• 한국발생생물학회지:발생과생식
• /
• 제18권1호
• /
• pp.43-49
• /
• 2014

Three species of Nortamea concinua (NC) and Haliotis discus hannai (HDH) from Tongyeong and Sulculus diversicolor supertexta (SDS) are widely distributed on the coast of the Yellow Sea, southern sea and Jeju Island in the Korean Peninsula under the innate ecosystem. There is a need to understand the genetic traits and composition of three mollusk species in order to evaluate exactly the patent genetic effect. PCR analysis was performed on DNA samples extracted from a total of 21 individuals using seven decamer oligonucleotides primers. Seven primers were shown to generate the unique shared loci to each species and shared loci by the three species which could be clearly scored. A hierarchical clustering tree was constructed using similarity matrices to generate a dendrogram, which was facilitated by the Systat version 10. 236 specific loci, with an average of 56.3 per primer, were identified in the NC species. 142 specific loci, with an average of 44.7 per primer, were identified in the HDH species. Especially, 126 numbers of shared loci by the three species, with an average of 18 per primer, were observed among the three species. Especially, the decamer primer BION-75 generated 7 unique loci to each species, which were identifying each species, in 700 bp NC species. Interestingly, the primer BION-50detected 42 shared loci by the three species, major and/or minor fragments of sizes 100 bp and 150 bp, respectively, which were identical in all samples. As regards average bandsharing value (BS) results, individuals from HDH species (0.772) exhibited higher bandsharing values than did individuals from NC species (0.655). In this study, the dendrogram obtained by the seven decamer primers indicates three genetic clusters: cluster 1 (CONCINNA 01~CONCINNA 07), cluster 2 (HANNAI 08~HANNAI 14), cluster 3 (SUPERTEXTA 15~SUPERTEXTA 21). Comparatively, individuals of HDH species were fairly closely related to that of SDS species, as shown in the hierarchical dendrogram of genetic distances.

• 한국환경복원기술학회지
• /
• 제13권6호
• /
• pp.107-115
• /
• 2010

This study was surveyed to identify changes of flora during three years after restoration in Cheonggyecheon stream. There are four sections in Cheonggyecheon. One and two sections are upper streams and three and four sections are down streams. It was surveyed 328 species in 2006. In 2007 and 2008, 446 and 444 species were found, respectively. This result shows that Cheonggyecheon is unstable initial condition in restored stream ecosystem. Naturalized species were 58 species in 2006 and it was respectively 61 and 63 species in 2007 and 2008. Hazard species of ecosystem were three common species during survey period. In appearance of flora per section, three and four sections constituted by natural sites such as point bars, wide flood plains, riffles and ponds, marshes, etc. were surveyed more species than one and two constructed by concrete materials and narrow flow channel. Recently, as time goes by, introduced species are being increased. And succession has mainly been progressed by one year or binary herbs and perennial herbs. Compared with other restored streams, Cheonggyecheon showed more flora than Yangjaecheon and Anyangcheon. It is judged owing to length of surveyed site, various planted species and area of inhabitation space. To manage restored stream ecosystem, monitoring is essential. Further, because change of vegetation after restoration in Cheonggyecheon is very important, continuous monitoring about Flora and Naturalized species and Hazard species of ecosystem is also very important.

• Animal Systematics, Evolution and Diversity
• /
• 제25권3호
• /
• pp.295-300
• /
• 2009

We describe three new species of the Genus Kleidotoma (Figitidae: Eucoilinae) from Korea. They are K. convexitas n. sp., K. debilifovea n. sp. and K. longirudis n. sp. A key to all Korean Kleidotoma species including three new species herein are given. Descriptions and illustrations of diagnostic characters of three new species are also provided.

