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Studies on Lipids in Fresh-Water Fishes 1. Distribution of Lipid Components in Various Tissues of Crucian Carp, Carassius carassius (담수어의 지질에 관한 연구 1. 붕어(Carassius carassius)의 부위별 지질성분의 분포)

  • CHOI Jin-Ho;RO Jae-Il;PYEUN Jae-Hyeong;CHOI Kang-Ju
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.4
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    • pp.333-343
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    • 1984
  • This study was designed to elucidate the lipid and its fatty acid composition in various tissues of fresh water fishes. The free and bound lipids in meat, skin and viscera of crucian carp (Carassius carassius) were extracted with ethyl ether and the mixed solvent of chloroform-methanol-water (10/9/1, v/v). The free and bound lipids were fractionated into neutral lipid, glycolipid and phospholipid by a silicic acid column chromatography using chloroform, acetone and methanol, respectively, and quantitatively analyzed by thin layer chromatography (TLC) and TLC scanner. The fatty acid compositions of polar ana nonpolar lipids in meat, and these of neutral lipid in various tissues were analyzed by gas liquid chromatography(GLC). The free lipid content in meat, skin and viscera was $6.22\%,\;9.95\%\;and\;9.76\%$, whereas the bound lipid content in those tissues was $10.01\%,\;3.56\%\;and\;7.36\%$, respectively. The neutral lipid contents in free lipid were ranged from $71.7\%$ to $89.4\%$, and $3{\sim}9$ times higher than those in bound lipid, while the phospholipid contents in bound lipid were ranged from $42.3\%$ to $63.2\%$, and $5{\sim}10$ times higher than those in free lipid. The neutral lipid was mainly consisted of triglyceride ($81.91{\sim}88.34\%$) in free lipid, and esterified sterol & hydrocarbon ($41.00{\sim}59.43\%$) in bound lipid. The phospholipid was mainly consisted of phosphatidyl ethanolamine($54.56{\sim}66.79\%$) and phosphatidyl choline ($21.88{\sim}34.28\%$) in free lipid, and phosphatidyl choline ($50.49{\sim}70.57\%$) and phosphatidyl ethanolamine ($15.74{\sim}24.92\%$) in bound lipid. The major fatty acids of polar lipid in free and bound lipids were $C_{16:0}\;(17.53\%,\;19.29\%)$, $C_{18:1}\;(24.57\%,\;16.08\%)$, $C_{18:2}\;(8.39\%,\;4.03\%)$, $C_{22:5}\;(1.68\%,\;8.08\%)$, and $C_{22:6}\;(6.22\%,\;13.60\%)$ and these of neutral lipid in free and bound lipids were $C_{16:0}\;(17.67\%,\;24.15\%)$, $C_{16:1}\;(12.81\%,\;5.52\%)$, $C_{18:1}\;(24.13\%,\;13.02\%)$, $C_{18:2}\;(15.47\%,\;8.68\%)$, $C_{22:5}\;(0.88\%,\;4.14\%)$ and $C_{22:6}\;(1.17\%,\;5.04\%)$, respectively. The unsaturations (TUFA/TSFA) of polar lipid in free and bound lipids were 2.02 and 2.74, and $1.5{\sim}2.0$ times higher than 1.51 and 1.23 of nonpolar lipid. In both polar and nonpolar lipids, w3 highly unsaturated fatty acid (w3HUFA) content of bound lipid was $2{\sim}5$ times higher than that of free lipid. The polyenoic acid contents such as $C_{20:5},\;C_{22:5}\;and\;C_{22:6}$ in bound lipid were $2{\sim}5$ times higher than these in free lipid. Consequently, there were significant difference between the lipid and its fatty acid composition in free and bound lipids and/or in various tissues.

