• The Plant Pathology Journal
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• 제40권1호
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• pp.40-47
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• 2024

Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2004년도 제22회 춘계학술대회논문집
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• pp.408-413
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• 2004

Numerical and experimental investigation were car-ried out to clarify the flow structure of underexpanded jet from a square nozzle. The square nozzle rep-resents one of the clustered combustors of a linear aerospike engine. From the numerical results, the three-dimensional shock wave of the underexpanded square jet was found to be composed of two shocks. One is the intercepting shock which corresponds to the shock observed in two-dimensional planar jet. The other is the recompression shock divided into two types. The expansion fans coming from the nozzle edges interact with each other at the comers of the nozzle exit, and overexpanded regions are generated. Therefore one of the two recompression shocks is formed at the comers of the nozzle exit behind the overexpanded regions. As the jet goes downstream, the overexpanded regions grow larger to coalesce at the symmetry planes. Then, the other type of the recompression shock is generated. The three-dimensional shock structure formed by the intercepting shock and the recompression shocks dominates the expansion of the jet boundary. The shock detection algorithm us-ing CFD results was developed to reveal the relation between the shock waves and the jet boundary, and it was found that the cross-sectional jet shape becomes cross-shape. The key features observed in the numerical investigation were verified by the experimental results. The shock structure at the diagonal plane was in good agreement with the experimental schlieren images. Moreover, the cross-sections visualized by the Mie scattering method confirmed that the cross-section of the jet becomes cross-shape.

• 한국자동차공학회논문집
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• 제6권5호
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• pp.86-96
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• 1998

A numerical study has been carried out of three-dimensional turbulent flows around a MIRA reference vehicle model both with and without wheels in computation. Two convective difference schemes with two k-$\varepsilon$ turbulence models are evaluated for the performance such as drag coefficient, velocity and pressure fields. Pressure coefficients along the surfaces of the model are compared with experimental data. The drag coefficient, the velocity and pressure fields are found to change considerably with the adopted finite difference schemes. Drag forces computed in the various regions of the model indicate that design change decisions should not rely just on the total drag and that local flow structures are important. The results also indicate that the RNG model with the QUICK scheme predicts fairly well the tendency of velocity and pressure fields and gives more reliable drag coefficient rather than the other cases.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2005년도 추계학술대회 논문집
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• pp.69-73
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• 2005

Three-dimensional density distributions of an impinging and eccentric flame have been analyzed numerically and experimentally by a combined optical system with a digital speckle tomography. The flame has been ignited by premixed butane/air from air holes and impinged vertically against a plate located at the upper side of tile burner nozzle. In order to compare with experimental data, computer synthesized phantoms of impinging and eccentric flames have been made and reconstructed by a developed three-dimensional multiplicative algebraic reconstruction technique (MART). A new scanning technique has been developed for the analysis of speckle displacements to investigate wall jet regions of the impinging flame including sharp variation of the flow direction and pressure gradient. The reconstructed temperatures have been compared with a temperature photography by an infrared camera and results of numerical analysis using a finite-element method.

• 소성∙가공
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• 제3권2호
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• pp.202-214
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• 1994

An upper bound elemental technique(UBET) is applied to predict the forging load and die-cavity filling for non-axisymmetric ring forging. In order to analyze the process easily, it is suggested that the finial product is divided into three different deformation regions. That is axisymmetric part in corner, lateral plane-strain part and shear deformation on boundaries between them. the place-strain and axisymmetric part are combinded by building block method. Also the total energy is computered through combination of three deformation part. Experiments have been carried out with pure plasticine billets at room temperature. The theoretical predictions of the forging load and the flow pattern are in good agreement with the experimental results.