• 한국약용작물학회지
• /
• 제13권2호
• /
• pp.121-125
• /
• 2005

An analysis of RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) was performed with three Angelica species (A. gigas Nakai, A. sinensis (Olive.) Diels and A. acutiloba Kitag) in an effort to distinguish between members of these three species. Two arbitrary primers (OPC02, OPD11) out of80 primers tested, produced 17 species-specific fragments among the three species. Eight fragments were specific for A. sinensis, four fragments specific for A. gigas, five specific for A. acutiloba. When primers OPC02 and OPD11 were used in the polymerase chain reaction, RAPD-PCR fragments that were specific for each of the three species were generated simultaneously. Primer OPC02 produced eight species-specific fragments: four were specific for A. sinensis, one for A. gigas, and three for A. acutiloba. Primer OPD11 produced nine speciesspecific fragments: four for A. sinensis, three for A. gigas, and two for A. acutiloba. The RAPD-PCR markers that were generated with these two primers should rapidly identify members of the three Angelica species. The consistency of the identifications made with these species-specific RAPD-PCR markers was demonstrated by the observation that each respective marker was generated from three accessions of each species, all with different origins. We also performed the RAPD-PCR analysis with the dried Angelica root samples that randomly collected from marketed and from the OPC02 primer, obtained a A. gigasspecific band and the band were cloned and sequenced.

• Mycobiology
• /
• 제33권3호
• /
• pp.150-157
• /
• 2005

The fungal flora decomposing rice straw were investigated all over the soil of Sharkia Province, east of Nile Delta, Egypt, using the nylon net bag technique. Sixty-four straw-decomposing species belonging to 30 genera were isolated by the dilution plate method in ground rice straw-Czapek's agar medium at pH 6. The plates were incubated separately at $5^{\circ}C,\;25^{\circ}C\;and\;45^{\circ}C$, respectively. Twenty nine species belonging to 14 genera were isolated at $5^{\circ}C$. The most frequent genus was Penicillium (seven species), and the next frequent genera were Acremonium (three species), Fusarium (three species), Alternaria, Chaetomium, Cladosporium, Mucor, Stachybotrys (two species) and Rhizopus stolonifer. At $25^{\circ}C$, 47 species belonging to 24 genera were isolated. The most frequent genus was Aspergillus (nine species), and the next frequent genera were ranked by Penicillium (five species), Chaetomium (three species), Fusarium (three species). Each of Alternaria, Cladosporium, Mucor, Myrothecium and Trichoderma was represented by two species. At $45^{\circ}C$, 15 species belonging to seven genera were isolated. These were seven species of Aspergillus, two species of Chaetomium and two species of Emericella, while Humicola, Malbranchea, Rhizomucor and Talaromyces were represented by one species respectively. The total counts of fungi the genera, and species per gram of dry straw were significantly affected by incubation temperature and soil analysis (P < 0.05).

• 식물분류학회지
• /
• 제53권2호
• /
• pp.110-125
• /
• 2023

On the Korean Peninsula, eleven species of the genus Liparis (Orchidaceae) have been recorded, and three species are presumed to belong to the L. makinoana complex. These are L. koreana, L. japonica, and L. makinoana. Liparis japonica and L. makinoana are distributed in South Korea, but the distribution of L. koreana has not been confirmed. Liparis japonica and L. makinoana are morphologically very similar, making it difficult to classify them. Recently, L. japonica and L. makinoana in Japan were reclassified into three species. One of them is an amended species of L. makinoana, and the other two species were described as new species, specifically L. longiracemosa and L. suzumushi. It was confirmed that the three species are distributed in South Korea. Here, we provide a distribution map, photographs of the three new species, and a comparison of their morphological characteristics.

• 한국조경학회지
• /
• 제28권5호
• /
• pp.76-86
• /
• 2000

The purpose of this study is to fiure out the floral changes during three years after construction of the Youido Ecology Park. Yearly change of plant species composition, distributed at the research field, had been investigated for three years since 1996. To compare and investigate the changes of the establishment of the Ecology Park, we have analyzed the distribution characteristics of plant species, exotic naturalized species, and so on. The results obtained through this investigation are as follows; 1) Total numbers of plant species were 106, 170, 175, and 148, in the year of 1996, 1998 1999, and 2000, respectively. While the most of cultivated species before th establishment were decreased, the naturalized species such Phargmites communis Trin, and Artemisia selengensis Turcz. etc, were increased. 2) Forty three species of exotic naturalized species were investigated and were increased at the early stage of the establishment, but showed decreasing trend recently. 3) Sixty five species of introduced and planted species were investigated. Most of them were occurred at the first year after the establishment, but since 1999, there has been a significant decrease. 4) Sixty seven species among total 235 were verified as the wetland-adapted plants. The ratio to the total numbers were low, but the occupied area of a few species was expanded. Therefore, it indicates that monitoring on the physical environments of the Youido Ecology Park was urgently required.