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Actual Clothing Style of Middle school girls According to Self-perception of Their Body Size (여중생의 신체 인식에 따른 착의 의복형태에 관한 연구)

  • Park Woo-Mi;Wee Eun-Hah
    • Journal of Korean Home Economics Education Association
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    • v.18 no.1 s.39
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    • pp.113-125
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    • 2006
  • The purpose of this study was to examine the effects of perception of body size on actual clothing style in middle school girls. To achieve this, this study analyzed self-perception of body size, aspired clothing style, and actual clothing style. By classifying groups into thin and thick, the difference in clothing style according to groups was also analyzed. Subjects of this study were middle school girls in Gwangju and 219 responses were collected from real body measurement and questionnaires. WIN SPSS+10.0 package was used to obtain results. Results are as follows: 1. For the self-perception of body size, middle school girls perceived much thicker in the lower body than the upper body. And they were unsatisfactory about the lower body which was perceived thick. 2. In the correlation of actual and aspired clothing style of middle school girls, they wore a aspired clothing style actually. Their actual or aspired upper clothing style was to cover the skin, exposing or covering a body silhouette. Their lower clothing style was a slacks type that covers a body silhouette and the skin. 3. In selecting actual clothing styles, middle school girls were somewhat affected by self-perceived body size. But they did not prefer the clothing styles perceived thick in girth or big in width in exposing the skin or a body silhouette. On the other hand, as it was perceived long, wearing was increased. frequently wore the upper and lower clothing styles of exposing the skin or a body silhouette than the thick group. But both groups wore the clothing styles of covering the skin or a body silhouette regardless of their thinness and thickness. And when wearing the clothing style of exposing neck and shoulder, middle school girls considered actual body size more than self-perceived size. On the other hand, when wearing the clothing style of exposing arms and silhouette by fitted legs, they considered self-perceived body size more.

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Studies on the Development of Food Resources from Waste Seeds -II. Chemical Composition of Apple Seed- (폐기종실(廢棄種實)의 식량자원화(食糧資源化)에 관(關)하여 -제(第) 2 보(報) : 사과씨의 화학적(化學的) 조성(組成)-)

  • Yoon, Hyung-Sik;Choi, Cheong;Oh, Man-Jin
    • Korean Journal of Food Science and Technology
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    • v.15 no.2
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    • pp.128-132
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    • 1983
  • The apple seed contained 25.96% of crude fat and 37.62% of crude protein. The lipid fractions obtained by cilicic column chromatography were mainly composed of about 93.52% neutral lipid, whereas compound lipid was only 6.48% level. Among the neutral lipid separated by thin layer chromatography, triglyceride was 92.17%, sterol ester, sterol, diglyceride and free fatty acid were 3.53, 2.25, 1.44 and 0.56, respectively. The predominent fatty acids of total and neutral lipids were linoleic acid (59.79-69.37%) and oleic acid (20.04-29.82%), but those of glycolipid and phojspholipid were linoleic acid (29.20-36.04%). The major fatty acids of triglyceride separated from neutral lipid were oleic acid (44.31%), linoleic acid (36.66%) and palmitic acid (12.48%). The salt soluble protein of apple seed was highly dispersible in 0.02M sodium phosphate buffer containing about 1.0M $MgSO_4$, and the extractability of seed protein was 37%, Glutamic acid was the major amino acid in salt soluble protein, followed by arginine and aspartic acid. The eletrophoretic analysis showed three bands in apple seed protein, and the collection rate of the main protein fraction purified by Sephadex G-100 and G-200 was 76.6%. Glutamic acid, aspartic acid and arginine were the major amino acids of the main apple seed protein. The molecular weight for the main protein of the apple seed was estimated to be 45,000.

