• 한국응용과학기술학회지
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• 제15권3호
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• pp.27-31
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• 1998

Antioxodative substances in Mulberry leaves were examined. Antioxidative substances in Mulberry leaves were extracted by 80% methanol agueous solution. Antioxidative activity of extract was determined by examining hydrogen donating ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the inhibitory effect on the formation of the peroxide from Linoleic acid in the test tube at $50^{\circ}C$. Antioxidative substance were, then, separated and indentified by thin layer chromatography(TLC), UV-Vis spectrum and High performance liquid chromatography(HPLC) methods. Hydrogen donating ability on DPPH and antioxidative ability on linoleic acid of the extracted antioxidative substance were higher than those of 100ppm butylated hydroxy toluene(BHT). The extracted antioxidative substances were separated by TLC using ethylacetate : chloroform : formic acid : water(8 : 1 : 1 : 1 v/v) as a solvent, and a spot at Rf=0.35 was detected. The spot was scraped from the plate, and extrated by methanol. The extract was analyzed by UV-Vis spetra and HPLC, and chlorogenic acid was identified as a antioxidative substance.

• 약학회지
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• 제38권1호
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• pp.12-19
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• 1994

Capsaicin(8-methyl-N-vanillyl-6-nonenamide) is the principal pungent component of Capsicum fruits. This work is directed to the capsaicin-hydrolyzing enzyme playing a key role in the rate limiting and critical step of capsaicin metabolism. In order to get precise information on the enzyme's subcellular location, rat liver homogenate was divided into six subcellular fractions by differential centrifugation technique: crude nuclear pellet, PNS(post nuclear supernatant) fraction, lysosomal pellet, cytosol, Tris wash fraction, micrisomes. Capsaicin-hydrolysing enzyme activity was analysed by high performance liquid chromatography(HPLC). This enzyme was found at the highest specific activity in the microsomal fraction and co-distributed with marker enzymes of the endoplasmic reticulum, NADPH-cytochrome c reductase and nucleoside diphosphatase. This is compatible with the result of ninhydrin color reaction of vanillylamine, primary metabolite of capsaicin hydrolysis, on thin layer chromatography(TLC). This enzyme is most active at pH $8.0{\sim}9.0$. Definite subcellular location of this enzyme will make it easy to proceed with further study.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.169-172
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• 2000

Studies on extraction of red pigment was performed to provide the basic information for the utilization of red pigment as s new source of natural food colorant. A bacterium isolated from marine resources were carried out the test for excretion of red pigment. One strain of a marine bacterium, KSR-97 showed a high production of red pigment on the medium of colloidal chitin, peptone-yeast extract with minerals. In physicochemical and sensory properties in aqueous solution of red pigment was investigated at various condition of pH, temperature, concentration of ethanol and stability of storage. Potent antioxidative of red pigment was separated by thin layer chromatograpy, silica gel chromatography and reverse high performance liquid chromatography using ODS hypersil column.

• 한국산림과학회지
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• 제15권1호
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• pp.39-41
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• 1972

일반적(一般的)으로 식별(識別)이 어려운 주목속(朱木屬) 5수종(樹種)의 심재추출물(心材抽出物)을 박층(薄層) chromatography로 전개후 디아조계 염료로 발색시켜 육안(肉眼), 형광(螢光) 및 자외선(紫外線)조사로 관찰한바 주목속(朱木屬)의 심재추출물의 박층(薄層)크로마토그람이 수종간에 현저한 차이가 있어 주목속(朱木屬)의 분류를 간단한 화학적방법(化學的方法)으로 가능케 하였다. 그 박층(薄層)크로마토그람의 색깔에 의한 분류기초는 다음과 같다.

• BMB Reports
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• 제28권4호
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• pp.323-330
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• 1995

Recent studies have shown that dopamine applied to cultured swimming motor neurons of Polyorchis penicillatus produces an inhibitory action by opening potassium channels through $D_2$-like receptors. In this study, it was demonstrated that dopamine found in the hydromedusa was not from exogenous sources and the content of dopamine depended on the $Ca^{2+}$ content of the dissecting media. In addition, a combination of thin layer chromatography and high performance liquid chromatography showed the presence of DOPA and DO PAC-like compounds in the jellyfish. The glyoxylic acid method for catecholamines suggested that a population of small cells, neither swimming motor neurons nor B-like neurons, had dopaminergic systems. From all these results, it is suggested here that DA synthesized from DOPA in some cells is released. being dependent on calcium concentrations, into a synaptic cleft and degraded into DOPAC after acting as an inhibitory transmitter to swimming motor neurons.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.175-175
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• 1994