• Mycobiology
• /
• 제34권1호
• /
• pp.34-37
• /
• 2006

Growth responses of Zea mays and Glycine max to colonization by mixture of combination of three species of arbuscular mycorrhizal (AM) fungi, two species of Glomus and a species of Scutellospora were compared. In Zea mays, plants inoculated with single species of AM fungi showed significantly higher in dry weight than non-mycorrhizal plant for all three AM fungal species. Also, growth of plants inoculated with spores of two species of AM fungi was significantly higher than nonmycorrhizal control except for plants inoculated with two Glomus species. When three species of AM fungi were inoculated, the plants showed the highest growth. In Glycine max, plants with single AM fungal species inoculation were not significantly different in plant growth from nonmycorrhizal plants. When the plants were inoculated with combination of two or more AM fungal species, their growth significantly increased compared to nonmycorrhizal plants. In both plant species, mycorrhizal root colonization by Scutellospora species was significantly lower than by Glomus species.

• 한국발생생물학회지:발생과생식
• /
• 제20권4호
• /
• pp.379-385
• /
• 2016

Seven oligonucleotides primers were shown to generate the shared loci, specific loci, unique shared loci to each species and shared loci by the three species which could be obviously scored. In the present study, 7 oligonucleotides primers produced 401 total loci in the Styela clava (SC) species, 390 in the Halocynthia roretzi (HR) and 434 in the Styela plicata (SP), respectively. Seven oligonucleotides primers generated 275 specific loci in the SC, 341 in the HR and 364 in the SP species, respectively. The oligonucleotides primer BION-23 generated 28 unique loci to each species in the SP species. Especially, the oligonucleotides primer BION-25 produced 7 unique loci to each species, which were identifying each species in the SP species. BION-17 distinguished 21 shared loci by the three ascidian species, major and/or minor fragments of sizes, which were identical in almost all of the samples. Based on the average bandsharing values of all samples, the similarity matrix ranged from 0.519 to 0.774 in the SC species, from 0.261 to 0.683 in the HR species and from 0.346 to 0.730 in the SP species. As regards average bandsharing value (BS) results, individuals from SC species ($0.661{\pm}0.081$) exhibited higher bandsharing values than did individuals from HR species ($0.555{\pm}0.074$) (P<0.05). The dendrogram obtained by the seven oligonucleotides primers indicates three genetic groups. In three ascidian species, the shortest genetic distance (0.071) exhibiting significant molecular difference was also between individual no. 20 and no. 21 within the SP species.

• Parasites, Hosts and Diseases
• /
• 제21권1호
• /
• pp.118-124
• /
• 1983

The distribution, external morphology, radula, chromosome numbers of Planorbidae snails were studied. 1. The specimens were collected at four stations in Nonsangun, Kongjugun, and Daedukgun which are located around Geum river. Three genera and three species of Planorbidae, Hippeutis cantorir Segmentina hemisphaerula and Gyraulus cenvexiusculus, were collected. H. cantori was the most abundant species among the three species. G. convexiusculus was the least abundant one. 2. Each species could be identified on the basis of its external characteristic, since the periphery of each species has a peculiar shape. H. cantori was the largest one among the three species. 3. The radula formula of each species was very similar to other species. The size of radula was proportional to the size of shell. The radula formulae of H. cantori, S. hemisphaerula, and G. convexiusculus were 29 : 1 : 29, 23 : 1 : 23, and 16 : 1 : 16 respectively. The difference of radula formula could be found in the total numbers of laternal and marginal teeth. 4. The haploid chromosome number of H. cantori was eighteen (n=18), S. hemisphaerula and G. convesiusculus were assumed to be same in their chromosome numbers (n: 18).