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Seismic wave propagation through surface basalts - implications for coal seismic surveys (지표 현무암을 통해 전파하는 탄성파의 거동 - 석탄 탄성파탐사에 적용)

  • Sun, Weijia;Zhou, Binzhong;Hatherly, Peter;Fu, Li-Yun
    • Geophysics and Geophysical Exploration
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    • v.13 no.1
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    • pp.1-8
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    • 2010
  • Seismic reflection surveying is one of the most widely used and effective techniques for coal seam structure delineation and risk mitigation for underground longwall mining. However, the ability of the method can be compromised by the presence of volcanic cover. This problem arises within parts of the Bowen and Sydney Basins of Australia and seismic surveying can be unsuccessful. As a consequence, such areas are less attractive for coal mining. Techniques to improve the success of seismic surveying over basalt flows are needed. In this paper, we use elastic wave-equation-based forward modelling techniques to investigate the effects and characteristics of seismic wave propagation under different settings involving changes in basalt properties, its thickness, lateral extent, relative position to the shot position and various forms of inhomogeneity. The modelling results suggests that: 1) basalts with high impedance contrasts and multiple flows generate strong multiples and weak reflectors; 2) thin basalts have less effect than thick basalts; 3) partial basalt cover has less effect than full basalt cover; 4) low frequency seismic waves (especially at large offsets) have better penetration through the basalt than high frequency waves; and 5) the deeper the coal seams are below basalts of limited extent, the less influence the basalts will have on the wave propagation. In addition to providing insights into the issues that arise when seismic surveying under basalts, these observations suggest that careful management of seismic noise and the acquisition of long-offset seismic data with low-frequency geophones have the potential to improve the seismic results.

Land-Cover Change Detection of Western DMZ and Vicinity using Spectral Mixture Analysis of Landsat Imagery (선형분광혼합화소분석을 이용한 서부지역 DMZ의 토지피복 변화 탐지)

  • Kim, Sang-Wook
    • Journal of the Korean Association of Geographic Information Studies
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    • v.9 no.1
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    • pp.158-167
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    • 2006
  • The object of this study is to detect of land-cover change in western DMZ and vicinity. This was performed as a basic study to construct a decision support system for the conservation or a sustainable development of the DMZ and Vicinity near future. DMZ is an is 4km wide and 250km long and it's one of the most highly fortified boundaries in the world and also a unique thin green line. Environmentalists want to declare the DMZ as a natural reserve and a biodiversity zone, but nowadays through the strengthening of the inter-Korean economic cooperation, some developers are trying to construct a new-town or an industrial complex inside of the DMZ. This study investigates the current environmental conditions, especially deforestation of the western DMZ adopting remote sensing and GIS techniques. The Land-covers were identified through the linear spectvral mixture analysis(LSMA) which was used to handle the spectral mixture problem of low spatial resolution imagery of Landsat TM and ETM+ imagery. To analyze quantitative and spatial change of vegetation-cover in western DMZ, GIS overlay method was used. In LSMA, to develop high-quality fraction images, three endmembers of green vegetation(GV), soil, water were driven from pure features in the imagery. Through 15 years, from 1987 to 2002, forest of western DMZ and vicinity was devastated and changed to urban, farmland or barren land. Northern part of western DMZ and vicinity was more deforested than that of southern part. ($52.37km^2$ of North Korean forest and $39.04km^2$ of South Korean were change to other land-covers.) In case of North Korean part, forest changed to barren land and farmland and in South Korean part, forest changed to farmland and urban area. Especially, In North Korean part of DMZ and vicinity, $56.15km^2$ of farmland changed to barren land through 15 years, which showed the failure of the 'Darakbat' (terrace filed) project which is one of food increase projects in North Korea.

