• 현장농수산연구지
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• 제18권1호
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• pp.25-28
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• 2016

말의 복지는 생리적 및 심리적 필요의 만족에 좌우된다. 본 연구의 목적은 말목장에서 펜스의 안전을 평가하기 위함이다. 행동장애(Cribbing)를 가진 Thoroughbred 수망아지 한 마리가 패독에서 폐사한 채 발견되었다. 패독의 펜스는 4개의 파이프로 만들어졌고, 망아지는 위에서 2번째 파이프를 무는 행동장애를 하였다. 그러나 그 망아지는 얼굴을 빼지 못하였는데 그 이유는 아래턱이 1번째 파이프에 걸렸고 앞니가 2번째 파이프에 끼었기 때문이다. 그래서 망아지는 얼굴을 빼기 위해서 사투하는 동안에 당황하였고, 쓰러졌으며 결국 교사하였다. 안전펜스는 말목장에 필수적이고 그 기능은 말을 보호하는 것이지만 위해를 가하였다. 말의 안전한 관리를 위하여 말 얼굴길이와 파이프의 간격을 고려하여야한다. 말이 패독에 있을 때 입마개를 씌울 필요가 있는지 더 연구가 필요하다. 본 연구는 말산업에서 펜스타입과 안전성평가에 중요한 벤치마크를 제공하였다.

• Molecules and Cells
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• 제40권10호
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• pp.796-804
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• 2017

Endogenous retroviruses (ERVs) have been integrated into vertebrate genomes and have momentously affected host organisms. Horses (Equus caballus) have been domesticated and selected for elite racing ability over centuries. ERVs played an important role in the evolutionary diversification of the horse genome. In the present study, we identified six equine ERV families (EqERVs-E1, I1, M2, P1, S1, and Y4), their full-length viral open reading frames (ORFs), and elucidated their phylogenetic relationships. The divergence time of EqERV families assuming an evolutionary rate of 0.2%/Myr indicated that EqERV-S3 (75.4 million years ago; mya) on chromosome 10 is an old EqERV family and EqERV-P5 (1.2 Mya) on chromosome 12 is a young member. During the evolutionary diversification of horses, the EqERV-I family diverged 1.7 Mya to 38.7 Mya. Reverse transcription quantitative real-time PCR (RT-qPCR) amplification of EqERV pol genes showed greater expression in the cerebellum of the Jeju horse than the Thoroughbred horse. These results could contribute further dynamic studies for horse genome in relation to EqERV gene function.

• 생명과학회지
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• 제18권3호
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• pp.369-373
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• 2008

Staphylococcus intermedius (S. intermedius) has been rarely isolated from horse. In this study, we investigated the antimicrobial susceptibility patterns of S. intermedius isolated from skin lesions of Thoroughbred horse in Daegu Equestrian Association. The skin lesions were showed with dehairing and slight purulent inflammation. Bacteria were isolated from skin lesions and identified as S. intermedius by biochemical tests and MicroLog (BIOLOG, California, USA). Antimicrobial susceptibility test of S. intemedius isolates was performed with 33 antimicrobial agents (BBL, Maryland, USA) by using the agar disk diffusion method. It showed a high sensibility in the amikacin, amoxycillin/clavuramic acid, ampicillin, cefoxitin, cephalothin, chloramphenicol, ciprofloxacin, enrofloxacin, florfenicol, kanamycin, neomycin, nitrofurantoin, norfloxacin, ofloxacin, oxacillin, penicillin G, tetracycline and vancomycin. The horse was treated with penicillin, and cured completely after two weeks. The present results showed valuable information for treatment and prevention of skin disease in horse.

• 한국임상수의학회지
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• 제26권1호
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• pp.69-71
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• 2009

A 2-year-old, female Thoroughbred racehorse was referred to the Equine Hospital of Korea Racing Authority. The horse was presented with fluctuant swelling at the base of the left temporal ear tooth. We performed physical examination, radiography and ultrasonography, and diagnosed as dentigerous cyst on the left temporal ear tooth-(dentigerous cyst). The horse was treated with removal of a dentigerous cyst(aural fistula). After surgical procedure for 12 days, the patient was recovered completely.

• 한국임상수의학회지
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• 제26권3호
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• pp.286-289
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• 2009

Perineal laceration commonly occurs due to dilation of the birth canal at the time of foaling in primiparous mares. A 7-year-old Thoroughbred mare was presented with faecal contamination of the external genitalia. She was having history of constant straining three days after the mare's first foaling. Physical examination revealed the tearing extended from the deep part of vagina through muscles of the perineal body involved rectal floor and anal sphincter. Tentative diagnosed was third-degree perineal laceration therefore one-stage surgical procedure and a modified surgical procedure at three-week intervals was performed. During postoperative care for 3 weeks, soft faces was a key factor in success. After 3 weeks, the patient was recovered to normal condition completely.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.350-356
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• 2019

