• Korean Journal of Microbiology
• /
• v.10 no.4
• /
• pp.167-174
• /
• 1972

Effects of 13 organic compounds including glucose, fructose, xylose, glutamate, succinate, malate, glycine, lactate, acetate, pyruvate, citrate, formate and cis-aconitate on the oxidation of thiosulfate and the availability of these compounds as the substrate for the respiration by Thiobacillus ocncretivorus, which is known to be an obligated autotroph, were studied. Malate nad glycine at 0.5 per cent concentration nearly doubled the thiosulfate oxidation compared to the control. No other organic substances enhanced the thiosulfate oxidation compared to the control. No other organic substances enhanced the thiosulfate oxidation. Moreover, some 30 to 40 per cent decrease was recorded by fructose, sulfate-salts medium, some 30 to 40 per cent decrease was recorded by fructose, citrate, xylose, malate, flucose, glutamate and succinate. No respiration could occur when formate and pyruvate were supplied as the substrate for respiration. But it was obvious that flucose, fructose, xylose, glutamate, malate, citrate and succinate could be used as the substrate for respiration to some extent, regarding the fact that some increase in respiration rates could be recorded compared to the result from the salts medium, where neither thiosulfate nor orgnic compounds were added. Thus, it was postulated that this organism could possibly be converted into mixotroph or hetrotroph if appropriate conditions could be prepared.

• Resources Recycling
• /
• v.26 no.2
• /
• pp.39-45
• /
• 2017

In order to exclude the effect of uneven distribution of gold in anode slime, the dissolution of gold and silver from the metal mixture was investigated in different systems, such as the mixture of hydrochloric acid and oxidizing reagents ($H_2O_2$, NaClO and $HNO_3$), thiosulfate and thiourea. In the mixture of HCl and either $HNO_3$ or $H_2O_2$, Au was completely dissolved but the leaching percentage of Ag was around 1%. In both thiosulfate and thiourea solution, gold was not dissolved at all. The presence of ferric ion in acidic thiourea solution showed a favorable effect on the leaching of silver but further study is necessary to elucidate the combined effect of ferric ion and sulfuric acid.

• Proceedings of KOSOMES biannual meeting
• /
• 2017.11a
• /
• pp.278-278
• /
• 2017

• Plant Resources
• /
• v.3 no.1
• /
• pp.59-65
• /
• 2000

The opening process of cut calla flower was faster at 30 t than at lower temperatures as it could be expected from its tropical origin. Gibberellin enhanced the flower opening, however, it also speeded up senescent. Silver thiosulfate was effective in prolonging the vase life of the cut calla flower. Silver thiosulfate reduced ethylene generation by the flower and inhibited microbial growth in the flower stalk. Reduction in ethylene generation and inhibition of microbial growth is thought to be responsible for the extension of the vase life of cut calla flowers by silver thiosulfate.

• 한국전기화학회:학술대회논문집
• /
• 1999.10a
• /
• pp.38-38
• /
• 1999

• 한국전기화학회:학술대회논문집
• /
• 1999.04a
• /
• pp.44-44
• /
• 1999

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2000.10a
• /
• pp.128-135
• /
• 2000

The thiosulfate reductase gene (PhsABC) from Salmonella typhimurium was expressed in Escherichia coli in order to produce sulfide from inorganic thiosulfate and precipitate metals as metal sulfide complexes. A 5.1-kb DNA fragment containing the native phsABC and a 3.7-kb DNA fragment, excluding putative promoter and regulatory regions were inserted into expression vectors pTrc99A and pJB866, respectively. Upon expression of phsABC, E. coli DH5$\alpha$ harboring the phsABC constructs showed higher thiosulfate reductase activity and produced significantly more sulfide than the control strain (E. coli DH5$\alpha$) under both aerobic and anaerobic conditions. Among the four constructs, E. coli DH5$\alpha$ harboring pSB74 produced the highest level of thiosulfate reductase and removed most of heavy metals from solution under anaerobic conditions. In a mixture of 100 $\mu$M each of cadmium, lead, and zinc, the strain could remove $99\%$ of the total metals from solution within 10 hours. Cadmium was removed first, lead second, and zinc last. In contrast, a negative control did not produce any measurable sulfide and removed very little metals from solution. These results have important implications for removal of metals from wastewater contaminated with several metals.