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Pedogenesis of Forest Soils(Kandiustalfs) Derived from Granite Gneiss in Southern Part of Korea (우리나라 남부지역(南部地域) 화강편마암질(花崗片麻巖質) 삼림토양(森林土壤)의 토양생성(土壤生成))

  • Cho, Hi Doo
    • Journal of Korean Society of Forest Science
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    • v.86 no.2
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    • pp.186-199
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    • 1997
  • The soils derived from granite gneiss occupy almost one third of the land area in Korea. The soils under forest vegetation, formed on granite gneiss, in Sun chon-shi, Chollanam-do in southern part of Korea, were studied to evaluate the weathering and the transformation of primary minerals into secondary minerals, clay minerals. The studied soils contained large amounts of ferromagnesian minerals, weathered biotites and were well weathered, strongly acid and low in organic matters and in ration exchange capacity. The clay contents in the Bt horizon were almost two times higher than those in the C horizon. The O horizon had a thin layer which consisted of a little decomposed plant components with a granic fabric and high porosity, and showed the micromorphological characteristics of moder humus. The related distribution pattern of the E horizon were enaulic and large amounts of silts and small amounts of sand grains were another characteristics of the E horizon. The most striking micromorphological features were multilaminated clay coating and infillings in the voids in the Bt and C horizons, and generally limpid ferriargillans ejected from the biotites and imparted red color to the soils in the Bt horizon. High clay contents in the Bt horizon was not only due to clay translocation, but also due to intensive in situ mineral weathering in this horizon. The most significant pedogenic process, revealed by the petrographic microscope and SEM, was the formation of iron oxides from biotites, the formation of tubular halloysites and the weathering models of biotites; wedge weathering and layer weathering. The thick coating on the weathering biotites showed the characteristics of the weathering process and the synthetic hematites were revealed in clays by TEM. Total chemical analysis of clays revealed extensive loss of Ca, and Na and the concentration of Fe and Al. Mineralogical studies of clays by XRD showed that micas were almost completely weathered to kaolinite, vermiculite-kaolinite intergrade, hematite, gibbsite, while halloysites from other primary minerals. Some dioctahedral mica appeared to be resistant in the soils. Parent rock of the soils contained a considerable amounts of biotites and this forest soils showed especially a dominant characteristics of biotite weathering.

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Feasibility study of the usefulness of SRS thermoplastic mask for head & neck cancer in tomotherapy (두경부 종양의 토모치료 시 정위적방사선수술 마스크의 유용성 평가에 대한 연구)

  • Jeon, Seong Jin;Kim, Chul Jong;Kwon, Dong Yeol;Kim, Jong Sik
    • The Journal of Korean Society for Radiation Therapy
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    • v.26 no.2
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    • pp.355-362
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    • 2014
  • Purpose : When head&neck cancer radiation therapy, thermoplastic mask is applied for patients with fixed. The purpose of this study is to evaluate usefulness of thermoplastic mask for SRS in tomotherapy by conparison with the conventional mask. Materials and Methods : Typical mask(conventional mask, C-mask) and mask for SRS are used to fix body phantom(rando phantom) on the same iso centerline, then simulation is performed. Tomotherapy plan for orbit and salivary glands is made by treatment planning system(TPS). A thick portion and a thin portion located near the treatment target relative to the mask S-mask are defined as region of interest for surface dose dosimetry. Surface dose variation depending on the type of mask was analyzed by measuring the TPS and EBT film. Results : Surface dose variation due to the type of mask from the TPS is showed in orbit and salivary glands 0.65~2.53 Gy, 0.85~1.84 Gy, respectively. In case of EBT film, -0.2~3.46 Gy, 1.04~3.02 Gy. When applied to the S-mask, in TPS and Gafchromic EBT3 film, substrantially 4.26%, 5.82% showed maximum changing trend, respectively. Conclusion : To apply S-mask for tomotherapy, surface dose is changed, but the amount is insignificant and be useful when treatment target is close critical organs because decrease inter and intra fractional variation.