녹용의 유효성분을 분리하기 위하여 지질성분을 분석하고 그 약효를 검색하였다. Folch-Suzuki 분배법, Sephadex G-50, DEAE-Sephadex A-25 Column Chromatography, High Performance Thin Layer Chromatography, HPLC로 정제한후 Mass Spectrometer, NMR, FT-IR등을 이용하여 구조를 분석하였다. 이 물질은 분자량이 1065인 polyhydroxyunsaturated lipid임을 확인하였다. Strepthzotocin으로 당뇨병을 유발시킨 쥐에 이 물질을 투여한 후 혈액과 조직에서 생화학적 변화를 조사하였다. 정상군에 비해 투여군에서 혈당이 감소하였으나 혈액에서 insulin의 양은 증가하지 않았다. 당뇨병 쥐의 적혈구가 정상에 비해 용혈이 되지 않으나 투여군의 적혈구는 정상과 비숫한 용혈현상을 보여 주었다. 대뇌조직에서 gangliosides를 분석한 결과 당뇨병에 의해 GM1이 증가하는 양상을 보여 주었으나 투여군의 경우 대뇌 gangliosides 분포는 정상과 같았다. 대뇌 lipid bound sialic acid를 정량한 결과 당뇨병에 의해 그 양이 감소되었으나 녹용을 투여하였을 때는 정상에 비해 그 양이 더 증가하였다. Strepthzotocin으로 유발시킨 당뇨병 쥐에 녹용의 지질성분을 투여하였을 때 치료효과가 있는 것으로 추정된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제16권2호
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• pp.57-59
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• 2008

While searching for pancreatic lipase inhibitors, the active compound was found in a methanol extract of Apis mellifera venome. The active compound was isolated by Diaion HP-20 column chromatography, thin layer chromatography and HPLC. The active compound is stable to the extreme pH and heat. There is no loss of activity both in acidic and alkaline solution in the pH range of 2 to 11 by heating for 15 minutes at $90^{\circ}C$. The rf value of the compound was 0.51 at TLC with butanol : methanol: water (4:1:2) solvent system. The molecular weight of the compound was determined to be 293 by EI-MS.

• 한국미생물·생명공학회지
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• 제27권3호
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• pp.179-183
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• 1999

The volatile components (essential oil) showing antimicrobial activity were extracted from the fruit peel of Zanthoxylum piperitum DC by distillation and separated by thin layer chromatography (TLC). The crude volatile components exhibited antimicrobial activity only at very high concentration. The active fraction obtained by TLC inhibited noticeably the growth of bacteria. The minimum inhibitory concentration (MIC) of the fraction were 150ppm, 300ppm, and 300ppm against Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis, respectively. The components in the active fraction were identified by gas chromatography/mass spectrometry to be geranlyl acetate (60.23%), citronellal(36.01%), citronellol(3.77%), geraniol(0.46%), and cumin ldehyde(0.43%).

• 대한약리학회지
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• 제8권2호
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• pp.49-54
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• 1972

The component and pharmacologic action on Dianthus sinensis L. were investigated as follows: 1) The components of Dianthus sinensis L. were suggested 8 kind of components by means of thin layer chromatography and paper chromatography. The one of them were suggested as a steroidal saponin by means of IR-spectrum and Liebermann-Burchard, Salkowiski, $SbCl_3$ reaction. 2) Sodium level in serum were reduced by administration of the sample to the rabbits, while in urine were elevated. 3) Blood sugar level of normal rabbits administered with sample, no significant variation were detected. 4) When the hyperglycemic rabbits treated with alloxan were administered sample, blood-sugar level were significantly decreased than control group.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.919-927
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• 2007

Antagonistic fluorescent pseudomonads isolated from rhizospheric soil of rice were characterized by 16S rRNA amplicon and fatty acid methyl ester (FAME) analyses. Antagonistic isolates were grown in the fermentation media, and production of antibiotics was confirmed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Production of fungal cell-wall-degrading enzymes such as protease, cellulase, pectinase, and chitinase was determined. Dendrogram based on the major and differentiating fatty acids resulted into 5 clusters, viz., cluster I (P. pseudoalcaligenes group), cluster II (P. plecoglossicida group), cluster III (P. fluorescens group), cluster IV (P. aeruginosa group), and cluster V (P. putida group). Characteristic presence of high relative proportions of cyclopropane (17:0 CYCLO w7c) was observed in antagonistic bacteria. Data revealed biodiversity among antagonistic fluorescent pseudomonads associated with the rice rhizosphere. Results presented in this study will help to identify the antagonistic isolates and to determine their mechanisms that mediate antagonism against fungal pathogens of rice.