• 한국전산유체공학회지
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• 제15권4호
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• pp.53-59
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• 2010

Preliminary design of a ground plate, a device installed close to the aircraft model for wind tunnel test to simulate the ground effect, was performed by a numerical simulation. A two-dimensional numerical study was performed initially to decide the optimal leading edge and flap configurations. Then, three-dimensional studies were conducted to decide the optimal flap deflection angle for pressure distribution reduction since the plate and the plate supporting system generate static pressure difference between the upper and lower flow regions. Three-dimensional simulation additionally studied the effect of the clearance between the plate and the wind tunnel side wall. For the efficiency of computation, half model was simulated and a symmetric boundary condition was applied on the center plane. Based on the preliminary design, a ground plate was designed, manufactured and tested at the Korea Aerospace Research Institute(KARI) wind tunnel. The measured pressure differences versus flap deflection angle agreed well with the predicted results.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 1998년도 춘계 학술대회논문집
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• pp.156-161
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• 1998

This describes a numerical method for predicting the incompressible unsteady laminar three-dimensional flows of fluid behaviour with free-surface. The elliptic differential equations governing the flows have been linearized by means of finite-difference approximations, and the resulting equations have been solved via a fully-implicit iterative method. The free-surface is defined by the motion of a set of marker particles and interface behaviour was investigated by way of a 'Lagrangian' technique. Using the GALA concept of Spalding, the conventional mass continuity equation is modified to form a volumetric or bulk-continuity equation. The use of this bulk-continuity relation allows the hydrodynamic variables to be computed over the entire flow domain including both liquid and gas regions. Thus, the free-surface boundary conditions are imposed implicitly and the problem formulation is greatly simplified. The numerical procedure is validated by comparing the predicted results of a periodic standing waves problems with analytic solutions or experimental results from the literature. The results show that this numerical method produces accurate and physically realistic predictions of three-dimensional free-surface flows.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 1994년도 춘계학술대회 논문집
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• pp.63-70
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• 1994

An upper bound elemental technique(UBET) is applied to predict forging load and die-cavity filling for non-axisymmetric ring forging. The finial product is divided into three different deformation regions. That is axisymmetric part in corner, lateral plane-strain part and shear deformation on boundaries between them. The plane-strain and axisymmetric part are combinded by building block method. Also the total energy is computered through combination of three deformation part. Experiments have been carried out with pure plasticine billets at room temperature. The theoretical predictions of the forging load and the flow pattern are in good agreement with the experimental results.

• 한국전산유체공학회지
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• 제20권2호
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• pp.1-6
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• 2015

In this study, a numerical prediction on propulsive performance of a ducted propeller in open water condition was carried out by solving Reynolds averaged Navier-Stokes(RANS) equation using computational fluid dynamics(CFD). A configuration of propeller Ka-470 inside duct 19A was considered. Hexahedral grid system was generated by dividing whole computational domain into three separate regions; propeller, duct and outer flow region. A commercial CFD software, ANSYS-CFX was used for numerical simulations. Results were compared with experimental data and showed considerable improvement in accuracy, in comparison to those from surface panel method which is based on potential flow assumption. The results also exhibited the importance of grid system within the gap between the inner surface of duct and blade tip for accurate prediction of propulsive performance of ducted propeller.

• 대한기계학회논문집B
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• 제28권9호
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• pp.1015-1021
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• 2004

Micro-resolution Particle Image Velocimetry(Micro-PIV) was used to measure the flow in a micro-branch(Micro-Bypass). In this paper, effects of particle lump at the tip of a Micro-branch and difficulties of Micro-PIV measurements for microfluidics with branch passage were described. Micro-bypass was composed of a straight channel(200(100)${\mu}$m width ${\times}$ 80${\mu}$m height) and two branches which has 100(50)${\mu}$m width ${\times}$ 80${\mu}$m height. One of branches was straight and the other was curved. Experiments were performed at three regions along streamwise direction(entrance, middle and exit of branch) and five planes along vertical direction (0, ${\pm}$10, ${\pm}$20 ${\mu}$m) for the range of Re=0.24, 1.2, 2.4. Numerical simulation was done to compare with the measurements and understand the effects of particle lump at the tip of branch. And another fluid(3% poly vinyl Alcohol aqueous solution) were adapted for this study, so there were no particle sticking. In this case, we could get velocity difference between straight and curved branches.