The Change of Blink Rate by Wearing Soft Contact Lens (소프트콘택트렌즈 착용에 의한 순목 횟수 변화)

  • Lee, Youn-Jin;Park, Sang-Il;Lee, Heum-Sook;Park, Mi-Jung
    • Journal of Korean Ophthalmic Optics Society
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    • v.11 no.3
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    • pp.173-179
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    • 2006
  • We investigated the change of the blink rate by wearing soft contact lens(SCL). Eight types of soft contact lenses were worn by twelve asymptomatic contact lens wearers. When wearers were worn SCLs for 15 min, the average blink rate was 20.0 blinks/min, which was a statistically significant increase compared to 13.3 blinks/min, the average blink rate of non-SCL-worn eye. After 8 hr of lens wear, the average blink rate was 24.3 blinks/min, and it was 11 blinks/min more than that of non-SCL-worn eye and 4 blinks/min more than that of 15 min SCL-worn eye. There were a little difference of the blink rate in types of lens, which the blink rate range of all lens after 15 min of lens wear was 18.1~20.9 blinks/min and that after 8 hr of lens wear was 22.9~24.9 blinks/min. When wearing A lens(vifilcon, 0.06 mm) having thin center thickness, wearers showing difference of 10.0~11.9 blinks/min between non-SCL-worn eye and 15 min SCL-worn eye reached 16.7% and it was more than that of relatively thick B lens(0.17 mm) and C lens(0.14 mm). This result suggest that the center thickness was not unique factor of the blink rate change and other factors would have synthetically influence on the blink rate change. In the case of B lens and C lens of hilafilcon material but having different center thickness and water content, wearers increasing more than 4.0 blinks/min after 8 hr of lens wear was 58.3% and 41.7%, respectively. This result have provided information that the lenses of similar materials but different center thickness and water content could cause individually the different change of the blink rate.

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A study on screeining of antibacterial herb medicines against puerperal infection-bacteria, Proteus vulgaris, Staphylococcus aures, and Enterococcus faecalis (산욕기(産褥期) 감염(感染) 유발(誘發) 세균(細菌)의 생육(生育)을 억제하는 한약재(漢藥材) 탐색(探索)에 관(關)한 연구(硏究))

  • Lim, Je-Yeon;Seong, Yeon-Su;Kim, Hee-Jin;Lee, Tae-Gyun
    • The Journal of Dong Guk Oriental Medicine
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    • v.6 no.1
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    • pp.177-203
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    • 1997
  • Various kinds of medicinal herbs and prescribed herb medicines which have usually been used for treatment of reducing fever, purging intense heat, and detoxication were secreened for antibacterial activity against Proteus vulgaris, S. aureus, and E. faecalis which causing puerperal infection. Minimal inhibitory concentrations of water and ethanol-soluble extracts of Hwangyon(C. japonica), Omae(P. mume), and Omiza(S. chinensis) were determined and using thin-layer chromatography the extracts of Hwangyon and Omiza were separated. The results obtained were as follow : 1. The water-soluble extracts of Keumeunhwa(L. japonica), Chiza(G. jasminoides), Hwangyon(C. japonica), Dansam(S. miltiorrhiza), Omae(P. mume) and Omiza(S. chinensis) showed antibacterial activities against P. vulgaris ; among them, the extracts of Hwangyon, Omae, and Omiza showed high antibacterial activities. The water-soluble extracts of Keumeunhwa(L. japonica), Hwangyon(C. japonica), Hwangkeum(S. baicalensis), Hwangbaek(P. amurense), Dansam(S. miltiorrhiza), Daehwang(R. coreanum), Omae(P. mume), Omiza(S. chinensis), Gingseng(P. ginseng), and Gamcho(G. uralensis) showed antibacterial activities against S. aureus : among them, the extracts of Hwangyon and Omiza showed high antibacterial activities. The water-soluble extracts of Banggi(C. trilobus), Daehwang(R. coreanum), Omae(P. mume), Omiza(S. chinensis), and Gamcho(G. uralensis) inhibited the cell growth of E. faecalis : among them, Omae and Omiza showed high antibacterial activities. The extract of Hwangyon(C. Japonica) and Omae(P. mume) showed no inhibition against E. coli. However, the extract of Omiza(S. chinensis) inhibited cell growth of both E. coli and B. subtilis. 2. The water- and ethanol-soluble extract of Hwangyon(C. Japonica) showed antibacterial activities against P. vulgaris and S. aureus, those of Omae(P. mume) against P. vulgaris and E. faecalis, and those of Omiza(S. chinensis) against all species tested, P. vulgaris, S. aureus, and E. faecalis. With the exception that ethanol-soluble extract of Hwangyon(C. japonica) showed much higher antibacterial activity against S. aureus than water-soluble one, antibacterial activities of both water-and ethanol-soluble ones were similar to each other of other two medicinal herbs. 3. When the prescribed herb medicines were tested, Sambohwan showed antibacterial activities against P. vulgaris and S. aureus. No prescribed herb medicine inhibitied the cell growth of E. faecalis. 4. Minimal inhibitory concentrations(MICs) of both water- and ethanol-soluble extracts of Hwangyon(C. japonica), Omae(P. mume), and Omiza(S. chinensis) against P. vulgaris were $2.5mg/m{\ell}$, $10mm{\ell}$, and $20mg/m{\ell}$, respectively. Those of both Hwangyon(C. japonica) and Omiza(S. chinensis) against S. aureus were $1.25mg/m{\ell}$ and $10mg/m{\ell}$, respectively. MICs of water-soluble extracts of Omae(P. mume) and Omiza(S. chinensis) against E. faecalis were $2.5m{\ell}$ and $5m{\ell}$, and those of ethanol-soluble extracts were $5m{\ell}$ and $10m{\ell}$, respectively Except for those of E. faecalis the cell growth of P. vulgaris and S. aureus were inhibited by much lower concentration of ethanol-soluble extracts used. As a result, the antibacterial compounds against P. vulgaris S. aureus, and E. aecalis are contained in the extracts of Hwangyon(C. japonica), Omae(P. mume), Omiza(S. chinensis), the prescribed herb medicine, Sambohwan, and might be used for treatment of puerperal infection. Further study should be carried out to identify which compounds affect the cell growth inhibition of P. vulgaris S. aureus, and E. faecalis.

