 2011
  • The purpose of this study was to assess the effects of self-image congruence on attitudes toward purchase intentions of children's clothing via the Behavioral Intention Model. The empirical study was conducted via on-line survey and data were collected from mothers with children aged 6 to 10 years. A total of 593 respondents answered the questionnaire and 574 usable data were statistically analyzed. SPSS 18.0 was used to conduct descriptive statistical analysis, factor analysis, reliability analysis, cluster analysis, Chi-square test, ANOVA, and multiple regressions. A K-means cluster analysis was conducted based on three dimensions brand images of children's wear. Respondents were divided into four groups: elegant image group, multiple image group, ordinary image group, and childlike image group. Characteristics of consumers and clothing evaluative criteria that mothers considered important differed significantly across groups. Moreover, based on these groups, each dimension of self-congruence had different effects on brand attitude. Brand attitude and subjective norms had different effects on purchase intentions. In conclusion, levels of self-congruence and factors influencing purchase intention varied according to brand images of children's wear.

Repurchase intention toward fast fashion brands - Product characteristics, consumer characteristic, and cognitive dissonance - (패스트 패션 브랜드에 대한 재구매 의도 - 제품 특성, 소비자 특성, 인지부조화 -)

  • Jeon, Kyung Sook;Park, Hye-Jung
    • The Research Journal of the Costume Culture
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    • v.23 no.6
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    • pp.940-954
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    • 2015
  • The purpose of this study is to identify the antecedents of repurchase intention toward fast fashion brands. Perceived quality, perceived price, deindividuation, and overly trendy styles, which are product characteristic variables, and fashion innovativeness, which is a consumer characteristic variable, were considered as antecedents. It was hypothesized that product and consumer characteristics influence repurchase intention toward fast fashion brands not only directly, but also indirectly through cognitive dissonance. Data were gathered by surveying university students in Seoul using convenience sampling. Three hundred and fifty-two questionnaires were used in the statistical analysis. SPSS was used for exploratory factor analysis, and AMOS was used for confirmatory factor analysis and path analysis. The factor analysis of product characteristics revealed four dimensions: "perceived quality", "perceived price", "deindividuation", and "overly trendy styles", and the factor analysis of consumer characteristics revealed one dimension. The factor analysis of cognitive dissonance revealed two dimensions, "regrets" and "perceived uncertainty." The hypothesized path test proved that perceived quality, deindividuation, overly trendy styles, and fashion innovativeness influence repurchase intention directly. Perceived price and deindividuation influence repurchase intention indirectly through the factor of cognitive dissonance, which is the perceived uncertainty, indicating the importance of cognitive dissonance. The results indicate effective marketing strategies should be used to decrease consumers' cognitive dissonance, and suggestions for future study are provided.

Association between Shopping Items and the Demographics of Foreign Tourists in South Korea

  • Jeong, Dong-Bin
    • East Asian Journal of Business Economics (EAJBE)
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    • v.7 no.3
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    • pp.63-73
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    • 2019
  • Purposes - In this research, we look over and investigate associations between shopping items and the demographics of foreign tourists in South Korea. The related seven variables are gender, age, occupations, country of residence, visit month, visit purpose and trip type. In addition, we can graph twenty-one shopping items in association with the demographics of foreign tourists by computing their dissimilarity or similarity on two dimension planes granting that the association exists between the underlying variables. Research design and methodology - This research is performed by Ministry of Culture and Tourism in 2017 and investigated 13,200 foreign tourists from 20 countries. For analyzing the detailed relationships between shopping items and the demographics of foreign tourists, we take advantage of both independent test and correspondence analysis as key statistical techniques. Results - The findings show that shopping items which foreign tourists purchase are closely associated with the three different demographics variables such as country of residence, tour type and visit purpose by monitoring significant p-value of chi-squared statistic Conclusions - This study suggests Ministry of Culture, Sports and Tourism must explore ways toward tourism infrastructure such as global marketing, municipality strategy for attracting foreign tourists, development of diverse shopping items and services and so on.