Objective: To examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide. Methods: The mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skeletal muscle tissue was confirmed using quantitative-reverse transcriptase polymerase chain reactions (qRT-PCR). We then extracted horse muscle cells from the skeletal muscle tissue of a neonatal Thoroughbred. Myokine expression after hydrogen peroxide treatments was confirmed using qRT-PCR in horse skeletal muscle cells. Results: IL-6, IL-8, CXCL2, and CCL4 expression in Thoroughbred and Jeju horse skeletal muscles significantly increased after exercise. We stably maintained horse skeletal muscle cells in culture and confirmed the expression of the myogenic marker, myoblast determination protein (MyoD). Moreover, myokine expression was validated using hydrogen peroxide ($H_2O_2$)-treated horse skeletal muscle cells. The patterns of myokine expression in muscle cells were found to be similar to those observed in skeletal muscle tissue. Conclusion: We confirmed that several myokines involved in inflammation were induced by exercise in horse skeletal muscle tissue. In addition, we successfully cultured horse skeletal muscle cells and established an in vitro system to validate associated gene expression and function. This study will provide a valuable system for studying the function of exercise-related genes in the future.

• 한국임상수의학회지
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• 제35권3호
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• pp.88-92
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• 2018

Osteochondroma (OC) is a cartilage-capped exostosis. In horses, OC commonly develops on the caudal distal metaphysis of the radius (CDMR). The purpose of study was to describe the outcomes of arthroscopy for the treatment of OC on CDMR. Diagnosis was based on clinical signs (lameness and distention of carpal sheath), radiography (location and size of OC), and ultrasonography (location of OC, torn deep digital flexor tendon, fibrin, and effusion of carpal sheath). Arthroscopy was performed on 68 Thoroughbred horses with OC on CDMR. Sixty of the 68 cases showed deep digital flexor tendinitis as a result of sharp protuberances of the OC. All horses survived, and 62 of the 68 cases returned to athletic function (racing) after arthroscopy. The present study demonstrated that arthroscopy is useful for treating OC of CDMR in horses.

• 한국동물위생학회지
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• 제27권2호
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• pp.153-157
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• 2004

Bacteria isolated from nasal cavity of 50 Thoroughbred horses with respiratory tract infection were examined. There were isolated Pseudomonas aeruginosa(33.5%), Escherichia coli(10.2%), Pseudomonas spp(7.6%), Klebsiella oxytoca(5.9%), Streptococcus equi subsp zooepidemious(6.2%), Klebsiella pneumoniae(3.4%), Acinetobacter spp(5.5%) and coagulase negative staphylococcus(2.1%). The majority of isolates were highly susceptible to amikacin, amoxicillin, aztreonam, cefotaxime, cefepime, cefotetan, ceftazidime, cefuroxime, chloramphenicol, ciprofloxacin, clindamycin, erythromycin, gentamicin, imipenem, tetracyclin and vancomycin. These results can provide basic information for the treatments of respiratory tract infections in Thoroughbred horses.

• Animal Bioscience
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• 제35권4호
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• pp.527-532
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• 2022

Objective: In this study, we aimed to investigate the recent changes such as allele frequencies and total probability of exclusion (PE) in Thoroughbred horses in Korea using short tandem repeat (STR) parentage panels between 2006 and 2016. Methods: The genotype was provided for 5,988 horse samples with 15 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3 and VHL20). Results: In our study, the observed number of alleles per locus ranged from 3 (HMS1) to 9 (ASB17) in 2006 and 4 (HMS1) to 9 (ASB2) in 2016, with a mean value of 6.28 and 6.40, respectively. Of the 15 markers, HMS2, HTG4, and CA425 loci had relatively low polymorphism information content (<0.5000) in the Thoroughbred population. Mean levels of genetic variation in 2006 and 2016 were observed heterozygosity (H_O) = 0.708, and expected heterozygosity (H_E) = 0.685, as well as and H_O = 0.699 and H_E = 0.682, respectively. The PE was calculated for each group based on the allele frequencies of 14 or 15 STRs. The 2006 survey analyzed that PE was 0.9998, but it increased to 0.9999 in 2016 after the HMS2 marker was added in 2011. The current STR panel is still a powerful tool for parentage verification that contributes to the maintenance of integrity in the Thoroughbred population. Conclusion: The current STR panel is still a powerful tool for parentage verification that contributes to the maintenance of integrity in the Thoroughbred horses. However, continuous monitoring genetic variability is necessary.

• 한국임상수의학회지
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• 제38권5호
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• pp.244-248
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• 2021

A 10-year-old Thoroughbred mare was referred to the Jeju National University Equine Hospital with roaring, dyspnea, and weight loss. On endoscopic examination, the horse was diagnosed with right arytenoid chondritis. Surgical treatment was selected due to the failure of a previous medical treatment. Permanent tracheostomy was performed in a standing position. The horse was restrained and tied in a proper position in the stock. The cranial parts of the 2nd to 5th tracheal cartilages were resected, as were the associated skin, mucosa, muscle, and cartilages. After the stoma was formed, external mucosa and skin were sutured using a simple interrupted method. The horse was hospitalized for 22 days receiving postoperative care including antibiotics, non-steroidal anti-inflammatory drugs, dressing as required, and was pregnant six months after the surgery. A permanent tracheostomy is thought to be effective in horses with diseases causing upper respiratory tract obstruction.