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Assessment of Carsington Dam Failure by Slope Stability and Dam Behavior Analyses (사면안정 해석과 댐 거동분석을 통한 Carsington Dam 파괴의 고찰)

  • 송정락;김성인
    • Proceedings of the Korean Geotechical Society Conference
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    • 1991.10a
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    • pp.87-102
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    • 1991
  • It has been reported that the failure of Carsington Dam in Eng1and occured due to the existence of a thin yellow clay layer which was not identified during the design work, and due to pre-existing shears of the clay layer. The slope stability analyses during the design work, which utilized traditional circular arc type failure method and neglected the existence of the clay layer, showed a safety factor of 1.4. However, the post-failure analyses which utilized translational failure mode considering the clay layer and the pre-existing shear deformation revealed the reduction of safety factor to unity. The post-failure analysis assumed 10。 inclination of the horizontal forces onto each slice based on the results of finite element analyses. In this paper, Bishop's simplified method, Janbu method, and Morgenstern-Price method were used for the comparison of both circular and translational failure analysis methods. The effects of the pre-existing shears and subsquent movement were also considered by varying the soil strength parameters and the pore pressure ratio according to the given soi1 parameters. The results showed factor of safefy 1.387 by Bishop's simplified method(STABL) which assumed circular arc failure surface and disregarding yellow clay layer and pre-failure material properties. Also the results showed factor of safety 1.093 by Janbu method(STABL) and 0.969 by Morgenstern-Price method(MALE) which assumed wedge failure surface and considerd yellow clay layer using post failure material properties. In addition, dam behavior was simulated by Cam-Clay model FEM program. The effects of pore pressure changes with loading and consolidation, and strength reduction near or at failure were also considered based on properly assumed stress-strain relationship and pore pressure characteristics. The results showed that the failure was initiated at the yellow clay layer and propagated through other zones by showing that stress and displacement were concentrated at the yel1ow clay layer.

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