• Resources Recycling
• /
• v.26 no.1
• /
• pp.30-36
• /
• 2017

Leaching experiments of anode slime were performed with several inorganic acids (HCl, $HNO_3$ and $H_2SO_4$) together with thiourea and thiosulfate solution to recover gold and silver. Gold was not dissolved at all into these inorganic acids in the absence of any oxidizing agents. At the same concentration of inorganic acid, the leaching of percentage of Ag was the highest in the sulfuric acid solution. The leaching percentage of silver increased with the increase of HCl concentration owing to the formation of $AgCl_2{^-}$. Copper, nickel and zinc except tin was almost dissolved in these inorganic acids but no tin was dissolved in nitric acid solution. Most of Au and Ag were dissolved into the mixture of sulfuric acid and thiourea solution. Thiosulfate could dissolve some silver from the anode slime but no gold was dissolved by this agent.

• Journal of Yeungnam Medical Science
• /
• v.13 no.2
• /
• pp.243-250
• /
• 1996

Extravasation of toxic chemotherapeutic agents cause severe skin ulceration and necrosis which often need secondary surgical intervention. Still, there were not established antidote agent in case of extravasation with mitomycin-c. Dimethyl sulfoxide is known as an effective chemical scavenger of toxic hydroxyl free radical and sodium thiosulfate also was demonstrated significant protector from mitomycin-c induced ulceration by a few experimental studies. Author investigated necrotic area of mitomycin-c injected site and compare to the effectiveness of topical treatment with dimethyl sulfoxide and intradermal injection of sodium thiosulfate according to starting times, forty five mice were divided into 3 groups. Control group(n=5) had no treatment after subcutaneous injection of mitomycin-c. Experimental group I and II were 20 mice treated dimethyl sulfoxide and sodium thiosulfate, respectively. Depending on the starting time of treatment, group I and II were subdivided into 1, 2, 3 and 4 as immediate, 6 hours, 12 hours and 24 hours after mitomycin-c injection. Histologic studies of the necrotic area and survival area after treatment were performed using hematoxylin-eosin staining. The mean necrotic area of group I was significantly decreased depending on the starting time of treatment compared with control group(p<0.01). The results means there was no necrosis area which was treated with topical sodium thiosulfate within 6 hours, and it showed also significant decrease of necrosis area within 24 hours. There was also no necrosis area in group II-1 and significant decrease of necrosis area II-2 and III-3. But, effctiveness of intradermal injection of sodium thiosulfate was not found in group II-4 which was started after 24 hours. Hisotolgic findings showed a bland coagulative necrosis without inflammatory changes and no granulation tissue. The significant difference that cytoplasmic loss of subcutaneous fat and decrease number of hair follicles between two groups resulted from the methods of treatment by topical application and intradermal injection. In conclusion, immediate treatments with topical dimethyl sulfoxide or intradermal injection of sodium thiosulfate signifcantly prevents necrosis by extravasation of mitomycin-c.

• Journal of Environmental Health Sciences
• /
• v.21 no.3
• /
• pp.96-101
• /
• 1995

A selective and highly sensitive spectrophotometric measurements have been developed for the determination of trace mercury(II) with thiosulfate and PAR. Hg(II)-thiosulfate complex was extracted into high molecular alkylamine such as quaternary ammonium salt and back extracted into 1 M-HCl solution. The Hg(II)-PAR complex has maximum absorbance at 499 nm and obeys Beer's law in the range of 0.04~1.0 $\mu g/mL$ of mercury(II). The molar absorptivity and Sandell's sensitivity are $6.27\times 10^4 L.mole^{-1} cm^{-1}$ and $3.2\times 10^{-3}g/cm^2$ respectively.