Analysis of Service Quality Attributes in the CVS Premium Packed Meal Products Based on the Kano Model (Kano 모형을 이용한 편의점 프리미엄 도시락의 서비스품질 속성 분석)

  • Baek, Seung Hee;Lee, Bumjun
    • Journal of the Korean Society of Food Culture
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    • v.34 no.3
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    • pp.308-315
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    • 2019
  • This study examined the service quality attributes of the CVS (Convenient Store) packed meal products by applying the Kano model. The main survey was conducted by a research company using an online survey in the form of a self-administered questionnaire. The 499 samples were limited to customers who had experienced CVS packed meal products within the last months. A total of 14 service quality attributes of the CVS premium packed meal products were categorized as four attractive qualities, one must-be quality, three one-dimensional qualities and six indifferent qualities. According to the Better and Worse quotient designed by Timko, 'coupon and sales promotion event', 'healthy menu configuration', and 'balanced nutrition' had higher levels of the Better coefficient. On the other hand, 'hygiene and cleanliness', 'the delicious food', and 'fresh ingredients' showed a higher Worse coefficient. The results also suggest that each service quality attribute is not a single dimension but a complex attribute. As a result, this research suggests that marketing practitioners should immediately identify consumer's changing perception of quality to attract new customers and prevent existing customers from leaving in the CVS premium packed meal products segment.

An upcycling project in textile and fashion design

  • CINI, Cigdem Asuman
    • The Research Journal of the Costume Culture
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    • v.27 no.1
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    • pp.11-19
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    • 2019
  • The mass production after the industrialisation and the fast changing fashion cycles in today's world resulted in buying clothes and home textiles more than we need and discarding them before they complete their life cycles. This causes vast amounts of textile waste that creates environmental issues. Upcycling is the creative process of transforming clothing and textile waste by reusing deadstock or used fabric to create new garments and products. It holds importance in terms of sustainability, reducing waste and environmental pollution. During the process of upcycling, certainly the creativity and innovation are the key words because to reuse a product to a better value needs a creative mind, aesthetic consciousness, innovative look and knowledge and it is quite different from a normal design procedure. There is a delicate level of aesthetics which carries the reused materials to a higher value. The handling of the materials, knowing how to manipulate the waste material, the techniques available to apply to surfaces, the concept of two and three dimension on textiles and clothing, contributions of other branches of art such as sculpture and painting all help the designer to reach a higher aesthetic value in the upcycled product in this process. In the study; it was aimed to raise awareness, to attract attention to sustainable fashion and also to contribute to sustainable development as an upcycling design project realized with students in textile and fashion design education taken as an example.

X-ray grayscale lithography for sub-micron lines with cross sectional hemisphere for Bio-MEMS application (엑스선 그레이 스케일 리소그래피를 활용한 반원형 단면의 서브 마이크로 선 패턴의 바이오멤스 플랫폼 응용)

  • Kim, Kanghyun;Kim, Jong Hyun;Nam, Hyoryung;Kim, Suhyeon;Lim, Geunbae
    • Journal of Sensor Science and Technology
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    • v.30 no.3
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    • pp.170-174
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    • 2021
  • As the rising attention to the medical and healthcare issue, Bio-MEMS (Micro electro mechanical systems) platform such as bio sensor, cell culture system, and microfluidics device has been studied extensively. Bio-MEMS platform mostly has high resolution structure made by biocompatible material such as polydimethylsiloxane (PDMS). In addition, three dimension structure has been applied to the bio-MEMS. Lithography can be used to fabricate complex structure by multiple process, however, non-rectangular cross section can be implemented by introducing optical apparatus to lithography technic. X-ray lithography can be used even for sub-micron scale. Here in, we demonstrated lines with round shape cross section using the tilted gold absorber which was deposited on the oblique structure as the X-ray mask. This structure was used as a mold for PDMS. Molded PDMS was applied to the cell culture platform. Moreover, molded PDMS was bonded to flat PDMS to utilize to the sub-micro channel. This work has potential to the large area bio-MEMS.